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BMC Infectious Diseases Jan 2022Data on antimicrobial resistance mechanisms are scanty for Cedecea spp., with very variable antibiotic resistance patterns documented. Here we report the first in vivo...
BACKGROUND
Data on antimicrobial resistance mechanisms are scanty for Cedecea spp., with very variable antibiotic resistance patterns documented. Here we report the first in vivo resistance evolution of a C. davisae clinical isolate in a patient with a complex hand trauma and provide insight in the resistance mechanism, leading to therapeutic implications for this pathogen.
CASE PRESENTATION
Cedecea davisae was isolated from a patient with hand trauma during a first surgical debridement. Six days after primary surgical treatment and under antimicrobial treatment with amoxicillin-clavulanic acid and later cefepime, follow up cultures yielded C. davisae which demonstrated a resistance development. The susceptible parental isolate and its resistant derivative were characterized by whole genome sequencing, ampC, ompC and ompF by RT- PCR. The resistant derivative demonstrated an A224G SNP in ampD, the transcriptional regulator of ampC, leading to a His75Arg change in the corresponding AmpD protein. AmpC transcription of the resistant derivative was 362-times higher than the susceptible isolate. Transcription levels of ompF and ompC were 8.5-fold and 1.3-fold lower, respectively, in the resistant derivative. Downregulation of OmpF putatively resulted from a mutation in the presumed promoter region upstream of the dusB-Fis operon, a proposed regulator for ompF.
CONCLUSIONS
This case demonstrates the in vivo resistance development of C. davisae within 7 days similar to that of the members of the Enterobacter cloacae complex. Our findings add valuable information for future therapeutic management of these opportunistic pathogens as they warrant the same empirical treatment as AmpC producers.
Topics: Anti-Bacterial Agents; Bacterial Proteins; Enterobacteriaceae; Humans; Microbial Sensitivity Tests; beta-Lactamases
PubMed: 34991516
DOI: 10.1186/s12879-021-07000-y -
Journal of Clinical Microbiology Oct 1986A case of bacteremia caused by Cedecea davisae is presented. This is the first reported case of bacteremia caused by this organism.
A case of bacteremia caused by Cedecea davisae is presented. This is the first reported case of bacteremia caused by this organism.
Topics: Aged; Catheters, Indwelling; Enterobacteriaceae; Enterobacteriaceae Infections; Female; Humans; Sepsis
PubMed: 3771758
DOI: 10.1128/jcm.24.4.675-676.1986 -
The American Journal of Case Reports 2013Female, 77.
PATIENT
Female, 77.
FINAL DIAGNOSIS
Bacteremia.
SYMPTOMS
Chills • diarrhea • fever • nausea.
MEDICATION
-
CLINICAL PROCEDURE
X-Ray • CBC • urine and blood cultur.
SPECIALTY
Infectious diseases.
OBJECTIVE
Rare disease.
BACKGROUND
Cedecea davisae is a gram negative, oxidase negative bacilli that include 5 species. In the medical literature there are very few reports that describe infections caused by different species of the Cedecea genus.
CASE REPORT
In this paper we report a fourth case of bacteremia in a 77 year-old patient with a chronic renal disease that was successfully treated with ceftazidim and ciprofloxacin. Additionally, we present a review of all the reported infections that were caused by C. davisae.
CONCLUSIONS
Five cases (not including our report) of Cedecea bacteremia were reported so far. Cedecea infections and particularly C. davisae infections can be difficult to treat due to the antibiotic resistance of the bacterium. Therefore we propose to consider treating C. davisae bacteremia with a combined antibiotic treatment until getting laboratory results for antibiotic-sensitivity tests.
PubMed: 23826472
DOI: 10.12659/AJCR.889285 -
Case Reports in Infectious Diseases 2012Chronic airway colonization and infection are the hallmark of cystic fibrosis (CF). Staphylococcus aureus, Pseudomonas aeruginosa, and Burkholderia cepacia are...
Chronic airway colonization and infection are the hallmark of cystic fibrosis (CF). Staphylococcus aureus, Pseudomonas aeruginosa, and Burkholderia cepacia are well-documented bacterial culprits in this chronic suppurative airway disease. Advanced molecular diagnostics have uncovered a possible role of a larger group of microorganisms in CF. Cedecea is a member of the family Enterobacteriaceae and is an emerging pathogen. We present a case of a polymicrobial healthcare-associated pneumonia in a CF patient caused by Cedecea davisae, among other bacteria.
PubMed: 23320209
DOI: 10.1155/2012/176864 -
Cureus Aug 2019Cedecea davisae is a gram-negative, non-sporulating motile rod-shaped bacteria of the Enterobacteriaceae family. It is an opportunistic pathogen in advanced-aged...
Cedecea davisae is a gram-negative, non-sporulating motile rod-shaped bacteria of the Enterobacteriaceae family. It is an opportunistic pathogen in advanced-aged patients with many comorbid diseases and the immunosuppressed. To the best of our knowledge, only 12 cases of C. davisae bacteremia have been reported in the literature. Here we discuss the 13th case of C. davisae bacteremia, which is the first reported case presenting as biliary sepsis. A 41-year-old female, on prednisone for minimal change disease, presented with nausea, vomiting, fever, and diarrhea. She had dry mucous membranes, scleral icterus, and elevated liver enzymes. Blood cultures revealed Cedecea davisae. She improved after management with broad-spectrum antibiotics. Further studies are needed to understand its role in the mode of transmission, the spectrum of infection, and treatment options. There is a need for physicians to be cognizant of emerging pathogens and address their antibiotic resistance profiles.
PubMed: 31579638
DOI: 10.7759/cureus.5298 -
Kulak Burun Bogaz Ihtisas Dergisi : KBB... 2015Atrophic rhinitis is a chronic inflammatory disease characterized by progressive atrophy of nasal mucosa. Cedecea davisae, a rare pathogen, is a new member of...
Atrophic rhinitis is a chronic inflammatory disease characterized by progressive atrophy of nasal mucosa. Cedecea davisae, a rare pathogen, is a new member of Enterobacteriaceae family. In this article, we report a patient with atrophic rhinitis whose culture test revealed Cedecea davisae. The patient was operated due to accompanying posterior ethmoid mucocele. Levofloxacin and nasal irrigation were administered for two months. Significant improvement was observed in patient's complaints and nasal signs at postoperative sixth month. In conclusion, Cedecea davisae has been thought to cause atrophic rhinitis and mucocele in this patient. Patient recovered with simple treatment. These bacteria should be kept in mind as a causative agent for atrophic rhinitis.
Topics: Enterobacteriaceae; Enterobacteriaceae Infections; Female; Humans; Middle Aged; Mucocele; Nasal Mucosa; Rhinitis, Atrophic; Tomography, X-Ray Computed
PubMed: 26211869
DOI: 10.5606/kbbihtisas.2015.93265 -
Applied and Environmental Microbiology Oct 1985The pyrazines responsible for the potatolike odor produced by some Serratia and Cedecea cultures were identified by gas-liquid chromatography (with flame ionization and...
The pyrazines responsible for the potatolike odor produced by some Serratia and Cedecea cultures were identified by gas-liquid chromatography (with flame ionization and thermoionic ionization detectors) and mass spectrometry. Alkylpyrazines were produced by the six strains studied irrespective of their odors. The major alkyl-methoxypyrazine produced by Cedecea davisae was 3-sec-butyl-2-methoxypyrazine, and that produced by odoriferous Serratia strains (S. rubidaea, S. odorifera, and S. ficaria) was 3-isopropyl-2-methoxy-5-methyl-pyrazine. Other pyrazines produced by some strains were 3-isopropyl-2-methoxypyrazine, 3-isobutyl-2-methoxypyrazine, 3-sec-butyl-2-methoxy-5-methylpyrazine, and 3-isobutyl-2-methoxy-6-methylpyrazine. Some of these pyrazines had not previously been found as natural products or to be produced by bacteria.
PubMed: 16346901
DOI: 10.1128/aem.50.4.1048-1051.1985 -
International Journal of Environmental... Nov 2021Most bacteria found in ticks are not pathogenic to humans but coexist as endosymbionts and may have effects on tick fitness and pathogen transmission. In this study, we...
Most bacteria found in ticks are not pathogenic to humans but coexist as endosymbionts and may have effects on tick fitness and pathogen transmission. In this study, we cultured and isolated 78 bacteria from 954 ticks collected in 7 sites of a Belgian peri-urban forest. Most isolated species were non-pathogenic environmental microorganisms, and were from the Firmicutes (69.23%), Actinobacteria (17.95%) and Proteobacteria (3.84%) phyla. One bacterium isolate was particularly noteworthy, , a rare opportunistic bacterium, naturally resistant to various antibiotics. It has never been isolated from ticks before and this isolated strain was resistant to ampicillin, cefoxitin and colistin. Although cultivable bacteria do not represent the complete tick microbiota, the sites presented variable bacterial compositions and diversities. This study is a first attempt to describe the culturable microbiota of ticks collected in Belgium. Further collections and analyses of ticks of different species, from various areas and using other bacterial identification methods would strengthen these results. However, they highlight the importance of ticks as potential sentinel for opportunistic bacteria of public health importance.
Topics: Animals; Bacteria; Belgium; Forests; Humans; Ixodes; Public Health
PubMed: 34831890
DOI: 10.3390/ijerph182212134 -
Indian Journal of Medical Microbiology 2020In the past years, saprophytic bacteria and candida have been emerging as potential human pathogens causing life-threatening infections in patients with cancer. This...
BACKGROUND
In the past years, saprophytic bacteria and candida have been emerging as potential human pathogens causing life-threatening infections in patients with cancer. This study was designed to determine the bacteria and candida species, causing bloodstream infections in cancer patients and the assessment of their susceptibility to antibiotics and biosynthesised silver nanoparticles.
MATERIALS AND METHODS
Ninety-seven microbial pathogens recovered from blood samples of cancer patients were included in the present study. The microbial isolates were collected in a duration period extending from December 2016 to July 2018 at National Cancer Institute, Cairo, Egypt. The clinical samples were collected using microbiological methods and were cultivated on MacConkey agar, blood agar media and Sabouraud dextrose agar media. The microbial isolates were identified using both standard microbiological methods and VITEK 2 compact automated system. The antibiotic resistance pattern was determined by the VITEK 2 compact automatic system and disk diffusion method, according to the Clinical and Laboratory Standards Institute. The characterisation of nanoparticles was carried out using ultraviolet spectroscopy and electron microscope. The antimicrobial activity of bio (AgNPs) was evaluated.
RESULTS
A total of 97 microbial isolates recovered from collected blood samples from cancer patients were included in the study. Pathogenic bacteria and Candida were represented by 74 isolates (76.22%) and 23 isolates (23.69), respectively. Among the 74 bacterial isolates, Escherichia coli constituted (27.81%), Klebsiella pneumoniae (24.72%), Acinetobacter baummannii (11.33%), Pseudomonas aeruginosa (4.12%), Enterobacter spp. (3.09%) and) Staphylococcus aureus (2.06%). Cedecea davisae (1.03%), Burkholderia cepacia (1.03%) and Pantoea agglomerans (1.03%). Among the 23 Candida isolates, Candida tropicalis constituted (9.27%), Candida albicans (5.15%), Candida glabrata (5.15%) and Candida krusei (4.12%) from the total microbial isolates. The antibiotic susceptibility results revealed that amikacin and gentamycin were the most effective antibiotics against Gram-negative bacteria, while vancomycin and linezolid were most effective against S. aureus. Caspofungin was the most effective against candida species. The obtained stable biosynthesised silver nanoparticles ranged in size from 10 nm to 100 nm and were mostly spherical in shape. These biosilver nanoparticles showed the highest antimicrobial activity against most of the microbial isolates (bacteria and Candida). The in vitro cytotoxicity of biosynthesised AgNPs on HeLa cell lines revealed a dose-dependent potential. The IC50 value of AgNPs was found 6 and 5.6 μg/ml, respectively.
CONCLUSION
The present study revealed a significant distribution of multidrug-resistant microbes, which may increase the burden of healthcare to prevent infections in cancer patients. Biosilver nanoparticles exhibit antimicrobial activity against multidrug-resistant microbes and could be considered as effective agents against these strains.
Topics: Bacteremia; Bacteria; Candida; Candidemia; Drug Resistance, Multiple; Female; Humans; Male; Metal Nanoparticles; Neoplasms; Silver
PubMed: 33154249
DOI: 10.4103/ijmm.IJMM_20_299 -
Antimicrobial Agents and Chemotherapy Nov 2014A Cedecea davisae isolate, which was intermediate or resistant to third-generation cephalosporins and carbapenems, was recovered from a urine sample. Susceptibility...
A Cedecea davisae isolate, which was intermediate or resistant to third-generation cephalosporins and carbapenems, was recovered from a urine sample. Susceptibility testing, isoelectric focusing, and analysis of outer membrane proteins showed that AmpC β-lactamase expression combined with porin deficiency accounted for the carbapenem resistance. A cloning experiment followed by phenotypic and enzymatic characterization identified a novel class C enzyme that was phylogenetically and biochemically close to the chromosome-borne β-lactamases of the genera Enterobacter and Citrobacter.
Topics: Aged; Amino Acid Sequence; Bacterial Outer Membrane Proteins; Bacterial Proteins; Carbapenems; Drug Resistance, Bacterial; Enterobacteriaceae; Female; Humans; Microbial Sensitivity Tests; Molecular Sequence Data; Phylogeny; Porins; beta-Lactamases
PubMed: 25136020
DOI: 10.1128/AAC.03237-14