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Neurology India 2023
Topics: Humans; Bacillus cereus; Meningoencephalitis
PubMed: 37635561
DOI: 10.4103/0028-3886.383861 -
International Journal of Molecular... Jun 2023Plant growth-promoting bacteria (PGPB) appear to be a sensible competitor to conventional fertilization, including mineral fertilizers and chemical plant protection... (Review)
Review
Plant growth-promoting bacteria (PGPB) appear to be a sensible competitor to conventional fertilization, including mineral fertilizers and chemical plant protection products. Undoubtedly, one of the most interesting bacteria exhibiting plant-stimulating traits is, more widely known as a pathogen, . To date, several environmentally safe strains of have been isolated and described, including WSE01, MEN8, YL6, SA1, ALT1, ERBP, GGBSTD1, AK1, AR156, C1L, and T4S. These strains have been studied under growth chamber, greenhouse, and field conditions and have shown many significant traits, including indole-3-acetic acid (IAA) and aminocyclopropane-1-carboxylic acid (ACC) deaminase production or phosphate solubilization, which allows direct plant growth promotion. It includes an increase in biometrics traits, chemical element content (e.g., N, P, and K), and biologically active substances content or activity, e.g., antioxidant enzymes and total soluble sugar. Hence, has supported the growth of plant species such as soybean, maize, rice, and wheat. Importantly, some strains can also promote plant growth under abiotic stresses, including drought, salinity, and heavy metal pollution. In addition, strains produced extracellular enzymes and antibiotic lipopeptides or triggered induced systemic resistance, which allows indirect stimulation of plant growth. As far as biocontrol is concerned, these PGPB can suppress the development of agriculturally important phytopathogens, including bacterial phytopathogens (e.g., , , and ), fungal phytopathogens (e.g., , , and ), and other phytopathogenic organisms (e.g., (Nematoda) and (Protozoa)). In conclusion, it should be noted that there are still few studies on the effectiveness of under field conditions, particularly, there is a lack of comprehensive analyses comparing the PGP effects of and mineral fertilizers, which should be reduced in favor of decreasing the use of mineral fertilizers. It is also worth mentioning that there are still very few studies on the impact of on the indigenous microbiota and its persistence after application to soil. Further studies would help to understand the interactions between and indigenous microbiota, subsequently contributing to increasing its effectiveness in promoting plant growth.
Topics: Bacillus cereus; Fertilizers; Plant Development; Phosphates
PubMed: 37298706
DOI: 10.3390/ijms24119759 -
Plants (Basel, Switzerland) Nov 2023Berliner () and sensu stricto Frankland and Frankland are closely related species of aerobic, spore-forming bacteria included in the sensu lato group. This group is... (Review)
Review
Berliner () and sensu stricto Frankland and Frankland are closely related species of aerobic, spore-forming bacteria included in the sensu lato group. This group is one of the most studied, but it remains also the most mysterious species of bacteria. Despite more than a century of research on the features of these ubiquitous bacteria, there are a lot of questionable issues related to their taxonomy, resistance to external influences, endophytic existence, their place in multidimensional relationships in the ecosystem, and many others. The review summarizes current data on the mutualistic relationships of and bacteria with plants, the structure of the phytomicrobiomes including and , and the abilities of plant-associated and endophytic strains to improve plant resistance to various environmental factors and its productivity. Key findings on the possibility of the use of Cry gene promoter for transcription of the target dsRNA and simultaneous release of pore-forming proteins and provocation of RNA-interference in pest organisms allow us to consider this group of microorganisms as unique tools of genetic engineering and biological control. This will open the prospects for the development and direct change of plant microbiomes, and possibly serve as the basis for the regulation of the entire agroecosystem.
PubMed: 38068672
DOI: 10.3390/plants12234037 -
Revista Argentina de Microbiologia 2023Biofilm formation by Bacillus cereus strains is now recognized as a systematic contamination mechanism in foods; the aim of this study was to evaluate the production of...
Biofilm formation by Bacillus cereus strains is now recognized as a systematic contamination mechanism in foods; the aim of this study was to evaluate the production of submerged and interface biofilms in strains of B. cereus group in different materials, the effect of dextrose, motility, the presence of genes related to biofilms and the enterotoxigenic profile of the strains. We determine biofilm production by safranin assay, motility on semi-solid medium, toxin gene profiling and genes related to biofilm production by PCR in B. cereus group isolated from food. In this study, we observe strains used a higher production of biofilms in PVC; in the BHI broth, no submerged biofilms were found compared to phenol red broth and phenol red broth supplemented with dextrose; no strains with the ces gene were found, the enterotoxin profile was the most common the profile that includes genes for the three enterotoxins. We observed a different distribution of tasA and sipW with the origin of isolation of the strain, being more frequent in the strains isolated from eggshell. The production and type of biofilms are differential according to the type of material and culture medium used.
Topics: Bacillus; Bacillus cereus; Phenolsulfonphthalein; Enterotoxins; Food Microbiology; Biofilms; Glucose
PubMed: 37019800
DOI: 10.1016/j.ram.2023.01.007 -
Infection Prevention in Practice Jun 2023Outbreaks of bloodstream infections (BSIs) are a concern in Japanese medical settings.
BACKGROUND
Outbreaks of bloodstream infections (BSIs) are a concern in Japanese medical settings.
AIM
This study determined baseline values for detection in clinical samples that are useful as reference values for hospitals when assessing the need for intervention.
METHOD
A retrospective analysis of detection in the Japan Nosocomial Infections Surveillance data from 2008 to 2014 was performed; it included 950 individual hospitals across the country.
FINDINGS
spp. were detected in 0.54% of the clinical specimens submitted for bacteriological testing. Specimens positive for spp. were mainly blood (24.6%), stool (26.5%), and respiratory specimens (23.3%). Identification of spp. at the species level (i.e., or ) was reported in 55.3%, 14.7%, and 15.4% of cases, of which 88.9%, 48.3%, and 33.1% were in blood, stool, and respiratory specimens, respectively. Of the 4105 hospital-years, 75.7% had blood specimens with spp., with a median of 0.85 blood specimens/100 beds annually (interquartile range, 0.17-2.10). The detection showed significant summer seasonality, regardless of specimen type or geographic distribution. The detection did not show seasonality, and its detection remained constant throughout the year. The seasonality of spp. reflects the high proportion of .
CONCLUSIONS
The increased detection rate of spp. during summer should be interpreted as a risk factor for BSIs. A post-summer decrease in spp. should not be interpreted as an effect of interventions.
PubMed: 36910424
DOI: 10.1016/j.infpip.2023.100272 -
Medicine Jul 2023This study aimed to investigate the clinical characteristics, management and prognosis of Bacillus cereus sepsis in premature neonates. The clinical information of 8...
This study aimed to investigate the clinical characteristics, management and prognosis of Bacillus cereus sepsis in premature neonates. The clinical information of 8 premature neonates with B cereus sepsis who were treated in Shanghai Children Hospital from January 2015 to December 2019 was retrospectively collected from the medical records and analyzed. The neurodevelopment related conditions were collected at follow up visits at corrected age of 6 months and 12 months. Five patients developed meningitis, and cerebral magnetic resonance image showed abnormal in 5 patients. After treatment with meropenem and vancomycin, 1 patient died, and 7 patients survived and were smoothly discharged. At follow up visits, 1 patient was diagnosed with hydrocephalus and showed severely delayed neurodevelopment, 2 patients had mild delayed neurodevelopment, and the neurodevelopment was basically normal in remaining 4 patients. B cereus infection can cause severe complications of central nervous system, and affect neurodevelopmental outcome. Antibiotic treatment with meropenem and vancomycin is proven to be effective. Refreshing the central catheters is helpful for the prevention of B cereus sepsis and cerebral magnetic resonance image may be employed for the prognosis assessment.
Topics: Infant, Newborn; Child; Humans; Infant; Vancomycin; Bacillus cereus; Meropenem; Retrospective Studies; China; Sepsis; Prognosis; Infant, Newborn, Diseases
PubMed: 37443518
DOI: 10.1097/MD.0000000000034261 -
Heliyon Jun 2023The prevalence of emetic strains in food products is rare; however, infection with these may be fatal to the vulnerable population. Bacterial control of the emetic...
The prevalence of emetic strains in food products is rare; however, infection with these may be fatal to the vulnerable population. Bacterial control of the emetic strains is still unclear. This study aimed to assess the influence of high temperature on the disinfection of emetic and enterotoxigenic . Emetic strains survived up to 50 °C; the lag time and maximum growth rate were higher at 42 °C than those at 30 °C. Compared to enterotoxigenic , all emetic food strains showed higher minimum inhibitory concentrations and minimum bactericidal concentrations for sodium hypochlorite and citric acid. The disinfectant susceptibility of the emetic OS-05 strain incubated at a higher temperature did not increase and was maintained at the highest MBC value. In all emetic strains, enterotoxin gene expression was upregulated at 42 °C and 45 °C. Increased gene expression was also found in emetic strains GP-15 and OS-05, with upregulation of 128- and 820-fold at 42 °C. Thus, emetic grown at high temperatures may resist common disinfectants of the food industry. The findings may help control in food or the food processing industry.
PubMed: 37484226
DOI: 10.1016/j.heliyon.2023.e16863 -
Molecules (Basel, Switzerland) Oct 2023() is a common foodborne pathogen causing food poisoning incidents. This study aimed to evaluate the antibacterial activity and underlying mechanism of rhamnolipids...
() is a common foodborne pathogen causing food poisoning incidents. This study aimed to evaluate the antibacterial activity and underlying mechanism of rhamnolipids (RLs) against . The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of RLs for were determined to be 16.0 mg/L and 32.0 mg/L, respectively. Scanning electron microscopy and fluorescence microscope images, as well as data of membrane potential, relative electric conductivity, and leakage of intracellular components revealed that RLs disrupted the integrity of the cell membrane. Furthermore, the reactive oxygen species content, catalase (CAT) and superoxide dismutase (SOD) activity indicated that RLs activated the oxidative stress response of in response to RLs. Fresh wet noodles (FWN) were used as a food model, and RLs showed a significant killing effect on with a sustained inhibitory effect at the concentrations ranging from 128.0 to 1024.0 mg/kg. Additionally, RLs promoted the conversion of free water to bound water in FWN, which improved the storage of FWN and made the taste more resilient and chewy. These results suggest that RLs could be a potential alternative to antimicrobial agents and preservatives for applications in food processing.
Topics: Bacillus cereus; Anti-Bacterial Agents; Anti-Infective Agents; Water
PubMed: 37836789
DOI: 10.3390/molecules28196946 -
Biochimie Jan 2024Overexpression of recombinant Bacillus cereus TSPO (BcTSPO) in E. coli bacteria leads to its recovery with a bound hemin both in bacterial membrane (MB) and inclusion...
Overexpression of recombinant Bacillus cereus TSPO (BcTSPO) in E. coli bacteria leads to its recovery with a bound hemin both in bacterial membrane (MB) and inclusion bodies (IB). Unlike mouse TSPO, BcTSPO purified in SDS detergent from IB is well structured and can bind various ligands such as high-affinity PK 11195, protoporphyrin IX (PPIX) and δ-aminolevulinic acid (ALA). For each of the three ligands, H-N HSQC titration NMR experiments suggest that different amino acids of BcTSPO binding cavity are involved in the interaction. PPIX, an intermediate of heme biosynthesis, binds to the cavity of BcTSPO and its fluorescence can be significantly reduced in the presence of light and oxygen. The light irradiation leads to two products that have been isolated and characterized as photoporphyrins. They result from the addition of singlet oxygen to the two vinyl groups hence leading to the formation of hydroxyaldehydes. The involvement of water molecules, recently observed along with the binding of heme in Rhodobacter sphaeroides (RsTSPO) is highly probable. Altogether, these results raise the question of the role of TSPO in heme biosynthesis regulation as a possible scavenger of reactive intermediates.
PubMed: 38280504
DOI: 10.1016/j.biochi.2024.01.011