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PloS One 2024Women living with HIV (WLWH) have high risk of developing cervical cancer. High- risk Human papillomavirus (hrHPV) is the single most important cause of cervical cancer....
BACKGROUND
Women living with HIV (WLWH) have high risk of developing cervical cancer. High- risk Human papillomavirus (hrHPV) is the single most important cause of cervical cancer. Vaccination for and early detection of pre-malignant cervical changes, through cervical cancer screening contributes to prevention of cervical cancer. This study sought to determine the prevalence of HPV among WLWH, genotypes present and the risk factors associated with cervical cancer development.
METHODS AND FINDINGS
An analytical cross-sectional study of 250 sexually active women aged 18 years and above, attending HIV clinic at a tertiary health facility in Accra. Demographic data collection and risk factor assessments were done using interviewer-administered questionnaire, and patient records. Cervical swabs were collected and tested for HPV using real-time PCR assays. Genotype analysis was performed on 92 samples. Descriptive statistics and logistic regression analysis were used to establish associations between hrHPV and risk factors among WLWH. Approximately 60% of study participants tested positive for HPV. The prevalence of hr-HPV among WLH was 44.4%. Factors identified to be protective of hrHPV were employment (AOR = 0.19, 95% CI = 0.06, 0.56, p = 0.003) and highly active antiretroviral therapy (HAART) Tenofovir-Lamivudine-Ritonavir-Lopinavir (TLRL) (AOR = 0.30, 95% CI = 0.09, 0.95, p = 0.04). Women with HIV diagnosis within 6 to10 years (AOR = 4.89, 95% CI = 1.05, 22.70, p = 0.043) and diagnosis >10 years (AOR = 8.25, 95% CI = 1.24, 54.84, p = 0.029) had higher odds of hrHPV. Approximately 25% of samples analysed tested positive for hr-HPV group 1 (genotypes 16, 18, 31, 33, 35, 39, 45,51, 52, 56, 58, 69) and 46.8% for multiple HPV genotypes.
CONCLUSION
A high prevalence of genotypes that include high risk genotypes 16 and 18 and multiple HPV infections was found among WLWH. Almost half of the women screened had high-risk HPV and were prone to cervical cancer without their knowledge. Regular HPV screening is recommended for high-risk patient groups.
Topics: Humans; Female; Adult; Papillomavirus Infections; HIV Infections; Risk Factors; Prevalence; Ghana; Cross-Sectional Studies; Middle Aged; Young Adult; Uterine Cervical Neoplasms; Adolescent; Papillomaviridae; Tertiary Care Centers
PubMed: 38814956
DOI: 10.1371/journal.pone.0303535 -
F1000Research 2023Two-wheeled vehicles are the major mode of transportation in India. Such vehicles are exposed to excessive vibration on the road when compared to four-wheeled vehicles....
BACKGROUND
Two-wheeled vehicles are the major mode of transportation in India. Such vehicles are exposed to excessive vibration on the road when compared to four-wheeled vehicles. However, the research on the reduction of whole body vibration in the case of two-wheelers is not explored in detail. The present study predicts rider comfort in the case of an electric two-wheeler as per ISO 2631-1, by obtaining the finding the weighted acceleration at the strategic locations of vibration at the test rig.
METHODS
An electric two-wheeler test rig is used in the study. The values of acceleration from the test rig in running conditions are obtained by using NI LabVIEW 2019. The drive cycle of the electric vehicle (EV) test rig is controlled by Sync sols' EV lab software. Obtaining the weighted root mean square (RMS) acceleration from running the test setup, it is compared with the ISO 2631-1 standard to obtain the rider comfort.
RESULTS
Loading area, traction motor, base mount, and suspension were found to be the strategic points of vibration. Frequency weighted RMS acceleration of 0.3 to 0.4 m/s obtained at these points are prone to cause discomfort for the rider. Vehicle speed, road profile, and duration of exposure were found to be important parameters affecting the rider's comfort. A maximum of 4.6 m/s amplitude was observed. The loading area, which corresponds to a rider's seat in actual vehicle, is important and reduction of these vibrations make the ride comfortable for the rider. Suspension and base mount of the test rig are found to be uncomfortable observing the weighted RMS acceleration.
CONCLUSIONS
A suitable damping technique design is very much essential in reducing these vibrations and improve the rider comfort, as many more non-deterministic vibrations are prone to cause dis-comfort in case of actual on road riding conditions.
Topics: Vibration; Humans; Acceleration; Motor Vehicles; Electricity
PubMed: 38813349
DOI: 10.12688/f1000research.131105.3 -
Heliyon May 2024Lead-acid batteries are noted for simple maintenance, long lifespan, stable quality, and high reliability, widely used in the field of energy storage. However, during...
Lead-acid batteries are noted for simple maintenance, long lifespan, stable quality, and high reliability, widely used in the field of energy storage. However, during the use of lead-acid batteries, the negative electrode is prone to irreversible sulfation, failing to meet the requirements of new applications such as maintenance-free hybrid vehicles and solar energy storage. In this study, in order to overcome the sulfation problem and improve the cycle life of lead-acid batteries, active carbon (AC) was selected as a foaming agent and foam fixing agent, and carbon foams (CF) with layered porous structure was prepared by mixing with molten sucrose. Sucrose as raw material is green and cheap, and the material preparation process is simple. The prepared CF material was then added as an additive to the negative electrode plate, and the electrochemical performance of the electrode plate and the battery was studied. The results proved that the addition of CF could effectively inhibit the sulfate formation of the negative electrode plate, with the 1.0 % CF negative electrode plate showing the best electrochemical performance. Specifically, according to the result of battery cycle testing, the simulated battery with CF had a cycle life of 3642 times, which was 2.87 times that of the blank group and 2.39 times of the AC group. Meanwhile, rate testing showed that the simulated battery with CF could maintain a high capacity even under high-rate discharge conditions.
PubMed: 38813151
DOI: 10.1016/j.heliyon.2024.e31339 -
Journal of Surgical Case Reports May 2024Gingival enlargement is a side effect of several different medication, including immunosuppressants, anticonvulsants, and calcium channel blockers. It is an inflammatory...
Gingival enlargement is a side effect of several different medication, including immunosuppressants, anticonvulsants, and calcium channel blockers. It is an inflammatory response that starts when plaque and calculus build up on the tooth surface. The most prevalent long-term neurological condition affecting people is epilepsy. In affluent nations, the prevalence of epilepsy is ~ 1%, whereas in less developed countries, it may >2%. The preferred medication for the condition, phenytoin, has major side effects include gingival enlargement. In addition to being visually disfiguring, this enlargement frequently affects speech, chewing and eating. Furthermore, those with poor dental hygiene, causes disabilities with motor coordination and muscular limitations leading to mental disability and physical impairments are more prone to periodontal disease. This article enlightened the mechanism of drug induced gingival enlargement clinically, microbiologically, and surgically.
PubMed: 38812578
DOI: 10.1093/jscr/rjae304 -
BMC Plant Biology May 2024Salinity stress, an ever-present challenge in agriculture and environmental sciences, poses a formidable hurdle for plant growth and productivity in saline-prone regions...
Salinity stress, an ever-present challenge in agriculture and environmental sciences, poses a formidable hurdle for plant growth and productivity in saline-prone regions worldwide. Therefore, this study aimed to explore the effectiveness of trehalose and mannitol induce salt resistance in wheat seedlings. Wheat grains of the commercial variety Sakha 94 were divided into three groups : a group that was pre-soaked in 10 mM trehalose, another group was soaked in 10 mM mannitol, and the last was soaked in distilled water for 1 hour, then the pre soaked grains cultivated in sandy soil, each treatment was divided into two groups, one of which was irrigated with 150 mM NaCl and the other was irrigated with tap water. The results showed that phenols content in wheat seedlings increased and flavonoids reduced due to salt stress. Trehalose and mannitol cause slight increase in total phenols content while total flavonoids were elevated highy in salt-stressed seedlings. Furthermore, Trehalose or mannitol reduced salt-induced lipid peroxidation. Salt stress increases antioxidant enzyme activities of guaiacol peroxidase (G-POX), ascorbate peroxidase (APX), and catalase (CAT) in wheat seedlings, while polyphenol oxidase (PPO) unchanged. Trehalose and mannitol treatments caused an increase in APX, and CAT activities, whereas G-POX not altered but PPO activity were decreased under salt stress conditions. Molecular docking confirmed the interaction of Trehalose or mannitol with peroxidase and ascorbic peroxidase enzymes. Phenyl alanine ammonia layase (PAL) activity was increased in salt-stressed seedlings. We can conclude that pre-soaking of wheat grains in 10 mM trehalose or mannitol improves salinity stress tolerance by enhancing antioxidant defense enzyme and/or phenol biosynthesis, with docking identifying interactions with G-POX, CAT, APX, and PPO.
Topics: Triticum; Trehalose; Seedlings; Mannitol; Salt Tolerance; Molecular Docking Simulation; Antioxidants; Salt Stress; Flavonoids; Phenols
PubMed: 38811894
DOI: 10.1186/s12870-024-04964-2 -
Scientific Reports May 2024Electric motors are essential equipment widely employed in various sectors. However, factors such as prolonged operation, environmental conditions, and inadequate...
Electric motors are essential equipment widely employed in various sectors. However, factors such as prolonged operation, environmental conditions, and inadequate maintenance make electric motors prone to various failures. In this study, we propose a thermography-based motor fault detection method based on InceptionV3 model. To enhance the detection accuracy, we apply Contrast Limited Adaptive Histogram Equalization (CLAHE) to the input images. Furthermore, we improved the performance of the InceptionV3 by integrating a Squeeze-and-Excitation (SE) channel attention mechanism. The proposed model was tested using a dataset containing 369 thermal images of an electric motor with 11 types of faults. Image augmentation was employed to increase the data size and the evaluation was conducted using fivefold cross validation. Experimental results indicate that the proposed model can achieve accuracy, precision, recall, and F1 score of 98.82%, 98.93%, 98.82%, and 98.87%, respectively. Additionally, by freezing the fully connected layers of the InceptionV3 model for feature extraction and training a Support Vector Machines (SVM) to perform classification, it is able to achieve 100% detection rate across all four evaluation metrics. This research contributes to the field of industrial motor fault diagnosis. By incorporating deep learning techniques based on InceptionV3 and SE channel attention mechanism with a traditional classifier, the proposed method can accurately classify different motor faults.
PubMed: 38811686
DOI: 10.1038/s41598-024-63086-9 -
Scientific Reports May 2024Atherosclerosis is the build-up of fatty plaques within blood vessel walls, which can occlude the vessels and cause strokes or heart attacks. It gives rise to both...
Atherosclerosis is the build-up of fatty plaques within blood vessel walls, which can occlude the vessels and cause strokes or heart attacks. It gives rise to both structural and biomolecular changes in the vessel walls. Current single-modality imaging techniques each measure one of these two aspects but fail to provide insight into the combined changes. To address this, our team has developed a dual-modality imaging system which combines optical coherence tomography (OCT) and fluorescence imaging that is optimized for a porphyrin lipid nanoparticle that emits fluorescence and targets atherosclerotic plaques. Atherosclerosis-prone apolipoprotein (Apo)e mice were fed a high cholesterol diet to promote plaque development in descending thoracic aortas. Following infusion of porphyrin lipid nanoparticles in atherosclerotic mice, the fiber-optic probe was inserted into the aorta for imaging, and we were able to robustly detect a porphyrin lipid-specific fluorescence signal that was not present in saline-infused control mice. We observed that the nanoparticle fluorescence colocalized in areas of CD68 macrophages. These results demonstrate that our system can detect the fluorescence from nanoparticles, providing complementary biological information to the structural information obtained from simultaneously acquired OCT.
Topics: Tomography, Optical Coherence; Animals; Plaque, Atherosclerotic; Nanoparticles; Mice; Porphyrins; Optical Imaging; Disease Models, Animal; Atherosclerosis; Macrophages; Lipoproteins, HDL
PubMed: 38811670
DOI: 10.1038/s41598-024-63132-6 -
Journal of Research in Medical Sciences... 2024Type 2 diabetes mellitus (T2DM) is prone to opportunistic infections, including toxoplasmosis, due to an immunodeficiency system. This study aimed to evaluate the serum...
BACKGROUND
Type 2 diabetes mellitus (T2DM) is prone to opportunistic infections, including toxoplasmosis, due to an immunodeficiency system. This study aimed to evaluate the serum of people with T2DM to determine the titer of anti-toxoplasma antibodies in patients and compare it with the control group.
MATERIALS AND METHODS
720 blood samples have been carried out between October and the end of January 2017 in Sistan, and Baluchestan provinces in southeastern Iran, of these, 360 samples were related to healthy individuals (control), and 360 samples were related to T2DM individuals. The immunoglobulin (Ig) M and IgG enzyme-linked immunosorbent assay methods have been used to detect toxoplasmosis. The data were analyzed using SPSS-19, Chi-square, and Fisher's exact test to compare statistical parameters.
RESULTS
In this cross-sectional study, out of 360 samples of T2DM by ELISA method, 60% samples in diabetic patients and 48.1% in control group were IgG positive ( < 0.05). Nearly 2.5% samples in diabetic patients and 0.3% in control group were IgM positive ( < 0.05).
CONCLUSION
Anti-toxoplasma antibodies including IgG and IgM were higher in diabetic patient in comparison to control group.
PubMed: 38808216
DOI: 10.4103/jrms.jrms_49_23 -
Frontiers in Cellular and Infection... 2024is a ubiquitous protozoon in the intestinal tract of human and many animals. Microscopic examination is the main method of clinical diagnosis for , which is prone to...
is a ubiquitous protozoon in the intestinal tract of human and many animals. Microscopic examination is the main method of clinical diagnosis for , which is prone to false negative. A simple and rapid diagnosis of infection is an important step to prevent and control blastocystosis. Here, a recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) assay was developed for rapid visual detection of DNA amplification could be performed within 18 min at 37°C. The minimum DNA detection limit was 1 pg/μL, and there was no cross-reactivity with 12 other non-target pathogens, which was consistent with the sensitivity of conventional PCR (cPCR). Furthermore, 56 fecal samples from the Third Affiliated Hospital of Xinxiang Medical University were tested using RPA and cPCR methods respectively, and the results were completely consistent. The results show that RPA-LFD method has high accuracy and visual results, which provides a new choice for the differential diagnosis and rapid field detection of
Topics: Blastocystis; Humans; Blastocystis Infections; Nucleic Acid Amplification Techniques; Sensitivity and Specificity; Feces; Molecular Diagnostic Techniques; DNA, Protozoan; Recombinases
PubMed: 38808061
DOI: 10.3389/fcimb.2024.1391943 -
Frontiers in Public Health 2024Work-related musculoskeletal disorders (WMSDs) are considered major public health problems globally, deteriorating the quality of life of workers in various occupations....
Work-related musculoskeletal disorders: prevalence, associated factors, and impact on quality of life among kitchen workers in hospitality industry, Bahir Dar City, Northwest Ethiopia, 2023.
INTRODUCTION
Work-related musculoskeletal disorders (WMSDs) are considered major public health problems globally, deteriorating the quality of life of workers in various occupations. Kitchen work is reported as among the occupations most prone to these maladies. Nevertheless, prevalence of WMSDs, contributing factors, and impacts on the quality of life of hospitality industry kitchen workers are insufficiently documented in Ethiopia. Therefore, this study aimed to assess the prevalence of WMSDs, their associated factors, and impact on the quality of life of hospitality industry kitchen workers in Bahir Dar city, Ethiopia.
METHODS
An institution-based, cross-sectional study was conducted from 17 April to 17 May 2023. A total of 422 participants were included using a simple random sampling technique. WMSDs were evaluated using an interviewer-administered Nordic standardized questionnaire. The short form-36 questionnaire was used to assess quality of life. The data were collected using the Kobo tool box. SPSS version 26 software was used to perform both bivariable and multivariable binary logistic regression analyses. Independent -tests were used to show the impact of WMSDs on quality of life scales across groups with and without WMSD symptoms.
RESULT
In this study, the response rate was 98.34% ( = 415). The 1-year prevalence of WMSDs among kitchen workers was 82.7% [95% CI: (79.1, 86.3)]. Age group between 30 and 39 years [AOR: 2.81; 95% CI: (1.46-5.41)], job dissatisfaction [AOR: 2.45; 95% CI: (1.34-4.45)], anxiety [AOR: 2.26; 95% CI: (1.12-4.52)], prolonged standing [AOR: 3.81; 95% CI: (1.58-9.17)], and arm overreaching [AOR: 2.43; 95% CI: (1.34-4.41)] were significantly associated factors with work-related musculoskeletal disorders. Work-related musculoskeletal disorders had a significant impact on all quality of life dimensions, in which the mean SF-36 scores of participants with WMSDs were lower than those of their non-WMSD counterparts.
CONCLUSION
This study revealed that the prevalence of WMSDs was relatively high. Age between 30 and 39 years, job dissatisfaction, anxiety, prolonged standing, and arm overreaching were identified as significant determinants of WMSDs among kitchen workers in hospitality industries. The presence of one or multiple WMSDs, in turn, is associated with worse quality of life dimensions of individuals.
Topics: Humans; Ethiopia; Quality of Life; Adult; Male; Musculoskeletal Diseases; Cross-Sectional Studies; Female; Prevalence; Occupational Diseases; Surveys and Questionnaires; Middle Aged; Risk Factors; Young Adult; Cooking
PubMed: 38807998
DOI: 10.3389/fpubh.2024.1358867