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Transplant Infectious Disease : An... Apr 2011After a successful cardiac transplantation, routine endomyocardial biopsies showed severe infiltrates comparable with myocarditis. Polymerase chain reaction analysis of...
After a successful cardiac transplantation, routine endomyocardial biopsies showed severe infiltrates comparable with myocarditis. Polymerase chain reaction analysis of native myocardial samples revealed infection with Paracoccus yeei, and the clinical condition of the patient deteriorated. After administration of ciprofloxacin, his clinical condition improved, and further biopsies showed no infiltrates in the cardiac specimens. To our knowledge this is the first documented case of P. yeei infection in a heart transplant patient.
Topics: Adult; Anti-Bacterial Agents; Clindamycin; Gram-Negative Bacterial Infections; Heart Transplantation; Humans; Male; Paracoccus
PubMed: 20854281
DOI: 10.1111/j.1399-3062.2010.00571.x -
Journal of Global Infectious Diseases May 2010Ibuprofen and acetaminophen are common chemical agents that have anti-inflammatory, antipyretic, and analgesic activity.
BACKGROUND
Ibuprofen and acetaminophen are common chemical agents that have anti-inflammatory, antipyretic, and analgesic activity.
AIMS
To detect any potential antibacterial effects of ibuprofen and acetaminophen on pathogenic bacteria.
MATERIALS AND METHODS
Ibuprofen and acetaminophen were tested for antibacterial activity against seven isolates of bacteria including gram positive bacteria (Staphylococci aureus and Bacillus subtilis) and gram negative bacteria (E. coli, Enterobacter aerogenes, Enterobacter cloacae, Salmonella typhi and Paracoccus yeei). Spectrophotometer assay was applied to determine the antibacterial activities of ibuprofen and acetaminophen. Three controls were included in this study: Ampicilline sodium (20 mug/ml); cefotaxime sodium (20 mug/ml) and chemical free medium.
RESULTS
Staphylococcus aureus and Paracoccus yeei were susceptible to lower concentrations of ibuprofen and acetaminophen (MIC=1.25 mg/ml), while two strains of Enterobacter exhibited resistance to these agents.
CONCLUSIONS
Ibuprofen and acetaminophen showed a potential antibacterial effect on isolated strains of bacteria. They had the same ability to inhibit bacterial growth.
PubMed: 20606962
DOI: 10.4103/0974-777X.62880 -
Journal of Clinical Microbiology Jan 2010A 68-year-old man who had undergone two penetrating keratoplasties of his left eye was admitted with early corneal graft failure. Culture of the anterior chamber fluid...
A 68-year-old man who had undergone two penetrating keratoplasties of his left eye was admitted with early corneal graft failure. Culture of the anterior chamber fluid yielded Paracoccus yeei, a nonfermentative gram-negative bacillus which thus far had only been implicated in ocular disease by means of PCR and 16S rRNA gene sequencing directly on patient material.
Topics: Aged; Bacterial Typing Techniques; DNA, Bacterial; DNA, Ribosomal; Graft Rejection; Gram-Negative Bacterial Infections; Humans; Keratitis; Keratoplasty, Penetrating; Male; Molecular Sequence Data; Paracoccus; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 19889897
DOI: 10.1128/JCM.01798-09 -
International Journal of Infectious... Feb 2010Paracoccus yeei was identified as the etiologic agent of peritonitis in an ambulatory peritoneal dialysis patient. While the old biochemical identification kits are not...
Paracoccus yeei was identified as the etiologic agent of peritonitis in an ambulatory peritoneal dialysis patient. While the old biochemical identification kits are not able to identify this species, the new colorimetric VITEK 2 GN card correctly identified this isolate in 7hours. Its identity was confirmed by sequencing of the 16S rRNA gene.
Topics: Adult; Ambulatory Care; Bacterial Typing Techniques; Genes, rRNA; Gram-Negative Bacterial Infections; Humans; Male; Opportunistic Infections; Paracoccus; Peritoneal Dialysis; Peritonitis; RNA, Ribosomal, 16S; Reagent Kits, Diagnostic; Sequence Analysis, DNA
PubMed: 19556157
DOI: 10.1016/j.ijid.2009.03.030 -
Medicine May 2008The etiologic evaluation of uveitis is frequently unsuccessful when noninvasive methods are used. We conducted a prospective study to evaluate systematic screening for...
The etiologic evaluation of uveitis is frequently unsuccessful when noninvasive methods are used. We conducted a prospective study to evaluate systematic screening for pathogens of uveitis. All patients with uveitis referred to the participating tertiary ophthalmology departments from January 2001 to September 2007 underwent intraocular and serum specimen collection. The standardized protocol for laboratory investigations included universal polymerase chain reaction (PCR)-based detection of any bacteria and mycoses, specific PCR-based detection of fastidious (difficult-to-grow) bacteria and herpes viruses, and culture of vitreous fluid. Sera were tested for fastidious bacteria. Among the 1321 included patients (1520 specimens), infection was diagnosed in 147 (11.1%) patients: 78 (53%) were caused by fastidious bacteria that included spirochetes, Bartonella species, intracellular bacteria (Chlamydia species, Rickettsia species, Coxiella burnetii), and Tropheryma whipplei; 18 by herpes viruses; and 9 by fungi. Bartonella quintana, Coxiella burnetii, Paracoccus yeei, Aspergillus oryzae, and Cryptococcus albidus were found to be associated with uveitis for the first time, to our knowledge. We recommend applying a 1-step diagnostic procedure that incorporates intraocular, specific microbial PCR with serum analyses in tertiary centers to determine the etiology of uveitis.
Topics: Adult; Aged; Aged, 80 and over; Bacteria; Child, Preschool; Eye Infections, Bacterial; Female; Humans; Male; Middle Aged; Polymerase Chain Reaction; Serologic Tests; Tropheryma; Uveitis
PubMed: 18520326
DOI: 10.1097/MD.0b013e31817b0747 -
Journal of Clinical Microbiology Jul 2004Paracoccus yeei was isolated in pure culture from an aerobic blood culture and bulla fluid from a 67-year-old male. The biochemical identification scheme for this...
Paracoccus yeei was isolated in pure culture from an aerobic blood culture and bulla fluid from a 67-year-old male. The biochemical identification scheme for this recently described species is outlined. Because of its reaction pattern it is not unlikely that P. yeei is underdiagnosed.
Topics: Aged; Base Sequence; Gram-Negative Bacterial Infections; Humans; Male; Molecular Sequence Data; Paracoccus
PubMed: 15243119
DOI: 10.1128/JCM.42.7.3366-3368.2004