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International Journal of Systematic... Jan 1996A total of 219 strains belonging to the genera Buttiauxella and Kluyvera were studied; 171 of these strains were isolated from mollusks, mainly snails and slugs,...
Emended description of Buttiauxella agrestis with recognition of six new species of Buttiauxella and two new species of Kluyvera: Buttiauxella ferragutiae sp. nov., Buttiauxella gaviniae sp. nov., Buttiauxella brennerae sp. nov., Buttiauxella izardii sp. nov., Buttiauxella noackiae sp. nov.,...
A total of 219 strains belonging to the genera Buttiauxella and Kluyvera were studied; 171 of these strains were isolated from mollusks, mainly snails and slugs, obtained from around the world. On the basis of DNA-DNA hybridization data, the strains were grouped into 11 genomospecies. A total of 44 phenotypic characters were used to differentiate the genera Buttiauxella and Kluyvera at the genus level and to identify genomospecies. There were significantly higher phenotypic probability distances between the genomospecies in the genus Battiauxella and the genomospecies in the genus Kluyvera than between the genomospecies in the same genus. Therefore, the existence of Buttiauxella and Kluyvera as different genera was confirmed. The existence of new species necessitated broadening the definitions of both genera. In two cases, Buttiauxella species could not be quantitatively differentiated biochemically, and several other pairs of species could be separated only by the results of one biochemical test. Nonetheless, combinations of several characteristics were used in differentiate all of the species with levels of certainly ranging from log 10.79 to log 57.77 (calculated as probability distances). The following new species are proposed: Buttiauxella ferragutiae (type strain, ATCC 51602 [DSM 9390]), Buttiauxella gaviniae (type strain, ATCC 51604 [DSM 9393]), Buttiauxella brennerae (type strain, ATCC 51605 [DSM 9396]), Buttiauxella izardii (type strain, ATCC 51606 [DSM 9397]), Buttiauxella noackiae (type strain, ATCC 51607 [DSM 9401]), Buttiauxella warmboldiae (type strain, ATCC 51608 [DSM 9404]), Kluyvera cochleae (type strain, ATCC 51609 [DSM 9406]), and Kluyvera georgiana (type strain, ATCC 51603 [DSM 9409]).
Topics: Animals; Bacterial Typing Techniques; DNA, Bacterial; Enterobacteriaceae; Humans; Intestines; Mollusca; Nucleic Acid Hybridization; Phenotype; Snails; Soil Microbiology; Terminology as Topic; Water Microbiology
PubMed: 11534554
DOI: 10.1099/00207713-46-1-50 -
Microbiology Resource Announcements Mar 2022We report the draft genome sequences of spp. strains that were isolated from water and gastropods. Three isolates show fluorescence in the Colilert system, indicating...
We report the draft genome sequences of spp. strains that were isolated from water and gastropods. Three isolates show fluorescence in the Colilert system, indicating unusual β-d-glucuronidase activity, and phylogenetic analyses suggest that they represent a novel species. Another strain, without β-d-glucuronidase activity, was assigned to the species Buttiauxella ferragutiae.
PubMed: 35234507
DOI: 10.1128/mra.00064-22 -
Polish Journal of Veterinary Sciences Sep 2018Wild ruminants are an interesting topic for research because only limited information exists regarding their microbiota. They could also be an environmental reservoir of...
Wild ruminants are an interesting topic for research because only limited information exists regarding their microbiota. They could also be an environmental reservoir of undesirable bacteria for other animals or humans. In this study faeces of the 21 free-living animals was sampled (9 Cervus elaphus-red deer, adult females, 12 Capreolus capreolus-roe deer, young females). They were culled by selective-reductive shooting during the winter season of 2014/2015 in the Strzałowo Forest District-Piska Primeval Forest (53° 36 min 43.56 sec N, 21° 30 min 58.68 sec E) in Poland. Buttiauxella sp. is a psychrotolerant, facultatively anaerobic, Gram-negative rod anaerobic bacte- rial species belonging to the Phylum Proteobacteria, Class Gammaproteobacteria, Order Entero- bacteriales, Family Enterobacteriacae and to Genus Buttiauxella. Buttiauxella sp. has never previ- ously been reported in wild ruminants. In this study, identification, antimicrobial profile and sensitivity to enterocins of Buttiauxella strains were studied as a contribution to the microbiota of wild animals, but also to extend knowledge regarding the antimicrobial spectrum of enterocins. Five strains were identified using the MALDI-TOF identification system (evaluation score value was up to 2.224) and allotted to the genus Buttiauxella including the species Buttiauxella gaviniae, B. ferragutiae, B. agrestis. Strains were DNase negative, and they hydrolysed esculin; fermentation of L-arabinose, D-mannitol and D-mannose was positive. Dulcitol, inositol reaction, urea and indol were negative. Buttiauxella strains did not form biofilm. They were resistant to at least one of the 13 antibiotics tested. B. agrestis 2/109/1 was resistant to amdinocillin, clindamycin and pen- icillin. However, Buttiauxella strains were sensitive to the enterocins used (inhibition activity ranged from 100 to 25 600 AU/ml).
Topics: Animals; Anti-Bacterial Agents; Deer; Drug Resistance, Bacterial; Enterobacteriaceae; Feces; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PubMed: 30468327
DOI: 10.24425/124288 -
International Journal of Systematic and... Apr 2024Two Gram-stain-negative bacterial strains, R39 and R73, were isolated from the rhizosphere soil of the selenium hyperaccumulator in China. Strain R39 transformed...
Two Gram-stain-negative bacterial strains, R39 and R73, were isolated from the rhizosphere soil of the selenium hyperaccumulator in China. Strain R39 transformed selenite into elemental and volatile selenium, whereas strain R73 transformed both selenate and selenite into elemental selenium. Phylogenetic and phylogenomic analyses indicated that strain R39 belonged to the genus , while strain R73 belonged to the genus . Strain R39 (genome size, 6.68 Mb; G+C content, 61.6 mol%) showed the closest relationship to LMG 26219 and LMG 3441, with average nucleotide identity (ANI) values of 83.6 and 83.4 %, respectively. Strain R73 (genome size, 5.22 Mb; G+C content, 50.3 mol%) was most closely related to ATCC 51602 with an ANI value of 86.4 %. Furthermore, strain A111 from the GenBank database was found to cluster with strain R73 within the genus through phylogenomic analyses. The ANI and digital DNA-DNA hybridization values between strains R73 and A111 were 97.5 and 80.0% respectively, indicating that they belong to the same species. Phenotypic characteristics also differentiated strain R39 and strain R73 from their closely related species. Based on the polyphasic analyses, strain R39 and strain R73 represent novel species of the genera and , respectively, for which the names sp. nov. (type strain R39=GDMCC 1.3843=JCM 36009) and sp. nov. (type strain R73=GDMCC 1.3636=JCM 35850) are proposed.
Topics: Fatty Acids; Sequence Analysis, DNA; Selenium; Cardamine; Phylogeny; Rhizosphere; Base Composition; DNA, Bacterial; Bacterial Typing Techniques; RNA, Ribosomal, 16S; Achromobacter; Selenious Acid
PubMed: 38619980
DOI: 10.1099/ijsem.0.006334