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IDCases 2023, formerly known as , is an infrequently encountered aerobic gram-negative bacterium. While it has been identified as a potential human bacterial pathogen, its...
, formerly known as , is an infrequently encountered aerobic gram-negative bacterium. While it has been identified as a potential human bacterial pathogen, its connection to specific clinical conditions remains limited. Here, we present an exceptional case of a 27-year-old immunocompetent man with acute tonsillitis, who developed bacteremia. This unique correlation, not extensively documented in previous studies, sheds light on the potential pathogenicity of in patients with acute tonsillitis.
PubMed: 37701050
DOI: 10.1016/j.idcr.2023.e01891 -
Cureus May 2023Purpose Multidrug-resistant (MDR) organisms are being increasingly reported from India. This study aimed to determine the antibiotic susceptibility pattern of...
Emergence of Multidrug-Resistant Non-Fermenting Gram-Negative Bacilli in a Tertiary Care Teaching Hospital of Central India: Is Colistin Resistance Still a Distant Threat?
Purpose Multidrug-resistant (MDR) organisms are being increasingly reported from India. This study aimed to determine the antibiotic susceptibility pattern of non-fermenting Gram-negative bacilli (NF-GNB) isolated from all the clinical samples to estimate the prevalence of MDR MDR NF-GNB and to screen for colistin-resistance genes among all colistin-resistant strains. Materials and methods This prospective study conducted from January 2021 to July 2022 at a tertiary care teaching hospital in central India identified MDR NF-GNB from clinical samples using standard procedures and antimicrobial susceptibility testing conducted as per Clinical Laboratory Standards Institute (CLSI) guidelines. Colistin-resistant strains identified by broth microdilution were further subjected to detection of plasmid-mediated colistin-resistant genes (, , ) by polymerase chain reaction (PCR). Results A total 2,106 NF-GNB were isolated from 21,019 culture positive clinical samples, of which 743 (35%) were MDR. Majority of MDR NF-GNB isolated were from pus (45.50%) followed by blood (20.50%). Out of 743 non-duplicate MDR non-fermenters,the most common were (51.7%) (23.4%),and others (24.9%).Around5.2% and 2.3% were resistant to colistin, and 88.2% were resistant to ceftazidime. complexwas 100% susceptible to minocycline and least susceptible to ceftazidime (28.6%). Out of 11, 10 (90.9%) were susceptible to colistin and least susceptible to ceftazidime and minocycline (27.3%). All 33 colistin-resistant strains (minimal inhibitory concentration ≥ 4 µg/mL) were found to be negative for , , and genes. Conclusion Our study showed a significantly wide variety of NF-GNB, ranging from (51.7%), (23.4%),to (4.6%), (0.9%), (0.7%), (0.5%), and (0.4%), which have not been commonly reported in literature. Of all the non-fermenters isolated in the present study, 35.28% were MDR, raising the concern for rationalizing antibiotic use and improving infection control measures to avert or slow the emergence of antibiotic resistance.
PubMed: 37342731
DOI: 10.7759/cureus.39243 -
Journal of Food Science and Technology Dec 2022The antimicrobial properties of two different emulsion formulations contained lemon essential oil (coarse emulsion; CE and nanoemulsion; NE) and emulsions based edible...
The antimicrobial properties of two different emulsion formulations contained lemon essential oil (coarse emulsion; CE and nanoemulsion; NE) and emulsions based edible solutions incorporated with lemon essential oil and sodium caseinate (coarse emulsion based solution; CESC and nanoemulsion based solution; NESC) on food-related microorganisms (, , Paratyphi A NCTC13, and ATCC19112) were investigated. The chemical compositions of lemon essential oils were identified by GC-MS. Physical parameter of different formulations was also analyzed at different time intervals. The antimicrobial properties of solutions were determined by using well diffusion, minimum inhibitory concentration (MIC) and minimum bactericidal concentration, and time-kill assay. The major identified component in lemon essential oil was D limonene (38.38%). NE showed a stronger antimicrobial effect against Paratyphi A and with 36.50 and 38.75 mm diameter zone compared to all other formulations. had the highest sensitivity towards NE and NESC formulations with 3.12 mg/ml MIC values in comparison to other two formulations. The nanoemulsion and nanoemulsion based coating solution were more effective than other formulations in killing bacterial cell within a short period time.
PubMed: 36276524
DOI: 10.1007/s13197-022-05551-9 -
The American Journal of Case Reports Apr 2022BACKGROUND Pseudomonas luteola (P. luteola) is a Gram-negative, oxidase-negative is an environmental organism that is isolated from soil, water, and damping areas, and... (Review)
Review
BACKGROUND Pseudomonas luteola (P. luteola) is a Gram-negative, oxidase-negative is an environmental organism that is isolated from soil, water, and damping areas, and is rarely found as a human pathogen. Most infections in which P. luteola is implicated are associated with a breach in immune barrier, such as indwelling catheters, prosthetic devices, immunocompromised conditions and surgical wounds. CASE REPORT A 9 years old girl, known case of Chron's disease and recurrent urinary tract infections on prophylactic antibiotics, presented with an acute-onset abdominal pain associated with fever reaching 40C and vomiting. She was placed on a peripherally inserted central line for total parenteral nutrition and developed sepsis on the 30th day of admission. Septic workup revealed P. letuola infection with right atrial vegetation. Treatment with Piperacillin/tazobactam yielded an uneventful recovery. CONCLUSIONS P. luteola can be involved in serious infections in susceptible individuals. Serious outcomes may be associated with infective endocarditis especially on a background of valvular prosthesis and central lines. The definitive treatment of catheter related infective endocarditis caused by P. leuteola is the removal of the lines along with an appropriate antibiotic regimen-based AST result.
Topics: Central Venous Catheters; Child; Endocarditis; Endocarditis, Bacterial; Female; Humans; Pseudomonas
PubMed: 35365592
DOI: 10.12659/AJCR.935743 -
Microbiology Resource Announcements Feb 2022This is a report of genome characterization of Pseudomonas phage AIIMS-Plu-RaNi infecting Pseudomonas luteola. The phage belonged to the family Siphoviridae with...
This is a report of genome characterization of Pseudomonas phage AIIMS-Plu-RaNi infecting Pseudomonas luteola. The phage belonged to the family Siphoviridae with icosahedral head and tail with a genome of 46.6 kb, 64.45% GC with 68 open reading frames.
PubMed: 35175114
DOI: 10.1128/mra.01113-21 -
Case Reports in Infectious Diseases 2021is rarely considered as a human pathogen.There are only fewer than twenty reported cases of infections since 1950. It has been described in both immunocompromised and...
is rarely considered as a human pathogen.There are only fewer than twenty reported cases of infections since 1950. It has been described in both immunocompromised and immunocompetent patients as a cause of both nosocomial and community-acquired infections. We report a rare case of infection in a previously healthy patient who was admitted to hospital with a first presentation of Systemic Lupus Erythematosus (SLE) presenting with bacteremia.
PubMed: 34434585
DOI: 10.1155/2021/4051378 -
Journal of Environmental and Public... 2021Gram-negative bacilli can spread from the environment and through food products. This study aimed to characterize ESBL production and virulence genes from...
Gram-negative bacilli can spread from the environment and through food products. This study aimed to characterize ESBL production and virulence genes from multidrug-resistant Gram-negative bacilli isolated from specimen collected from the environment, kitchen, and food products. A total of 130 samples were collected at local markets in seven different communities in Benin (Abomey-Calavi, Ouidah, Bohicon, Abomey, Parakou, Djougou, and Grand-Popo). Samples were cultured on McConkey and ChromID ESBL agar plates. The isolates were identified by the API 20E gallery. An antibiotic susceptibility test was carried out, and the detection of ESBL production and virulence-associated genes was carried out by Polymerase Chain Reaction (PCR). The data collected was coded and analyzed using GraphPad prism 7 software and Excel. The software R was used to calculate the correlation coefficient between the results of the detection of ESBL+ on agar and by the effect of the double synergy. The results showed that sixty-three (63) bacterial strains were isolated from the 130 samples, of which the dominant species was (10/63). The kitchen samples were the most contaminated with 36.50%. More than 40% of the isolates were resistant to at least three different classes of antibiotics. Also, blaSHV gene was detected in 33.33% (21/63) of the isolates and in all isolates of (5/5%). 11.11% (7/63) of isolates were virulent with dominance of the H gene, especially with (83.33%). The kitchen samples showed a high prevalence of ESBL-producing strains with H gene. This raises the problem of non-compliance with hygiene rules in community cooking and food handling.
Topics: Anti-Bacterial Agents; Benin; Drug Resistance; Environmental Microbiology; Escherichia coli; Food Microbiology; Humans; Pseudomonas
PubMed: 33613674
DOI: 10.1155/2021/8420590 -
Journal of Food Protection Jan 2021Kitfo is a version of beef tartar widely consumed in the Ethiopian community. It is made from raw minced beef and a blend of powdered spice and butter. Although previous...
ABSTRACT
Kitfo is a version of beef tartar widely consumed in the Ethiopian community. It is made from raw minced beef and a blend of powdered spice and butter. Although previous studies have shown that kitfo contains several bacteria that are of public health concern, the status of their antibiotic resistance is not known. In this study, the antibiotic resistance of bacterial isolates from 26 retail kitfo samples obtained from the Washington metropolitan area was analyzed. Characterization and antibiotic sensitivity of the isolates were determined by the Vitek 2 system and pulsed-field gel electrophoresis was used to delineate the intraspecies variations. Of the isolates, 59% were resistant to two or more antibiotics. Acinetobacter calcoaceticus and Pseudomonas luteola were multidrug resistant to the classes of β-lactam, cephalosporins, and nitrofurantoin. The antibiotic susceptibility profile of the isolates was cefazolin (59%), cefoxitin (50%), ampicillin (32%), and nitrofuran (18%). Most isolates (75%) were Enterobacteriaceae, whereas only 3.8 and 2.6% were Pseudomonadaceae and Moraxellaceae, respectively. Of the Enterobacteriaceae, Enterobacter cloacae, Escherichia coli, and Klebsiella spp. were the most predominant. All isolates except Klebsiella spp. showed high genetic variation (>65%). This study implicates for the first time kitfo as a potential reservoir of antibiotic-resistant bacteria.
Topics: Animals; Anti-Bacterial Agents; Cattle; Dental Calculus; Drug Resistance, Bacterial; Drug Resistance, Microbial; Microbial Sensitivity Tests; Prevalence; Pseudomonas; Washington; beta-Lactamases
PubMed: 33411928
DOI: 10.4315/JFP-20-230 -
International Journal of Molecular... Apr 2019The aim of the study was to demonstrate the potential of the promotion and regulation of plant physiology and growth under control and copper stress conditions, and the...
Bacterial Isolate Inhabiting Spitsbergen Soil Modifies the Physiological Response of in Control Conditions and under Exogenous Application of Methyl Jasmonate and Copper Excess.
The aim of the study was to demonstrate the potential of the promotion and regulation of plant physiology and growth under control and copper stress conditions, and the impact of the exogenous application of methyl jasmonate on this potential. Runner bean plants were treated with methyl jasmonate (1 or 10 µM) (J; J1 or J10) and Cu (50 µM), and inoculated with a bacterial isolate (S17) originating from Spitsbergen soil, and identified as using the analytical profile index (API) test. Above- and under-ground plant parts were analyzed. The growth parameters; the concentration of the photosynthetic pigments, elements, flavonoids (FLAVO), phenolics (TPC), allantoin (ALLA), and low molecular weight organic acids (LMWOAs); the activity of antioxidant enzymes and enzymes of resistance induction pathways (e.g., superoxide dismutase (SOD), catalase (CAT), ascorbate (APX) and guaiacol (GPX) peroxidase, glucanase (GLU), and phenylalanine (PAL) and tyrosine ammonia-lyase (TAL)), and the antioxidant capacity (AC) were studied. The leaves exhibited substantially higher ALLA and LMWOA concentrations as well as PAL and TAL activities, whereas the roots mostly had higher activities for a majority of the enzymes tested (i.e., SOD, CAT, APX, GPX, and GLU). The inoculation with S17 mitigated the effect of the Cu stress. Under the Cu stress and in the presence of J10, isolate S17 caused an elevation of the shoot fresh weight, K concentration, and TAL activity in the leaves, and APX and GPX (also at J1) activities in the roots. In the absence of Cu, isolate S17 increased the root length and the shoot-to-root ratio, but without statistical significance. In these conditions, S17 contributed to a 236% and 34% enhancement of P and Mn, respectively, in the roots, and a 19% rise of N in the leaves. Under the Cu stress, S17 caused a significant increase in FLAVO and TPC in the leaves. Similarly, the levels of FLAVO, TPC, and AC were enhanced after inoculation with Cu and J1. Regardless of the presence of J, inoculation at Cu excess caused a reduction of SOD and CAT activities, and an elevation of GPX. The effects of inoculation were associated with the application of Cu and J, which modified plant response mainly in a concentration-dependent manner (e.g., PAL, TAL, and LMWOA levels). The conducted studies demonstrated the potential for isolate S17 in the promotion of plant growth.
Topics: Acetates; Allantoin; Ammonia-Lyases; Copper; Cyclopentanes; Oxylipins; Peroxidase; Phaseolus; Photosynthesis; Plant Growth Regulators; Plant Proteins; Soil Microbiology; Superoxide Dismutase
PubMed: 30999692
DOI: 10.3390/ijms20081909 -
Veterinary World Jan 2019Rats are accused in disseminating many zoonotic diseases. This study aimed to isolate and identify bacteria from internal organs of rats captured in Baghdad City, Iraq.
AIM
Rats are accused in disseminating many zoonotic diseases. This study aimed to isolate and identify bacteria from internal organs of rats captured in Baghdad City, Iraq.
MATERIALS AND METHODS
A total of 120 black rats () were trapped from different areas in Baghdad city. Rats were kept in individual plastic cages for 3 h before euthanizing. Deep pharyngeal swab, intestinal content, urine, and pieces of the liver and spleen, lung, kidney, and brain were obtained aseptically. The specimens were inoculated into peptone water and incubated at 37°C for 24 h for enrichment. A loopful of each specimen was then subcultured onto MacConkey Agar, Blood Agar, and Mannitol Salt Agar. CHROMagar O157 H7 and CHROMagar Listeria were used to detect 157:7 and spp., respectively. Biochemical tests on analytical profile index, microscopic examination, and commercial kit for latex agglutination test for serotyping O157:H7 were used.
RESULTS
Mixed bacterial isolates were recorded as 116, 52, 36, 28, 18, 6, and 4 from intestinal contents, deep pharyngeal, liver and spleen, urine, lung, brain, and kidney, respectively. Microorganisms included , , spp., spp., , , , , , spp., spp., , spp., spp., , , spp., spp., , , and spp. The highest bacterial prevalence (88; 73.33%) was recorded for , where 68 isolates were identified from the intestinal contents. Of these, four isolates were O157:H7.
CONCLUSION
Rats are important carriers and transmitters of a number of pathogens and can disseminate these microorganisms to humans and animals.
PubMed: 30936664
DOI: 10.14202/vetworld.2019.119-125