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Enzyme and Microbial Technology Jan 2020A mediator biosensor based on Paracoccus yeei bacteria for assaying the toxicity of perfumery and cosmetics samples was developed. An approach to selecting an...
A mediator biosensor based on Paracoccus yeei bacteria for assaying the toxicity of perfumery and cosmetics samples was developed. An approach to selecting an electron-transport mediator based on the heterogeneous electron transfer constants for investigated mediators (k) and the mediator-biomaterial interaction constants (k) was proposed. Screening of nine compounds as potential mediators showed a ferrocene mediator immobilized in graphite paste to have the highest efficiency of electron transfer to the graphite-paste electrode (the heterogeneous transfer constant, 0.4 ± 0.1 cm/s) and a high constant of interaction with P. yeei (0.023 ± 0.001 dm/(g·s)). A biosensor for toxicity assessment based on the ferrocene mediator and P. yeei bacteria was formed. The biosensor was tested on samples of four heavy metals (Cu, Zn, Pb, Cd) and two phenols (phenol and p-nitrophenol). Proceeding from the EC index, it was found that the use of the ferrocene mediator made the biosensor more sensitive to investigated toxicants than most analogues described. Toxicity determination of four perfumery and cosmetics samples by the developed biosensor showed prospects of using this system for real-time toxicity monitoring of samples.
Topics: Biosensing Techniques; Metals, Heavy; Nitrophenols; Paracoccus; Perfume; Phenols; Toxicity Tests
PubMed: 31731957
DOI: 10.1016/j.enzmictec.2019.109435 -
Clinical Laboratory Aug 2019Parotid cyst is a common problem in patients treated by surgeons. However, Paracoccus yeei was isolated from an aerobic blood culture in a patient with parotid cyst as...
BACKGROUND
Parotid cyst is a common problem in patients treated by surgeons. However, Paracoccus yeei was isolated from an aerobic blood culture in a patient with parotid cyst as an unusual etiologic opportunistic agent.
METHODS
Since old biochemical identification kits are not able to identify this species, MALDI-TOF MS correctly was recommended to identify this isolate. Its identity was confirmed by sequencing of the 16S rRNA gene.
RESULTS
The aligned sequences (16S rRNA gene) were used for a phylogenetic analysis (phylogenetic tree), which was produced using the BLAST pair-wise alignments. The sequence analysis determined that the best matches were with Paracoccus yeei.
CONCLUSIONS
Paracoccus yeei has been reported as a rare opportunistic human pathogen, we should actively com-municate to the clinic to improve the real positive rate.
Topics: Bacterial Typing Techniques; Cysts; DNA, Bacterial; Humans; Male; Middle Aged; Paracoccus; Parotid Diseases; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PubMed: 31414739
DOI: 10.7754/Clin.Lab.2019.190125 -
IDCases 2019is a Gram-negative coccobacilli which is often an environmental organism. However, infection of patients usually with underlying immunosuppression has been described in...
INTRODUCTION
is a Gram-negative coccobacilli which is often an environmental organism. However, infection of patients usually with underlying immunosuppression has been described in the last decades, mainly due to the emergence of diagnostic molecular methods.
CASE PRESENTATION
We describe here a case of peritonitis in a patient undergoing peritoneal dialysis. Turbidity of the peritoneal dialysate was the sole clinical manifestation. Inflammatory markers were not raised. A peritoneal fluid specimen showed increased white-cell count, but no organisms were seen on Gram stain. identified as the infectious agent. Patient was successfully treated with gentamicin. Minimum inhibitory concentration analysis suggested to be sensitive to aminoglycosides and specific betalactams but not to ciprofloxacin and ceftazidime, in line with previous literature.
DISCUSSION
This case of peritoneal-dialysis peritonitis contributes to accumulating evidence on the emergent role of this organism as a relevant human pathogen. It also provides information about antibiotic resistance patterns that helps to guide therapy more specifically and effectively.
PubMed: 30701158
DOI: 10.1016/j.idcr.2019.e00486 -
Frontiers in Microbiology 2018Bacteria of the genus are common components of the microbiomes of many naturally- and anthropogenically shaped environments. One species, , is unique within the genus...
Bacteria of the genus are common components of the microbiomes of many naturally- and anthropogenically shaped environments. One species, , is unique within the genus because it is associated with opportunistic human infections. Therefore, strains of may serve as an interesting model to study the transition from a saprophytic to a pathogenic lifestyle in environmental bacteria. Unfortunately, knowledge concerning the biology, genetics and genomic content of is fragmentary; also the mechanisms of pathogenicity of this bacterium remain unclear. In this study we provide the first insight into the genome composition and metabolic potential of a clinical isolate, CCUG 32053. This strain has a multipartite genome (4,632,079 bp) composed of a circular chromosome plus eight extrachromosomal replicons pYEE1-8: 3 chromids and 5 plasmids, with a total size of 1,247,173 bp. The genome has been significantly shaped by the acquisition of genomic islands, prophages ( and phage families) and numerous insertion sequences (ISs) representing seven IS families. Detailed comparative analysis with other complete genomic sequences of spp. (including FDAARGOS_252 and TT13, as well as non-pathogenic strains of other species in this genus) enabled us to identify species-specific genes and to predict putative determinants of virulence. This is the first attempt to identify pathoadaptive genetic information of and to estimate the role of the mobilome in the evolution of pathogenicity in this species.
PubMed: 30410477
DOI: 10.3389/fmicb.2018.02553 -
Genome Announcements Jan 2018TT13 was isolated from human skin because of its ability to degrade propylene glycol. Here, we present the whole-genome sequence of this strain; it possesses one...
TT13 was isolated from human skin because of its ability to degrade propylene glycol. Here, we present the whole-genome sequence of this strain; it possesses one 3.58-Mb chromosome and six plasmids. TT13 genome analysis indicated that this bacterium has denitrification potential.
PubMed: 29348361
DOI: 10.1128/genomeA.01514-17 -
Nefrologia : Publicacion Oficial de La... 2016
Topics: Animals; Catheter-Related Infections; Dogs; Environmental Exposure; Female; Gram-Negative Bacterial Infections; Humans; Middle Aged; Paracoccus; Peritoneal Dialysis; Peritonitis; Soil Microbiology
PubMed: 27039709
DOI: 10.1016/j.nefro.2016.02.009 -
Laboratory Animals Oct 2014Correct identification of bacteria is crucial for the management of rodent colonies. Some bacteria are difficult to identify phenotypically outside reference...
Correct identification of bacteria is crucial for the management of rodent colonies. Some bacteria are difficult to identify phenotypically outside reference laboratories. In this study, we evaluated the utility of 16S ribosomal DNA (rDNA) sequencing as a means of identifying a collection of 30 isolates of rodent origin which are conventionally difficult to identify. Sequence analysis of the first approximate 720 to 880 bp of the 5'- end of 16S rDNA identified 25 isolates (83.33%) with ≥ 99% similarity to a sequence of a type strain, whereas three isolates (10%) displayed a sequence similarity ≥ 97% but <99% to the type strain sequences. These similarity scores were used to define identification to species and genus levels, respectively. Two of the 30 isolates (6.67%) displayed a sequence similarity of ≥ 95 but <97% to the reference strains and were thus allocated to a family. This technique allowed us to document the association of mice with bacteria relevant for the colonies management such as Pasteurellaceae, Bordetella hinzii or Streptococcus danieliae. In addition, human potential pathogens such as Acinetobacter spp., Ochrobactrum anthropi and Paracoccus yeei or others not yet reported in mouse bacterial species such as Leucobacter chironomi, Neisseria perflava and Pantoea dispersa were observed. In conclusion, the sequence analysis of 16S rDNA proved to be a useful diagnostic tool, with higher performance characteristics than the classical phenotypic methods, for identification of laboratory animal bacteria. For the first time this method allowed us to document the association of certain bacterial species with the laboratory mouse.
Topics: Animal Husbandry; Animals; Animals, Laboratory; Bacteria; Mice; Molecular Sequence Data; RNA, Ribosomal, 16S; Rats; Sequence Analysis, DNA
PubMed: 24876090
DOI: 10.1177/0023677214538240 -
Eye & Contact Lens May 2014Paracoccus yeei is an environmental bacterium suspected to be responsible for ocular infections. In this study, culture-based and polymerase chain reaction (PCR)...
OBJECTIVES
Paracoccus yeei is an environmental bacterium suspected to be responsible for ocular infections. In this study, culture-based and polymerase chain reaction (PCR) sequencing-based investigations of a corneal scraping specimen confirmed P. yeei keratitis in one contact lens wearer.
METHODS
A 34-year-old male patient, who was a contact lens wearer, presented with a unilateral corneal ulcer. A corneal scraping was performed to conduct a microbiological investigation. Disease progress was favorable after a 5-week topical treatment, which combined rifampicin, ciprofloxacine, tobramycine, and dexamethasone. The final visual acuity of the right eye was 5/10 with correction, limited by a central corneal scar.
RESULTS
Culturing the corneal scraping specimen yielded P. yeei, which was also directly detected by PCR sequencing the bacterial 16S rDNA in the presence of negative controls. The P. yeei isolate was susceptible to ticarcillin, with a minimal inhibitory concentration (MIC) of <4 mg/L of ceftazidime (MIC of 0.5 mg/L), imipenem (MIC < 0.5 mg/L), gentamycin (MIC of 0.5 mg/L), tobramycin (MIC < 0.06 mg/L), rifampicin (MIC < 0.125 mg/L), ciprofloxacin (MIC < 0.5 mg/L), and colimycin (MIC < 2 mg/L). The isolate was resistant to fosfomycine with a MIC 32 > mg/L.
CONCLUSIONS
P. yeei is a new cause of keratitis that must be diagnosed by a laboratory analysis of corneal scrapings due to the potentially poor outcome of infection.
Topics: Adult; Anti-Bacterial Agents; Contact Lenses; Corneal Ulcer; Drug Therapy, Combination; Humans; Male; Paracoccus; Treatment Outcome
PubMed: 24045834
DOI: 10.1097/ICL.0b013e31829e8fc7 -
Medecine Et Maladies Infectieuses Jun 2013
Topics: Adenocarcinoma; Adrenal Cortex Hormones; Aged; Anti-Bacterial Agents; Antineoplastic Agents; Arthritis, Infectious; Arthritis, Rheumatoid; Ceftriaxone; Colonic Neoplasms; Diabetes Mellitus, Type 2; Female; Gram-Negative Bacterial Infections; Hip Joint; Humans; Immunocompromised Host; Immunosuppressive Agents; Paracoccus; RNA, Bacterial; RNA, Ribosomal, 16S; Ribotyping; Synovial Fluid
PubMed: 23751881
DOI: 10.1016/j.medmal.2013.04.004 -
Journal of Global Infectious Diseases Apr 2011Purine compounds are special types of alkaloids. Caffeine and aminophylline are considered the most important members of purines due to their wide use in therapeutics.
BACKGROUND
Purine compounds are special types of alkaloids. Caffeine and aminophylline are considered the most important members of purines due to their wide use in therapeutics.
AIMS
To detect any potential antibacterial effects on pathogenic bacteria of the widely prescribed members of purines caffeine and aminophylline.
MATERIALS AND METHODS
Two species of gram-positive bacteria and five species of gram-negative bacteria were exposed to these purine agents. Antibacterial effects of the tested purines were determined using the spectrophotometer method to assess the minimum inhibition concentrations (MIC).
RESULTS
Among the strains of bacteria tested, Bacillus subtilis showed the most susceptibility to purine agents. Staphylococcus aureus and Bacillus subtilis were found to be more susceptible to caffeine than the other strains. Aminophylline showed inhibitory action on many isolates, especially at the concentration of 10mg/ml. Paracoccus yeei demonstrated resistance to all tested purine compounds up to a concentration of 10.5mg/ml.
CONCLUSIONS
Caffeine and aminophylline had the ability to inhibit many strains of pathogenic bacteria.
PubMed: 21731299
DOI: 10.4103/0974-777X.81689