-
Ecotoxicology and Environmental Safety Jul 2023As an environmental pollutant, profenofos (PFF) can seriously endanger human health through the food chain. Albicanol is a sesquiterpene compound with antioxidant,...
As an environmental pollutant, profenofos (PFF) can seriously endanger human health through the food chain. Albicanol is a sesquiterpene compound with antioxidant, anti-inflammatory, and anti-aging properties. Previous studies have shown that Albicanol can antagonize apoptosis and genotoxicity caused by PFF exposure. However, the toxicity mechanism of PFF regulating hepatocyte immune function, apoptosis, and programmed necrosis and the role of Albicanol in this process have not been reported yet. In this study, grass carp hepatocytes (L8824) were treated with PFF (200 μM) or combined with Albicanol (5 ×10 μg mL) for 24 h to establish an experimental model. The results of JC-1 probe staining and Fluo-3 AM probe staining showed increased free calcium ions and decreased mitochondrial membrane potential in L8824 cells after PFF exposure, suggesting that PFF exposure may lead to mitochondrial damage. Real-time quantitative PCR and Western blot results showed that PFF exposure could increase the transcription of innate immunity-related factors (C3, Pardaxin 1, Hepcidin, INF-γ, IL-8, and IL-1β) in L8824 cells. PFF up-regulated the TNF/NF-κB signaling pathway and the expression of caspase-3, caspase-9, Bax, MLKL, RIPK1, and RIPK3 and down-regulated the expression of Caspase-8 and Bcl-2. Albicanol can antagonize the above-mentioned effects caused by PFF exposure. In conclusion, Albicanol antagonized the mitochondrial damage, apoptosis, and necroptosis of grass carp hepatocytes caused by PFF exposure by inhibiting the TNF/NF-κB pathway in innate immunity.
Topics: Humans; Animals; NF-kappa B; Immunity, Innate; Apoptosis; Sesquiterpenes; Carps
PubMed: 37196524
DOI: 10.1016/j.ecoenv.2023.115014 -
Biosensors Feb 2023Chlorpyrifos (CHL), profenofos (PRO) and cypermethrin (CYP) are widely used in combination to increase crop yields. However, these three pesticides can cause serious...
Chlorpyrifos (CHL), profenofos (PRO) and cypermethrin (CYP) are widely used in combination to increase crop yields. However, these three pesticides can cause serious harm to human health and do not easily degrade. In this study, a novel visible paper sensor has been prepared successfully and different colorimetric reactions were utilized to detect the three pesticides simultaneously. The sensor was constructed by grafting a zwitterionic polymer onto a cellulose filter (CF) and placing it on a glass surface modified with PDMS. The branch shape was designed to form multiple detection areas, which were modified with specific pesticides and corresponding chromogenic reagents. The as-prepared colorimetric platform exhibited high sensitivity, a short detection time, a good linear response and a low detection limit (LOD) for the three pesticides (chlorpyrifos: y = 46.801 - 1.939x, R = 0.983, LOD = 0.235 mg/L; profenofos: y = 40.068 + 42.5x, R = 0.988, LOD = 4.891 mg/L; cypermethrin: y = 51.993 + 1.474x, R = 0.993, LOD = 4.053 mg/L). The comparison of the results obtained by the proposed paper sensor and those obtained by spectrophotometry further revealed the stability and reliability of the paper sensor. In particular, the color intensity of the interaction between the pesticides and coloring agents could be directly observed by the human eye. The consistency of the colorimetric/optical assay was proven in real target pesticide samples. Thus, this sensing strategy provides a portable, cost-effective, accurate and visualized paper platform, which could be suitable for application in the fruit and vegetable industry for monitoring CHL, PRO and CYP in parallel.
Topics: Humans; Pesticides; Chlorpyrifos; Colorimetry; Reproducibility of Results
PubMed: 36979521
DOI: 10.3390/bios13030309 -
Frontiers in Nutrition 2023Carbendazim (CBZ) is a broad-spectrum fungicide, which is toxic to mammals. Therefore, it is very necessary to establish a sensitive detection for food safety. An...
Carbendazim (CBZ) is a broad-spectrum fungicide, which is toxic to mammals. Therefore, it is very necessary to establish a sensitive detection for food safety. An experiment found that CD exhibited excellent catalysis for the nano-indicator reaction of HAuCl-glyoxal to produce gold nanoparticles (AuNPs) and that the generated AuNPs have a very strong surface-enhanced Raman scattering (SERS) effect at 1613 cm in the presence of Victoria blue B molecular probes, and resonance Rayleigh scattering (RRS) signals at 370 nm. The aptamer (Apt) suppressed the catalysis of CD to cause the SERS and RRS signals decreasing. With the addition of CBZ, the specific Apt reaction occurred to restore the catalysis of CD, and resulting in a linear increase in the signals of RRS and SERS. As a result, this new nanocatalytic amplification indicator reaction was coupled with a specific Apt reaction of carbendazim (CBZ), to construct a new CD catalytic amplification-aptamer SERS/RRS discattering assay for ultratrace CBZ, which was used to analyze CBZ in tea samples with satisfactory results. In addition, this biosensoring platform can be also used to assay profenofos.
PubMed: 36950331
DOI: 10.3389/fnut.2023.1122876 -
Heliyon Mar 2023In this study, analysis of some selected organophosphorus and organochlorine pesticides was carried out in tomato samples collected from the markets of Mettu town, Ilu...
In this study, analysis of some selected organophosphorus and organochlorine pesticides was carried out in tomato samples collected from the markets of Mettu town, Ilu Aba Bora Zone, Oromia, Ethiopia. The quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) method was used for the extraction of collected samples using acetonitrile with 1% acetic acid, anhydrous magnesium sulfate, and anhydrous sodium acetate, followed by dispersive solid-phase extraction (d-SPE). Chromatographic separation and quantitative determination of the pesticides were performed by gas chromatography equipped with a mass spectrometer. The calibration curves for each analyte constructed in tomato samples were linear in the concentration range of 2-200 μg/kg with R > 0.99 and the LODs and LOQs of the method were in the range of 0.01-0.04 μg/kg and 0.04-0.13 μg/kg, respectively. The recovery and precision study results were within acceptable standard guidelines. Of the fourteen pesticides tested, chlordane, hexachlorobenzene, 4, 4'-DDE, 4, 4'-DDD, 4, 4'- DDT, and profenofos were detected at 0.15, 0.09, 0.17, 0.032, 1.24 and 0.062 μg/kg, respectively. Despite the concentrations of the detected pesticides being lower than the maximum residue limit recommended by FAO/WHO, the presence of these toxic chemicals in tomatoes indicates product contamination and requires extensive further investigation with the inclusion of other pesticides.
PubMed: 36923857
DOI: 10.1016/j.heliyon.2023.e14121 -
Environmental Analysis, Health and... Dec 2022The aim of the study was to assess the occurrence and distribution of organophosphate compounds residue in soil, surface water, sediment, and banana crops in Araromi...
The aim of the study was to assess the occurrence and distribution of organophosphate compounds residue in soil, surface water, sediment, and banana crops in Araromi farm settlement, Osun State, Nigeria. Organophosphate pesticide residues were determined using a gas chromatography equipped with Flame-Ionization Detection (GC-FID) in 16 soil samples from cocoa and banana farms, 6 water and sediment samples each, and 8 banana samples from 4 farms in the study site. Fourteen organophosphate compounds were detected (acephate, omethoate, dementon-s-methyl, dimethoate, tolcofos-methyl, pirimiphos-methyl, malathion, chlorpyrifos, methidathion, prothiofos, profenofos, ethion, azinphos-methyl and pyrazophos). Tolclofos-methyl, pirimiphos-methyl and prothiofos were detected in all the soil and sediment samples with concentration ranges of 1.9-12.9, 2.25-6.98 and 3.38-9.89 mg/kg respectively in soil and 8.13-9.83, 2.82-25.1 and 3.70-19.5 mg/kg respectively in sediment. Dimethoate, pirimiphos-methyl and prothiofos with concentration ranges, 0.06-0.28, 0.09-0.18 and 0.16-6.11 mg/L respectively were mostly detected in water samples while dimethoate, tolcofos-methyl, malathion, methidathion, prothiofos, ethion and azinphos-methyl compounds were detected in all the banana samples with concentration ranges, 3.40-12.0, 1.82-6.26, 5.73-9.48, 29.7-145, 8.24-20.1, 3.87-9.35 and 3.66-12.2 mg/kg respectively. The organophosphate mean residue concentrations were mostly significantly higher than the Maximum Residue Limits (MRL) at p<0.05. Across the three samples, only pirimiphos-methyl was significantly higher in water samples, omethoate in sediment; acephate, dementon-s-methyl and chlorpyrifos in banana were also not significantly higher at p<0.05. A strong positive significant correlation was observed between the organophosphate compounds in the banana and water samples (R=0.77, p=0.002) at p<0.05. The occurrence of organophosphate compounds in concentrations above MRLs may pose serious environmental and health risks.
PubMed: 36916048
DOI: 10.5620/eaht.2022035 -
Frontiers in Physiology 2023Synthetic insecticides have been an inevitable part of plant protection throughout the world. Sublethal effects of these chemicals on beneficial insect species are one...
Sublethal and transgenerational effects of synthetic insecticides on the biological parameters and functional response of (Coleoptera: Coccinellidae) under laboratory conditions.
Synthetic insecticides have been an inevitable part of plant protection throughout the world. Sublethal effects of these chemicals on beneficial insect species are one of the contemporary issues these days. Using the age-stage, two-sex life table model, this study evaluated the sublethal and transgenerational effects of six synthetic insecticides (imidacloprid, thiamethoxam, lambda-cyhalothrin, cypermethrin, chlorpyrifos and profenofos) commonly applied to winter vegetables, on the fitness and predation of the seven-spotted ladybeetle, , which is an efficient predator of aphids worldwide. According to results, all insecticides at their sublethal doses (LC) significantly suppressed the emergence of adults, adult weight, fertility and fecundity of the parental generation compared to control treatment. The larval stage was prolonged and oviposition, fecundity and total longevity of the adult beetles were decreased in unexposed progeny whose parents were exposed to sublethal doses of all insecticides. Moreover, the biological parameters of adults, including the intrinsic rate of increase (), finite rate of increase () and net reproductive rate ( ) were significantly reduced when exposed to sublethal doses of insecticides. The predation rate of the F generation adults was also decreased after exposure to the sublethal doses of insecticides. However, chlorpyrifos, profenofos, lambda-cyhalothrin and cypermethrin exhibited more deleterious effects on the fitness and population parameters of beetles than imidacloprid and thiamethoxam.
PubMed: 36726846
DOI: 10.3389/fphys.2023.1088712 -
Ecotoxicology (London, England) Mar 2023Profenofos (organophosphate) is among the major toxicant polluting freshwater bodies, exerting a significant effect on fish health. The LC value of Profenofos (PRO) was...
Toxicity bioassay and sub-lethal effects of profenofos-based insecticide on behavior, biochemical, hematological, and histopathological responses in Grass carp (Ctenopharyngodon idella).
Profenofos (organophosphate) is among the major toxicant polluting freshwater bodies, exerting a significant effect on fish health. The LC value of Profenofos (PRO) was resolved in Grass carp (Ctenopharyngodon idella) with average body weight (55.82 ± 5.42 g) and determined the 96 h LC value as 7.2 µg/L for the assay. Twenty-one-day exposures to 1.8 µg/ L and 3.6 µg/ L doses were conducted to evaluate the sub-lethal effects, and various toxicological endpoints were assessed on the 1, 7, 15 and 21 days of exposure. Acute toxic stress was observed with fish displaying behavioral toxicity. The most hematological change was extreme microcytic hypochromic anemia. Leucocyte count increased in experimented fish. Moderate neutrophilia, monocytosis and lymphocytosis were observed. Serum total protein, albumin, and globulin concentrations were significantly diminished. Overall, increments over control were recognized in serum urea, creatinine and acid phosphatase. However, serum glucose, total lipid, cholesterol, serum ALT and AST activity showed a significant decrease in fish exposed to both concentrations of PRO. Serum IgM concentrations insignificantly changed in treated fish except for on the 21 day of exposure to 3.6 µg/ L of PRO, while serum lysozyme significantly decreased. Furthermore, total protein, lipid and glycogen concentrations in muscles and the liver exhibited a decreasing trend at all concentrations. Moreover, histopathological alterations in the liver, kidney, and muscles occurred exclusively after treatment. From the obtained results, it is assumed that profenofos induced general toxic impacts under field conditions and might disturb ecologically relevant processes.
Topics: Animals; Insecticides; Carps; Organothiophosphates; Lipids; Fish Diseases; Diet; Animal Feed
PubMed: 36708414
DOI: 10.1007/s10646-023-02628-9 -
Frontiers in Toxicology 2022Organophosphorus pesticides (OPs) are a chemically diverse class of commonly used insecticides. Epidemiological studies suggest that low dose chronic prenatal and infant...
Organophosphorus pesticides (OPs) are a chemically diverse class of commonly used insecticides. Epidemiological studies suggest that low dose chronic prenatal and infant exposures can lead to life-long neurological damage and behavioral disorders. While inhibition of acetylcholinesterase (AChE) is the shared mechanism of acute OP neurotoxicity, OP-induced developmental neurotoxicity (DNT) can occur independently and/or in the absence of significant AChE inhibition, implying that OPs affect alternative targets. Moreover, different OPs can cause different adverse outcomes, suggesting that different OPs act through different mechanisms. These findings emphasize the importance of comparative studies of OP toxicity. Freshwater planarians are an invertebrate system that uniquely allows for automated, rapid and inexpensive testing of adult and developing organisms in parallel to differentiate neurotoxicity from DNT. Effects found only in regenerating planarians would be indicative of DNT, whereas shared effects may represent neurotoxicity. We leverage this unique feature of planarians to investigate potential differential effects of OPs on the adult and developing brain by performing a comparative screen to test 7 OPs (acephate, chlorpyrifos, dichlorvos, diazinon, malathion, parathion and profenofos) across 10 concentrations in quarter-log steps. Neurotoxicity was evaluated using a wide range of quantitative morphological and behavioral readouts. AChE activity was measured using an Ellman assay. The toxicological profiles of the 7 OPs differed across the OPs and between adult and regenerating planarians. Toxicological profiles were not correlated with levels of AChE inhibition. Twenty-two "mechanistic control compounds" known to target pathways suggested in the literature to be affected by OPs (cholinergic neurotransmission, serotonin neurotransmission, endocannabinoid system, cytoskeleton, adenyl cyclase and oxidative stress) and 2 negative controls were also screened. When compared with the mechanistic control compounds, the phenotypic profiles of the different OPs separated into distinct clusters. The phenotypic profiles of adult vs. regenerating planarians exposed to the OPs clustered differently, suggesting some developmental-specific mechanisms. These results further support findings in other systems that OPs cause different adverse outcomes in the (developing) brain and build the foundation for future comparative studies focused on delineating the mechanisms of OP neurotoxicity in planarians.
PubMed: 36267428
DOI: 10.3389/ftox.2022.948455 -
Scientific Reports Oct 2022Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) an invasive mealybug on cotton is primarily controlled by conventional insecticides. An endoparasitoid...
Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) an invasive mealybug on cotton is primarily controlled by conventional insecticides. An endoparasitoid Aenasius arizonenesis (Girault) (Hymenoptera: Encyrtidae) is a potential biocontrol agent of this pest. We assessed the susceptibility in field populations of P. solenopsis and A. arizonensis to commonly used insecticides: profenofos, imidacloprid and thiodicarb. Reproductive traits of the parasitoid and Environmental Risk Assessment (ERA) parameters viz., Reduction coefficient, Descriptive analysis, Risk Index (RI), Selectivity ratio and Hazard quotient were measured to assess the direct and indirect effects of these insecticides on the parasitoid. Probit analysis revealed heterogeneity in the insecticide resistance development for both the cotton mealybug and its parasitoid. The field populations of P. solenopsis exhibited resistance to profenofos (18.87-59.86 folds) and thiodicarb (20.07 folds) and susceptibility to imidacloprid. Development of resistance to profenofos was observed in field populations of A. arizonensis. Exposure to lethal doses of imidacloprid and profenofos caused a reduction in parasitization (19-23%) and adult emergence (62-69%) of the parasitoid. Profenofos, thiodicarb and imidacloprid were found to be hazardous, non-selective and harmful to the endoparasitoid, A. arizonensis. There is an urgent need for optimizing insecticide applications for sustainable management of this invasive mealybug in cotton.
Topics: Animals; Gossypium; Hemiptera; Hymenoptera; Insecticide Resistance; Insecticides; Neonicotinoids; Nitro Compounds; Organothiophosphates; Thiocarbamates
PubMed: 36202878
DOI: 10.1038/s41598-022-20779-3 -
Toxics Jul 2022In Thailand, pesticides containing organophosphates (OP) are frequently applied to crops to suppress insects. School children can be exposed to OPs on a daily basis,...
The Assessment of Organophosphate Pesticide Exposure among School Children in Four Regions of Thailand: Analysis of Dialkyl Phosphate Metabolites in Students' Urine and Organophosphate Pesticide Residues in Vegetables for School Lunch.
In Thailand, pesticides containing organophosphates (OP) are frequently applied to crops to suppress insects. School children can be exposed to OPs on a daily basis, from food consumption to breathing and touching pesticides drifted near classrooms. Living in an agricultural area can also be one of the causes. As a result, it is important to monitor OPs residues in the food chain and biomarkers of exposure. The Gas Chromatography-Flame Photometric Detector method was employed to examine the relationship between OPs residue and DAPs (Diakly phosphate) in four targeted locations in Thailand, as well as to examine the residues of OPs in vegetable samples and DAPs in 395 school children's urine samples. Vegetables were found to contain at least one OP, with chlorpyrifos being the most prevalent. The OPs detected frequencies for Sakon Nakhon, Chiang Mai, Phang Nga, and Pathum Thani are 96.1%, 94%, 91.7%, and 83.3%, respectively. The overall centration level of OPs showed 0.3261 mg/kg, 0.0636 mg/kg, 0.0023 mg/kg, 0.0150 mg/kg, 0.2003 mg/kg, 0.0295 mg/kg, and 0.0034 mg/kg for diazinon, dimethoate, pirimiphos-methyl, chlorpyrifos, profenofos, ethion, and triazophos, respectively. Nearly 98% of school children were detected with at least one DAP. The overall level of dimethyl phosphate metabolites (5.258 µmole/mole creatinine) in urine samples is higher than diethyl phosphate metabolites (2.884 µmole/mole creatinine), especially in the case of Pathum Thani. Our findings show a consistent relationship between OPs in vegetables from wet markets and DAPs in urine samples of school children in various parts of Thailand.
PubMed: 36006115
DOI: 10.3390/toxics10080434