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Chemosphere Jan 2021Natural occurring metal-tolerant microbial population have replaced conventional expensive metal remediation approach since the last few years. The present study focuses...
Natural occurring metal-tolerant microbial population have replaced conventional expensive metal remediation approach since the last few years. The present study focuses on investigating the potential of a copper-tolerant plant growth promoting rhizobacterial strain Brevundimonas diminuta MYS6 for Cu bioremediation, plant growth promotion and Cu uptake in Helianthus annuus L. Box-Behnken Design of response surface methodology optimized the influencing parameters such as pH, temperature and Cu concentration. At optimized conditions of pH (5), temperature (32.5 °C) and Cu concentration (250 mg/L), the rhizobacteria followed a sigmoid growth curve pattern with maximum Cu removal of 94.8% in the stationary phase of growth. Cu exposed Brevundimonas diminuta MYS6 produced increased EPS (18.6%), indicating their role in internal defence against Cu stress. The FTIR analysis suggested the role of carboxylic acids, alcohols and aliphatic amine groups in Cu bioremoval. Furthermore, the results of pot experiments conducted with Helianthus annuus L. var. CO4 and Brevundimonas diminuta MYS6 showed enhanced plant growth and Cu uptake. The rhizobacteria increased root and shoot length, fresh and dry plant biomass and leaf chlorophyll by 1.5, 1.7, 9.9, 15.8 and 2.1 fold. The plant biomass mediate enhanced Cu uptake in roots and shoots was found to be 2.98 and 4.1 folds higher when compared to non-inoculated treatment. Henceforth the results of the study evidence the rhizobacterial strain Brevundimonas diminuta MYS6 as an efficient bio-inoculant for copper remediation.
Topics: Biodegradation, Environmental; Caulobacteraceae; Copper; Helianthus; Plant Roots; Soil Pollutants
PubMed: 33297160
DOI: 10.1016/j.chemosphere.2020.128195 -
Eye & Contact Lens Jan 2012To report the first documented case of keratitis secondary to the gram-negative bacillus Brevundimonas diminuta.
PURPOSE
To report the first documented case of keratitis secondary to the gram-negative bacillus Brevundimonas diminuta.
METHODS
A 66-year-old woman with an ocular history of bilateral soft contact lens wear presented with a large, 6×9-mm, culture-positive Pseudomonas aeruginosa-induced corneal ulcer. After weeks of therapy, she developed secondary keratitis. Repeat cultures grew a rare, gram-negative bacillus, B. diminuta.
RESULTS
Targeted therapy followed by adjustments based on repeat bacterial cultures with sensitivities resulted in slow resolution of the ulcer. After months of therapy, the cornea recovered leaving only a minimal corneal scar. Visual acuity improved from a low of Hand Motions to a final 20/40.
CONCLUSIONS
This is the first reported case of ocular infection with B. diminuta. This case demonstrates the need to be aware of potential rare secondary infections in corneas compromised by an initial infection and to adjust pharmacotherapy based on sensitivities and clinical signs.
Topics: Aged; Anti-Bacterial Agents; Caulobacteraceae; Female; Gram-Negative Bacterial Infections; Humans; Keratitis; Visual Acuity
PubMed: 21617535
DOI: 10.1097/ICL.0b013e31821c04f7 -
Virulence Jan 2018Non-fermenting Gram-negative bacteria are problematic in clinical locations, being one of the most prevalent causes of nosocomial infections. Many of these... (Review)
Review
Non-fermenting Gram-negative bacteria are problematic in clinical locations, being one of the most prevalent causes of nosocomial infections. Many of these non-fermenting Gram-negative bacteria are opportunistic pathogens that affect patients that are suffering with underlying medical conditions and diseases. Brevundimonas spp., in particular Brevundimonas diminuta and Brevundimonas vesicularis, are a genus of non-fermenting Gram-negative bacteria considered of minor clinical importance. Forty-nine separate instances of infection relating to Brevundimonas spp were found in the scientific literature along with two pseudo-infections. The majority of these instances were infection with Brevundimonas vesicularis (thirty-five cases - 71%). The major condition associated with Brevundimonas spp infection was bacteraemia with seventeen individual cases/outbreaks (35%). This review identified forty-nine examples of Brevundimonas spp. infections have been discussed in the literature. These findings indicate that infection review programs should consider investigation of possible Brevundimonas spp outbreaks if these bacteria are clinically isolated in more than one patient.
Topics: Bacteremia; Caulobacteraceae; Communicable Diseases, Emerging; Disease Outbreaks; Global Health; Gram-Negative Bacterial Infections; Humans; Opportunistic Infections
PubMed: 29484917
DOI: 10.1080/21505594.2017.1419116 -
The Science of the Total Environment Mar 2022The toxic metal(loid)s TMs resistant bacterium Brevundimonas diminuta was isolated for the first time from mines polluted soil in Fengxian, China, and assessed for its...
Brevundimonas diminuta isolated from mines polluted soil immobilized cadmium (Cd) and zinc (Zn) through calcium carbonate precipitation: Microscopic and spectroscopic investigations.
The toxic metal(loid)s TMs resistant bacterium Brevundimonas diminuta was isolated for the first time from mines polluted soil in Fengxian, China, and assessed for its potential for Cd and Zn precipitation in Cd and Zn co-contaminated aqueous solution at various Cd and Zn levels (20, 40, 80, 160, and 200 mg L), pH values (5, 6, 7, 8, and 9), and temperatures (20, 25, 30, and 35 °C). B. diminuta showed a high resistance to both Cd and Zn and was able to precipitate up to 99.2 and 99.7% of dissolved Cd and Zn respectively, at a pH of 7 and temperature of 30 °C. B. diminuta reduced the dissolved concentrations of Cd and Zn below the threshold levels in water. The 3D-EEM analysis revealed the presence of extracellular polymeric substances (EPS) such as tryptophan indicating bacterial growth under Cd/Zn stress. FTIR showed polysaccharides, CO, CaCO, PO, and proteins, which may enhance bacterial growth and metal precipitation. SEM-EDS confirmed the leaf-like and granular shape of the biological precipitation and reduction in the percent weight of TMs, which promoted the adhesion/adsorption of Cd, Zn, and Ca. Moreover, XRD analysis confirmed the precipitation of Cd, Zn, and Ca in the form of CdCO/Cd(PO), ZnCO/ZnHPO/Zn(OH)PO/Zn(PO), and CaCO/Ca(PO)OH, respectively. These findings indicate that Brevundimonas diminuta can be used for the bioremediation of TMs-contaminated aquatic environments.
Topics: Cadmium; Calcium Carbonate; Caulobacteraceae; Soil; Soil Pollutants; Zinc
PubMed: 34963589
DOI: 10.1016/j.scitotenv.2021.152668 -
BMJ Case Reports May 2021, a non-fermenting gram-negative bacterium, is emerging as an important multidrug resistant opportunistic pathogen. It has been described in cases of bacteremia,...
, a non-fermenting gram-negative bacterium, is emerging as an important multidrug resistant opportunistic pathogen. It has been described in cases of bacteremia, pleuritis, keratitis and peritoneal dialysis-associated peritonitis. We describe, for the first time, a case of pyogenic liver abscess caused by coinfection of and , and briefly review pyogenic liver abscesses and the literature regarding .
Topics: Caulobacteraceae; Coinfection; Humans; Liver Abscess, Pyogenic; Streptococcus anginosus
PubMed: 33975829
DOI: 10.1136/bcr-2020-236235 -
Microorganisms Apr 2023is broadly distributed in terrestrial and aquatic environments and has various biological activities. In this study, we found that exhibited nematicidal activity...
is broadly distributed in terrestrial and aquatic environments and has various biological activities. In this study, we found that exhibited nematicidal activity against the plant root-knot nematode, . A total of 42 volatile organic compounds (VOCs) from were identified using gas chromatography-mass spectrometry (GC-MS). The nematicidal activity of the 10 main VOCs was tested against . Butyl butanoate (4 µL) caused the mortality of 80.13% of after 4 h. The nematicidal activity of an additional 38 butyl-butyrate-like volatile esters was also investigated. Of these, seven had strong nematicidal activity against , five of which showed egg-hatching inhibitory activity. This study is the first to report that butyl butanoate, ethyl 2-methylbutanoate, ethyl 4-methylpentanoate, ethyl pent-4-enoate, and methyl undecanoate have nematicidal activity against . The results indicated that could serve as a candidate microorganism for the biocontrol of plant root-knot nematodes, showing that volatile esters have great potential as nematicides.
PubMed: 37110389
DOI: 10.3390/microorganisms11040966 -
Cureus Jul 2023Lung abscesses caused by () are a rare occurrence, particularly in immunocompetent adults. We present the case of a 47-year-old male with a history of COPD, bipolar...
Lung abscesses caused by () are a rare occurrence, particularly in immunocompetent adults. We present the case of a 47-year-old male with a history of COPD, bipolar disorder, and seizure disorder, who presented with a productive cough, worsening shortness of breath, yellow sputum, weight loss, and fatigue over a period of three weeks. Clinical examination revealed decreased breath sounds in the left upper lung zones. Laboratory investigations showed an elevated white cell count, while blood cultures identified . Imaging with computed tomography (CT) confirmed the presence of a 4.2x2.0 cm cavitary lesion consistent with a lung abscess. The patient was successfully treated with a combination of Ampicillin/Sulbactam and Azithromycin, followed by a course of oral Augmentin. Given the size of the abscess and favorable response to antibiotic therapy, invasive procedures were deemed unnecessary. This case underscores the importance of considering unusual pathogens in the etiology of lung abscesses, even in immunocompetent individuals, and highlights the successful management with appropriate antibiotic therapy.
PubMed: 37621835
DOI: 10.7759/cureus.42371 -
Microbiology Resource Announcements Mar 2019Here, we describe the complete genome sequence of the ATCC(B) 19146 strain, which contains 3,375 protein-coding genes and 61 RNAs within its 3,551,819-bp-long genome....
Here, we describe the complete genome sequence of the ATCC(B) 19146 strain, which contains 3,375 protein-coding genes and 61 RNAs within its 3,551,819-bp-long genome. The genome consists of a circular chromosome.
PubMed: 30923241
DOI: 10.1128/MRA.00083-19 -
PDA Journal of Pharmaceutical Science... 2015Brevundimonas diminuta is the organism most commonly used for challenge testing of sterilizing-grade filter membranes. ASTM F838-05 and PDA Technical Report 26 rely on... (Comparative Study)
Comparative Study
UNLABELLED
Brevundimonas diminuta is the organism most commonly used for challenge testing of sterilizing-grade filter membranes. ASTM F838-05 and PDA Technical Report 26 rely on B. diminuta ATCC #19146 for standard challenge tests used to designate sterilizing-grade filter performance. Despite the importance and widespread use of B. diminuta in filter testing and validation, information about this microorganism in its native hydrated state is limited. In this work, we measure, for the first time, the mechanical property of modulus for B. diminuta cultured in saline lactose broth (as described in ASTM F838-05) via wet atomic force microscopy. For comparison, we also imaged B. diminuta by the traditional method of electron microscopy after capture on a filter and chemical fixation. The modulus of hydrated B. diminuta cells was ∼193 mPa. To put this result into context, a simple model for pore penetration that correlates the role of the Young's modulus of hydrated cells to the penetration of sterilizing-grade filters is proposed. The model confirms the industry experience that pore size is an essential parameter in preventing the penetration of B. diminuta into sterilizing-grade filters.
LAY ABSTRACT
The small microorganism Brevundimonas diminuta is used to characterize the performance of sterilizing-grade filter membranes used in the manufacturing of sterile drug products. Little is known about the size, shape, or elasticity of living bacterial cells, as it is easier to characterize bacteria after chemical fixation in a dry state. In this work, we use atomic force microscopy to determine the size, shape, and deformability of this important microorganism while it is alive and fully hydrated. Additionally, we compare the physical and mechanical properties of B. diminuta measured in wet and dry states. This information can be used to advance our understanding of how filter membranes remove these organisms from fluid streams.
Topics: Caulobacteraceae; Elastic Modulus; Filtration; Microscopy, Atomic Force; Microscopy, Electron; Sterilization
PubMed: 26048743
DOI: 10.5731/pdajpst.2015.01045 -
Indian Journal of Pathology &... 2017Brevundimonas has rarely been isolated from clinical specimens. We here report a case of Brevundimonas diminuta infection in an 18-year-old male patient with nephrotic...
Brevundimonas has rarely been isolated from clinical specimens. We here report a case of Brevundimonas diminuta infection in an 18-year-old male patient with nephrotic syndrome. B. diminuta was identified by the VITEK 2 Compact system, following isolation from his blood sample. To the best of our knowledge, B. diminuta has not been reported in patients with nephrotic syndrome.
Topics: Adolescent; Bacteriological Techniques; Blood; Caulobacteraceae; Gram-Negative Bacterial Infections; Humans; Male; Nephrotic Syndrome
PubMed: 28631656
DOI: 10.4103/IJPM.IJPM_679_15