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Clinical Case Reports Jun 2024Myroides odoratus infection, a rare gram-negative bacillus, predominantly affects immunocompromised patients and presents with diverse clinical manifestations....
Myroides odoratus infection, a rare gram-negative bacillus, predominantly affects immunocompromised patients and presents with diverse clinical manifestations. Antibiotic resistance poses challenges in determining appropriate treatment. This case underscores successful management of Myroides odoratus cellulitis complicated by bacteremia with dual antibiotic therapy, emphasizing the importance of timely recognition and susceptibility testing for effective treatment strategies.
PubMed: 38887306
DOI: 10.1002/ccr3.9090 -
The Journal of Hospital Infection Jul 2023Myroides spp. are Gram-negative bacilli that are commonly found in soil and water, acting as low-level opportunistic pathogens and causing a variety of infections.
BACKGROUND
Myroides spp. are Gram-negative bacilli that are commonly found in soil and water, acting as low-level opportunistic pathogens and causing a variety of infections.
AIM
To assess the risk factors for multi-drug-resistant myroides infections, association with comorbid illnesses, patient care and susceptibility to antibiotics.
METHODS
This retrospective analytical study was conducted in Istanbul Başakşehir Çam and Sakura City Hospital, and included patients with Myroides spp. isolated in their culture samples. Total hospitalization days, first isolation day and 30-day mortality values of the patients were analysed statistically, and P<0.05 was considered to indicate significance.
RESULTS
Myroides spp. were isolated from 437 culture samples from 228 patients. Of these cases, 210 (92.1%) were classified as having asymptomatic bacteriuria, and 18 (7.9%) as having an infection caused by Myroides spp. One hundred and seventy-four (76.3%) patients were followed up in the intensive care unit, and total hospitalization days (median 24.5 days) and first isolation day (median 9.5 days) of infected patients were shorter than those for colonized patients (P=0.023 and 0.030, respectively). No difference was found in 30-day mortality between infected and colonized patients (P=0.312).
CONCLUSIONS
Myroides infections were seen more frequently in patients who were hospitalized for a long time, used broad-spectrum antimicrobials, underwent invasive procedures, and had co-factors such as diabetes and cerebrovascular disease. In addition, the resistance rates of Myroides odoratus were higher than those of Myroides odoratimimus, and the use of quinolones for the treatment of patients with M. odoratimimus infection led to a higher cure rate.
Topics: Humans; Retrospective Studies; Gram-Negative Bacteria; Anti-Bacterial Agents; Bacteria, Aerobic; Risk Factors; Intensive Care Units
PubMed: 37094716
DOI: 10.1016/j.jhin.2023.04.010 -
Journal of Food Protection Apr 2023In Mexico, bullfrogs (Lithobates catesbeianus) are produced as gourmet food. However, bullfrogs can be carriers of pathogens because the frogs' preferred living...
In Mexico, bullfrogs (Lithobates catesbeianus) are produced as gourmet food. However, bullfrogs can be carriers of pathogens because the frogs' preferred living conditions occur in stagnant water. The present study aimed to identify bacteria that cause foodborne diseases or are associated with human diseases. For molecular identification, based on the sequential analysis by 16S rRNA or rpoD was conducted on all isolates obtained from bullfrog. A total of 91 bacterial isolates were obtained from bullfrogs; 14 genera and 23 species were identified, including Acinetobacter johnsonii 16.5%; Aeromonas media 14.3%; Aeromonas veronii 13.2%; Providencia rettgeri 7.7%; Citrobacter freundii 6.6%; Aeromonas caviae 4.4%; Aeromonas hydrophila and Elizabethkingia ursingii 3.3%; Pseudomonas stutzeri, Raoultella ornithinolytica, and Shewanella putrefaciens 2.2%; Acinetobacter guillouiae, Acinetobacter pseudolwoffii, Citrobacter portucalensis, Citrobacter werkmanii, Edwardsiella anguillarum, Klebsiella michiganensis, Kluyvera intermedia, Kocuria rosea, Myroides odoratimimus, Myroides odoratus, Proteus sp., and Proteus hauseri 1.1%. In this study, 49.4% of the isolates obtained cause foodborne disease, 19.8% are bacteria that play an important role in the spoilage of food, 5.5% of isolates have nosocomial significance, 13.2% of bacteria are considered to be pollutants of the ecosystem, and in the case of A. salmonicida and Edwardsiella anguillarum (12.1%) to have a negative impact on aquaculture. Acinetobacter pseudolwoffii and Citrobacter portucalensis have not been reported to cause disease. Lastly of these isolates, 97.8% (89/91) can cause disease by food consumption or by direct contact for immunocompromised persons. The presence of these bacteria in bullfrogs represents a significant problem for human health. There is evidence that these microorganisms are pathogenic and frogs may also be reservoirs.
Topics: Animals; Humans; Rana catesbeiana; Ecosystem; RNA, Ribosomal, 16S; Foodborne Diseases
PubMed: 36948016
DOI: 10.1016/j.jfp.2023.100067 -
Revista Espanola de Quimioterapia :... Dec 2022
Topics: Humans; Flavobacteriaceae; Urinary Tract Infections
PubMed: 36183239
DOI: 10.37201/req/045.2022 -
Case Reports in Infectious Diseases 2022The bacterial genus , like other members of the family, consists of aerobic, non-motile, Gram-negative bacilli. spp. is considered predominantly opportunistic...
The bacterial genus , like other members of the family, consists of aerobic, non-motile, Gram-negative bacilli. spp. is considered predominantly opportunistic pathogens as, historically, most documented infections have been in immunocompromised individuals. Along with advancements in molecular assay testing, there are growing reports of clinically relevant spp. infections in immunocompetent individuals. These organisms display broad antimicrobial resistance, and while research into their mechanisms of resistance is progressing, genetic testing has revealed metallo--lactamases present in their genome. The sporadic identification of spp. and ongoing clarification of resistance patterns make empiric treatment difficult. This report documents two cases of extensively drug-resistant isolated from critically ill but otherwise immunocompetent patients followed by a review of available literature on spp. antibiotic sensitivities. Our findings indicate that minocycline and moxifloxacin have the highest documented activity against spp.
PubMed: 35873406
DOI: 10.1155/2022/6422861 -
Journal of Thoracic Disease Feb 2022The current COVID-19 pandemic is posing a major challenge to public health on a global scale. While it is generally believed that severe COVID-19 results from...
BACKGROUND
The current COVID-19 pandemic is posing a major challenge to public health on a global scale. While it is generally believed that severe COVID-19 results from over-expression of inflammatory mediators (i.e., a "cytokine storm"), it is still unclear whether and how co-infecting pathogens contribute to disease pathogenesis. To address this, we followed the entire course of the disease in cases with severe or critical COVID-19 to determine the presence and abundance of all potential pathogens present-the total "infectome"-and how they interact with the host immune system in the context of severe COVID-19.
METHODS
We examined one severe and three critical cases of COVID-19, as well as a set of healthy controls, with longitudinal samples (throat swab, whole blood, and serum) collected from each case. Total RNA sequencing (meta-transcriptomics) was performed to simultaneously investigate pathogen diversity and abundance, as well as host immune responses, in each sample. A Bio-Plex method was used to measure serum cytokine and chemokine levels.
RESULTS
Eight pathogens, SARS-CoV-2, (), (), (), (), , herpes simplex virus (HSV) and human cytomegalovirus (CMV), identified in patients with COVID-19 appeared at different stages of the disease. The dynamics of inflammatory mediators in serum and the respiratory tract were more strongly associated with the dynamics of the infectome compared with SARS-CoV-2 alone. Correlation analysis revealed that pulmonary injury was directly associated with cytokine levels, which in turn were associated with the proliferation of SARS-CoV-2 and co-infecting pathogens.
CONCLUSIONS
For each patient, the cytokine storm that resulted in acute lung injury and death involved a dynamic and highly complex infectome, of which SARS-CoV-2 was a component. These results indicate the need for a precision medicine approach to investigate both the infection and host response as a standard means of infectious disease characterization.
PubMed: 35280492
DOI: 10.21037/jtd-21-1284 -
International Journal of Systematic and... Feb 2021A Gram-stain-negative, aerobic, short rod-shaped, pale yellow-pigmented, non-motile and gentamycin-resistant bacterial strain designated CJ210 was isolated from the Han...
A Gram-stain-negative, aerobic, short rod-shaped, pale yellow-pigmented, non-motile and gentamycin-resistant bacterial strain designated CJ210 was isolated from the Han River, Republic of Korea. Strain CJ210 grew optimally at 30 °C and pH 7.0 in the absence of NaCl on tryptic soy agar. Flexirubin-type pigments were not produced. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain CJ210 belonged to the genus within the family and was most closely related to KACC 14347 (98.1 % similarity), followed by KCTC 23367 (95.3 % similarity). The average nucleotide identity values between strain CJ210 and two closely related type strains KACC 14347 and KCTC 23367 were 83.7 and 73.8 %, respectively. The digital DNA-DNA hybridization results between strain CJ210 and the related type strains were 27.5 and 20.2 %, respectively. Strain CJ210 contained menaquinone 6 (MK-6) as the predominant menaquinone. The predominant polar lipids were phosphatidylethanolamine, two unidentified aminolipids and two unidentified lipids. The major fatty acids of strain CJ210 were iso-C, iso-C 3-OH and summed feature 9 (comprising iso-C 9 and/or C 10-methyl). Whole genome sequencing revealed that strain CJ210 had a genome of 3.8 Mbp with 36.5 % DNA G+C content. The genome contained several antimicrobial resistance genes including an aminoglycoside-resistant gene. On the basis of the polyphasic taxonomic study, strain CJ210 represents a novel species in the genus , for which name sp. nov. is proposed. The type strain is CJ210 (=KACC 19954=JCM 33306).
Topics: Bacterial Typing Techniques; Base Composition; DNA, Bacterial; Fatty Acids; Flavobacteriaceae; Nucleic Acid Hybridization; Phosphatidylethanolamines; Phylogeny; Pigmentation; RNA, Ribosomal, 16S; Republic of Korea; Rivers; Sequence Analysis, DNA; Vitamin K 2
PubMed: 33464202
DOI: 10.1099/ijsem.0.004623 -
Emerging Microbes & Infections Apr 2018The genus Myroides comprises several species of Gram-negative, non-motile, and non-fermenting bacteria, which have been regarded as non-pathogenic for decades. Multiple...
The genus Myroides comprises several species of Gram-negative, non-motile, and non-fermenting bacteria, which have been regarded as non-pathogenic for decades. Multiple recent reports, however, underscore the pathogenic potential that Myroides sp. possesses for humans. These bacteria seem to be resistant to a wide range of antibiotics (including ß-lactams and aminoglycosides). Therefore, treatment options are limited. Knowledge of antimicrobial resistance, however, is based on only one meaningful comprehensive study and on data published from case reports. This lack of data motivated us to test 59 strains from our Myroides collection (43 M. odoratimimus and 16 M. odoratus) for resistance against 20 commonly used antibiotics. We also performed molecular analyses to reveal whether our bacteria harbor the genus-specific M. odoratimimus metallo-ß-lactamase (MUS-1) or the M. odoratus metallo ß-lactamase (TUS-1), and other ß-lactamases, which may provide an explanation for the extended antimicrobial resistance.
Topics: Anti-Bacterial Agents; DNA, Bacterial; Flavobacteriaceae; Humans; Microbial Sensitivity Tests; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Whole Genome Sequencing; beta-Lactamases
PubMed: 29618738
DOI: 10.1038/s41426-018-0061-x -
Microbial Drug Resistance (Larchmont,... Sep 2018Myroides odoratus is a low-virulence opportunistic human pathogen. Infections caused by M. odoratus are not common, but reports are increasing in recent years. The...
Myroides odoratus is a low-virulence opportunistic human pathogen. Infections caused by M. odoratus are not common, but reports are increasing in recent years. The biggest challenge for treatment is its resistance to most antibiotics. In 2015, we isolated a pathogenic multidrug-resistant strain of M. odoratus from a urinary tract infection (UTI) patient's urine sample. To report the experience in managing M. odoratus-related UTI and investigate the genetic mechanism of this carbapenem-resistant strain, we conducted a series of microbiological and molecular studies. The bacterial strain was identified as M. odoratus by 16S rRNA gene sequencing. The minimum inhibitory concentrations (MICs) of 17 antimicrobial agents were determined against this strain. Whole-genome sequencing was performed and screened for possible β-lactamase genes. A β-lactamase gene, bla, was identified by whole-genome sequencing, then cloned and expressed in Escherichia coli DH5α to characterize its function. Antimicrobial susceptibility testing showed that the strain had relatively low MIC to levofloxacin and sulfamethoxazole/trimethoprim (SMZ/TMP). After unsuccessful empirical therapy, the UTI was controlled by levofloxacin in combination with SMZ/TMP. Whole-genome sequencing identified a subclass B1 metallo-β-lactamase gene, bla, which conferred resistance to most β-lactams except for aztreonam and cefepime. In conclusion, a new β-lactamase gene, bla, was found in an isolate of M. odoratus. The broad antimicrobial resistance of this isolate is at least attributed partially to this gene.
Topics: Amino Acid Sequence; Anti-Bacterial Agents; Carbapenems; Drug Resistance, Multiple, Bacterial; Escherichia coli; Flavobacteriaceae; Humans; Microbial Sensitivity Tests; RNA, Ribosomal, 16S; Sequence Alignment; Urinary Tract Infections; Whole Genome Sequencing; beta-Lactamases; beta-Lactams
PubMed: 29227741
DOI: 10.1089/mdr.2017.0233 -
Anaesthesia and Intensive Care Mar 2016
Topics: Flavobacteriaceae; Humans; Intensive Care Units; Male; Middle Aged; Pancreatitis; Shock, Septic
PubMed: 27029668
DOI: No ID Found