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Medicine Jun 2018Controversy exists regarding whether the kinesiology tape application direction affects muscle strength. (Randomized Controlled Trial)
Randomized Controlled Trial
BACKGROUND
Controversy exists regarding whether the kinesiology tape application direction affects muscle strength.
METHODS
Eighteen healthy volunteers (12 men, 6 women) participated. Kinesiology tape was randomly applied to the quadriceps muscles either from origin to insertion or from insertion to origin. A Biodex isokinetic dynamometer was used to measure the peak torque of the quadriceps pre-and post-taping.
RESULTS
There was a significant difference in muscle strength after taping, regardless of the kinesiology tape application direction. There were no significant differences in the peak torque of the quadriceps between the 2 kinesiology tape application directions.
CONCLUSIONS
The application of kinesiology tape application to the rectus femoris, vastus medialis, and vastus lateralis of the quadriceps increased the muscle torque, regardless of the tape application direction. Therefore, to enhance quadriceps strength, we recommend the application of kinesiology tape to 3 of the muscles of the quadriceps (specifically, the rectus femoris, vastus medialis and vastus lateralis), irrespective of the tape application direction.
Topics: Adult; Athletic Tape; Cross-Over Studies; Female; Healthy Volunteers; Humans; Kinesiology, Applied; Male; Muscle Strength; Muscle Strength Dynamometer; Quadriceps Muscle; Torque
PubMed: 29901599
DOI: 10.1097/MD.0000000000011038 -
Dermatitis : Contact, Atopic,... Oct 2021Tape-strips are a minimally invasive approach to characterize skin biomarkers in atopic dermatitis (AD). However, they have not yet been used for tracking gene...
BACKGROUND
Tape-strips are a minimally invasive approach to characterize skin biomarkers in atopic dermatitis (AD). However, they have not yet been used for tracking gene expression changes with systemic treatment.
OBJECTIVE
The aim of the study was to evaluate gene expression changes and therapeutic response biomarkers in AD patients before and after dupilumab (interleukin 4Rα antibody) treatment using tape-strips to obtain epidermal tissue for analysis.
METHODS
Lesional and nonlesional tape-stripped skin was sampled from 18 AD patients before and after dupilumab treatment and from 17 healthy subjects and analyzed by RNA-seq.
RESULTS
At baseline, we detected 6745 and 4859 differentially expressed genes between lesional and nonlesional skin versus normal, respectively, whereas 841 and 977 genes were differentially expressed after treatment, respectively (fold change >1.5 and false discovery rate <0.05). Tape-strips captured significant modulation with dupilumab in key AD immune (eg, C-C motif chemokine ligand 13 [CCL13], CCL17, CCL18) and barrier (eg, periplakin, FA2H) biomarkers. Changes in biomarkers (CCL20, interleukin 34, FABP7) were also significantly correlated with clinical disease improvements (Eczema Area and Severity Index; R > 0.5 or R < -0.4, P < 0.05).
CONCLUSIONS
This real-life study represents the first comprehensive RNA-seq molecular profiling of tape-strips from moderate to severe AD patients after dupilumab therapy. Analysis of tape strip specimens detected significant gene expression changes in key AD biomarkers with dupilumab treatment, suggesting that this approach may be useful to monitor therapeutic responses in inflammatory skin diseases.
Topics: Adult; Antibodies, Monoclonal, Humanized; Case-Control Studies; Dermatitis, Atopic; Female; Gene Expression Profiling; Humans; Male; Middle Aged; Sequence Analysis, RNA; Skin
PubMed: 34405829
DOI: 10.1097/DER.0000000000000764 -
Cell Dec 2023Patient-derived organoids (PDOs) can model personalized therapy responses; however, current screening technologies cannot reveal drug response mechanisms or how tumor...
Patient-derived organoids (PDOs) can model personalized therapy responses; however, current screening technologies cannot reveal drug response mechanisms or how tumor microenvironment cells alter therapeutic performance. To address this, we developed a highly multiplexed mass cytometry platform to measure post-translational modification (PTM) signaling, DNA damage, cell-cycle activity, and apoptosis in >2,500 colorectal cancer (CRC) PDOs and cancer-associated fibroblasts (CAFs) in response to clinical therapies at single-cell resolution. To compare patient- and microenvironment-specific drug responses in thousands of single-cell datasets, we developed "Trellis"-a highly scalable, tree-based treatment effect analysis method. Trellis single-cell screening revealed that on-target cell-cycle blockage and DNA-damage drug effects are common, even in chemorefractory PDOs. However, drug-induced apoptosis is rarer, patient-specific, and aligns with cancer cell PTM signaling. We find that CAFs can regulate PDO plasticity-shifting proliferative colonic stem cells (proCSCs) to slow-cycling revival colonic stem cells (revCSCs) to protect cancer cells from chemotherapy.
Topics: Humans; Apoptosis; Cancer-Associated Fibroblasts; Organoids; Signal Transduction; Single-Cell Analysis; Drug Evaluation, Preclinical; Algorithms; Stem Cells
PubMed: 38065081
DOI: 10.1016/j.cell.2023.11.005 -
Proceedings of the National Academy of... Nov 2012Medical tape that provides secure fixation of life-sustaining and -monitoring devices with quick, easy, damage-free removal represents a longstanding unmet medical need...
Medical tape that provides secure fixation of life-sustaining and -monitoring devices with quick, easy, damage-free removal represents a longstanding unmet medical need in neonatal care. During removal of current medical tapes, crack propagation occurs at the adhesive-skin interface, which is also the interface responsible for device fixation. By designing quick-release medical tape to undergo crack propagation between the backing and adhesive layers, we decouple removal and device fixation, enabling dual functionality. We created an ordered adhesive/antiadhesive composite intermediary layer between the medical tape backing and adhesive for which we achieve tunable peel removal force, while maintaining high shear adhesion to secure medical devices. We elucidate the relationship between the spatial ordering of adhesive and antiadhesive regions to create a fully tunable system that achieves strong device fixation and quick, easy, damage-free device removal. We also described ways of neutralizing the residual adhesive on the skin and have observed that thick continuous films of adhesive are easier to remove than the thin islands associated with residual adhesive left by current medical tapes.
Topics: Adhesives; Humans; Materials Testing; Skin
PubMed: 23112196
DOI: 10.1073/pnas.1216071109 -
Cell Dec 2023Cancer cells are regulated by oncogenic mutations and microenvironmental signals, yet these processes are often studied separately. To functionally map how...
Cancer cells are regulated by oncogenic mutations and microenvironmental signals, yet these processes are often studied separately. To functionally map how cell-intrinsic and cell-extrinsic cues co-regulate cell fate, we performed a systematic single-cell analysis of 1,107 colonic organoid cultures regulated by (1) colorectal cancer (CRC) oncogenic mutations, (2) microenvironmental fibroblasts and macrophages, (3) stromal ligands, and (4) signaling inhibitors. Multiplexed single-cell analysis revealed a stepwise epithelial differentiation phenoscape dictated by combinations of oncogenes and stromal ligands, spanning from fibroblast-induced Clusterin (CLU) revival colonic stem cells (revCSCs) to oncogene-driven LRIG1 hyper-proliferative CSCs (proCSCs). The transition from revCSCs to proCSCs is regulated by decreasing WNT3A and TGF-β-driven YAP signaling and increasing KRAS or stromal EGF/Epiregulin-activated MAPK/PI3K flux. We find that APC loss and KRAS collaboratively limit access to revCSCs and disrupt stromal-epithelial communication-trapping epithelia in the proCSC fate. These results reveal that oncogenic mutations dominate homeostatic differentiation by obstructing cell-extrinsic regulation of cell-fate plasticity.
Topics: Cell Differentiation; Oncogenes; Proto-Oncogene Proteins p21(ras); Signal Transduction; Stem Cells; Humans; Animals; Mice; Cell Lineage
PubMed: 38065080
DOI: 10.1016/j.cell.2023.11.004 -
Analytical Chemistry Oct 2019A noninvasive electrochemical melanoma detection approach based on using adhesive tapes for collecting and fixing cells from a suspicious skin area and transferring the...
A noninvasive electrochemical melanoma detection approach based on using adhesive tapes for collecting and fixing cells from a suspicious skin area and transferring the cells into a scanning electrochemical microscope (SECM) is presented. The adhesive layer collects the cells reproducibly and keeps them well adhered on the tape during experiments in an electrolyte solution. A melanoma biomarker, here the intracellular enzyme tyrosinase (TYR), was imaged on the tape-collected cells without further cell lysing using antibodies that were labeled with horseradish peroxidase (HRP). The HRP labels catalyzed the oxidation of a dissolved redox-active species, which was detected at a soft microelectrode, gently brushed in contact mode over the tape. The melanoma biomarker was first detected on tape-stripped samples with murine melanoma cells of different concentrations. Thereafter, increasing levels of TYR were recorded in cells that were collected from the skin of melanoma mouse models representing three different stages of tumor growth. Additionally, SECM results of tape-stripped different human melanoma cell lines were confirmed by previous studies based on traditionally fixed and permeabilized cells.
Topics: Adhesives; Animals; Antibodies, Monoclonal; Biomarkers, Tumor; Catalysis; Horseradish Peroxidase; Humans; Melanoma, Experimental; Mice; Microscopy, Electrochemical, Scanning; Monophenol Monooxygenase; Oxidation-Reduction; Skin Neoplasms; Tumor Cells, Cultured
PubMed: 31529964
DOI: 10.1021/acs.analchem.9b02819 -
Scientific Reports Mar 2019There is a great interest in low-cost, versatile microfluidic platforms of which the fabrication processes are rapid, straightforward, and translatable to industrial...
There is a great interest in low-cost, versatile microfluidic platforms of which the fabrication processes are rapid, straightforward, and translatable to industrial mass productions. In addition, it is beneficial for microfluidic devices to be reconfigurable in the field, so that multiple functions can be realized by a minimum number of devices. Here, we present a versatile acrylic-tape platform which allows highly accessible rapid prototyping of microfluidic devices, as well as device reconfiguration to realize different functions. The clean-room-free fabrication and sealing process only requires a laser cutter, acrylic, and tapes and can be done by an untrained person in the field. We experimentally characterized the relationship between the capillary flow speed and the channel height, the latter of which can be well controlled by the fabrication process. Reconfiguration of microfluidic functions was demonstrated on a single acrylic-tape device, thanks to the reversible sealing enabled by functional tapes. Different pumping mechanisms, including on-chip pumps for better portability and syringe pumps for precise fluid control, have been employed for the demonstration of two-phase flow and droplet generation, respectively. The low-cost and versatile acrylic-tape microfluidic devices are promising tools for applications in a wide range of fields, especially for point-of-care biomedical and clinical applications.
PubMed: 30886239
DOI: 10.1038/s41598-019-41208-y -
European Journal of Physical and... Jun 2018Extracorporeal Shock Wave Therapy (ESWT) is effective in the treatment of calcific tendinopathy of the rotator cuff, eliciting an analgesic/anti-inflammatory action and... (Randomized Controlled Trial)
Randomized Controlled Trial
BACKGROUND
Extracorporeal Shock Wave Therapy (ESWT) is effective in the treatment of calcific tendinopathy of the rotator cuff, eliciting an analgesic/anti-inflammatory action and promoting tissue regeneration. Kinesio taping (KT), another recently-introduced rehabilitative tool, exerts an analgesic and biomechanical action on joints and muscles. ESWT and KT may have a synergic effect when used in combination, but the effectiveness of the association has not been established.
AIM
The aim of this study was to test if the association of KT with ESWT is superior to ESWT alone in the treatment of rotator cuff calcific tendinopathy.
DESIGN
Randomized controlled trial.
SETTING
Rehabilitation Institute outpatients.
POPULATION
Forty-two patients with rotator cuff calcific tendinopathy were randomly assigned to the experimental group (ESWT+KT, N.=21) or control (ESWT, N.=21).
METHODS
In the experimental group, patients underwent three sessions (once a week for 3 weeks) of ESWT with KT applied at the end of each session. Controls underwent three sessions of ESWT only. All patients were assessed before treatment (T0) and at 1 (T1), 4 (T2) and 12 weeks (T3) after the end of treatment with the following outcome measures: a visual analogue scale (VAS), the Disabilities of the Arm, Shoulder and Hand (DASH) questionnaire, Subjective Shoulder Rating Questionnaire (SSRQ), and Oxford Shoulder Score (OSS).
RESULTS
Both groups showed significant improvement in all outcome measures, but the time course differed between the two groups. At T1 vs. T0, the improvement was significantly better in ESWT+KT than ESWT on VAS (P=0.007), DASH (P<0.0001) and SSRQ (P=0.0001). Successive improvements at T2 vs. T1 and T3 vs. T2 did not differ significantly between the groups. At the end of follow-up, ESWT+KT still showed significantly greater improvement than ESWT on VAS (P=0.02) and SSRQ (P=0.038).
CONCLUSIONS
KT associated with ESWT seems to improve the recovery in rotator cuff calcific tendinopathy with a faster therapeutic response compared to ESWT only.
CLINICAL REHABILITATION IMPACT
Our results suggest the effectiveness of using KT as adjuvant therapy to ESWT in rotator cuff calcific tendinopathy, through enhancing the short-term analgesic action and the medium- to long-term biological-regenerative effects.
Topics: Adult; Aged; Ambulatory Care; Analysis of Variance; Athletic Tape; Calcinosis; Combined Modality Therapy; Extracorporeal Shockwave Therapy; Female; Humans; Italy; Magnetic Resonance Imaging; Male; Middle Aged; Pain Measurement; Prognosis; Range of Motion, Articular; Rehabilitation Centers; Risk Assessment; Rotator Cuff; Shoulder Joint; Shoulder Pain; Tendinopathy; Treatment Outcome
PubMed: 29185674
DOI: 10.23736/S1973-9087.17.04749-9 -
Journal of Biomedical Nanotechnology Dec 2015The advanced technologies available for micro/nanofabrication have opened new avenues for interdisciplinary approaches to solve the unmet medical needs of regenerative... (Review)
Review
The advanced technologies available for micro/nanofabrication have opened new avenues for interdisciplinary approaches to solve the unmet medical needs of regenerative medicine and biomedical devices. This review highlights the recent developments in micro/nanostructured adhesives and films for biomedical applications, including waterproof seals for wounds or surgery sites, drug delivery, sensing human body signals, and optical imaging of human tissues. We describe in detail the fabrication processes required to prepare the adhesives and films, such as tape-based adhesives, nanofilms, and flexible and stretchable film-based electronic devices. We also discuss their biomedical functions, performance in vitro and in vivo, and the future research needed to improve the current systems.
Topics: Adhesives; Animals; Biocompatible Materials; Humans; Mechanical Phenomena; Microtechnology; Nanostructures; Nanotechnology
PubMed: 26510305
DOI: 10.1166/jbn.2015.2097 -
Methods in Cell Biology 2023In this chapter, we review Automated Tape Collecting Ultramicrotomy (ATUM), which, among other array tomography methods, substantially simplified large-scale volume... (Review)
Review
In this chapter, we review Automated Tape Collecting Ultramicrotomy (ATUM), which, among other array tomography methods, substantially simplified large-scale volume electron microscopy (vEM) projects. vEM reveals biological structures at nanometer resolution in three dimensions and resolves ambiguities of two-dimensional representations. However, as the structures of interest-like disease hallmarks emerging from neuropathology-are often rare but the field of view is small, this can easily turn a vEM project into a needle in a haystack problem. One solution for this is correlated light and electron microscopy (CLEM), providing tissue context, dynamic and molecular features before switching to targeted vEM to hone in on the object's ultrastructure. This requires precise coordinate transfer between the two imaging modalities (e.g., by micro computed tomography), especially for block face vEM which relies on physical destruction of sections. With array tomography methods, serial ultrathin sections are collected into a tissue library, thus allowing storage of precious samples like human biopsies and enabling repetitive imaging at different resolution levels for an SEM-based search strategy. For this, ATUM has been developed to reliably collect serial ultrathin sections via a conveyor belt onto a plastic tape that is later mounted onto silicon wafers for serial scanning EM (SEM). The ATUM-SEM procedure is highly modular and can be divided into sample preparation, serial ultramicrotomy onto tape, mounting, serial image acquisition-after which the acquired image stacks can be used for analysis. Here, we describe the steps of this workflow and how ATUM-SEM enables targeting and high resolution imaging of specific structures. ATUM-SEM is widely applicable. To illustrate this, we exemplify the approach by reconstructions of focal pathology in an Alzheimer mouse model and CLEM of a specific cortical synapse.
Topics: Mice; Animals; Humans; Microscopy, Electron, Scanning; Volume Electron Microscopy; X-Ray Microtomography; Microtomy; Neurons; Imaging, Three-Dimensional
PubMed: 37451765
DOI: 10.1016/bs.mcb.2023.01.012