-
GeoHealth Apr 2024The Multi-Threat Medical Countermeasure (MTMC) technique is crucial for developing common biochemical signaling pathways, molecular mediators, and cellular processes....
The Multi-Threat Medical Countermeasure (MTMC) technique is crucial for developing common biochemical signaling pathways, molecular mediators, and cellular processes. This study revealed that the Nod-like receptor 3 (NLRP3) inflammasome pathway may be a significant contributor to the cytotoxicity induced by various organophosphorus pesticides (OPPs). The study demonstrated that exposure to six different types of OPPs (paraoxon, dichlorvos, fenthion, dipterex, dibrom, and dimethoate) led to significant cytotoxicity in BV2 cells, which was accompanied by increased expression of NLRP3 inflammasome complexes (NLRP3, ASC, Caspase-1) and downstream inflammatory cytokines (IL-1β, IL-18), in which the order of cytotoxicity was dichlorvos > dipterex > dibrom > paraoxon > fenthion > dimethoate, based on the IC values of 274, 410, 551, 585, 2,158, and 1,527,566 μM, respectively. The findings suggest that targeting the NLRP3 inflammasome pathway could be a potential approach for developing broad-spectrum antitoxic drugs to combat multi-OPPs-induced toxicity. Moreover, inhibition of NLRP3 efficiently protected the cells against cytotoxicity induced by these six OPPs, and the expression of NLRP3, ASC, Caspase-1, IL-1β, and IL-18 decreased accordingly. The order of NLRP3 affinity for OPPs was dimethoate > paraoxon > dichlorvos > dibrom > (fenthion and dipterex) based on values of 89.8, 325, 1,460, and 2,690 μM, respectively. Furthermore, the common molecular mechanism of NLRP3-OPPs was clarified by the presence of toxicity effector groups (benzene ring, nitrogen/oxygen-containing functional group); =O, -O-, or =S (active) groups; and combination residues (Gly271, Asp272). This finding provided valuable insights into exploring the common mechanisms of multiple threats and developing effective therapeutic strategies to prevent OPPs poisoning.
PubMed: 38638206
DOI: 10.1029/2023GH000888 -
Environmental Analysis, Health and... Mar 2024For farmers around the world to protect crops from disturbing pests, it is common to use pesticides to ward off the growth of pests or even eliminate them. Even though...
For farmers around the world to protect crops from disturbing pests, it is common to use pesticides to ward off the growth of pests or even eliminate them. Even though pesticides are seen as a good thing for protecting crops, there is one thing that mustn't be forgotten the origin of the pesticide itself is a toxin compound that is dangerous if used irresponsibly. The main concern of this study is excessive use of pesticides may cause serious consequences to the ecosystem and environment through the accumulation of pesticide residue by irresponsible farmers. To minimize the effects of pesticide residues, the selection of the type of pesticide needs to be considered which type may not be harmful to the environment's health even though accumulation happens. Therefore, in this study, a fuzzy-based computational model assessor was built to measure the safety level of pesticides toward the environment. The fuzzy model was created with consideration of several parameters related to pesticide behaviors, its effects on beneficial organisms, and its persistence in the environment. The method used for this study includes literature reviewing, fuzzification, statistical approach, expert knowledge sharing, and quantitative analysis. The model created in this study can assist in a more accurate and realistic method of selecting better pesticide options that will be used by farmers. To ensure the validity of the model, verifying and validating the formula and pesticide result assessment were done with related literature articles. In this study, from 10 types of pesticides used as a sample, dodine, and iprodione pesticides are the best option for protecting crops with a safety level of 7.36, and abamectin, dimethoate, chorpyrifos, and methidathion are not safe options for farming use because of its potential of harming the environment.
PubMed: 38631395
DOI: 10.5620/eaht.2024003 -
Environmental Analysis, Health and... Mar 2024Developing countries like Nigeria are increasingly employing pesticides to boost the productivity of their agriculture and food supply, despite the fact that doing so...
Developing countries like Nigeria are increasingly employing pesticides to boost the productivity of their agriculture and food supply, despite the fact that doing so poses a health risk to the general populace. The purpose of this study was to assess pesticide residue levels in Lagos, Nigeria. A total of 18 samples from three neighbourhood markets were collected, and they were then examined for the presence of organochlorine (Endosulfan I, Pentachlorophenol, Heptachlor epoxide, and Endosulfan II) and organophosphate (Dichlorvos, Dimethoate, Phorate, and methyl parathion) residues. During the pre-treatment, the multi-residue Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS citrate) method with the addition of acetonitrile was used, and then samples were analysed using GC-MS. All of the samples contained dichlorvos, but the orange sample's concentration was below the limit of quantification, making quantification impossible. Dimethoate concentrations were below MRLs except waterleaf sample. With a concentration of 0.043 μg/mL, Waterleaf had the highest quantity of dimethoate in the sample. The findings of this study indicate that in order to safeguard the health of consumers, it is necessary to closely monitor organochlorine and organophosphate use nationwide, along with other related pesticides, and to test for pesticide residues in food products.
PubMed: 38631394
DOI: 10.5620/eaht.2024002 -
Foods (Basel, Switzerland) Mar 2024Dimethoate (DIM) as an organophosphorus pesticide is widely utilized especially in the cultivation of vegetables and fruits due to its killing effect on harmful insects....
A Novel Molecularly Imprinted Quartz Crystal Microbalance Sensor Based on Erbium Molybdate Incorporating Sulfur-Doped Graphitic Carbon Nitride for Dimethoate Determination in Apple Juice Samples.
Dimethoate (DIM) as an organophosphorus pesticide is widely utilized especially in the cultivation of vegetables and fruits due to its killing effect on harmful insects. However, unconscious use of DIM in large amounts can also cause serious health problems. For these reasons, rapid and reliable detection of DIM from food samples is significant. In this study, a novel quartz crystal microbalance (QCM) sensor based on erbium molybdate incorporating sulfur-doped graphitic carbon nitride (EM/S-g-CN) and a molecularly imprinting polymer (MIP) was designed for DIM detection in apple juice samples. Firstly, an EM/S-g-CN nanocomposite with high purity was prepared under hydrothermal conditions at high temperatures over a long period of time. After the modification of the EM/S-g-CN nanocomposite on a QCM chip, the polymerization solution including N,N'-azobisisobutyronitrile (AIBN) as an initiator, ethylene glycol dimethacrylate (EGDMA) as a cross-linker, methacryloylamidoglutamic acid (MAGA) as a monomer, and DIM as an analyte was prepared. Then, the polymerization solution was dropped on an EM/S-g-CN nanocomposite modified QCM chip and an ultraviolet polymerization process was applied for the formation of the DIM-imprinted polymers on the EM/S-g-CN nanocomposite modified QCM chip. After the polymerization treatment, some characterization studies, including electrochemical, microscopic, and spectroscopic methods, were performed to illuminate the surface properties of the nanocomposite and the prepared QCM sensor. The values of the limit of quantification (LOQ) and the detection limit (LOD) of the prepared QCM sensor were as 1.0 × 10 M and 3.3 × 10 M, respectively. In addition, high selectivity, stability, reproducibility, and repeatability of the developed sensor was observed, providing highly reliable analysis results. Finally, thanks to the prepared sensor, it may be possible to detect pesticides from different food and environmental samples in the future.
PubMed: 38472923
DOI: 10.3390/foods13050810 -
Biosensors Jan 2024Dimethoate contaminants in food pose a threat to human health. Rapid and sensitive trace detection methods are required to keep food safe. In this study, a novel...
Dimethoate contaminants in food pose a threat to human health. Rapid and sensitive trace detection methods are required to keep food safe. In this study, a novel fluorescent aptasensor was developed for the sensitive detection of dimethoate based on carbon quantum dots labeled with double-stranded DNA (CQDs-apt-cDNA) and TiCT flakes. Under optimal conditions, the aptasensor showed a good linear range of 1 × 10 to 5 × 10 M for dimethoate with a coefficient of determination (R) of 0.996. Besides, a low detection limit of 2.18 × 10 M was obtained. The aptasensor showed high selectivity in interference samples and good reproducibility with an RSD of 3.06% (<5%) for dimethoate detection. Furthermore, the proposed aptasensor was applied to the detection of dimethoate in apple juice and tap water with satisfactory recoveries from 96.2 to 104.4%. Because of these benefits, this aptasensor has the potential and promise for detecting food contaminants in the food industry.
Topics: Humans; Dimethoate; Pesticides; Reproducibility of Results; Titanium; Limit of Detection; Aptamers, Nucleotide; Biosensing Techniques; Nitrites; Transition Elements
PubMed: 38391988
DOI: 10.3390/bios14020069 -
Toxicology Reports Jun 2024Organophosphate insecticide spray poses potential threat of contamination of environmental components their accumulation in aquatic organisms. Although various...
Organophosphate insecticide spray poses potential threat of contamination of environmental components their accumulation in aquatic organisms. Although various physiological deficits associated with their exposure in fishes are documented, yet their retention in their edible muscle tissues has been poorly studied. In this context, the study was undertaken to ascertain the bioaccumulation of two organophosphate insecticide compounds (dimethoate and chlorpyrifos) in the muscles of juvenile . The study could provide insight into the risks to human health associated with consuming contaminated fish flesh. The fishes exposed to various concentrations of dimethoate and chlorpyrifos in-vivo for 96 to ascertain the uptake and retention of these insecticides in the muscle. Results indicated that fish muscles accumulated the residues at all the concentrations with the recovery of 2.99% (0.032 ppm) of dimethoate exposed to LC concentrations. In contrast, the chlorpyrifos residues were found Below the Detection Level (BDL) in the fishes exposed to LC concentrations. The percentage bioaccumulation of dimethoate in fish muscle was 88.10%, and that of chlorpyrifos was BDL. The bio-concentration factor was dose-dependent and increased with increasing doses of both insecticides. The study invites attention to human health risk assessment in the regions where contaminated fish are consumed without scientific supervision.
PubMed: 38379553
DOI: 10.1016/j.toxrep.2024.02.002 -
BMC Pharmacology & Toxicology Jan 2024Multiple pesticides are often used in combination for plant protection and public health. Therefore, it is important to analyze the physiological changes induced by...
BACKGROUND
Multiple pesticides are often used in combination for plant protection and public health. Therefore, it is important to analyze the physiological changes induced by multiple pesticides exposure. The objective of this study was to investigate the combined toxicity of the widely-used organophosphorus and pyrethroid pesticides diazinon, dimethoate, and cypermethrin.
METHODS
Male Wistar rats were administrated by gavage once daily with the three pesticides individual or in combination for consecutive 28 days. The metabolic components of serum and urine samples were detected by using H nuclear magnetic resonance (NMR)-based metabolomics method. Histopathological examination of liver and kidneys and serum biochemical determination were also carried out.
RESULTS
The results showed that after the 28-day subacute exposure, serum glutamic transaminase and albumin were significantly increased and blood urea nitrogen was significantly decreased in the rats exposed to the mixture of the pesticides compared with the control rats, suggesting that the co-exposure impaired liver and kidney function. Metabolomics analysis indicated that the indicators 14 metabolites were statistically significant altered in the rats after the exposure of the pesticides. The increase in 3-hydroxybutyric acid in urine or decrease of lactate and N-acetyl-L-cysteine in serum could be a potentially sensitive biomarker of the subchronic combined effects of the three insecticides. The reduction level of 2-oxoglutarate and creatinine in urine may be indicative of dysfunction of liver and kidneys.
CONCLUSION
In summary, the exposure of rats to pesticides diazinon, dimethoate, and cypermethrin could cause disorder of lipid and amino acid metabolism, induction of oxidative stress, and dysfunction of liver and kidneys, which contributes to the understanding of combined toxic effects of the pesticides revealed by using the metabolomics analysis of the urine and serum profiles.
Topics: Rats; Animals; Diazinon; Dimethoate; Rats, Wistar; Pyrethrins; Pesticides; Liver
PubMed: 38167230
DOI: 10.1186/s40360-023-00714-6 -
Chemosphere Jan 2024The worldwide and intensive use of phytosanitary compounds results in environmental and food contamination by chemical residues. Human exposure to multiple pesticide...
Low concentrations of ethylene bisdithiocarbamate pesticides maneb and mancozeb impair manganese and zinc homeostasis to induce oxidative stress and caspase-dependent apoptosis in human hepatocytes.
The worldwide and intensive use of phytosanitary compounds results in environmental and food contamination by chemical residues. Human exposure to multiple pesticide residues is a major health issue. Considering that the liver is not only the main organ for metabolizing pesticides but also a major target of toxicities induced by xenobiotics, we studied the effects of a mixture of 7 pesticides (chlorpyrifos-ethyl, dimethoate, diazinon, iprodione, imazalil, maneb, mancozeb) often detected in food samples. Effects of the mixture was investigated using metabolically competent HepaRG cells and human hepatocytes in primary culture. We report the strong cytotoxicity of the pesticide mixture towards hepatocytes-like HepaRG cells and human hepatocytes upon acute and chronic exposures at low concentrations extrapolated from the Acceptable Daily Intake (ADI) of each compound. Unexpectedly, we demonstrated that the manganese (Mn)-containing dithiocarbamates (DTCs) maneb and mancozeb were solely responsible for the cytotoxicity induced by the mixture. The mechanism of cell death involved the induction of oxidative stress, which led to cell death by intrinsic apoptosis involving caspases 3 and 9. Importantly, this cytotoxic effect was found only in cells metabolizing these pesticides. Herein, we unveil a novel mechanism of toxicity of the Mn-containing DTCs maneb and mancozeb through their metabolization in hepatocytes generating the main metabolite ethylene thiourea (ETU) and the release of Mn leading to intracellular Mn overload and depletion in zinc (Zn). Alteration of the Mn and Zn homeostasis provokes the oxidative stress and the induction of apoptosis, which can be prevented by Zn supplementation. Our data demonstrate the hepatotoxicity of Mn-containing fungicides at very low doses and unveil their adverse effect in disrupting Mn and Zn homeostasis and triggering oxidative stress in human hepatocytes.
Topics: Humans; Maneb; Manganese; Pesticides; Zineb; Fungicides, Industrial; Apoptosis; Oxidative Stress; Zinc; Hepatocytes; Ethylenes; Homeostasis
PubMed: 37923018
DOI: 10.1016/j.chemosphere.2023.140535 -
Anais Da Academia Brasileira de Ciencias 2023Although it is known that organophosphate insecticides are harmfull to aquatic ecosystems, oxidative damages caused by Dimethoate and Chlorpyrifos are not studied on...
Although it is known that organophosphate insecticides are harmfull to aquatic ecosystems, oxidative damages caused by Dimethoate and Chlorpyrifos are not studied on Arthrospira platensis Gomont. In this study, various Chlorpyrifos (0-150 µg mL-1) and Dimethoate (0-250 µg mL-1) concentrations were added to the culture medium in laboratory to evaulate growth rate, chlorophyll-a content and antioxidant parameters of A. platensis. Optical Density (OD560) and chlorophyll-a decreased compared to the control for seven days in both pesticide applications. Superoxide dismutase (SOD) activity increased at 50 µg mL-1 Chlorpyrifos concentration but it decreased at all concentrations. Although Ascorbate peroxidase (APX) and glutathione reductase (GR) activities increased with Chlorpyrifos application, they did not change with Dimethoate application. Malondialdehyde (MDA) amount decreased at 150 µg mL-1 Chlorpyrifos concentration but it increased in Dimethoate application. The H2O2 content were increased in both applications. Proline decreased in 50 and 75 µg mL-1 Chlorpyrifos concentrations and increased at 150 µg mL-1 concentration, while it increased at 25 µg mL-1 Dimethoate concentration. The results were tested at 0.05 significance level. These pesticides inhibit A. platensis growth and chlorophyll-a production and cause oxidative stress. The excessive use may affect the phytoplankton and have negative consequences in the aquatic ecosystem.
Topics: Insecticides; Chlorpyrifos; Dimethoate; Ecosystem; Hydrogen Peroxide; Oxidative Stress; Pesticides; Antioxidants; Chlorophyll; Chlorophyll A; Organophosphorus Compounds
PubMed: 37729300
DOI: 10.1590/0001-3765202320200463 -
Toxicology Reports Dec 2023This paper reviewed the published data on the levels of different pesticide residues in vegetables (tomato, eggplant, beans, gourds, cauliflower, cabbage, cucumber,... (Review)
Review
This paper reviewed the published data on the levels of different pesticide residues in vegetables (tomato, eggplant, beans, gourds, cauliflower, cabbage, cucumber, potato, carrot, onion, red chilli, red amaranth, lady's finger, spinach, coriander, and lettuce) from Bangladesh in the last decade. Vegetable production in Bangladesh has increased tremendously (37.63%) compared to the last decades, along with its pesticide use. The most observed pesticide groups used in vegetable production were organophosphorus, pyrethroids, carbamate, organochlorine, nereistoxin analogue group, and neonicotinoids. More specifically, chlorpyrifos, dimethoate, diazinon, and malathion were the most used pesticides. More than 29% of the vegetable samples (1577) were contaminated with pesticide residue; among the contaminated samples (458), most cases (73%) exceeded the maximum residue limits (MRLs). The pesticide-contaminated vegetables were cucumber (51%), tomato (41%), cauliflower (31%), miscellaneous vegetables (36%), eggplant (29%), beans (23%), cabbage (18%), and gourds (16%). Among the pesticide-contaminated samples, vegetables with above MRL were gourds (100%), beans (92), tomato (78%), eggplant (73%), miscellaneous vegetables (69%), cucumber (62%), cabbage (50%), cauliflower (50%) (p < 0.05). It was also observed that a single vegetable was often contaminated with multiple pesticides, and farmers did not follow a proper withdrawal period while using pesticides. Hazard quotation (HQ>1) was observed in adolescents and adults in tomato, eggplant, beans, cauliflower, cabbage, cucumber, lady's finger, lettuce, and coriander. There was no health risk observed (HQ<1) in gourds, potato, carrot, onion, red chilli, red amaranth, spinach, and okra. The highest acute and chronic HQ (aHQ, cHQ) was observed for cypermethrin (bean) in adolescents (aHQ=255, cHQ= 510) and adults (aHQ=131, cHQ=263). It was also observed that these pesticides harmed air, soil, water, and non-target organisms. Nevertheless, the review will help the government develop policies that reduce pesticide use and raise people's awareness of its harmful effects.
PubMed: 37711360
DOI: 10.1016/j.toxrep.2023.09.003