-
Japanese Journal of Infectious Diseases 2015Pseudomonas luteola has rarely been reported as a human pathogen. The clinical manifestations of P. luteola bacteremia and its susceptibility to antibiotics have not...
Pseudomonas luteola has rarely been reported as a human pathogen. The clinical manifestations of P. luteola bacteremia and its susceptibility to antibiotics have not been characterized. This retrospective study was conducted at a 382-bed tertiary care center in Turkey. During the 9-year study period, 7 patients (5 females and 2 males) were diagnosed with P. luteola bacteremia. Six of these patients had hospital-acquired bacteremia, whereas 1 patient had community-acquired P. luteola infection. All patients had monomicrobial bacteremia. Antimicrobial susceptibility testing revealed that all strains of P. luteola were sensitive to amikacin, gentamicin, trimethoprim-sulfamethoxazole, and meropenem, and that all strains were resistant to piperacillin-tazobactam, aztreonam, and colistin. In conclusion, we believe that P. luteola can cause both community- and hospital-acquired bacteremia. Amikacin, gentamicin, trimethoprim-sulfamethoxazole, and meropenem were effective against P. luteola in the present study.
Topics: Adolescent; Anti-Bacterial Agents; Bacteremia; Child; Child, Preschool; Female; Humans; Infant; Male; Microbial Sensitivity Tests; Pseudomonas; Pseudomonas Infections; Retrospective Studies; Tertiary Care Centers; Turkey
PubMed: 25420649
DOI: 10.7883/yoken.JJID.2014.051 -
The American Journal of Case Reports Apr 2022BACKGROUND Pseudomonas luteola (P. luteola) is a Gram-negative, oxidase-negative is an environmental organism that is isolated from soil, water, and damping areas, and... (Review)
Review
BACKGROUND Pseudomonas luteola (P. luteola) is a Gram-negative, oxidase-negative is an environmental organism that is isolated from soil, water, and damping areas, and is rarely found as a human pathogen. Most infections in which P. luteola is implicated are associated with a breach in immune barrier, such as indwelling catheters, prosthetic devices, immunocompromised conditions and surgical wounds. CASE REPORT A 9 years old girl, known case of Chron's disease and recurrent urinary tract infections on prophylactic antibiotics, presented with an acute-onset abdominal pain associated with fever reaching 40C and vomiting. She was placed on a peripherally inserted central line for total parenteral nutrition and developed sepsis on the 30th day of admission. Septic workup revealed P. letuola infection with right atrial vegetation. Treatment with Piperacillin/tazobactam yielded an uneventful recovery. CONCLUSIONS P. luteola can be involved in serious infections in susceptible individuals. Serious outcomes may be associated with infective endocarditis especially on a background of valvular prosthesis and central lines. The definitive treatment of catheter related infective endocarditis caused by P. leuteola is the removal of the lines along with an appropriate antibiotic regimen-based AST result.
Topics: Central Venous Catheters; Child; Endocarditis; Endocarditis, Bacterial; Female; Humans; Pseudomonas
PubMed: 35365592
DOI: 10.12659/AJCR.935743 -
Microbiology Resource Announcements Feb 2022This is a report of genome characterization of Pseudomonas phage AIIMS-Plu-RaNi infecting Pseudomonas luteola. The phage belonged to the family Siphoviridae with...
This is a report of genome characterization of Pseudomonas phage AIIMS-Plu-RaNi infecting Pseudomonas luteola. The phage belonged to the family Siphoviridae with icosahedral head and tail with a genome of 46.6 kb, 64.45% GC with 68 open reading frames.
PubMed: 35175114
DOI: 10.1128/mra.01113-21 -
Antimicrobial Agents and Chemotherapy Jan 2010Pseudomonas luteola (formerly classified as CDC group Ve-1 and named Chryseomonas luteola) is an unusual pathogen implicated in rare but serious infections in humans. A...
Pseudomonas luteola (formerly classified as CDC group Ve-1 and named Chryseomonas luteola) is an unusual pathogen implicated in rare but serious infections in humans. A novel beta-lactamase gene, bla(LUT-1), was cloned from the whole-cell DNA of the P. luteola clinical isolate LAM, which had a weak narrow-spectrum beta-lactam-resistant phenotype, and expressed in Escherichia coli. This gene encoded LUT-1, a 296-amino-acid Ambler class A beta-lactamase with a pI of 6 and a theoretical molecular mass of 28.9 kDa. The catalytic efficiency of this enzyme was higher for cephalothin, cefuroxime, and cefotaxime than for penicillins. It was found to be 49% to 59% identical to other Ambler class A beta-lactamases from Burkholderia sp. (PenA to PenL), Ralstonia eutropha (REUT), Citrobacter sedlakii (SED-1), Serratia fonticola (FONA and SFC-1), Klebsiella sp. (KPC and OXY), and CTX-M extended-spectrum beta-lactamases. No gene homologous to the regulatory ampR genes of class A beta-lactamases was found in the vicinity of the bla(LUT-1) gene. The entire bla(LUT-1) coding region was amplified by PCR and sequenced in five other genetically unrelated P. luteola strains (including the P. luteola type strain). A new variant of bla(LUT-1) was found for each strain. These genes (named bla(LUT-2) to bla(LUT-6)) had nucleotide sequences 98.1 to 99.5% identical to that of bla(LUT-1) and differing from this gene by two to four nonsynonymous single nucleotide polymorphisms. The bla(LUT) gene was located on a 700- to 800-kb chromosomal I-CeuI fragment, the precise size of this fragment depending on the P. luteola strain.
Topics: Amino Acid Sequence; Anti-Bacterial Agents; Catheters, Indwelling; Chromosomes, Bacterial; Cloning, Molecular; Electrophoresis, Gel, Pulsed-Field; Genes, Bacterial; Genetic Variation; Isoelectric Focusing; Kinetics; Microbial Sensitivity Tests; Molecular Sequence Data; Phylogeny; Plasmids; Pseudomonas; Pseudomonas Infections; Recombinant Proteins; Reverse Transcriptase Polymerase Chain Reaction; Substrate Specificity; beta-Lactamases
PubMed: 19884377
DOI: 10.1128/AAC.00427-09 -
Case Reports in Infectious Diseases 2021is rarely considered as a human pathogen.There are only fewer than twenty reported cases of infections since 1950. It has been described in both immunocompromised and...
is rarely considered as a human pathogen.There are only fewer than twenty reported cases of infections since 1950. It has been described in both immunocompromised and immunocompetent patients as a cause of both nosocomial and community-acquired infections. We report a rare case of infection in a previously healthy patient who was admitted to hospital with a first presentation of Systemic Lupus Erythematosus (SLE) presenting with bacteremia.
PubMed: 34434585
DOI: 10.1155/2021/4051378 -
Journal of Bacteriology Oct 2012Pseudomonas luteola XLDN4-9 and Pseudomonas stutzeri XLDN-R are two efficient carbazole-degrading pseudomonad strains. Here we present 4.63- and 4.70-Mb assemblies of...
Pseudomonas luteola XLDN4-9 and Pseudomonas stutzeri XLDN-R are two efficient carbazole-degrading pseudomonad strains. Here we present 4.63- and 4.70-Mb assemblies of their genomes. Their annotated key genes for carbazole catabolism are similar, which may provide further insights into the molecular mechanism of carbazole degradation in Pseudomonas.
Topics: Carbazoles; DNA, Bacterial; Genome, Bacterial; Metabolic Networks and Pathways; Molecular Sequence Data; Pseudomonas; Sequence Analysis, DNA
PubMed: 23012282
DOI: 10.1128/JB.01296-12 -
Journal of Comparative Pathology Jan 2012Between 2008 and 2009, three pet ferrets from different sources presented with acute episode of dyspnoea. Cytological examination of pleural exudates revealed severe...
Between 2008 and 2009, three pet ferrets from different sources presented with acute episode of dyspnoea. Cytological examination of pleural exudates revealed severe purulent inflammation with abundant clusters of rod-shaped microorganisms with a clear surrounding halo. Treatment was ineffective and the ferrets died 2-5 days later. Two ferrets were subjected to necropsy examination, which revealed pyothorax, mediastinal lymphadenopathy and multiple white nodules (1-2mm) in the lungs. Microscopical examination showed multifocal necrotizing-pyogranulomatous pleuropneumonia and lymphadenitis with aggregates of encapsulated microorganisms, some of which were positively stained by periodic acid-Schiff and alcian blue. In-situ hybridization for Pneumocystis spp., Ziehl-Neelsen staining and immunohistochemistry for distemper, coronavirus and influenza antigen were negative in all cases. Electron microscopically, the bacteria were 2-3 μm long with a thick electron-lucent capsule. Microbiology from one ferret yielded a pure culture of gram-negative bacteria identified phenotypically as Pseudomonas luteola. This speciation was later confirmed by 16S RNA gene amplification.
Topics: Animals; Ferrets; Mediastinitis; Pleuropneumonia; Pseudomonas; Pseudomonas Infections
PubMed: 21601873
DOI: 10.1016/j.jcpa.2011.03.014 -
Lipids in Health and Disease Jan 2014An amylase and lipase producing bacterium (strain C2) was enriched and isolated from soil regularly contaminated with olive washing wastewater in Sfax, Tunisia. Cell was...
An amylase and lipase producing bacterium (strain C2) was enriched and isolated from soil regularly contaminated with olive washing wastewater in Sfax, Tunisia. Cell was aerobic, mesophilic, Gram-negative, motile, non-sporulating bacterium, capable of growing optimally at pH 7 and 30°C and tolerated maximally 10% (W/V) NaCl. The predominant fatty acids were found to be C(18:1)ω7c (32.8%), C(16:1)ω7c (27.3%) and C16:0 (23.1%). Phylogenetic analysis of the 16S rRNA gene revealed that this strain belonging to the genus Pseudomonas. Strain C2 was found to be closely related to Pseudomonas luteola with more than 99% of similarity. Amylase optimization extraction was carried out using Box Behnken Design (BBD). Its maximal activity was found when the pH and temperature ranged from 5.5 to 6.5 and from 33 to 37°C, respectively. Under these conditions, amylase activity was found to be about 9.48 U/ml.
Topics: Amylases; Anti-Bacterial Agents; Bacterial Proteins; Chloramphenicol; Drug Resistance, Bacterial; Erythromycin; Kanamycin; Lipase; Microbial Sensitivity Tests; Molecular Typing; Penicillins; Phylogeny; Pseudomonas; RNA, Ribosomal, 16S; Soil Microbiology; Tetracycline
PubMed: 24405763
DOI: 10.1186/1476-511X-13-9 -
Indian Journal of Ophthalmology Aug 2018A 60-year-old male presented with pain and decreased vision 3 weeks following uneventful intracapsular cataract extraction with anterior vitrectomy for subluxated...
A 60-year-old male presented with pain and decreased vision 3 weeks following uneventful intracapsular cataract extraction with anterior vitrectomy for subluxated cataract. A diagnosis of acute endophthalmitis was made based on clinical and ultrasound features. Patient improved only after undergoing pars plana vitrectomies twice and repeated intravitreal antibiotic-steroid injections. Vitreous aspirate revealed Gram-negative bacillus identified as Pseudomonas luteola on culture. Patient returned with a retinal detachment at first follow-up which was treated with vitrectomy, endolaser, and silicone oil tamponade. To the best of our knowledge, this is the first case of P. luteola causing acute onset, virulent endophthalmitis reported in literature.
Topics: Acute Disease; Cataract Extraction; Endophthalmitis; Eye Infections, Bacterial; Humans; India; Male; Middle Aged; Pseudomonas; Pseudomonas Infections; Surgical Wound Infection; Ultrasonography; Vitrectomy
PubMed: 30038182
DOI: 10.4103/ijo.IJO_242_18 -
BMC Veterinary Research Apr 2015In captive breed turtles and tortoises conjunctival disease is common. Our aim was to investigate the bacterial and fungal flora present in the eyes of healthy and...
BACKGROUND
In captive breed turtles and tortoises conjunctival disease is common. Our aim was to investigate the bacterial and fungal flora present in the eyes of healthy and pathological chelonians and to compare findings in turtles with those in tortoises.
RESULTS
Samples were taken from the conjunctival sacs of 34, diseased and healthy, chelonians (18 tortoises and 16 turtles) and submitted to bacterial and fungal investigation. All samples showed bacterial growth. Thirteen animals (38%), harboured a single bacterial species as sole isolate and twenty-one animals (62%) harboured more than one species. Detection of multiple bacterial infection was clearly greater in tortoises compared to turtles. Most frequently isolated bacterial species were Bacillus spp. (13 isolates), Staphylococcus xylosus (10 isolates), Sphingomonas paucimobilis (6 isolates), Staphylococcus sciuri and Aeromonas hydrophila/caviae (each 5 isolates), Ochrobactrum anthropi (3 isolates), Citrobacter freundii, Enterobacter cloacae and Pseudomonas luteola (each 2 isolates). Only one isolate of Kocuria varians/rosea, Staphylococcus aureus, Staphylococcus auricularis, Staphylococcus haemolyticus, Staphylococcus lentus, Morganella morganii, Pasteurella multocida, Pasteurella pneumotropica/haemolytica, Proteus spp., Pseudomonas putida, Salmonella enterica ssp. arizonae, Stenotrophomonas maltophilia and Vibrio parahaemolyticus was evidenced. The presence in 8 animals of Mycoplasma spp. and in 1 animal with severe conjunctivitis of Chlamydia spp. was detected by PCR. Candida spp. was also isolated from two healthy animals.
CONCLUSIONS
A clear predominance of Gram positive isolates in tortoises and Gram negative isolates in turtles was found. However, we cannot ascribe the observed difference to the diversity of animal species, as other factors, including especially different characteristics of the living environments, may play a role. Almost all bacterial species isolated may have clinical significance, mostly as opportunistic pathogens, both for humans and animals. That chelonians are often carrier of bacteria with zoonotic potential is a well-known fact, in particular with regard to Salmonella spp. Therefore, it is not surprising the detection of a strain of Salmonella enterica ssp. arizonae in the eye of one of the animals tested. Worthy of note is the finding of Chlamydia spp. in a severe case of conjunctivitis, though we cannot epidemiologically assess a cause-effect relationship between presence of chlamydia and disease.
Topics: Animals; Bacteria; Bacterial Infections; Case-Control Studies; Conjunctivitis; Turtles
PubMed: 25889261
DOI: 10.1186/s12917-015-0405-x