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Current Microbiology Jan 2022Strain Marseille-P9829 was isolated from a bone sample collected from an open right fibula fracture from a 46-years old patient. Strain Marseille-P9829 (= CSUR...
Strain Marseille-P9829 was isolated from a bone sample collected from an open right fibula fracture from a 46-years old patient. Strain Marseille-P9829 (= CSUR P9829 = DSM 110695) was a Gram-negative, non-spore-forming and non-motile bacterium. This strain had a positive catalase activity but was oxidase-negative. The major fatty acids methyl esters were hexadecanoic acid (45.6%) and 9-hexadecenoic acid (28.4%). Matrix Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry analysis suggested that this strain belongs to the species Buttiauxella gaviniae. Since there were few reports of clinical infections with this species in humans, whole genome sequencing was performed and a polyphasic taxono-genomic approach was followed in order to verify the classification of strain Marseille-P9829. The 16S rRNA gene sequence BLAST against the NCBI database yielded the highest similarity of 99.8% with Buttiauxella agrestis, suggesting that strain Marseille-P9829 belongs to this species. However, genomic comparison by digital DNA-DNA hybridization showed that values between strain Marseille-P9829 and other validly published Buttiauxella species were all lower than 70%. Furthermore, all average nucleotide identities were lower than 95-96%. Therefore, these results confirmed that strain Marseille-P9829 belonged to a new Buttiauxella species for which we propose the name Buttiauxella massiliensis sp. nov., with strain Marseille-P9829 as type strain.
Topics: DNA, Bacterial; Fatty Acids; Genomics; Humans; Middle Aged; Nucleic Acid Hybridization; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 34982239
DOI: 10.1007/s00284-021-02714-3 -
Microbiology Resource Announcements May 2021We report here the complete genome sequence of DSM 9389, which harbors eight 16S rRNA genes classified into three types. The genome sequence of this strain showed a...
We report here the complete genome sequence of DSM 9389, which harbors eight 16S rRNA genes classified into three types. The genome sequence of this strain showed a high average nucleotide identity (97.3%) with that of the highly membrane vesicle-producing strain ATCC 33320.
PubMed: 33986092
DOI: 10.1128/MRA.00301-21 -
Archives of Virology Nov 2020We present here the results of the analysis of the complete genome sequence of a lytic bacteriophage, vB_ButM_GuL6, which is the first virus isolated from Buttiauxella....
We present here the results of the analysis of the complete genome sequence of a lytic bacteriophage, vB_ButM_GuL6, which is the first virus isolated from Buttiauxella. Electron microscopy revealed that vB_ButM_GuL6 belongs to the family Myoviridae, order Caudovirales. The genome of vB_ButM_GuL6 is a linear, circularly permuted 178,039-bp dsDNA molecule with a GC content of 43.4%. It has been predicted to contain 282 protein-coding genes and two tRNA genes, tRNA-Met and tRNA-Gly. Using bioinformatics approaches, 99 (36%) of the vB_ButM_GuL6 genes were assigned a putative function. Genome-wide comparisons and phylogenetic analysis indicated that vB_ButM_GuL6 represents a new species of the subfamily Tevenvirinae and is most closely related to Escherichia virus RB43. These phages, together with Cronobacter phages Miller, CfP1, and IME-CF2, likely form a new genus within the subfamily Tevenvirinae.
Topics: Crataegus; DNA, Viral; Enterobacteriaceae; Fruit; Genome, Viral; Lithuania; Microscopy, Electron; Myoviridae; Open Reading Frames; Phylogeny; Sequence Analysis, DNA; Viral Plaque Assay; Viral Proteins; Whole Genome Sequencing
PubMed: 32797340
DOI: 10.1007/s00705-020-04780-7 -
Archives of Microbiology Nov 2018A novel Gram-negative, rod-shaped, non-motile bacterium, designated C1B was isolated from a soil sample of a chrysanthemum plantation in Campinas, Brazil. Strain C1B...
A novel Gram-negative, rod-shaped, non-motile bacterium, designated C1B was isolated from a soil sample of a chrysanthemum plantation in Campinas, Brazil. Strain C1B formed white colonies on BHI medium, it produces acid from D-lactose, D-mannose, D-arabinose, but does not produce from D-adonitol, m-inositol, D-melibiose, D-raffinose and D-sorbitol and it is negative for lysine and ornithine decarboxylase, phenylalanine deaminase, and citrate. Phylogenetic analysis based on 16S rRNA and rpoB genes sequences showed that strain C1B has a similarity of 98.2 and 96.8% with different species of Buttiauxella genus. Major fatty acids were C, summed features 4 (C ω7c and iso-C 2OH), summed features 7 (C ω7c, C ω9t, and/or C ω12t), C cyclo, summed features 3 (iso-C I and C 3OH) and C. The mole percent of G+C was 49.6 mol%. Based on these results, a new species Buttiauxella chrysanthemi is proposed. The type strain is C1B (= CPQBA 1120/15 = CMRVSP5791).
Topics: Bacterial Typing Techniques; Base Composition; Brazil; Chrysanthemum; DNA, Bacterial; DNA-Directed RNA Polymerases; Enterobacteriaceae; Fatty Acids; Nucleic Acid Hybridization; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Soil Microbiology
PubMed: 29974159
DOI: 10.1007/s00203-018-1548-5 -
International Journal of Systematic... Jan 1996A total of 219 strains belonging to the genera Buttiauxella and Kluyvera were studied; 171 of these strains were isolated from mollusks, mainly snails and slugs,...
Emended description of Buttiauxella agrestis with recognition of six new species of Buttiauxella and two new species of Kluyvera: Buttiauxella ferragutiae sp. nov., Buttiauxella gaviniae sp. nov., Buttiauxella brennerae sp. nov., Buttiauxella izardii sp. nov., Buttiauxella noackiae sp. nov.,...
A total of 219 strains belonging to the genera Buttiauxella and Kluyvera were studied; 171 of these strains were isolated from mollusks, mainly snails and slugs, obtained from around the world. On the basis of DNA-DNA hybridization data, the strains were grouped into 11 genomospecies. A total of 44 phenotypic characters were used to differentiate the genera Buttiauxella and Kluyvera at the genus level and to identify genomospecies. There were significantly higher phenotypic probability distances between the genomospecies in the genus Battiauxella and the genomospecies in the genus Kluyvera than between the genomospecies in the same genus. Therefore, the existence of Buttiauxella and Kluyvera as different genera was confirmed. The existence of new species necessitated broadening the definitions of both genera. In two cases, Buttiauxella species could not be quantitatively differentiated biochemically, and several other pairs of species could be separated only by the results of one biochemical test. Nonetheless, combinations of several characteristics were used in differentiate all of the species with levels of certainly ranging from log 10.79 to log 57.77 (calculated as probability distances). The following new species are proposed: Buttiauxella ferragutiae (type strain, ATCC 51602 [DSM 9390]), Buttiauxella gaviniae (type strain, ATCC 51604 [DSM 9393]), Buttiauxella brennerae (type strain, ATCC 51605 [DSM 9396]), Buttiauxella izardii (type strain, ATCC 51606 [DSM 9397]), Buttiauxella noackiae (type strain, ATCC 51607 [DSM 9401]), Buttiauxella warmboldiae (type strain, ATCC 51608 [DSM 9404]), Kluyvera cochleae (type strain, ATCC 51609 [DSM 9406]), and Kluyvera georgiana (type strain, ATCC 51603 [DSM 9409]).
Topics: Animals; Bacterial Typing Techniques; DNA, Bacterial; Enterobacteriaceae; Humans; Intestines; Mollusca; Nucleic Acid Hybridization; Phenotype; Snails; Soil Microbiology; Terminology as Topic; Water Microbiology
PubMed: 11534554
DOI: 10.1099/00207713-46-1-50 -
Microbiology Resource Announcements Mar 2022We report the draft genome sequences of spp. strains that were isolated from water and gastropods. Three isolates show fluorescence in the Colilert system, indicating...
We report the draft genome sequences of spp. strains that were isolated from water and gastropods. Three isolates show fluorescence in the Colilert system, indicating unusual β-d-glucuronidase activity, and phylogenetic analyses suggest that they represent a novel species. Another strain, without β-d-glucuronidase activity, was assigned to the species Buttiauxella ferragutiae.
PubMed: 35234507
DOI: 10.1128/mra.00064-22 -
Molecules (Basel, Switzerland) Feb 20232,4,6-Trinitrotoluene (TNT) is an aromatic pollutant that is difficult to be degraded in the natural environment. The screening of efficient degrading bacteria for...
2,4,6-Trinitrotoluene (TNT) is an aromatic pollutant that is difficult to be degraded in the natural environment. The screening of efficient degrading bacteria for bioremediation of TNT has received much attention from scholars. In this paper, transcriptome analysis of the efficient degrading bacterium sp. S19-1 revealed that the monooxygenase gene () was significantly up-regulated during TNT degradation. S-Δ (absence of gene in S19-1 mutant) degraded TNT 1.66-fold less efficiently than strain S19-1 (from 71.2% to 42.9%), and E- mutant (-expressing strain) increased the efficiency of TNT degradation 1.33-fold (from 52.1% to 69.5%) for 9 h at 180 rpm at 27 °C in LB medium with 1.4 µg·mL TNT. We predicted the structure of BuMO and purified recombinant BuMO (rBuMO). Its specific activity was 1.81 µmol·min·mg protein at pH 7.5 and 35 °C. The results of gas chromatography mass spectrometry (GC-MS) analysis indicated that 4-amino-2,6-dinitrotoluene (ADNT) is a metabolite of TNT biodegradation. We speculate that MO is involved in catalysis in the bacterial degradation pathway of TNT in TNT-polluted environment.
Topics: Biodegradation, Environmental; Trinitrotoluene; Mixed Function Oxygenases; Escherichia coli
PubMed: 36838956
DOI: 10.3390/molecules28041969 -
Genome Announcements Oct 2014MI agar is routinely used for quantifying Escherichia coli in drinking water. A suspect E. coli colony isolated from a water sample was identified as Buttiauxella...
MI agar is routinely used for quantifying Escherichia coli in drinking water. A suspect E. coli colony isolated from a water sample was identified as Buttiauxella agrestis. The whole genome sequence of B. agrestis was determined to understand the genetic basis for its phenotypic resemblance to E. coli on MI agar.
PubMed: 25323724
DOI: 10.1128/genomeA.01060-14 -
Microbiology Resource Announcements Jul 2019We report here the draft genome sequence of sp. strain A111, isolated on the basis of bioconversion activity of the plant growth-regulating compound 2-azahypoxanthine...
We report here the draft genome sequence of sp. strain A111, isolated on the basis of bioconversion activity of the plant growth-regulating compound 2-azahypoxanthine to 2-aza-8-oxohypoxanthine. The genome contains 4,388 protein-coding sequences, including several genes possibly involved in the metabolism of the plant growth-regulating compound.
PubMed: 31320419
DOI: 10.1128/MRA.00664-19 -
Toxics Sep 2021Extensive use and disposal of 2,4,6-trinitrotoluene (TNT), a primary constituent of explosives, pollutes the environment and causes severe damage to human health....
Extensive use and disposal of 2,4,6-trinitrotoluene (TNT), a primary constituent of explosives, pollutes the environment and causes severe damage to human health. Complete mineralization of TNT via bacterial degradation has recently gained research interest as an effective method for the restoration of contaminated sites. Here, screening for TNT degradation by six selected bacteria revealed that sp. S19-1, possesses the strongest degrading ability. Moreover, (a gene encoding for protocatechuate 3,4-dioxygenase-P34O, a key enzyme in the β-ketoadipate pathway) was upregulated during TNT degradation. A knockout of in S19-1 to generate S-M1 mutant strain caused a marked reduction in TNT degradation efficiency compared to S19-1. Additionally, the EM1 mutant strain ( DH5α transfected with ) showed higher degradation efficiency than DH5α. Gas chromatography mass spectrometry (GC-MS) analysis of TNT degradation by S19-1 revealed 4-amino-2,6-dinitrotolune (ADNT) as the intermediate metabolite of TNT. Furthermore, the recombinant protein P34O (rP34O) expressed the activity of 2.46 µmol/min·mg. Our findings present the first report on the involvement of P34O in bacterial degradation of TNT and its metabolites, suggesting that P34O could catalyze downstream reactions in the TNT degradation pathway. In addition, the TNT-degrading ability of S19-1, a Gram-negative marine-derived bacterium, presents enormous potential for restoration of TNT-contaminated seas.
PubMed: 34678927
DOI: 10.3390/toxics9100231