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American Journal of Physiology. Heart... Mar 2024Pentoxifylline is a nonselective phosphodiesterase inhibitor used for the treatment of peripheral artery disease. Pentoxifylline acts through cyclic adenosine... (Randomized Controlled Trial)
Randomized Controlled Trial
Pentoxifylline is a nonselective phosphodiesterase inhibitor used for the treatment of peripheral artery disease. Pentoxifylline acts through cyclic adenosine monophosphate, thereby enhancing red blood cell deformability, causing vasodilation and decreasing inflammation, and potentially stimulating ventilation. We conducted a double-blind, placebo-controlled, crossover, counter-balanced study to test the hypothesis that pentoxifylline could lower blood viscosity, enhance cerebral blood flow, and decrease pulmonary artery pressure in lowlanders following 11-14 days at 3,800 m. Participants (6 males/10 females; age, 27 ± 4 yr old) received either a placebo or 400 mg of pentoxifylline orally the night before and again 2 h before testing. We assessed arterial blood gases, venous hemorheology (blood viscosity, red blood cell deformability, and aggregation), and inflammation (TNF-α) in room air (end-tidal oxygen partial pressure, ∼52 mmHg). Global cerebral blood flow (gCBF), ventilation, and pulmonary artery systolic pressure (PASP) were measured in room air and again after 8-10 min of isocapnic hypoxia (end-tidal oxygen partial pressure, 40 mmHg). Pentoxifylline did not alter arterial blood gases, TNF-α, or hemorheology compared with placebo. Pentoxifylline did not affect gCBF or ventilation during room air or isocapnic hypoxia compared with placebo. However, in females, PASP was reduced with pentoxifylline during room air (placebo, 19 ± 3; pentoxifylline, 16 ± 3 mmHg; = 0.021) and isocapnic hypoxia (placebo, 22 ± 5; pentoxifylline, 20 ± 4 mmHg; = 0.029), but not in males. Acute pentoxifylline administration in lowlanders at 3,800 m had no impact on arterial blood gases, hemorheology, inflammation, gCBF, or ventilation. Unexpectedly, however, pentoxifylline reduced PASP in female participants, indicating a potential effect of sex on the pulmonary vascular responses to pentoxifylline. We conducted a double-blind, placebo-controlled study on the rheological, cardiorespiratory and cerebrovascular effects of acute pentoxifylline in healthy lowlanders after 11-14 days at 3,800 m. Although red blood cell deformability was reduced and blood viscosity increased compared with low altitude, acute pentoxifylline administration had no impact on arterial blood gases, hemorheology, inflammation, cerebral blood flow, or ventilation. Pentoxifylline decreased pulmonary artery systolic pressure in female, but not male, participants.
Topics: Male; Humans; Female; Young Adult; Adult; Pentoxifylline; Hemorheology; Tumor Necrosis Factor-alpha; Hypoxia; Oxygen; Acclimatization; Inflammation; Gases; Cerebrovascular Circulation; Altitude
PubMed: 38241007
DOI: 10.1152/ajpheart.00783.2023 -
Scientific Reports Jan 2024The study investigates the flow of a Newtonian Cu O nanofluid through a non-Darcy porous medium with radially varying viscosity, which is crucial for various industries...
The study investigates the flow of a Newtonian Cu O nanofluid through a non-Darcy porous medium with radially varying viscosity, which is crucial for various industries such as pharmaceuticals, chemicals, nuclear, solar, and solar technologies. The peristaltic motion of the nanofluid is studied with thermal radiation and chemical reaction effects, and the viscosity varies with both radius and axial coordinates. The study assumes low Reynolds and long wavelength assumptions and uses the homotopy perturbation technique to obtain a semi-analytical solution of velocity, temperature, nanoparticle concentration, and skin friction. The results show that axial velocity increases with the increase of slip velocity and viscosity parameters, while wave amplitude and chemical reaction parameters increase while nanoparticle concentration decreases. High viscosity parameters allow fluid nanoparticles to gain more active energy and move more freely, which is the main idea behind crude oil refinement. This physical modeling is essential for physiological flows, such as stomach juice flow during endoscope insertion.
PubMed: 38238338
DOI: 10.1038/s41598-023-48692-3 -
Scientific Reports Jan 2024Early diagnosis and intervention of Alzheimer's disease (AD) are particularly important to delay the pathological progression. Although fluorescent probes have been...
Early diagnosis and intervention of Alzheimer's disease (AD) are particularly important to delay the pathological progression. Although fluorescent probes have been widely employed for investigating and diagnosing AD, their biological applications are significantly restricted due to the low penetration ability of the blood-brain barrier (BBB) in vivo. In this study, we reported the first Golgi-targeted two-photon (TP) fluorescent probe, DCM-DH, for detecting viscosity in the Golgi apparatus. The probe was rationally designed to exhibit superior analytical performance including high sensitivity, specific Golgi-targeting, efficient BBB penetration ability, and deep tissue penetration (247 μm) in the brains of AD model mice. Using the probe, we demonstrated that the fluorescence intensity in the human liver cancer cell (HepG2 cells) was higher than that of human normal liver cell (LO2 cells), and the brain viscosity of AD model mice increased significantly. We anticipate that this competent tool could be easily extended to other AD biomarkers for fundamental research on this detrimental disease.
Topics: Mice; Humans; Animals; Alzheimer Disease; Viscosity; Early Detection of Cancer; Brain; Blood-Brain Barrier; Fluorescent Dyes; Amyloid beta-Peptides
PubMed: 38228652
DOI: 10.1038/s41598-023-50789-8 -
PloS One 2024This study aims to compare two surgical techniques, the standard Vein-to-Artery and the newer Artery-to-Vein (Radial Artery Deviation And Reimplantation; RADAR), for...
Comparative analysis of RADAR vs. conventional techniques for AVF maturation in patients with blood viscosity and vessel elasticity-related diseases through fluid-structure interaction modeling: Anemia, hypertension, and diabetes.
PURPOSE
This study aims to compare two surgical techniques, the standard Vein-to-Artery and the newer Artery-to-Vein (Radial Artery Deviation And Reimplantation; RADAR), for enhancing the success of Arterio-Venous Fistula maturation in end-stage renal disease patients. The impact of diseases like anemia, diabetes, hypertension, and chronic kidney disease were considered. The goals are to advance Arterio-Venous Fistula (AVF) surgery, improve patient outcomes, and contribute to evidence-based surgical guidelines.
METHODS
Fluid-structure interaction modeling was employed to investigate how hemodynamic and mechanical stresses impact arteriovenous fistula maturation, with a particular focus on the role of wall shear stress in determining maturation outcomes. The critical threshold for vessel injury was identified as wall shear stress values exceeding 35 N/m2, while stenosis formation was projected to occur at levels below 1 N/m2. This work introduced a novel approach by considering disease-related factors, including blood viscosity (anemia), and vessel elasticity (diabetes, hypertension, and chronic kidney diseases), which directly influence hemodynamics and the generation of wall shear stress. Furthermore, the model was designed to incorporate varying thicknesses and elasticities for both the vein and artery, accurately representing authentic vascular anatomy.
RESULTS
The RADAR technique has demonstrated superior performance compared to the standard technique by providing appropriate wall shear stress in critical regions and minimizing the risk of wall damage. Its use of a thicker vessel also reduces the risk of vessel injury, making it particularly effective for patients with Chronic Kidney Disease (CKD), hypertension, anemia, and diabetes, ensuring optimal blood flow and fewer complications. However, there are minor concerns about stenosis formation in hypertension and anemia cases, which could be mitigated by adjusting the anastomosis angle to be lower than 30°.
CONCLUSION
Diabetes and hypertension have significant physiological effects that increase the risks associated with arteriovenous fistula maturation. The anemic condition resulting from CKD may help reduce vessel injury but raises concerns about potential stenosis formation. Despite these co-morbidities, the RADAR technique has demonstrated its ability to induce more favorable hemodynamic changes, promoting arteriovenous fistula maturation.
Topics: Humans; Blood Viscosity; Arteriovenous Shunt, Surgical; Constriction, Pathologic; Kidney Failure, Chronic; Diabetes Mellitus; Hypertension; Arteriovenous Fistula; Anemia; Renal Dialysis
PubMed: 38227602
DOI: 10.1371/journal.pone.0296631 -
Archives Animal Breeding 2023This study aimed to show the effect of bile acid (BA) and xylanase (Xyl) supplementation on the growth, fat digestibility, serum lipid metabolites, and ileal digesta...
This study aimed to show the effect of bile acid (BA) and xylanase (Xyl) supplementation on the growth, fat digestibility, serum lipid metabolites, and ileal digesta viscosity of broilers. A total of 720 1 d old male broilers were allocated to one of nine treatments with four replicates in each under a factorial design arrangement of three levels of BA (0 %, 0.25 %, and 0.50 %) and three levels of Xyl (0 %, 0.05 %, and 0.10 %) supplementation. The duration of the experiment was 35 d (7-42 d). Growth performance, blood lipids, fat digestibility, and ileal digesta viscosity were determined. The experimental treatments did not affect feed intake (FI) and weight gain (WG). Supplementation of BA or Xyl did not significantly ameliorate the feed conversion rate (FCR) (). The addition of BA linearly increased fat digestibility. At 7-21 d of age, the addition of BA or Xyl had a significant () increase in serum cholesterol (Chol) but no significant difference for other serum lipid parameters in broiler chickens fed with Xyl in the starter and grower periods. However, the supplementation of 0.5 % BA at 7-21 d of age significantly increased the Chol and low-density-lipoprotein (LDL) levels. The results of this trial revealed that the supplementation of xylanases had a great effect on the degradation of arabinoxylan from wheat, which led to a relatively greater reduction in ileal digesta viscosity; it was also found that supplementation of BA significantly increased the concentration of serum lipid metabolites, whereas BA and Xyl supplementation linearly increased the fat digestibility of the birds fed wheat and tallow diets.
PubMed: 38205380
DOI: 10.5194/aab-66-451-2023 -
Heliyon Jan 2024Hypertension has become a part of the lives of many people worldwide. With the development, an increasing number of people have begun to control their hypertension...
Antihypertensive effect and mechanism of the traditional recipe of medicine food homology (Buyang Huanwu Decoction) in China: Meta analysis and network pharmacological exploration.
BACKGROUND
Hypertension has become a part of the lives of many people worldwide. With the development, an increasing number of people have begun to control their hypertension through products of medicine food homology, such as Buyang Huanwu Decoction (BYHWD). However, there has been no objective review of the regulation of hypertension by BYHWD.
METHODS
As of 9 October 2023, this review made a detailed search of nine databases to look for random controlled trials (RCTs) focused on the use of BYHWD for treating hypertension. This was followed by network pharmacological analysis, and molecular docking assessment using AutoDockTools to explore the mode of action.
RESULTS
BYHWD was effective in reducing SBP (MD: 0.767; 95 % CI: 0.629, 0.905; = 0.000), DBP (MD: 0.427; 95 % CI: 0.292, 0.561; = 0.000), 24h SBP (MD: 0.665; 95 % CI: 0.368, 0.962; = 0.000), 24h DBP (MD: 0.547; 95 % CI: 0.318, 0.777; = 0.000), dSBP (MD: 0.625; 95 % CI: 0.395, 0.855; = 0.000), dDBP (MD: 0.632; 95 % CI: 0.401, 0.862; = 0.000), nSBP (MD: 0.859; 95 % CI: 0.340, 1.377; = 0.001), nDBP (MD: 0.704; 95 % CI: 0.297, 1.112; = 0.001), pv (MD: 1.311; 95 % CI: 0.363, 2.259; = 0.007) and NIHSS (MD: 1.149; 95 % CI: 0.100, 2.199; = 0.032), and elevating CER (OR = 2.848; 95 % CI: 1.388, 5.843; = 0.004). However, BYHWD did not significantly reduce HCY, and there was no significant difference in the incidence of AE. In terms of the mechanism of action, the main active ingredient of BYHWD is quercetin, and the core targets are AKT1, MMP9, and others. Molecular docking also showed that quercetin mainly interacts with the amino acid residue CYS-28 of MMP2. Second, the KEGG analysis showed that BYHWD mainly act on HIF-1, Apelin, and cGMP-PKG signalling pathways, and GO analysis showed that it related to the apical part of the cell, circulatory system processes, and nuclear receptor activity. BYHWD can lowered blood pressure, reduced plasma viscosity, and restored neurological function with good tolerability, and had no significant effect on HCY levels. This study further demonstrated that quercetin is the main active ingredient of BYHWD that acts via the AKT1 and HIF-1 signalling pathways. These results provide new guidance for people's dietary choices by the general public.
PubMed: 38205319
DOI: 10.1016/j.heliyon.2023.e23474 -
Clinical and Applied... 2024The pathogenesis of venous thromboembolism in multiple myeloma is still poorly understood because multiple factors are involved. In particular, the increase in whole... (Observational Study)
Observational Study
The pathogenesis of venous thromboembolism in multiple myeloma is still poorly understood because multiple factors are involved. In particular, the increase in whole blood viscosity has a key role and, therefore, we performed an evaluation of some hemorheological determinants in multiple myeloma patients, putting them in relation to the thrombotic risk, with the aim to evaluate if an alteration of the hemorheological pattern was associated with a higher thrombotic risk. We performed an observational retrospective cohort study with data collected from January 2017 to September 2022. In a group of 190 patients with newly diagnosed multiple myeloma, we have examined the trend of calculated blood viscosity according to the Merrill formula, and we stratified the patients for the thrombotic risk in accordance with the IMWG/NCCN guidelines and with IMPEDE VTE score. Using the thrombotic risk stratification proposed by IMWG/NCCN any variation in calculated blood viscosity is evident, while, with the IMPEDE VTE score, we observed an increase in calculated blood viscosity in patients with "intermediate + high" risk. The calculated blood viscosity is higher in subjects presenting an "intermediate + high" thrombotic risk according to the IMPEDE VTE score. This association could therefore lay the groundwork for further research with the aim to confirm the role of hemorheological pattern in MM-related thrombotic risk.
Topics: Humans; Blood Viscosity; Multiple Myeloma; Retrospective Studies; Risk Factors; Thrombosis; Venous Thromboembolism
PubMed: 38173275
DOI: 10.1177/10760296231222477 -
Computer Methods and Programs in... Feb 2024This study aims to assess the dynamic impact of non-Newtonian cerebral arterial circulation on electrical conductivity within a realistic multi-compartment head model....
BACKGROUND AND OBJECTIVE
This study aims to assess the dynamic impact of non-Newtonian cerebral arterial circulation on electrical conductivity within a realistic multi-compartment head model. Evaluating this research question is crucial and challenging due to its relevance to electrophysiological modalities like transcranial electrical stimulation (tES), electro-/magnetoencephalography (EEG/MEG), and electrical impedance tomography (EIT). In these modalities, accurate forward modeling depends on the electrical conductivity, which is affected by complex tortuous vessel networks, limited data acquisition in Magnetic Resonance Imaging (MRI), and non-linear blood flow phenomena, including shear rate and viscosity in non-Newtonian fluid.
METHODS
To obtain an approximation for the blood concentration, we first use Navier-Stokes equations (NSEs) to solve for the pressure and velocity of the blood in the major vessels. Then Fick's law is used to solve for the blood concentration in the tissues. Finally, Archie's law is used to estimate the electrical conductivity distribution based on the blood concentration.
RESULTS
The results, obtained with an open 7 Tesla MRI dataset, suggest that a dynamic model of cerebral blood flow (CBF) for both arterial and microcirculation can be established; we find blood pressure and electrical conductivity distributions given a numerically simulated pulse sequence and approximate the blood concentration and electrical conductivity inside the brain based on those.
CONCLUSIONS
Our model provides an approximation of the dynamical blood flow and the corresponding electrical conductivity distribution in the different parts of the brain. The advantage of our approach is that it is applicable with limited a priori information about the blood flow and with an arbitrary head model distinguishing the arteries.
Topics: Arteries; Hemodynamics; Magnetoencephalography; Cerebrovascular Circulation; Electric Conductivity
PubMed: 38157828
DOI: 10.1016/j.cmpb.2023.107983 -
International Journal of Molecular... Dec 2023Serotonin and interleukin 10 (IL-10) may play a role in gestational diabetes mellitus. Hyperglycemic environment, the detrusor musculature of the bladder and pelvic...
Serotonin and interleukin 10 (IL-10) may play a role in gestational diabetes mellitus. Hyperglycemic environment, the detrusor musculature of the bladder and pelvic floor muscles may become damaged, leading to urination problems and urine viscosity in pregnant women with gestational diabetes mellitus and pregnancy-specific urinary incontinence. Urine and blood samples were collected from pregnant women between 24 and 28 weeks of gestation. The serotonin concentration and cytokine IL-10 levels were evaluated in plasma and urine. In the total blood and urine, the viscosity was evaluated in the presence and absence of exogenous serotonin and IL-10. The plasma serotonin levels decreased, while the urine serotonin levels increased in the normoglycemic incontinent (NG-I), hyperglycemic continent (GDM-C), and hyperglycemic incontinent (GDM-I) groups. The IL-10 in the plasma decreased in the GDM-I group and was higher in the urine in the NG-I and GDM-I groups. The blood viscosity was higher, independently of urinary incontinence, in the GDM groups. The serotonin increased the blood viscosity from women with GDM-C and urine in the NG-I, GDM-C, and GDM-I groups. Blood and urine in the presence of IL-10 showed a similar viscosity in all groups studied. Also, no difference was observed in the viscosity in either the blood or urine when in the presence of serotonin and IL-10. These findings suggest that serotonin and IL-10 have the potential to reduce blood viscosity in pregnant women with gestational diabetes and specific urinary incontinence, maintaining values similar to those in normoglycemic women's blood.
Topics: Pregnancy; Female; Humans; Diabetes, Gestational; Interleukin-10; Serotonin; Viscosity; Urinary Incontinence
PubMed: 38138954
DOI: 10.3390/ijms242417125 -
Foods (Basel, Switzerland) Dec 2023The aim of this study was to compare the properties of isolated starches from ten commonly consumed rice varieties in China and to investigate their possible...
The aim of this study was to compare the properties of isolated starches from ten commonly consumed rice varieties in China and to investigate their possible association. In addition, principal component analysis (PCA) and correlation analysis were performed to demonstrate the weight or relevance of different properties. The starch granules had an irregular polyhedral structure. The crystalline structure had an orthogonal arrangement, which is characteristic of A-type starch with nanocrystals with an orthorhombic crystal structure. In addition, higher levels of rapidly digestible starch (72.43 to 74.32%) and resistant starch (2.27 to 2.3%) were found in glutinous rice starch. The highest content of slowly digestible starch (59.48%) was found in starch isolated from black rice, which may be an ideal rice variety for controlling blood glucose and weight. Starch isolated from red Hani terrace rice showed the highest thermal stability during cooking and the highest resistance to a high shear force treatment. In addition, the PCA suggests that the amylose content of starch largely determines the functional properties of starch and positively correlates with the peak viscosity and setback viscosity of the starch pasting. The results of this study will enrich the scientific knowledge of various rice starches and promote their application in the food industry and other industries.
PubMed: 38137295
DOI: 10.3390/foods12244492