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Veterinary Medicine and Science Jan 2023As the clinical outcome of bite-associated infection is related to the oral commensals, evaluating their composition and antibiotic susceptibility pattern can provide...
BACKGROUND
As the clinical outcome of bite-associated infection is related to the oral commensals, evaluating their composition and antibiotic susceptibility pattern can provide more information for the antibiotic treatment of wound infections and increase the awareness of the multidrug-resistant bacteria in cat oral flora.
AIMS
This study was conducted to identify the various bacterial species in the oral cavity of cats. It aimed to identify the composition of cat oral flora and antibiotic resistant bacterial stains.
MATERIALS AND METHODS
Twenty-two cats were sampled for bacterial evaluation. Matrix-assisted laser desorption/ionization time of flight mass spectrometry was used to provide rapid and reliable detection and identification of the bacterial species. Antibiotic susceptibility tests were performed in the identified isolates to determine the antibiotic susceptibility pattern and to detect the multidrug-resistant bacteria in the cat oral cavities.
RESULTS
A total of 54 isolates were identified, Pasteurella was the genus most commonly isolated from the oral cavity of cats (19/54, 35.19%), followed by Neisseria spp. (8/54, 14.81%) and Staphylococcus spp. (7/54, 12.96%). Uncommon oral flora were isolated from the samples, including Pasteurella canis, Inquilinus limosus and the Enterobacteriaceae family of Escherichia coli, Klebsiella pneumoniae and Serratia marcescens. Fourteen different multidrug-resistant bacteria were detected, including Pasteurella species (4/14), Bacillus species (2/14), Neisseria species (3/14), Escherichia species (1/14) and Staphylococcus species (4/14).
DISCUSSION AND CONCLUSIONS
This study's findings will increase the understanding of the composition of cat oral flora in Hong Kong, which can provide more evidence-based information for the prophylactic treatment of patients with cat bite infections. Moreover, the study identified and detected the antibiotic resistance pattern and multidrug-resistant bacteria in the cat oral cavity, which can help cat owners increase their awareness of maintaining regular oral hygiene for their cats to prevent the spread of pathogens from cats to humans.
Topics: Animals; Cats; Humans; Anti-Bacterial Agents; Hong Kong; Enterobacteriaceae; Drug Resistance, Multiple, Bacterial; Escherichia coli
PubMed: 36520663
DOI: 10.1002/vms3.1040 -
Microbiology Resource Announcements Jul 2022We report the draft genome sequence of Pasteurella canis strain PA42, which was isolated from the blood of a diseased dog in Japan in 2021. The 2.151-Mbp genome has a...
We report the draft genome sequence of Pasteurella canis strain PA42, which was isolated from the blood of a diseased dog in Japan in 2021. The 2.151-Mbp genome has a G+C content of 36.6%. Sequences unmapped to the reference genome sequence of NCTC 11621 (GenBank accession number UGTV00000000.1) were characterized.
PubMed: 35638811
DOI: 10.1128/mra.00260-22 -
Microorganisms Apr 2022In two previous surveys, the U.S. Food and Drug Administration (FDA) identified microbial contamination in 53 of 112 (47%) unopened tattoo inks and tattoo-ink-related...
In two previous surveys, the U.S. Food and Drug Administration (FDA) identified microbial contamination in 53 of 112 (47%) unopened tattoo inks and tattoo-ink-related products (e.g., diluents) from 15 manufacturers in the U.S. In this study, we primarily focused our microbiological survey on permanent makeup (PMU) inks. We conducted a survey of 47 unopened PMU inks from nine manufacturers and a comparative species-centric co-occurrence network (SCN) analysis using the survey results. Aerobic plate count and enrichment culture methods using the FDA's (BAM) Chapter 23 revealed that 9 (19%) inks out of 47, from five manufacturers, were contaminated with microorganisms. The level of microbial contamination was less than 250 CFU/g in eight inks and 980 CFU/g in one ink. We identified 26 bacteria that belong to nine genera and 21 species, including some clinically relevant species, such as , , , and . Among the identified microorganisms, the SCN analysis revealed dominance and a strong co-occurrence relation of spore-forming extreme environment survivors, spp., with close phylogenetic/phenotypic relationships. These results provide practical insights into the possible microbial contamination factors and positive selection pressure of PMU inks.
PubMed: 35456870
DOI: 10.3390/microorganisms10040820 -
Cureus Nov 2021is a Gram-negative coccobacilli from the family. The most common form of transmission to humans is a bite from a dog or a cat. We report a case of a 90-year-old woman...
is a Gram-negative coccobacilli from the family. The most common form of transmission to humans is a bite from a dog or a cat. We report a case of a 90-year-old woman who presented with septic arthritis in the right knee and bacteremia two weeks after a cat bite. The patient was treated with arthrocentesis and directed antimicrobial therapy. Human infection is a rare occurrence, making this a case of note.
PubMed: 34912619
DOI: 10.7759/cureus.19478 -
Forensic Science International. Genetics Sep 2021Analyzing ambiguous bite marks using conventional morphological approaches to identify attackers is difficult; thus, applying molecular biological methods for...
Analyzing ambiguous bite marks using conventional morphological approaches to identify attackers is difficult; thus, applying molecular biological methods for identifying an attacker from their saliva is a possible approach in a forensic investigation. This study aimed to establish oral bacterial DNA-based human and canine saliva markers and develop a practical method for their discrimination. We considered Streptococcus oralis and Pasteurella canis as human and canine saliva marker candidates, respectively. Duplex bacterial DNA detection using melting curve analysis was designed and evaluated for forensic applicability using proof-of-concept experiments. S. oralis DNA was detected from human saliva samples from 30 out of 30 individuals, and P. canis DNA was detected from canine saliva samples from 73 out of 77 individuals (26 dog breeds). Additionally, both bacterial DNA markers were accurately detected from human blood-contaminated saliva samples and mock indistinct bite marks. Our results indicate that both bacterial DNA markers were sensitive, robust, and discriminating saliva markers. We consider that our duplex bacterial DNA examination is a simple, practical, and useful method for the detection of saliva from indistinct bite marks and discrimination between human and canine saliva.
Topics: Animals; DNA, Bacterial; Dogs; Genetic Markers; Humans; Pasteurella; Saliva
PubMed: 34332321
DOI: 10.1016/j.fsigen.2021.102566 -
[Investigation of Skin Adherent Bacterial Flora in Dogs by MALDI-TOF MS and 16S rRNA Gene Analysis].Rinsho Biseibutsu Jinsoku Shindan... Dec 2020Due to the increase in the number of companion animal breeders in Japan, there are more opportunities for companion animals to come into contact with humans than before....
Due to the increase in the number of companion animal breeders in Japan, there are more opportunities for companion animals to come into contact with humans than before. Therefore, we investigated the bacterial flora adhering to the skin of dogs and the bacterial flora was analyzed for the presence of zoonotic bacteria that infect humans from companion animals. With the cooperation of students enrolled in the Department of Medical Technology and Science, Faculty of Fukuoka Health Care, International University of Health and Welfare. 39 samples were collected from the abdomen, back and paws of 13 healthy dogs using sterile swabs by the scraping method. The isolation culture was carried out only for facultative anaerobic bacteria to obligate aerobic bacteria and Bacterial identification was determined by MALDI-TOF MS and 16S rRNA gene analysis. Among the identified strains were , , , which were difficult to detect in humans. The overall ratio of detected bacteria was 35% for coagulasenegative staphylococci, 14% for coagulase-positive staphylococci, 5% for Enterobacteriaceae, and 45% for natural environment. In the future, it is expected that extended-spectrum β-lactamase producing bacteria and drug-resistant bacteria such as Carbapenem-resistant will also be transmitted to humans through contact with companion animals.
Topics: Animals; Dogs; Japan; Pasteurella; RNA, Ribosomal, 16S; Skin; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Staphylococcus; Staphylococcus intermedius
PubMed: 33478234
DOI: No ID Found -
Acta Veterinaria Hungarica Sep 2020The occurrence of members of the Pasteurellaceae and Neisseriaceae families was studied in dogs and cats. A total of 110 nasal and pharyngeal swab samples from 47 dogs...
The occurrence of members of the Pasteurellaceae and Neisseriaceae families was studied in dogs and cats. A total of 110 nasal and pharyngeal swab samples from 47 dogs and 8 cats were collected. Most of the strains were identified by 16S rDNA sequencing, except Frederiksenia canicola and Pasteurella multocida where species-specific polymerase chain reactions were applied. The most frequently isolated species was F. canicola, which occurred only in dogs, mainly in the pharyngeal cavity. The second commonest bacterium, P. multocida was found in both types of samples and in both hosts. Other species from the family Pasteurellaceae, such as Haemophilus haemoglobinophilus, Pasteurella canis and P. dagmatis, were detected only in dogs. All isolated species belonging to the family Neisseriaceae, mainly representing Neisseria weaveri, were found only in the pharyngeal cavity. Neisseria weaveri and N. zoodegmatis could be detected in both hosts. Neisseria dumasiana and N. canis were isolated from dogs, while N. shayeganii only from a cat. For phylogenetic analysis, rpoB gene sequencing was performed, where the strains were on monophyletic branches and clearly separated from each other. In this study, recently described species such as F. canicola, N. shayeganii and N. dumasiana were detected that had never been isolated in Hungary before.
Topics: Animals; Cat Diseases; Cats; Dog Diseases; Dogs; Gram-Negative Bacterial Infections; Hungary; Incidence; Microbiota; Neisseriaceae; Pasteurellaceae; Pasteurellaceae Infections; Pharynx; Respiratory System; Respiratory Tract Diseases
PubMed: 33141116
DOI: 10.1556/004.2020.00036 -
JPRAS Open Sep 2019Zoonotic infections represent an uncommon phenomenon. Few people with pets realise the infectious risk this entails. This case describes a infection of a breast implant...
Zoonotic infections represent an uncommon phenomenon. Few people with pets realise the infectious risk this entails. This case describes a infection of a breast implant following close contact between a patient and her cat. A 59-year-old woman developed infection of her breast implant 7 months after implantation. Surgical revision was performed after failure of conservative treatment with antibiotics. Perioperative samples from the periprosthetic fluid were positive for , a Gram-negative coccobacillus that is present in the oropharyngeal commensal flora of cats and dogs. History revealed that the patient owned a cat for 2 years. This case highlights the possible risk of zoonotic infections in humans with protheses following close contact with a cat. Antibiotic therapy and surgical revision, with or without removal of the prosthesis constitute the cornerstone of treatment in such cases.
PubMed: 32158890
DOI: 10.1016/j.jpra.2019.07.006 -
Journal of Clinical Laboratory Analysis Jul 2020
Topics: Aged; Animals; Bites and Stings; Dogs; Drug Resistance, Bacterial; Genome, Bacterial; Genome-Wide Association Study; Humans; Male; Osteomyelitis; Pasteurella; Pasteurella Infections
PubMed: 32118316
DOI: 10.1002/jcla.23274 -
Polish Archives of Internal Medicine Apr 2020
Topics: Animals; Communicable Diseases; Equidae; Horses; Humans; Leukemia, Myeloid, Acute; Pasteurella
PubMed: 31933489
DOI: 10.20452/pamw.15142