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Journal of Food Protection Apr 2023In Mexico, bullfrogs (Lithobates catesbeianus) are produced as gourmet food. However, bullfrogs can be carriers of pathogens because the frogs' preferred living...
In Mexico, bullfrogs (Lithobates catesbeianus) are produced as gourmet food. However, bullfrogs can be carriers of pathogens because the frogs' preferred living conditions occur in stagnant water. The present study aimed to identify bacteria that cause foodborne diseases or are associated with human diseases. For molecular identification, based on the sequential analysis by 16S rRNA or rpoD was conducted on all isolates obtained from bullfrog. A total of 91 bacterial isolates were obtained from bullfrogs; 14 genera and 23 species were identified, including Acinetobacter johnsonii 16.5%; Aeromonas media 14.3%; Aeromonas veronii 13.2%; Providencia rettgeri 7.7%; Citrobacter freundii 6.6%; Aeromonas caviae 4.4%; Aeromonas hydrophila and Elizabethkingia ursingii 3.3%; Pseudomonas stutzeri, Raoultella ornithinolytica, and Shewanella putrefaciens 2.2%; Acinetobacter guillouiae, Acinetobacter pseudolwoffii, Citrobacter portucalensis, Citrobacter werkmanii, Edwardsiella anguillarum, Klebsiella michiganensis, Kluyvera intermedia, Kocuria rosea, Myroides odoratimimus, Myroides odoratus, Proteus sp., and Proteus hauseri 1.1%. In this study, 49.4% of the isolates obtained cause foodborne disease, 19.8% are bacteria that play an important role in the spoilage of food, 5.5% of isolates have nosocomial significance, 13.2% of bacteria are considered to be pollutants of the ecosystem, and in the case of A. salmonicida and Edwardsiella anguillarum (12.1%) to have a negative impact on aquaculture. Acinetobacter pseudolwoffii and Citrobacter portucalensis have not been reported to cause disease. Lastly of these isolates, 97.8% (89/91) can cause disease by food consumption or by direct contact for immunocompromised persons. The presence of these bacteria in bullfrogs represents a significant problem for human health. There is evidence that these microorganisms are pathogenic and frogs may also be reservoirs.
Topics: Animals; Humans; Rana catesbeiana; Ecosystem; RNA, Ribosomal, 16S; Foodborne Diseases
PubMed: 36948016
DOI: 10.1016/j.jfp.2023.100067 -
Polish Journal of Microbiology Mar 2023The present study was aimed to obtain a close insight into the distribution and diversity of antibiotic-resistant bacteria (ARB) and antibiotic-resistance genes (ARGs)...
The present study was aimed to obtain a close insight into the distribution and diversity of antibiotic-resistant bacteria (ARB) and antibiotic-resistance genes (ARGs) among the aquatic products collected in Zhejiang, China. A total of 136 presumptive ARB picked up from six aquatic samples were classified into 22 genera and 49 species based on the 16S rDNA sequencing. spp., spp., spp., spp., spp., and spp. accounted for 80% of the ARB. Among them, 109 isolates (80.15%) exhibited resistance to at least one antibiotic. Most isolates showed resistance to not only the originally selected drug but also to one to three other tested drugs. The diversity of ARB distributed in different aquatic products was significant. Furthermore, the resistance data obtained from genotypic tests were not entirely consistent with the results of the phenotypic evaluation. The genes S, A, R, and A were frequently detected in their corresponding phenotypic resistant isolates. In contrast, the genes 2, (6')-Ib, and were less frequently found in the corresponding phenotypically resistant strains. The high diversity and detection rate of ARB and ARGs in aquaculture might be a significant threat to the food chains closely related to human health.
Topics: Humans; Genes, Bacterial; Angiotensin Receptor Antagonists; Angiotensin-Converting Enzyme Inhibitors; Drug Resistance, Microbial; Aeromonas; Anti-Bacterial Agents
PubMed: 36929890
DOI: 10.33073/pjm-2023-010 -
Food Science & Nutrition Feb 2023The aim of this study was to investigate the sensory indicators, biogenic amine contents, and bacterial community structure and diversity of chilled chicken stored at... (Review)
Review
The aim of this study was to investigate the sensory indicators, biogenic amine contents, and bacterial community structure and diversity of chilled chicken stored at 4°C under aerobic conditions. Bacterial diversity and dominant bacteria were analyzed using high-throughput sequencing technique (HTS). The relationship between biogenic amine contents and microbial community structure was studied. The results showed that contents of putrescine and cadaverine increased significantly with storage time. Proteobacteria was absolutely dominant flora at the phylum level. The predominant spoilage bacteria found in chicken thighs were , , , , and , and the difference with chicken breasts was related to the presence of and absence of . , , and were reported for the first time as an important contributor to the spoilage-related microflora Bacterial diversity and richness indices showed fluctuating and decreasing trend with storage time. The redundancy analysis showed that the relative abundance of , , and was positively related to the contents of putrescine, cadaverine, and tyramine, while and showed positive relationship with putrescine content. Furthermore, positive relationship of and with the contents of cadaverine and tyramine was proposed for the first time. The key findings of this study can provide experimental data for food safety monitoring during refrigerated storage and preservation for poultry meat products.
PubMed: 36789075
DOI: 10.1002/fsn3.3122 -
Antonie Van Leeuwenhoek Mar 2023The strain M-43 was isolated from the Oncorhynchus mykiss from a fish farm in Mugla, Turkey. Pairwise 16S rRNA gene sequence analysis was used to identify strain M-43....
The strain M-43 was isolated from the Oncorhynchus mykiss from a fish farm in Mugla, Turkey. Pairwise 16S rRNA gene sequence analysis was used to identify strain M-43. The strain was a member of the genus Myroides sharing the highest 16S rRNA gene sequence identity levels of 98.7%, 98.3%, and 98.3% with the type strains of M. profundi D25, M. odoratimimus subsp. odoratimimus CCUG 39352 and M. odoratimimus subsp. xuanwuensis DSM27251, respectively. A polyphasic taxonomic approach including whole genome-based analyses was employed to confirm the taxonomic provenance of strain M-43 within the genus Myroides. The overall genome relatedness indices (OGRI) for strain M-43 compared with its most closely related type strains M. odoratimimus subsp. xuanwuensis DSM 27251, M. profundi D25, and M. odoratimimus subsp. odoratimimus ATCC BAA-634, were calculated as 25.3%, 25.1%, and 25% for digital DNA-DNA hybridization (dDDH), 83.3%, 83.6%, and 83.4% for average nucleotide identity (ANI) analyses, respectively. The OGRI values between strain M-43 and its close neighbors confirmed that the strain represents a novel species in the genus Myroides. The DNA G + C content of the strain is 33.7%. The major fatty acids are iso-C and summed feature 9 (iso-C ω9c and/or 10-methyl C). The predominant polar lipids are phosphatidylethanolamine, an amino-lipid and five unidentified lipids. The major respiratory quinone is MK-6. Chemotaxonomic and phylogenomic analyses of this isolate confirmed that the strain represents a novel species for which the name Myroides oncorhynchi sp. nov. is proposed, with M-43 as the type strain (JCM 34205 = KCTC 82265).
Topics: Animals; Oncorhynchus mykiss; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Flavobacteriaceae; Bacteria, Aerobic; Fatty Acids; Genomics; Phylogeny; DNA, Bacterial; Bacterial Typing Techniques
PubMed: 36586048
DOI: 10.1007/s10482-022-01804-x -
Frontiers in Microbiology 2022In this study, we identified and characterized a novel chromosomally-encoded class B metallo-β-lactamase (MBL) gene designated in a carbapenem-resistant isolate P34...
In this study, we identified and characterized a novel chromosomally-encoded class B metallo-β-lactamase (MBL) gene designated in a carbapenem-resistant isolate P34 isolated from sewage discharged from an animal farm. Comparative analysis of the deduced amino acid sequence revealed that WUS-1 shares the highest amino acid similarities with the function-characterized MBLs MUS-1 (AAN63647.1; 70.73%) and TUS-1 (AAN63648.1; 70.32%). The recombinant carrying exhibited increased MICs levels against a number of β-lactam antimicrobials such as carbenicillin, ampicillin and imipenem, and β-lactamase inhibitors (clavulanic acid and tazobactam). The metallo-β-lactamase WUS-1 could also hydrolyze these antimicrobials and the hydrolytic activities could be inhibited by EDTA. Genetic context analysis of revealed that no mobile genetic element was found in its surrounding region. The plasmid pMA84474 of P34 harbored 6 resistance genes ( , , , , and ) within an approximately 17 kb multidrug resistance (MDR) region. These genes, however, were all related to mobile genetic elements.
PubMed: 36532482
DOI: 10.3389/fmicb.2022.1059997 -
Plants (Basel, Switzerland) Nov 2022The aim of our research was to study the endosphere of four halophytic plants: L., (R.Br.), (Pall.) and (L.) Schrad. from arid and saline areas of the Stavropol...
The aim of our research was to study the endosphere of four halophytic plants: L., (R.Br.), (Pall.) and (L.) Schrad. from arid and saline areas of the Stavropol Territory, Russia. In total, 28 endophyte strains were isolated from the roots and stems of these halophytic plants. Most of the isolates (23 out of 28) were identified as sp. while others belonged to the genera and . Three strains of sp. (Se5R, Se1-1R, and Se1-3S), isolated from the were capable of growth at 55 °C and in 10% of NaCl. Strains Se1-4S, Kp20-2S, and Bs11-2S sp. (isolated from the , and respectively) demonstrated strong plant growth promoting activity: 85-265% over control lettuce plants and a high degree of growth suppression (59.1-81.2%) of pathogenic fungi , and . Selected strains can be promising candidates for the development of bioinoculants to facilitate salt soil phytoremediation and be beneficial for mitigating the salt stress to the plants growing in salt-affected habitats.
PubMed: 36365445
DOI: 10.3390/plants11212992 -
Revista Espanola de Quimioterapia :... Dec 2022
Topics: Humans; Flavobacteriaceae; Urinary Tract Infections
PubMed: 36183239
DOI: 10.37201/req/045.2022 -
Foods (Basel, Switzerland) Aug 2022Storage temperature is considered one of the most important factors that affect the microbial spoilage of fresh meat. Chilling and superchilling are the most popular...
Storage temperature is considered one of the most important factors that affect the microbial spoilage of fresh meat. Chilling and superchilling are the most popular storage techniques on the market, but during transportation, the temperature may reach 10 °C and may even reach room temperature during local retail storage. In the present study, we stored fresh pork meat at different temperatures, -2 °C, 4 °C, 10 °C, and 25 °C. The composition and functional potential of fresh or spoiled meat resident microbes were analyzed based on 16S rRNA gene amplicon sequencing. The microbial composition exhibited high similarity between pork meat stored at -2 °C and 4 °C, with and being the dominant taxa. sp., sp., and sp. were markers for spoiled pork meat stored at 25 °C. Both psychrophilic and mesophilic bacteria were observed to grow under a storage temperature of 10 °C, but the overall composition and functional potential based on Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were found to be similar to that of meat stored at room temperature. Our results broaden the knowledge of possible microbial changes in pork meat during storage, transportation, or retail.
PubMed: 35954075
DOI: 10.3390/foods11152307 -
Microbial Biotechnology Oct 2022Black soldier fly (BSF) larvae are considered a promising biological reactor to convert organic waste and reduce the impact of zoonotic pathogens on the environment. We...
Black soldier fly (BSF) larvae are considered a promising biological reactor to convert organic waste and reduce the impact of zoonotic pathogens on the environment. We analysed the effects of BSF larvae on Staphylococcus aureus and Salmonella spp. populations in pig manure (PM), which showed that BSF larvae can significantly reduce the counts of the associated S. aureus and Salmonella spp. Then, using a sterile BSF larval system, we validated the function of BSF larval intestinal microbiota in vivo to suppress pathogens, and lastly, we isolated eight bacterial strains from the BSF larval gut that inhibit S. aureus. Results indicated that functional microbes are essential for BSF larvae to antagonise S. aureus. Moreover, the analysis results of the relationship between the intestinal microbiota and S. aureus and Salmonella spp. showed that Myroides, Tissierella, Oblitimonas, Paenalcalignes, Terrisporobacter, Clostridium, Fastidiosipila, Pseudomonas, Ignatzschineria, Savagea, Moheibacter and Sphingobacterium were negatively correlated with S. aureus and Salmonella. Overall, these results suggested that the potential ability of BSF larvae to inhibit S. aureus and Salmonella spp. present in PM is accomplished primarily by gut-associated microorganisms.
Topics: Animals; Diptera; Gastrointestinal Microbiome; Larva; Manure; Staphylococcus aureus; Swine
PubMed: 35881487
DOI: 10.1111/1751-7915.14113 -
Case Reports in Infectious Diseases 2022The bacterial genus , like other members of the family, consists of aerobic, non-motile, Gram-negative bacilli. spp. is considered predominantly opportunistic...
The bacterial genus , like other members of the family, consists of aerobic, non-motile, Gram-negative bacilli. spp. is considered predominantly opportunistic pathogens as, historically, most documented infections have been in immunocompromised individuals. Along with advancements in molecular assay testing, there are growing reports of clinically relevant spp. infections in immunocompetent individuals. These organisms display broad antimicrobial resistance, and while research into their mechanisms of resistance is progressing, genetic testing has revealed metallo--lactamases present in their genome. The sporadic identification of spp. and ongoing clarification of resistance patterns make empiric treatment difficult. This report documents two cases of extensively drug-resistant isolated from critically ill but otherwise immunocompetent patients followed by a review of available literature on spp. antibiotic sensitivities. Our findings indicate that minocycline and moxifloxacin have the highest documented activity against spp.
PubMed: 35873406
DOI: 10.1155/2022/6422861