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Archives of Microbiology Jul 2021Two bacterial strains, designated REN4 and REN4-1, were isolated from daqu sample collected from baijiu factory located in Shanxi, China. The two strains shared highly...
Two bacterial strains, designated REN4 and REN4-1, were isolated from daqu sample collected from baijiu factory located in Shanxi, China. The two strains shared highly similar 16S rRNA gene sequences (99.67% identities) and formed a monophyletic clade within the Brevibacterium 16S rRNA gene tree, showing 97.56-97.85% 16S rRNA gene sequence identities with type strains Brevibacterium permense VKM Ac-2280, Brevibacterium sediminis FXJ8.269, Brevibacterium oceani BBH7 and Brevibacterium epidermidis NCIMB 702286. They contained MK-8(H) as the most predominant menaquinone, antesio-C, antesio-C, Iso-C and Iso-C as the major cellular fatty acids, DPG (diphosphatidylglycerol), PG (phosphatidylglycerol), PGL (phosphatidylglycerollipids), and PL (phospholipids) as the main polar lipids. The genomic DNA G + C content of strains REN4 and REN4-1 were 64.35, 65.82 mol%. Moreover, the low DNA-DNA relatedness values, physiological and biochemical characteristics, and taxonomic analysis allowed the differentiation of strains REN4 and REN4-1 from the other recognized species of the genus Brevibacterium. Therefore, strain REN4 represents a novel species of the genus Brevibacterium, for which the name Brevibacterium renqingii sp. nov. is proposed, with the type strain REN4 (= JCM 33953 T = KCTC 49366).
Topics: Bacterial Typing Techniques; Base Composition; Brevibacterium; DNA, Bacterial; Diaminopimelic Acid; Fatty Acids; Fermentation; Fermented Foods; Nucleic Acid Hybridization; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 33649904
DOI: 10.1007/s00203-021-02212-x -
Mayo Clinic Proceedings Apr 2021
Topics: Aged; Brevibacterium; Female; Humans; Male; Middle Aged; Sepsis
PubMed: 33814079
DOI: 10.1016/j.mayocp.2021.01.022 -
International Journal of Systematic and... Oct 2020A Gram stain-positive, non-spore-forming, non-motile and rod-shaped actinomycete, strain 5221, was isolated from the sediment of a river collected at Ronggui in the...
A Gram stain-positive, non-spore-forming, non-motile and rod-shaped actinomycete, strain 5221, was isolated from the sediment of a river collected at Ronggui in the Pearl River Delta, PR China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain formed a distinct lineage within the genus and had the highest sequence similarity to Tp12 (96.7 %), followed by 2C6-41 (96.5 %), SST-8 (96.0 %) and 20 (95.9 %). The results of chemotaxonomic analyses, including detecting anteiso-C, anteiso-C, and C as the major cellular fatty acids, diphosphatidylglycerol, phosphatidylglycerol and three phosphoglycolipids as the polar lipids, MK-8(H) as the major menaquinone, and a DNA G+C content of 72.4 mol%, supported that strain 5221 is a member of the genus . Furthermore, low sequence similarities of 16S rRNA gene sequences, differences in fatty acid compositions and differential physiological characteristics such as enzyme activity and carbon sources utilization ability distinguished the isolate from its close relatives. Therefore, strain 5221 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain 5221 (=GDMCC 1.1766=KACC 21700).
Topics: Bacterial Typing Techniques; Base Composition; Brevibacterium; China; DNA, Bacterial; Fatty Acids; Geologic Sediments; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Rivers; Sequence Analysis, DNA; Vitamin K 2
PubMed: 32816658
DOI: 10.1099/ijsem.0.004379 -
Biotechnology Letters Apr 2005The genus Brevibacterium has long been difficult for taxonomists to classify due to its close morphological similarity to other genera. Since it was proposed in 1953,... (Review)
Review
The genus Brevibacterium has long been difficult for taxonomists to classify due to its close morphological similarity to other genera. Since it was proposed in 1953, the genus has often been redefined. The genus is best known for its important role in the ripening of certain cheeses (B. linens) and for its supposed over-production of L: -amino acids. Other interesting industrial applications, including the production of ectoine, have recently been proposed. The general characteristics, the occurrence and the recent taxonomy of Brevibacterium are reviewed here. Furthermore, known and potential industrial applications for Brevibacterium species are briefly discussed.
Topics: Amino Acids; Anti-Bacterial Agents; Brevibacterium; Carotenoids; Industrial Microbiology
PubMed: 15973485
DOI: 10.1007/s10529-005-2878-3 -
International Journal of Systematic and... Sep 2018A Gram-positive, strictly aerobic, non-motile, milky-white to creamy coloured and rod-shaped bacterium, designated BS05, was isolated from compost. Phylogenetic analysis...
A Gram-positive, strictly aerobic, non-motile, milky-white to creamy coloured and rod-shaped bacterium, designated BS05, was isolated from compost. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to Brevibacterium avium NCFB 3055 (96.3 %), Brevibacterium oceani BBH7 (96.2 %) and Brevibacterium epidermidis NBRC 14811 (96.1 %). The DNA G+C content was 62.3 mol%. The predominant quinone was MK-8(H2). The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and iso-C15 : 0. The cell-wall peptidoglycan of strain BS05 contained meso-diaminopimelic acid. The major polar lipid was phosphatidylglycerol. Moreover, the low sequence similarity of the 16S rRNA gene sequencing, physiological, biochemical and chemotaxonomic analyses allowed the phenotypic and genotypic differentiation of strain BS05 from the recognized species of the genus Brevibacterium. Therefore, strain BS05 represents a novel species of the genus Brevibacterium, for which the name Brevibacteriumhankyongi sp. nov. is proposed, with the type strain BS05 (=KACC 18875=LMG 29562).
Topics: Bacterial Typing Techniques; Base Composition; Brevibacterium; Composting; DNA, Bacterial; Diaminopimelic Acid; Fatty Acids; Nucleic Acid Hybridization; Peptidoglycan; Phosphatidylglycerols; Phylogeny; RNA, Ribosomal, 16S; Republic of Korea; Sequence Analysis, DNA; Soil Microbiology
PubMed: 30063198
DOI: 10.1099/ijsem.0.002886 -
Scientific Reports Apr 2019The genus Brevibacterium harbors many members important for cheese ripening. We performed real-time quantitative PCR (qPCR) to determine the abundance of Brevibacterium...
The genus Brevibacterium harbors many members important for cheese ripening. We performed real-time quantitative PCR (qPCR) to determine the abundance of Brevibacterium on rinds of Vorarlberger Bergkäse, an Austrian artisanal washed-rind hard cheese, over 160 days of ripening. Our results show that Brevibacterium are abundant on Vorarlberger Bergkäse rinds throughout the ripening time. To elucidate the impact of Brevibacterium on cheese production, we analysed the genomes of three cheese rind isolates, L261, S111, and S22. L261 belongs to Brevibacterium aurantiacum, whereas S111 and S22 represent novel species within the genus Brevibacterium based on 16S rRNA gene similarity and average nucleotide identity. Our comparative genomic analysis showed that important cheese ripening enzymes are conserved among the genus Brevibacterium. Strain S22 harbors a 22 kb circular plasmid which encodes putative iron and hydroxymethylpyrimidine/thiamine transporters. Histamine formation in fermented foods can cause histamine intoxication. We revealed the presence of a putative metabolic pathway for histamine degradation. Growth experiments showed that the three Brevibacterium strains can utilize histamine as the sole carbon source. The capability to utilize histamine, possibly encoded by the putative histamine degradation pathway, highlights the importance of Brevibacterium as key cheese ripening cultures beyond their contribution to cheese flavor production.
Topics: Adaptation, Physiological; Austria; Brevibacterium; Cheese; Fermentation; Genome, Bacterial; Histamine; Metabolic Networks and Pathways; Plasmids
PubMed: 30992535
DOI: 10.1038/s41598-019-42525-y -
Journal of Microbiology (Seoul, Korea) Oct 2018Gram-positive, aerobic, non-motile, pale-yellow, and rodshaped bacterium, designated as Gsoil 188, was isolated from the soil of a ginseng field in Pocheon, South Korea....
Gram-positive, aerobic, non-motile, pale-yellow, and rodshaped bacterium, designated as Gsoil 188, was isolated from the soil of a ginseng field in Pocheon, South Korea. A phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Brevibacterium and was most closely related to B. epidermidis NBRC 14811 (98.4%), B. sediminis FXJ8.269 (98.2%), B. avium NCFB 3055 (98.1%), and B. oceani BBH7 (98.1%), while it shared less than 98.1% identity with the other species of this genus. The DNA G + C content was 68.1 mol%. The predominant quinone was MK-8(H). The major fatty acids were anteiso-C and anteiso-C. The cell wall peptidoglycan of strain Gsoil 188 contained meso-diaminopimelic acid. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, and an unidentified aminolipid. The physiological and biochemical characteristics, low DNA-DNA relatedness values, and taxonomic analysis allowed the differentiation of strain Gsoil 188 from the other recognized species of the genus Brevibacterium. Therefore, strain Gsoil 188 represents a novel species of the genus Brevibacterium, for which the name Brevibacterium anseongense sp. nov. is proposed, with the type strain Gsoil 188 (= KACC 19439 = LMG 30331).
Topics: Bacterial Typing Techniques; Base Composition; Brevibacterium; DNA, Bacterial; Diaminopimelic Acid; Fatty Acids; Panax; Phosphatidylglycerols; Phylogeny; RNA, Ribosomal, 16S; Republic of Korea; Sequence Analysis, DNA; Soil Microbiology
PubMed: 30136258
DOI: 10.1007/s12275-018-8181-5 -
Revista Chilena de Infectologia :... Feb 2021
Topics: Actinomycetales Infections; Brevibacterium; Humans
PubMed: 33844798
DOI: 10.4067/S0716-10182021000100099 -
Journal of Microbiology (Seoul, Korea) Oct 2021During a study of the marine actinobacterial biodiversity, a large number of Brevibacterium strains were isolated. Of these, five that have relatively low 16S rRNA gene...
Brevibacterium limosum sp. nov., Brevibacterium pigmenatum sp. nov., and Brevibacterium atlanticum sp. nov., three novel dye decolorizing actinobacteria isolated from ocean sediments.
During a study of the marine actinobacterial biodiversity, a large number of Brevibacterium strains were isolated. Of these, five that have relatively low 16S rRNA gene similarity (98.5-99.3%) with validly published Brevibacterium species, were chosen to determine taxonomic positions. On the basis of 16S rRNA gene sequence analysis and BOX-PCR fingerprinting, strains o2, YB235, and WO024 were selected as representative strains. Genomic analyses, including average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH), clearly differentiated the three strains from each other and from their closest relatives, with values ranging from 82.8% to 91.5% for ANI and from 26.7% to 46.5% for dDDH that below the threshold for species delineation. Strains YB235, WO024, and o2 all exhibited strong and efficient decolorization activity in congo red (CR) dyes, moderate decolorization activity in toluidine blue (TB) dyes and poor decolorization in reactive blue (RB) dyes. Genes coding for peroxidases and laccases were identified and accounted for these strains' ability to effectively oxidize a variety of dyes with different chemical structures. Mining of the whole genome for secondary metabolite biosynthesis gene clusters revealed the presence of gene clusters encoding for bacteriocin, ectoine, NRPS, siderophore, T3PKS, terpene, and thiopeptide. Based on the phylogenetic, genotypic and phenotypic data, strains o2, YB235 and WO024 clearly represent three novel taxa within the genus Brevibacterium, for which the names Brevibacterium limosum sp. nov. (type strain o2 = JCM 33844 = MCCC 1A09961), Brevibacterium pigmenatum sp. nov. (type strain YB235 = JCM 33843 = MCCC 1A09842) and Brevibacterium atlanticum sp. nov. (type strain WO024 = JCM 33846 = MCCC 1A16743) are proposed.
Topics: Bacterial Typing Techniques; Biodegradation, Environmental; Brevibacterium; China; Coloring Agents; DNA, Bacterial; Fatty Acids; Geologic Sediments; Oceans and Seas; Phylogeny; RNA, Ribosomal, 16S; Seawater
PubMed: 34491521
DOI: 10.1007/s12275-021-1235-0 -
International Journal of Systematic and... Nov 2020A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium, designated O1, was isolated from a deep-sea sediment of the Western Pacific Ocean. Strain...
A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium, designated O1, was isolated from a deep-sea sediment of the Western Pacific Ocean. Strain O1 showed optimal growth at 30 °C, between pH 6.0 and 8.0, and in the presence of 1-5 % (w/v) NaCl. The predominant menaquinone was MK-8 (H), and anteiso-C and anteiso-C were the major fatty acids. The diagnostic diamino acid in the cell-wall peptidoglycan was -diaminopimelic acid. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and one unknown glycolipid. The DNA G+C content of strain O1 was 64.9 mol% and the genome size was 4.17 Mb. Based on a similarity search and phylogenetic analysis of the 16S rRNA gene sequence, strain O1 belonged to the genus . The values of average nucleotide identity and DNA-DNA hybridization between strain O1 and its close relatives were well below the thresholds used for the delineation of a new species. On the basis of the morphological and chemotaxonomic characteristics, as well as the genotypic data, it is proposed that strain O1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is O1 (=JCM 33845=MCCC 1A16744).
Topics: Bacterial Typing Techniques; Base Composition; Brevibacterium; DNA, Bacterial; Diaminopimelic Acid; Fatty Acids; Genome Size; Geologic Sediments; Nucleic Acid Hybridization; Pacific Ocean; Peptidoglycan; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Vitamin K 2
PubMed: 32965208
DOI: 10.1099/ijsem.0.004483