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Journal of Environmental Health Science... Dec 2020In this study, the culturable halophilic and halotolerant bacterial diversity was determined in Aran-Bidgol as a thalassohaline seasonal hypersaline lake in Iran.
PURPOSE
In this study, the culturable halophilic and halotolerant bacterial diversity was determined in Aran-Bidgol as a thalassohaline seasonal hypersaline lake in Iran.
METHODS
Thirty water, soil, sediments, coastal mud, multi-color brines and salt crystals samples were extracted and cultured using different media and incubation conditions. Totally 958 isolates were obtained and 87 isolates were selected for further studies, based on morphological, physiological and biochemical tests, representing different morphotypes.
RESULTS
Based on 16S rRNA gene sequence analyses, the isolates exhibited 94.6-100% sequence similarity to the closest known species of the genera , , , , , , , , , , , , , , , , , , , , , , , , , , and and also, comparison of ARDRA patterns among the sequenced strains, using I, UI and II enzymes showed that these patterns are in accordance with the phylogenetic position of these strains.
CONCLUSION
The PCR-RFLP analyses suggested that ARDRA possess a functional potential for distinguishing halophilic bacteria to be used for further studies in elementary steps of isolation to reduce the tedious duplication of isolates.
PubMed: 33312616
DOI: 10.1007/s40201-020-00519-3 -
Journal, Genetic Engineering &... Oct 2020Halophiles offer an attractive source of genes conferring salt tolerance. Halobacillus trueperi SS1 strain of Lunsu, Himachal Pradesh, India, a strict halophile, was...
BACKGROUND
Halophiles offer an attractive source of genes conferring salt tolerance. Halobacillus trueperi SS1 strain of Lunsu, Himachal Pradesh, India, a strict halophile, was exploited to isolate and clone the genes for salt tolerance. The genomic library of BamH1 digest of H. trueperi SS1 was constructed in pUC19, and recombinants were screened for salt tolerance on an LB medium containing ampicillin (100 μg/ml) and NaCl (0 to 1.5 M).
RESULTS
One recombinant clone named as salt-tolerant clone (STC) conferred salt tolerance to host Escherichia coli/DH5α, which showed growth in the LB medium supplemented with ampicillin and 1.2 M NaCl. Restriction digestion and PCR analysis revealed the presence of an insert of approximately 2000 bp in the STC. DNA sequencing of the 2-kb insert on both strands yielded a sequence of 2301 nucleotides. Protein BLAST analysis of 2301-bp sequence of H. trueperi SS1 present in STC showed 97% identity to multidrug transport ATP binding/permease protein of Halobacillus karajensis. The insert contained in STC was subcloned into pGEX4T2 vector, and the recombinant clone STC/pGEX4T2 conferred salt tolerance to the bacterial host E. coli.
CONCLUSIONS
The present study led to the isolation of salt tolerance gene encoding a putative multidrug transport ATP binding/permease protein from H. trueperi SS1. The salt tolerance gene can be subcloned for transferring salt tolerance traits into agricultural crop plants for cultivation in saline and coastal lands.
PubMed: 33025336
DOI: 10.1186/s43141-020-00070-6 -
New Microbes and New Infections Sep 2020Strain Marseille-Q1234 is a new species from the genus that was isolated in 2019 from a stool sample in a healthy Malian child <5 years old. Cells are Gram-positive and...
Strain Marseille-Q1234 is a new species from the genus that was isolated in 2019 from a stool sample in a healthy Malian child <5 years old. Cells are Gram-positive and strictly halophilic bacilli. Strain Marseille-Q1234 exhibits 98.46% 16S rRNA gene sequence similarity to strain JSM 071068 (NR_116505.1), the phylogenetically closely related species with standing in nomenclature. Based on the phenotypic and phylogenetic evidence, OrthoANI values and results of the biochemical tests, the new species is named sp. nov., for which strain Marseille-Q1234 (= CSURQ1234) is proposed as the type strain.
PubMed: 32793353
DOI: 10.1016/j.nmni.2020.100708 -
Marine Drugs Jul 2019Quorum sensing (QS) antagonists have been proposed as novel therapeutic agents to combat bacterial infections. We previously reported that the secondary metabolite...
Quorum sensing (QS) antagonists have been proposed as novel therapeutic agents to combat bacterial infections. We previously reported that the secondary metabolite 3-methyl--(2'-phenylethyl)-butyramide, produced by a marine bacterium identified as , inhibits QS controlled phenotypes in multiple Gram-negative reporter strains. Here we report that -phenethyl hexanamide, a structurally-related compound produced by the marine bacterium , similarly demonstrates QS inhibitory properties. To more fully explore structure-activity relationships within this new class of QS inhibitors, a panel of twenty analogs was synthesized and biologically evaluated. Several compounds were identified with increased attenuation of QS-regulated phenotypes, most notably -(4-fluorophenyl)-3-phenylpropanamide against the marine pathogen (IC = 1.1 µM). These findings support the opportunity to further develop substituted phenethylamides as QS inhibitors.
Topics: Amides; Anti-Bacterial Agents; Halobacillus; Inhibitory Concentration 50; Quorum Sensing; Secondary Metabolism; Structure-Activity Relationship; Vibrio
PubMed: 31266202
DOI: 10.3390/md17070389 -
Marine Drugs May 2019The secondary metabolite Tyrian purple, also known as shellfish purple and royal purple, is a dye with historical importance for humans. The biosynthetic origin of...
The secondary metabolite Tyrian purple, also known as shellfish purple and royal purple, is a dye with historical importance for humans. The biosynthetic origin of Tyrian purple in Muricidae molluscs is not currently known. A possible role for symbiotic bacteria in the production of tyrindoxyl sulphate, the precursor to Tyrian purple stored in the Australian species, , has been proposed. This study aimed to culture bacterial symbionts from the purple producing hypobranchial gland, and screen the isolates for bromoperoxidase genes using molecular methods. The ability of bromoperoxidase positive isolates to produce the brominated indole precursor to Tyrian purple was then established by extraction of the culture, and analysis by liquid chromatography-mass spectrometry (LC-MS). In total, 32 bacterial isolates were cultured from hypobranchial glands, using marine agar, marine agar with hypobranchial gland aqueous extracts, blood agar, thiosulphate citrate bile salts sucrose agar, and cetrimide agar at pH 7.2. These included 26 spp., two spp., one sp., one sp., one sp. and one sp. The two species were the only isolates found to have coding sequences for bromoperoxidase enzymes. LC-MS analysis of the supernatant and cell pellets from the bromoperoxidase producing spp. cultured in tryptone broth, supplemented with KBr, confirmed their ability to produce the brominated precursor to Tyrian purple, tyrindoxyl sulphate. This study supports a potential role for symbiotic spp. in the biosynthesis of Tyrian purple.
Topics: Animals; Bacillus; Bacteria; Branchial Region; Gastropoda; Indoles; Mollusca; Peroxidases; Sequence Analysis, RNA; Symbiosis
PubMed: 31058830
DOI: 10.3390/md17050264 -
Microbiology Resource Announcements Mar 2019We report here the genome sequence of halophilic Halobacillus trueperi SS1, isolated from the Lunsu saltwater body in India. The bacteria are Gram positive and rod...
We report here the genome sequence of halophilic Halobacillus trueperi SS1, isolated from the Lunsu saltwater body in India. The bacteria are Gram positive and rod shaped. The genome of H. trueperi SS1 has 4.14 Mbp, with 4,329 coding sequences, 35 RNA genes (29 tRNAs, 2 rRNAs, and 4 noncoding RNAs), and 42.15% G+C content.
PubMed: 30863831
DOI: 10.1128/MRA.01710-18 -
Scientific Reports Feb 2019Here we report the biological synthesis of CdS fluorescent nanoparticles (Quantum Dots, QDs) by polyextremophile halophilic bacteria isolated from Atacama Salt Flat...
Here we report the biological synthesis of CdS fluorescent nanoparticles (Quantum Dots, QDs) by polyextremophile halophilic bacteria isolated from Atacama Salt Flat (Chile), Uyuni Salt Flat (Bolivia) and the Dead Sea (Israel). In particular, a Halobacillus sp. DS2, a strain presenting high resistance to NaCl (3-22%), acidic pH (1-4) and cadmium (CdCl MIC: 1,375 mM) was used for QDs biosynthesis studies. Halobacillus sp. synthesize CdS QDs in presence of high NaCl concentrations in a process related with their capacity to generate S in these conditions. Biosynthesized QDs were purified, characterized and their stability at different NaCl concentrations determined. Hexagonal nanoparticles with highly defined structures (hexagonal phase), monodisperse size distribution (2-5 nm) and composed by CdS, NaCl and cysteine were determined by TEM, EDX, HRXPS and FTIR. In addition, QDs biosynthesized by Halobacillus sp. DS2 displayed increased tolerance to NaCl when compared to QDs produced chemically or biosynthesized by non-halophilic bacteria. This is the first report of biological synthesis of salt-stable QDs and confirms the potential of using extremophile microorganisms to produce novel nanoparticles. Obtained results constitute a new alternative to improve QDs properties, and as consequence, to increase their industrial and biomedical applications.
Topics: Cadmium Compounds; Extremophiles; Halobacillus; Nanoparticles; Quantum Dots; Sodium Chloride; Spectrometry, Fluorescence; Sulfides
PubMed: 30760793
DOI: 10.1038/s41598-018-38330-8 -
Brazilian Journal of Microbiology :... Jan 2019Microbes from hypersaline environments are useful in biotechnology as sources of novel enzymes and proteins. The current study aimed to characterize halophilic bacteria...
Microbes from hypersaline environments are useful in biotechnology as sources of novel enzymes and proteins. The current study aimed to characterize halophilic bacteria from the rhizosphere of halophytes (Salsola stocksii and Atriplex amnicola), non-rhizospheric, and brine lake-bank soils collected from Khewra Salt Mine and screening of these bacterial strains for industrially important enzymes. A total of 45 bacterial isolates from the rhizosphere of Salsola, 38 isolates from Atriplex, 24 isolates from non-rhizospheric, and 25 isolates from lake-bank soils were identified by using 16S rRNA gene analysis. Phylogenetic analysis showed that bacterial strains belonging to Bacillus, Halobacillus, and Kocuria were dominant in the rhizosphere of halophytes (Salsola and Atriplex), and Halobacillus and Halomonas were dominating genera from non-rhizospheric and lake-bank soils. Mostly identified strains were moderately halophilic bacteria with optimum growth at 1.5-3.0 M salt concentrations. Most of the bacterial exhibited lipase, protease, cellulase, amylase, gelatinase, and catalase activities. Halophilic and halotolerant Bacilli (AT2RP4, HL1RS13, NRS4HaP9, and LK3HaP7) identified in this study showed optimum lipase, protease, cellulase, and amylase activities at 1.0-1.5 M NaCl concentration, pH 7-8, and temperature 37 °C. These results indicated that halophilic and halotolerant bacteria can be used for bioconversion of organic compounds to useful products under extreme conditions.
Topics: Atriplex; Bacteria; Bacterial Proteins; Biodiversity; Cellulases; Hydrolases; Lakes; Lipase; Peptide Hydrolases; Phylogeny; Rhizosphere; Sodium Chloride; Soil Microbiology
PubMed: 30623303
DOI: 10.1007/s42770-019-00044-y -
Journal of Microbiology and... Dec 2018A total of 108 marine bacteria were isolated from the deep sea sediment of Bay of Bengal, India. Of which, 15 bacteria showed antimicrobial activity against human...
A total of 108 marine bacteria were isolated from the deep sea sediment of Bay of Bengal, India. Of which, 15 bacteria showed antimicrobial activity against human pathogenic bacteria. These antagonistic marine bacteria were characterized phenotypically and their taxonomic affiliations were made on the basis of 16S rRNA gene homology and molecular phylogeny tree analysis, the antagonistic marine bacteria were identified as the species of , , , . The extracts of potent marine bacteria exhibited differential cytotoxicity towards lung (A549), cervical (HeLa), breast (MCF-7) and colon (HT-29) cancer cells. The acridine-orange and ethidium bromide (AO/EB) staining of the extract of strain MB30-treated cancer cells showed typical characteristics of apoptosis such as nuclear condensation, cell shrinkage and formation of apoptotic bodies. Present investigation, reports potent marine bacteria from the deep sea sediment of Bay of Bengal that exhibit broad-spectrum antimicrobial and cytotoxicity potential. Due to their innate bioactive potential, these bacteria can be used as the source of potent molecules.
PubMed: 30602267
DOI: 10.4014/jmb.1811.11010 -
Data in Brief Dec 2018We present the draft genome sequence of sp. BBL2006, a moderately halophilic, gram positive bacterium isolated from a sulfidic salt spring in Big Bone Lick State Park,...
We present the draft genome sequence of sp. BBL2006, a moderately halophilic, gram positive bacterium isolated from a sulfidic salt spring in Big Bone Lick State Park, Boone County, Kentucky. The genome of sp. BBL2006 was 3,988,138 bp in length with a GC content of 41.6%. Genome analysis identified 4331 open reading frames including genes for antibiotic resistance and tolerance to heavy metals. The draft genome was deposited at DDBJ/EMBL/GenBank (DNA Databank of Japan/European Molecular Biology Laboratory/Genbank) (JRNX00000000).
PubMed: 30547067
DOI: 10.1016/j.dib.2018.11.076