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Antonie Van Leeuwenhoek Apr 2014The significance of Brevibacillus has been documented scientifically in the published literature and commercially in heterologous recombinant protein catalogs.... (Review)
Review
The significance of Brevibacillus has been documented scientifically in the published literature and commercially in heterologous recombinant protein catalogs. Brevibacillus is one of the most widespread genera of Gram-positive bacteria, recorded from the diverse environmental habitats. The high growth rate, better transformation efficiency by electroporation, availability of shuttle vectors, production of negligible amount of extracellular protease, and the constitutive expression of heterologous proteins make some strains of this genus excellent laboratory models. Regarding biotechnological applications, this genus continues to be a source of various enzymes of great biotechnological interest due to their ability to biodegrade low density polyethylene, ability to act as a candidate bio-control agent, and more recently acknowledged as a tool for the overexpression. This article reviews the properties of Brevibacillus spp. as better biological tools with varied applications.
Topics: Biodegradation, Environmental; Biotechnology; Brevibacillus; Cultured Milk Products; Phylogeny; Recombinant Proteins
PubMed: 24563152
DOI: 10.1007/s10482-013-0099-7 -
World Journal of Microbiology &... Mar 2018Species that are currently listed under the genus Brevibacillus (formerly, Bacillus brevis cluster) have been a rich source of antimicrobial peptides for many decades.... (Review)
Review
Species that are currently listed under the genus Brevibacillus (formerly, Bacillus brevis cluster) have been a rich source of antimicrobial peptides for many decades. The first known peptide antibiotic, gramicidin, is presumed to be produced by a Brevibacillus sp. Members of the genus are widely spread in nature. They can be found in a variety of environments including intestinal tracts of animals, seawater, and soil. Some Brevibacillus strains have been used commercially as probiotics. Bioactive peptides produced by Brevibacillus spp. include antibacterial, antifungal and anti-invertebrate agents. Brevibacillus antimicrobial peptides are synthesized through ribosomal or nonribosomal pathway; these two groups can be further categorized based on specific structural features such as cyclization and presence of lipid chain. Some of the antimicrobial compounds produced by this genus share structural similarities that were overlooked previously. For example, the structural similarity between BT peptide, brevibacillin, and bogorol was revealed only recently. Here we review and classify Brevibacillus antimicrobial peptides and summarize their bioactivities and potential applications.
Topics: Animals; Anti-Bacterial Agents; Antifungal Agents; Antimicrobial Cationic Peptides; Bacteriocins; Brevibacillus; Edeine; Gramicidin; Guanidines; Lipopeptides; Peptides; Peptides, Cyclic; Probiotics; Ribosomes; Seawater; Soil Microbiology; Tyrocidine
PubMed: 29594558
DOI: 10.1007/s11274-018-2437-4 -
Microorganisms Mar 2024() is widely distributed in nature and demonstrates significant potential for applications in biological control, environmental protection, agricultural production, and... (Review)
Review
() is widely distributed in nature and demonstrates significant potential for applications in biological control, environmental protection, agricultural production, and clinical medicine. This review provides a comprehensive overview of the applications of in crop cultivation and animal feeding, as well as an examination of the antimicrobial peptides produced by and their antibacterial mechanisms. enhances crop cultivation by secreting hydrolases to improve nutrient absorption capabilities, synthesizing hormones to promote crop growth, and producing proteins to inhibit the reproduction of harmful organisms. has been used to improve animal production by regulating the structure of the intestinal microbiota and inhibiting the growth of pathogenic bacteria through the secretion of various antimicrobial peptides. The bactericidal activity of Brevilaterins secreted by is attributed to their ability to bind to lipopolysaccharide/lipid II molecules on the cell membrane, thereby altering permeability. Brevilaterins also inhibit bacterial reproduction by affecting relevant gene pathways in the cell membranes of pathogenic bacteria. These pathways include ATP synthesis, peptidoglycan biosynthesis, membrane transport, and cellular metabolism. In conclusion, exhibits substantial potential as a probiotic activity in crop and animal production. However, applications of in animal production could be improved, necessitating further research to elucidate the underlying probiotic mechanisms.
PubMed: 38543615
DOI: 10.3390/microorganisms12030564 -
International Journal of Systematic and... Dec 2023An aerobic, Gram-stain-positive, rod-shaped, endospore-forming bacterial strain, designated BB3-R1, was isolated from cow faeces sampled in Daejeon, Republic of Korea....
An aerobic, Gram-stain-positive, rod-shaped, endospore-forming bacterial strain, designated BB3-R1, was isolated from cow faeces sampled in Daejeon, Republic of Korea. Growth was observed at 25-45 °C (optimum, 35-40 °C) and pH 7.0-9.0 (optimum, pH 8.0), with up to 3 % (w/v) NaCl (optimum, 0 % NaCl). blast analysis of 16S rRNA gene sequences revealed the highest sequence similarity of strain BB3-R1 to NRRL NRS-818 (98.8 %) followed by JCM 15085 (97.5 %). According to 16S rRNA gene and whole-genome based phylogenetic trees, strain BB3-R1 clustered with FJAT-54423 and NRRL NRS-818. OrthoANI and dDDH values of strain BB3-R1 with the closely related strains were lower than 77.5 and 26.8 %, respectively. The major menaquinones and polar lipids of the strain were MK-7 and phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, respectively. The major fatty acids (>10 %) were C iso, C iso, C anteiso and C 7 alcohol. The cell-wall peptidoglycan contained cross-linked -diaminopimelic acid (type A1 gamma). The phenotypic, chemotaxonomic and genotypic data obtained in this study showed that the strain represents a novel species of the genus , for which the name sp. nov. (type strain BB3-R1=KACC 22663=NBRC 115962) is proposed.
Topics: Cattle; Animals; Brevibacillus; Fatty Acids; Phospholipids; Sodium Chloride; Sequence Analysis, DNA; Phylogeny; RNA, Ribosomal, 16S; Bacterial Typing Techniques; DNA, Bacterial; Base Composition
PubMed: 38097527
DOI: 10.1099/ijsem.0.006204 -
Current Microbiology Dec 2022A new aerobic bacterial strain, designated strain YIM B02290, was isolated from the soil of Machangqing, Dali city, Yunnan Province, China. Cells were...
A new aerobic bacterial strain, designated strain YIM B02290, was isolated from the soil of Machangqing, Dali city, Yunnan Province, China. Cells were Gram-stain-positive, sporogenous, rod-shaped, and motile with peritrichous flagella. Strain YIM B02290 showed the highest 16S rRNA gene sequence similarity with Brevibacillus laterosporus (97.6%) and Brevibacillus halotolerans (97.6%). The ANI and dDDH values between strain YIM B02290 and the two reference strains Brevibacillus laterosporus LAM00312 and Brevibacillus halotolerans DSM 25 are 72.6% and 72.2%, 20.2% and 19.5% based on the draft genome sequence, respectively. The major cellular fatty acids contain anteiso-C and iso-C. The diagnostic diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid. The predominant menaquinone was identified as menaquinone-7. The main polar lipids of strain YIM B02290 were diphosphatidylglycerol, phosphatidylglycerol, phospholipid, phosphatidyl monomethylethanolamine. The genomic DNA G + C content was 40.6 mol%. All results showed that strain YIM B02290 represents a novel species of the genus Brevibacillus, for which the name Brevibacillus daliensis sp. nov. is proposed. The type strain is YIM B02290 (= CCTCC AB 2021094 = CGMCC 1.18802 = KCTC 43376).
Topics: Soil; RNA, Ribosomal, 16S; China
PubMed: 36480068
DOI: 10.1007/s00284-022-03127-6 -
Cureus Sep 2020() is an aerobic gram-positive bacillus that is rarely associated with human infection. A review of multiple online databases revealed no other cases of bacteremia in...
() is an aerobic gram-positive bacillus that is rarely associated with human infection. A review of multiple online databases revealed no other cases of bacteremia in an adult involving this organism. Historically, this "canoe-shaped" microbe has been characterized as a pathogen in invertebrates, and information regarding human infection is scarce. We present a clinical vignette of what we believe to be the first reported case of bacteremia in an adult human subject.
PubMed: 33083182
DOI: 10.7759/cureus.10481 -
International Journal of Systematic and... Nov 2021Two aerobic, Gram-stain-positive, rod-shaped, endospore-forming, thermophilic bacterial strains, designated FJAT-54423 and FJAT-54424, were isolated from...
Two aerobic, Gram-stain-positive, rod-shaped, endospore-forming, thermophilic bacterial strains, designated FJAT-54423 and FJAT-54424, were isolated from hyperthermophilic compost sampled in Shanxi Province, PR China. Growth was observed at 30-60 °C (optimum, 50 °C) and pH 6.0-9.0 (optimum, pH 7.0), with up to 2.0 % (w/v) NaCl (optimum, 0 % NaCl). The 16S rRNA gene sequence similarity between FJAT-54423 and FJAT-54424 was 99.9%, and the maximum similarity to a valid taxon was observed with (98.3%). Further, in phylogenetic and phylogenomic trees, strains FJAT-54423 and FJAT-54424 branched with members of the genus . The menaquinone was MK-7, and the major fatty acids were iso-C and anteiso-C. The main polar lipids included phosphatidylmethylethanolamine, phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The cell-wall peptidoglycan was found to contain -diaminopimelic acid. The DNA G+C content of strains FJAT-54423 and FJAT-54424 were 54.3 and 54.4 mol%, respectively. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423 and its most closely related reference strain DSM 6347 were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%). Based on the observed physiological properties, chemotaxonomic characteristics and ANI and dDDH values, FJAT-54423 and FJAT-54424 belong to a novel species of the genus , for which the name sp. nov. is proposed. The type strain is FJAT-54423 (=GDMCC 1.2054=KCTC 43273).
Topics: Bacterial Typing Techniques; Base Composition; Brevibacillus; China; Composting; DNA, Bacterial; Diaminopimelic Acid; Fatty Acids; Nucleic Acid Hybridization; Peptidoglycan; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Soil Microbiology; Vitamin K 2
PubMed: 34762578
DOI: 10.1099/ijsem.0.005098 -
Available strategies for improved expression of recombinant proteins in expression system: a review.Critical Reviews in Biotechnology Nov 2020offers great potential as a recombinant protein expression host because of its exceptional abilities to synthesize and excrete proteins and its low extracellular... (Review)
Review
offers great potential as a recombinant protein expression host because of its exceptional abilities to synthesize and excrete proteins and its low extracellular protease activity. Despite these strengths, effective recombinant expression strategies are still the key to achieving high-level expression of recombinant proteins in due to individual differences among strains and target proteins. Many strategies have been developed to improve recombinant protein expression in . This review begins by introducing the processes used to establish and apply the expression system, and then critically discusses the strategies available for improving recombinant protein expression in , including optimization of the host and the expression vector, co-expression of a fusion partner or foldase, and optimization of the fermentation process. Finally, the prospects for further improvement of recombinant protein expression based on are also discussed. This review is intended to provide a strategic reference for scientists wanting to improve the expression of a specific recombinant protein in or other expression systems.
Topics: Brevibacillus; Cloning, Molecular; Fermentation; Genetic Engineering; Genetic Vectors; Plasmids; Promoter Regions, Genetic; Recombinant Fusion Proteins
PubMed: 32781847
DOI: 10.1080/07388551.2020.1805404 -
Insects Sep 2013Brevibacillus laterosporus, a bacterium characterized by the production of a unique canoe-shaped lamellar body attached to one side of the spore, is a natural inhabitant... (Review)
Review
Brevibacillus laterosporus, a bacterium characterized by the production of a unique canoe-shaped lamellar body attached to one side of the spore, is a natural inhabitant of water, soil and insects. Its biopesticidal potential has been reported against insects in different orders including Coleoptera, Lepidoptera, Diptera and against nematodes and mollusks. In addition to its pathogenicity against invertebrates, different B. laterosporus strains show a broad-spectrum antimicrobial activity including activity against phytopathogenic bacteria and fungi. A wide variety of molecules, including proteins and antibiotics, have been associated with the observed pathogenicity and mode of action. Before being considered as a biological control agent against plant pathogens, the antifungal and antibacterial properties of certain B. laterosporus strains have found medical interest, associated with the production of antibiotics with therapeutic effects. The recent whole genome sequencing of this species revealed its potential to produce polyketides, nonribosomal peptides, and toxins. Another field of growing interest is the use of this bacterium for bioremediation of contaminated sites by exploiting its biodegradation properties. The aim of the present review is to gather and discuss all recent findings on this emerging entomopathogen, giving a wider picture of its complex and broad-spectrum biocontrol activity.
PubMed: 26462431
DOI: 10.3390/insects4030476 -
Current Microbiology May 2022A Gram-positive-staining, strictly aerobic, motile, ellipsoidal endospore-forming bacterial strain, designated CHY01, was isolated from the Chishui river in a section of...
A Gram-positive-staining, strictly aerobic, motile, ellipsoidal endospore-forming bacterial strain, designated CHY01, was isolated from the Chishui river in a section of Maotai Town, Guizhou Province, Southwest China. Strain CHY01 was found to grow optimally at pH 8.0 and 28 °C. The 16S rRNA gene sequence analysis indicated that strain CHY01 belonged to the genus Brevibacillus and clustered with the type strain of Brevibacillus panacihumi, with which it exhibited 16S rRNA gene sequence similarity values of 97.8%. The predominant respiratory quinone was MK-7, and the major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The major fatty acids were C, iso-C, anteiso-C, C, Ciso-H and/or C 3-OH, and C and/or C. Genome sequencing revealed a genome size of 6.1 Mbp and a G + C content of 50.6%. The results of physiological and biochemical tests allowed strain CHY01 to be distinguished genotypically and phenotypically from Brevibacillus species with validly published names. Pairwise determined whole-genome average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values suggested that strain CHY01 represents a new species, for which we propose the name Brevibacillus dissolubilis sp. nov. with the type strain CHY01 (= CGMCC 1.15916 = KCTC 33863).
Topics: Bacterial Typing Techniques; Brevibacillus; DNA, Bacterial; Fatty Acids; Fresh Water; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 35579716
DOI: 10.1007/s00284-022-02891-9