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International Journal of Systematic and... Jan 2013A Gram-negative, rod-shaped, weakly motile, non-spore-forming bacterium (D9(T)) was isolated from the gut of Cylindroiulus fulviceps (Diplopoda) on 1/3-strength nutrient...
A Gram-negative, rod-shaped, weakly motile, non-spore-forming bacterium (D9(T)) was isolated from the gut of Cylindroiulus fulviceps (Diplopoda) on 1/3-strength nutrient agar plates. On the basis of 16S rRNA gene sequence similarity, strain D9(T) was shown to be phylogenetically closely related to the type strain of Budvicia aquatica, the sole species of the genus Budvicia, family Enterobacteriaceae. The similarity of the 16S rRNA gene sequences of strain D9(T) and B. aquatica DSM 5075(T) was 98.4 %. Other strains that showed high pairwise similarities with the isolate belonged to the genus Yersinia: Y. frederiksenii ATCC 33641(T) (96.8 % 16S rRNA gene sequence similarity), Y. massiliensis CCUG 53443(T) (96.8 %), Y. pestis NCTC 5923(T) (96.8 %), Y. pseudotuberculosis ATCC 29833(T) (96.8 %), Y. similis CCUG 52882(T) (96.7 %) and Y. ruckeri ATCC 29473(T) (96.5 %). The similarities of sequences of the housekeeping genes rpoB, hsp60 and gyrB between strain D9(T) and B. aquatica DSM 5075(T) and other members of the Enterobacteriaceae were less than 94 %. Phylogenetic trees based on all four gene sequences unequivocally grouped the isolate with the type strain of B. aquatica and separately from the genus Yersinia. Cells contained the quinones Q-8, Q-7 and MK-8. The major polar lipids were phosphatidylglycerol and phosphatidylethanolamine. The G+C content of the DNA (48.3 mol%) and the whole-cell fatty acid composition of strain D9(T) (C(14 : 0), C(16 : 1)ω7c, C(16 : 0), cyclo-C(17 : 0) and C(18 : 1)ω7c as major components) were typical for members of the Enterobacteriaceae. DNA-DNA hybridization of strain D9(T) with B. aquatica DSM 5075(T) resulted in a relatedness of 30.4 %, indicating that the isolate did not belong to B. aquatica. Physiological tests allowed the phenotypic differentiation of strain D9(T) from B. aquatica DSM 5075(T) as well as from members of the genus Yersinia. From these results, it is concluded that strain D9(T) represents a novel species, for which the name Budvicia diplopodorum sp. nov. is proposed (type strain D9(T) =DSM 21983(T) = CCM 7845(T)). The description of the genus Budvicia is emended.
Topics: Animals; Arthropods; Bacterial Typing Techniques; Base Composition; DNA, Bacterial; Enterobacteriaceae; Fatty Acids; Molecular Sequence Data; Nucleic Acid Hybridization; Phylogeny; Quinones; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 22407790
DOI: 10.1099/ijs.0.036749-0 -
Journal of Medical Microbiology Aug 2007Budvicia aquatica has been found in surface water not associated with human faeces or sewage. The first known case is described of infection in an 85-year-old woman...
Budvicia aquatica has been found in surface water not associated with human faeces or sewage. The first known case is described of infection in an 85-year-old woman exposed to the aftermath of Hurricane Katrina, who tested positive for B. aquatica from both blood and urine samples.
Topics: Aged, 80 and over; Anti-Infective Agents; Ciprofloxacin; Disasters; Enterobacteriaceae; Enterobacteriaceae Infections; Female; Humans; Immunocompromised Host; Sepsis
PubMed: 17644725
DOI: 10.1099/jmm.0.47139-0 -
Applied and Environmental Microbiology Dec 2005Electron microscopic analysis of contractile phage tail-like bacteriocins of three Pragia fontium strains and one Budvicia aquatica strain was performed. Fonticin and...
Electron microscopic analysis of contractile phage tail-like bacteriocins of three Pragia fontium strains and one Budvicia aquatica strain was performed. Fonticin and aquaticin are remarkably heat sensitive but trypsin resistant. Simultaneous production of contractile and flexible phage tail-like bacteriocins in the P. fontium 64613 strain is shown for the first time.
Topics: Bacteriocins; Bacteriophages; Enterobacteriaceae; Microscopy, Electron; Molecular Weight; Sensitivity and Specificity
PubMed: 16332902
DOI: 10.1128/AEM.71.12.8970-8973.2005 -
Journal of Hygiene, Epidemiology,... 1984
Topics: Enterobacteriaceae; Phenotype; Water Microbiology
PubMed: 6470483
DOI: No ID Found -
Journal of Hygiene, Epidemiology,... 1992SDS PAGE protein patterns of 37 H2S-positive strains of species belonging to the family Enterobacteriaceae including the genera Budvicia (11 strains) and Leminorella (L.... (Comparative Study)
Comparative Study
SDS PAGE protein patterns of 37 H2S-positive strains of species belonging to the family Enterobacteriaceae including the genera Budvicia (11 strains) and Leminorella (L. grimontii--3 strains, L. richardii--4 strains) were compared with 10 strains of species Pragia fontium. All strains under study form well separated clusters with overall similarity C = .49. Clusters are separated in the range of C = .68-.83. They display high homogeneity, only one strain of Edwardsiella tarda clusters with budviciae. Strains of Pragia form two distinct clusters separated from other genera. Electrophoretograms of two strains which do not group as expected are analyzed and results discussed. Results support evidence that strains designated Pragia fontium deserve independent treatment as a new species.
Topics: Bacterial Proteins; Electrophoresis, Polyacrylamide Gel; Enterobacteriaceae; Microbiological Techniques; Phenotype
PubMed: 1512457
DOI: No ID Found -
Mikrobiolohichnyi Zhurnal (Kiev,... 2012The fatty acid composition of lipopolysaccharides (LPS) lipids A of Budvicia aquatica strains (n = 6)--representatives of Enterobacteriaceae new species are studied for...
The fatty acid composition of lipopolysaccharides (LPS) lipids A of Budvicia aquatica strains (n = 6)--representatives of Enterobacteriaceae new species are studied for the first time. It was established that fatty acids with the length of carbon chains from C12 to C18 are presented. All of B. aquatica strains tested have been found to contain 3-hydroxytetradecanoic acid (23.1-43.8%, depending on the strain), which was predominat and characteristic of representatives of Enterobacteriaceae family. LPS of the tested strains displayed toxicity and pyrogeneity.
Topics: Animals; Anti-Inflammatory Agents, Non-Steroidal; Body Temperature; Enterobacteriaceae; Fatty Acids; Gas Chromatography-Mass Spectrometry; Lethal Dose 50; Lipopolysaccharides; Mice; Myristic Acids; Rabbits; Water Microbiology
PubMed: 22830202
DOI: No ID Found -
Mikrobiologiia 2014A lipopolysaccharide (LPS) from Budvicia aquatica DRL 20186 was isolated, studied, and chemically identified. It was shown to be lowly toxic, but highly pyrogenic. Its...
A lipopolysaccharide (LPS) from Budvicia aquatica DRL 20186 was isolated, studied, and chemically identified. It was shown to be lowly toxic, but highly pyrogenic. Its fatty acid composition was similar to that of the LPS from other Enterobacteriaceae, with predominance of tetradecanoic (32.7%) and 3-hydroxytetradecanoic acids (23.8%). Hexadecenoic (20.4%), hexadecanoic (11.8%), and dodecanoic acids (8.4%) were also revealed. Double immunodiffusion in agar by the Ouchterlony method revealed antigenic activity of the B. aquatica DLR 20186 LPS in a homologous system. In cross reactions, however, it did not interact with the antisera to other @B. aquatica@ strains.
Topics: Animals; Carbohydrate Sequence; Enterobacteriaceae; Fatty Acids; Female; Lipopolysaccharides; Male; Mice; Molecular Sequence Data; Rabbits
PubMed: 25423734
DOI: No ID Found -
Mikrobiolohichnyi Zhurnal (Kiev,... 2011For the first time lipopolysaccharides (LPS) of 6 Budvicia aquatica strains--representatives of new Enterobacteriaceae species have been isolated. It was shown that the...
For the first time lipopolysaccharides (LPS) of 6 Budvicia aquatica strains--representatives of new Enterobacteriaceae species have been isolated. It was shown that the yield of LPS ranged from 0.9 to 7.0 % of cells dry weight. On the basis of monosaccharide composition LPS of tested strains may be referred to 3 groups. The serological studies indicated the immunochemical heterogeneity of B. aquatica species: LPS interacted only in homological system and showed no cross-reactivity with heterological antisera.
Topics: Enterobacteriaceae; Lipopolysaccharides; Monosaccharides
PubMed: 21809684
DOI: No ID Found -
Microbiology and Immunology Jul 2008Bacterial 16S ribosomal RNA genes (rDNA) were detected in blood samples from two healthy individuals by PCR under conditions involving 30 cycles that did not produce any...
Bacterial 16S ribosomal RNA genes (rDNA) were detected in blood samples from two healthy individuals by PCR under conditions involving 30 cycles that did not produce any visible products from negative control saline. Even from control samples, PCR involving 35-40 cycles yielded visible bands. Major clones detected in the blood samples, but not in control, were the Aquabacterium subgroup, Stenotrophomonas subgroup, Budvicia subgroup, Serratia subgroup, Bacillus subgroup and Flavobacteria subgroup. No clone was located within the bacteroides-clostridium-lactobacillus cluster, which is indigenous to gastrointestinal flora.
Topics: Bacteremia; Bacteria; Blood; DNA, Bacterial; DNA, Ribosomal; Humans; Polymerase Chain Reaction; RNA, Ribosomal, 16S
PubMed: 18667036
DOI: 10.1111/j.1348-0421.2008.00048.x -
Carbohydrate Research May 2012The following structure of the O-specific polysaccharide (O-antigen) of Budvicia aquatica 20186 was elucidated by sugar analysis along with 1D and 2D (1)H and (13)C NMR...
The following structure of the O-specific polysaccharide (O-antigen) of Budvicia aquatica 20186 was elucidated by sugar analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy: →4)-α-L-Rhap-(1→3)-α-D-Galp-(1→2)-α-Yerp-(1→3)-β-D-GalpNAc-(1→ where Yer stands for 3,6-dideoxy-4-C-[(S)-1-hydroxyethyl]-D-xylo-hexose (yersiniose A).
Topics: Carbohydrate Sequence; Enterobacteriaceae; Hexoses; Magnetic Resonance Spectroscopy; Molecular Sequence Data; O Antigens
PubMed: 22429774
DOI: 10.1016/j.carres.2012.02.017