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Journal of Clinical and Diagnostic... Nov 2015Myroides spp., previously known as Flavobacterium odoratum, are rare clinical isolates and are often considered non-pathogenic. Natural habitat includes soil, fresh and...
Myroides spp., previously known as Flavobacterium odoratum, are rare clinical isolates and are often considered non-pathogenic. Natural habitat includes soil, fresh and marine waters, in foods and in sewage treatment plants. We present an unusual case of fatal pericardial effusion due to Myroides odoratus in a patient suffering from chronic kidney disease and undergoing maintenance haemodialysis. This case is presented to show the increasing incidence of rare isolates causing localized and systemic infections and due to their high intrinsic resistance to many antibiotics they can be fatal. Thus isolation of these pathogens is of great clinical importance.
PubMed: 26672889
DOI: 10.7860/JCDR/2015/15120.6740 -
International Journal of Systematic and... Nov 2015A novel aerobic, non-motile, rod-shaped, catalase- and oxidase-positive bacterial strain, designated UKS3T, was isolated from garden soil, and subjected to polyphasic...
A novel aerobic, non-motile, rod-shaped, catalase- and oxidase-positive bacterial strain, designated UKS3T, was isolated from garden soil, and subjected to polyphasic taxonomic analysis. Strain UKS3T formed whitish, viscous colonies on nutrient agar and was Gram-staining negative. Phylogenetic analysis, based on 16S rRNA gene sequence, showed that maximum pairwise similarity occurs with representatives of the genus Myroides. The most closely related species include Myroides marinus JS-08T (92.7 % sequence similarity), Myroides phaeus MY15T (92.7 %), Myroides odoratus DSM 2801T (91.5 %) and Myroides odoratimimus CCUG 39352T (91.4 %). Strain UKS3T contained menaquinone-6 (MK-6) as the major respiratory quinone and iso-C15 : 0 (40.2 %), anteiso-C15 : 0 (9.4 %) and iso-C17 : 0 3-OH (8.5 %) as major fatty acids. Phosphatidylethanolamine, phospholipids and three aminolipids were the major polar lipids. The DNA G+C content of strain UKS3T was 36.8 ± 2.0 mol%. On the basis of phenotypic, chemotaxonomic and molecular analysis, strain UKS3T represents a novel species of the genus Myroides, for which the name Myroides indicus sp. nov., is proposed. The type strain is UKS3T ( = DSM 28213T = NCIM 5555T ).
Topics: Bacteria, Aerobic; Bacterial Typing Techniques; Base Composition; DNA, Bacterial; Fatty Acids; Flavobacteriaceae; Gardens; India; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Soil Microbiology; Vitamin K 2
PubMed: 26276159
DOI: 10.1099/ijsem.0.000530 -
Antonie Van Leeuwenhoek Jan 2015A Gram-negative, yellow-pigmented, rod-shaped bacteria, designated M09-0166(T)and M09-1053 were isolated from human urine samples. 16S rRNA gene sequence analysis...
A Gram-negative, yellow-pigmented, rod-shaped bacteria, designated M09-0166(T)and M09-1053 were isolated from human urine samples. 16S rRNA gene sequence analysis revealed that the isolates belong to the Myroides cluster and were closely related to Myroides phaeus DSM 23313(T) (96.3 %), Myroides odoratimimus KCTC 23053(T) (96.1 %), Myroides profundi KCTC 23066(T) (96.0 %), Myroides odoratus KCTC 23054(T) (95.4 %) and Myroides pelagicus KCTC 12661(T) (95.2 %). The major mena quinone was identified as MK-6. The major polar lipids were identified as phosphatidylethanolamine, amino lipids, and several unknown lipids, and the major fatty acids as iso-C15:0 and iso-C17:0 3-OH. Phenotypic and chemotaxonomic data supported the affiliation of the isolates with the genus Myroides and clearly indicated that two isolates represent novel species, for which the name Myroides injenensis sp. nov. (type strain, M09-0166(T) = KCTC 23367(T) = JCM 17451(T)) is proposed.
Topics: Bacterial Typing Techniques; Cluster Analysis; Cytosol; DNA, Bacterial; DNA, Ribosomal; Fatty Acids; Flavobacteriaceae; Flavobacteriaceae Infections; Humans; Molecular Sequence Data; Phospholipids; Phylogeny; Pigments, Biological; Quinones; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Urine
PubMed: 25515411
DOI: 10.1007/s10482-014-0317-y -
Indian Journal of Medical Microbiology 2014Myroides spp and Chryseobacterium spp are uncommon clinical isolates, though more frequently reported to cause infections than other pigmented non-fermentors. Two cases...
Myroides spp and Chryseobacterium spp are uncommon clinical isolates, though more frequently reported to cause infections than other pigmented non-fermentors. Two cases of Myroides odoratus and Chryseobacterium indologenes infection in a diabetic with pulmonary tuberculosis and a patient with de-compensated alcoholic liver disease, respectively, are reported here. Anti-microbial susceptibility testing of the isolates was performed by determining the minimum inhibitory concentration. The clinical picture, characteristic features of the isolates and the antibiotic susceptibility pattern are discussed briefly.
Topics: Anti-Bacterial Agents; Chryseobacterium; Female; Flavobacteriaceae; Flavobacteriaceae Infections; Humans; Immunocompromised Host; Male; Microbial Sensitivity Tests; Middle Aged
PubMed: 25008832
DOI: 10.4103/0255-0857.136592 -
Infection Oct 2014Myroides sp., previously known as Flavobacterium odoratum, is a relatively unknown organism with unclear human pathogenicity. While Myroides sp. has been implicated in... (Review)
Review
Myroides sp., previously known as Flavobacterium odoratum, is a relatively unknown organism with unclear human pathogenicity. While Myroides sp. has been implicated in human infections, many reports have described the organism as a relatively avirulent opportunistic pathogen. We present an unusual case of rapidly fatal necrotizing fasciitis and septic shock due to Myroides odoratus. Our case demonstrates the pathogenicity of Myroides, and highlights potential risk factors for infection including underlying liver disease and open wounds. The recognition of Myroides is of particular importance given its resistance to multiple antibiotics. We review the literature on Myroides sp. skin and soft tissue infections, including necrotizing forms, and discuss the clinical presentation and management of this potentially emerging pathogen.
Topics: Anti-Bacterial Agents; California; Diagnosis, Differential; Fasciitis, Necrotizing; Fatal Outcome; Female; Flavobacteriaceae; Flavobacteriaceae Infections; Humans; Microbial Sensitivity Tests; Middle Aged; Shock, Septic
PubMed: 24806817
DOI: 10.1007/s15010-014-0626-0 -
Diagnostic Microbiology and Infectious... Jun 2014The genus Myroides comprises the 2 medically relevant species Myroides odoratus and Myroides odoratimimus that are rare opportunistic pathogens and cause infections in... (Comparative Study)
Comparative Study
The genus Myroides comprises the 2 medically relevant species Myroides odoratus and Myroides odoratimimus that are rare opportunistic pathogens and cause infections in immunocompromised patients. A fast identification of Myroides is of importance because these bacterial strains show multiple resistance against antibiotics and therefore limit treatment options. They are associated, for instance, with urinary tract infections, sepsis, meningitis, pneumonia, and infectious cellulitis. Since more and more Myroides spp. are being described, additional potentially pathogenic bacteria may be identified in the future demanding the need for fast and reliable identification methods at species level. However, to date, only molecular approaches meet these demands. In this study, we, therefore, attempt to define an appropriate method other than DNA fingerprinting that will permit a comparable efficacy and, possibly, a more economical strain identification. For this purpose, we compared 2 widely used automated diagnostic systems (VITEK 2 [bioMérieux, Nürtingen, Germany] and Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) [Bruker Daltonics, Bremen, Germany]) and correlated the results to 16S rDNA sequencing data. In total, we analyzed 22 strains collected in the course of routine diagnostics. In this study, we demonstrate that VITEK 2 reliably identifies the genus Myroides but cannot differentiate between M. odoratimimus and M. odoratus. In contrast to this, both MALDI-TOF MS and 16S rDNA sequencing efficiently distinguish between the 2 species.
Topics: Bacterial Typing Techniques; DNA, Ribosomal; Flavobacteriaceae; Flavobacteriaceae Infections; Humans; Molecular Diagnostic Techniques; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PubMed: 24666701
DOI: 10.1016/j.diagmicrobio.2014.02.002 -
Journal of Applied Microbiology Dec 2009Although Myroides spp. are primarily isolated from clinical sources, they have also been isolated from freshwater fish, stainless steel weldment and catheter biofilms....
AIMS
Although Myroides spp. are primarily isolated from clinical sources, they have also been isolated from freshwater fish, stainless steel weldment and catheter biofilms. The ability of these nonmotile isolates to adhere to surfaces and the role of hydrophobicity and autoaggregation were investigated.
METHODS
Adherence of Myroides odoratus isolates from tilapia was investigated using microtitre adherence assays under varying environmental conditions. Hydrophobicity, aggregation and coaggregation were measured quantitatively. Biofilm structures were investigated using flow cells and microscopy.
RESULTS AND CONCLUSIONS
Myroides odoratus isolates were strongly adherent in both nutrient-poor and -rich media at room temperature and nutrient-rich medium at 37 degrees C. Isolates appeared hydrophilic, while autoaggregation indices ranged from 7% to 24.8%. Statistically significant positive correlations were observed between biofilm formation and autoaggregation. Strong coaggregation interactions were observed with several Gram-positive organisms, as well as with Listeria spp. In flow cells, M. odoratus formed an overlying carpet, with knitted chains of L. monocytogenes cells situated underneath attached to the perspex flow cell surface.
SIGNIFICANCE AND IMPACT OF STUDY
Myroides odoratus biofilm formation was influenced by temperature variation rather than altered nutrient levels. The association of Myroides spp. with Listeria spp. isolates suggests potential association in biofilm structures when these bacteria colonize biotic and/or abiotic surfaces.
Topics: Animals; Bacterial Adhesion; Biofilms; Fish Diseases; Flavobacteriaceae; Flavobacteriaceae Infections; Hydrophobic and Hydrophilic Interactions; Stainless Steel; Temperature; Tilapia
PubMed: 19519668
DOI: 10.1111/j.1365-2672.2009.04380.x -
Infection Control and Hospital... Apr 2008An outbreak of central venous catheter-associated bloodstream infections was reported in a hospital in Ecuador. Commercially produced ampoules of water for injection...
An outbreak of central venous catheter-associated bloodstream infections was reported in a hospital in Ecuador. Commercially produced ampoules of water for injection were found to be contaminated with Burkholderia cepacia and Myroides odoratus. Removal of these ampoules yielded a 10-fold reduction in the incidence of catheter-associated infection.
Topics: Bacteremia; Burkholderia Infections; Burkholderia cepacia; Catheterization, Central Venous; Catheters, Indwelling; Disease Outbreaks; Ecuador; Equipment Contamination; Flavobacteriaceae; Flavobacteriaceae Infections; Hospitals, Teaching; Humans; Incidence; Retrospective Studies; Water Microbiology
PubMed: 18462150
DOI: 10.1086/533543 -
International Journal of Systematic and... Aug 2006An aerobic, Gram-negative, non-motile, yellow-to-orange pigmented and rod-shaped bacterium, designated strain SM1(T), was isolated from seawater in Thailand and...
An aerobic, Gram-negative, non-motile, yellow-to-orange pigmented and rod-shaped bacterium, designated strain SM1(T), was isolated from seawater in Thailand and subjected to a polyphasic taxonomic study. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the novel isolate shared 93-95 % sequence similarity with species of the genus Myroides. The DNA-DNA relatedness values of strain SM1(T) with Myroides odoratimimus JCM 7460(T) and Myroides odoratus JCM 7458(T) were below 70 %. The DNA G+C content of strain SM1(T) was 33.6 mol%, the major menaquinone was MK-6 and the major fatty acids were C(15 : 0) iso, C(17 : 1)omega9c iso and C(17 : 0) iso 3-OH. Based on its phenotypic and chemotaxonomic characteristics, strain SM1(T) was classified as representing a novel species of the genus Myroides, for which the name Myroides pelagicus sp. nov. is proposed. The type strain is SM1(T) (=IAM 15337(T)=KCTC 12661(T)).
Topics: Bacteroidetes; Base Composition; DNA, Bacterial; Fatty Acids; Molecular Sequence Data; Nucleic Acid Hybridization; Oceans and Seas; Phylogeny; RNA, Bacterial; RNA, Ribosomal, 16S; Sequence Homology, Nucleic Acid; Thailand; Vitamin K 2; Water Microbiology
PubMed: 16902031
DOI: 10.1099/ijs.0.64336-0 -
Scandinavian Journal of Infectious... 2006Microbiological cultures from 229 patients seeking medical advice in Stockholm after the tsunami catastrophe of December 2004 were analysed at the Clinical Microbiology...
Microbiological cultures from 229 patients seeking medical advice in Stockholm after the tsunami catastrophe of December 2004 were analysed at the Clinical Microbiology Laboratory, Karolinska University Hospital, Stockholm, Sweden. Gram-negative bacilli were the most common findings from wound cultures. Common human pathogens such as Escherichia coli, Proteus species, Klebsiella spp., and Pseudomonas aeruginosa were isolated. More rare species of Gram-negative bacilli, e.g. Myroides odoratus, Sphingomonas paucimobilis and Bergeyella zoohelcum were also isolated. Resistance towards ordinary antibiotics was more extensive compared to our Swedish reference material for Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis and Acinetobacter spp., but not for Pseudomonas aeruginosa, probably reflecting that the resistant isolates were nosocomially acquired in Asia.
Topics: Cluster Analysis; Disasters; Drug Resistance, Multiple, Bacterial; Gram-Negative Bacteria; Gram-Negative Bacterial Infections; Humans; Indian Ocean; Sweden; Wound Infection
PubMed: 16798691
DOI: 10.1080/00365540500452499