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The Pan African Medical Journal 2018We here report the case of a 58-year old patient admitted with respiratory distress, oxygen desaturation 65% while breathing ambient air and fever 38.5°C. Blood count...
We here report the case of a 58-year old patient admitted with respiratory distress, oxygen desaturation 65% while breathing ambient air and fever 38.5°C. Blood count showed pancytopenia. Ionogram objectified slight hepatic cytolysis and chest X-ray showed bilateral miliaria. BK research using sputum was negative as well as Genexpert TB test. Anatomopathological examination of the osteomedullar biopsy revealed epitheliogigantocellular granuloma without caseous necrosis and polymerase chain reaction (PCR) showed mycobacterium tuberculosis in osteomedullary biopsy. Liver biopsy showed epithelioid granuloma without caseous necrosis. The diagnosis of multifocal tuberculosis with pulmonary, hematopoietic and hepatic impairment was retained. Miliary Tuberculosis is a severe form of tuberculosis. Bacilloscopies are often negative and the diagnosis can be retained based on other sampling, including cerebrospinal fluid sampling and osteomedullary biopsy. Different kinds of biopsies enable diagnosis in rare cases. PCR, a technique used in molecular biology, has enabled to shorten the window period from disease onset to diagnosis as well as treatment implementation. Miliary tuberculosis can be life-threatening; therefore, sometimes, treatment must be administered before certain diagnosis.
Topics: Biopsy; Female; Granuloma; Humans; Middle Aged; Mycobacterium tuberculosis; Pancytopenia; Polymerase Chain Reaction; Tuberculosis, Hepatic; Tuberculosis, Miliary; Tuberculosis, Pulmonary
PubMed: 31011393
DOI: 10.11604/pamj.2018.31.92.17046 -
Molecular & Cellular Proteomics : MCP Jul 2019The eccrine sweat gland is an exocrine gland that is involved in the secretion of sweat for control of temperature. Malfunction of the sweat glands can result in...
The eccrine sweat gland is an exocrine gland that is involved in the secretion of sweat for control of temperature. Malfunction of the sweat glands can result in disorders such as miliaria, hyperhidrosis and bromhidrosis. Understanding the transcriptome and proteome of sweat glands is important for understanding their physiology and role in diseases. However, no systematic transcriptome or proteome analysis of sweat glands has yet been reported. Here, we isolated eccrine sweat glands from human skin by microdissection and performed RNA-seq and proteome analysis. In total, ∼138,000 transcripts and ∼6,100 proteins were identified. Comparison of the RNA-seq data of eccrine sweat glands to other human tissues revealed the closest resemblance to the cortex region of kidneys. The proteome data showed enrichment of proteins involved in secretion, reabsorption, and wound healing. Importantly, protein level identification of the calcium ion channel TRPV4 suggests the importance of eccrine sweat glands in re-epithelialization of wounds and prevention of dehydration. We also identified 2 previously missing proteins from our analysis. Using a proteogenomic approach, we identified 7 peptides from 5 novel genes, which we validated using synthetic peptides. Most of the novel proteins were from short open reading frames (sORFs) suggesting that many sORFs still remain to be annotated in the human genome. This study presents the first integrated analysis of the transcriptome and proteome of the human eccrine sweat gland and would become a valuable resource for studying sweat glands in physiology and disease.
Topics: Amino Acid Sequence; Eccrine Glands; Exons; Humans; Open Reading Frames; Proteome; Proteomics; Pseudogenes; RNA; Transcriptome
PubMed: 30979791
DOI: 10.1074/mcp.RA118.001101 -
Biologia 2019The expansion of energy crops such as miscanthus has changed the habitat of European farmland birds. However, most studies on the subject are based in Western Europe....
The expansion of energy crops such as miscanthus has changed the habitat of European farmland birds. However, most studies on the subject are based in Western Europe. We surveyed the avian community in a sample bioenergy landscape in Poland to investigate the pattern of use of the crop by birds in Central Europe. During a year-long survey, 80 species were noted, with 32 sighted in miscanthus. The number of bird sightings was negatively correlated with the area covered by miscanthus in study plots. In contrast to results from Western Europe, most woodland-generalist bird species tended to avoid miscanthus. Farmland species (e.g. the corn bunting ; the tree sparrow ; the starling ; the yellowhammer ) were less frequently sighted in the crop than in other habitats. Only a few species were sighted more often in miscanthus than in other habitats, e.g. the marsh warbler ; the reed bunting ; the whinchat . The potential impact of expanded miscanthus cropping on bird communities in Central and Eastern Europe may diverge from predictions derived from studies based in Western Europe due to differing habitat preferences and bird densities between the regions. For a majority of farmland species, the crop may constitute only a suboptimal, supplementary habitat, with only a few birds potentially adapting to the exploitation of bioenergy plantations.
PubMed: 30636777
DOI: 10.2478/s11756-018-0143-1 -
The Pan African Medical Journal 2018Prickly heat is a benign cutaneous manifestation due to the obstruction of the sweat ducts following excessive exposure to heat, humidity or hyperthermia. We report the...
Prickly heat is a benign cutaneous manifestation due to the obstruction of the sweat ducts following excessive exposure to heat, humidity or hyperthermia. We report the case of a 70-year old diabetic female patient treated with insulin, hospitalized in the Intensive Care Unit due to acidocetosic coma secondary to pyelonephritis. The patient had diffuse clear vesicular lesions spread all over the body,(a) firm to palpation, on a healthy skin, suggesting miliaria crystallina. Miliaria crystallina is a benign cutaneous manifestation due to sweat retention secondary to obstruction of the sweat ducts. It is possible to distinguish between miliaria crystallina due to the obstruction in the stratum corneum, miliaria rubra due to the obstruction within the malpighian stratum and miliaria profunda due to an obstrusction in or below the dermoepidermal junction, depending on the level of obstruction. Miliaria crystallina heals spontaneously in a few hours giving rise to a desquamation, as occurred in our patient after regression of febrile syndrome.
Topics: Aged; Diabetes Mellitus; Diagnosis, Differential; Female; Hospitalization; Humans; Hypoglycemic Agents; Insulin; Miliaria
PubMed: 30344853
DOI: 10.11604/pamj.2018.30.69.15383 -
Anais Brasileiros de Dermatologia 2018Fox-Fordyce disease is a relatively infrequent pathology of the apocrine glands that affects almost exclusively young women. The disease is characterized by the presence...
Fox-Fordyce disease is a relatively infrequent pathology of the apocrine glands that affects almost exclusively young women. The disease is characterized by the presence of pruritic follicular papules mainly in the armpits that respond poorly to treatment and severely affect the patient's quality of life. We report two cases with clinical diagnosis and histopathological confirmation, presenting perifollicular xanthomatosis on histological examination, recently described as a distinctive, consistent, and specific feature of this disease.
Topics: Adult; Female; Fox-Fordyce Disease; Humans; Immunohistochemistry; Xanthomatosis; Young Adult
PubMed: 30066765
DOI: 10.1590/abd1806-4841.20187475 -
Anais Brasileiros de Dermatologia 2018
Topics: Adult; Axilla; Biopsy; Dermis; Female; Fox-Fordyce Disease; Hair Follicle; Humans; Vulva
PubMed: 29641729
DOI: 10.1590/abd1806-4841.20187348 -
Indian Journal of Dermatology,... 2018
Topics: Antibiotics, Antineoplastic; Doxorubicin; Female; Humans; Lichenoid Eruptions; Liposomes; Miliaria; Polyethylene Glycols; Young Adult
PubMed: 28513486
DOI: 10.4103/0378-6323.206233 -
The Pan African Medical Journal 2016
PubMed: 28292125
DOI: 10.11604/pamj.2016.25.163.9745 -
Polish Journal of Pathology : Official... 2017President of prewar Lvov and Polish Republic on Exile, associate professor Stanisław Ostrowski was a dermatologist with a keen interest in dermatopathology. This study...
President of prewar Lvov and Polish Republic on Exile, associate professor Stanisław Ostrowski was a dermatologist with a keen interest in dermatopathology. This study was based on original resources, which - mainly reports of his own authorship - were focused on dermatopathology. Stanisław Ostrowski provided excellent description of naevus epitheliomatosus sebaceus Wolters-Friboes both in Polish and German to be cited after decades in renowned handbooks of dermatopahtology published by Springer Verlag. His scientific output also includes meticulous presentation of Fox-Fordyce disease (apocrine miliaria) as well as gold-induced skin changes to Polish readership. Thus, this study documents dermatopahtological achievements of Stanisław Ostrowski - the unifying statesman of society of Lvov and Polish emigration in London.
Topics: Biopsy; Dermatology; Emigrants and Immigrants; Emigration and Immigration; History, 20th Century; Humans; Military Personnel; Nevus; Pathology; Poland; Sebaceous Gland Neoplasms
PubMed: 29517196
DOI: 10.5114/pjp.2017.73919 -
The Journal of Investigative Dermatology Jan 2017Sweat glands are critical for thermoregulation. The single tubular structure of sweat glands has a lower secretory portion and an upper reabsorptive duct leading to the...
Sweat glands are critical for thermoregulation. The single tubular structure of sweat glands has a lower secretory portion and an upper reabsorptive duct leading to the secretory pore in the skin. Genes that determine sweat gland structure and function are largely unidentified. Here we report that a Fox family transcription factor, Foxc1, is obligate for appreciable sweat duct activity in mice. When Foxc1 was specifically ablated in skin, sweat glands appeared mature, but the mice were severely hypohidrotic. Morphologic analysis revealed that sweat ducts were blocked by hyperkeratotic or parakeratotic plugs. Consequently, lumens in ducts and secretory portions were dilated, and blisters and papules formed on the skin surface in the knockout mice. The phenotype was strikingly similar to the human sweat retention disorder miliaria. We further show that Foxc1 deficiency ectopically induces the expression of keratinocyte terminal differentiation markers in the duct luminal cells, which most likely contribute to keratotic plug formation. Among those differentiation markers, we show that Sprr2a transcription is directly repressed by overexpressed Foxc1 in keratinocytes. In summary, Foxc1 regulates sweat duct luminal cell differentiation, and mutant mice mimic miliaria and provide a possible animal model for its study.
Topics: Animals; Biopsy, Needle; Cell Differentiation; Cells, Cultured; Female; Fluorescent Antibody Technique; Forkhead Transcription Factors; Fox-Fordyce Disease; Gene Expression Regulation, Developmental; Hepatocyte Nuclear Factor 3-alpha; Humans; Hypohidrosis; Immunohistochemistry; Keratinocytes; Mice; Mice, Inbred C57BL; Mice, Knockout; Models, Animal; RNA, Messenger; Real-Time Polymerase Chain Reaction; Reference Values; Sweat Glands
PubMed: 27592801
DOI: 10.1016/j.jid.2016.08.012