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Advanced Science (Weinheim,... May 2020Optical metasurfaces enable novel ways to locally manipulate light's amplitude, phase, and polarization, underpinning a newly viable technology for applications, such as...
Optical metasurfaces enable novel ways to locally manipulate light's amplitude, phase, and polarization, underpinning a newly viable technology for applications, such as high-density optical storage, holography, and displays. Here, a high-security-level platform enabled by centimeter-scale plasmonic metasurfaces with full-color, high-purity, and enhanced-information-capacity properties is proposed. Multiple types of independent information can be embedded into a single metamark using full parameters of light, including amplitude, phase, and polarization. Under incoherent white light, the metamark appears as a polarization- and angle-encoded full-color image with flexibly controlled hue, saturation, and brightness, while switching to multiwavelength holograms under coherent laser illumination. More importantly, for actual applications, the extremely shallow functional layer makes such centimeter-scale plasmonic metamarks suitable for cost-effective mass production processes. Considering these superior performances of the presented multifunctional plasmonic metasurfaces, this work may find wide applications in anticounterfeiting, information security, high-density optical storage, and so forth.
PubMed: 32440472
DOI: 10.1002/advs.201903156 -
Nature Apr 2017The ability to visualize directly a large number of distinct molecular species inside cells is increasingly essential for understanding complex systems and processes....
The ability to visualize directly a large number of distinct molecular species inside cells is increasingly essential for understanding complex systems and processes. Even though existing methods have successfully been used to explore structure-function relationships in nervous systems, to profile RNA in situ, to reveal the heterogeneity of tumour microenvironments and to study dynamic macromolecular assembly, it remains challenging to image many species with high selectivity and sensitivity under biological conditions. For instance, fluorescence microscopy faces a 'colour barrier', owing to the intrinsically broad (about 1,500 inverse centimetres) and featureless nature of fluorescence spectra that limits the number of resolvable colours to two to five (or seven to nine if using complicated instrumentation and analysis). Spontaneous Raman microscopy probes vibrational transitions with much narrower resonances (peak width of about 10 inverse centimetres) and so does not suffer from this problem, but weak signals make many bio-imaging applications impossible. Although surface-enhanced Raman scattering offers high sensitivity and multiplicity, it cannot be readily used to image specific molecular targets quantitatively inside live cells. Here we use stimulated Raman scattering under electronic pre-resonance conditions to image target molecules inside living cells with very high vibrational selectivity and sensitivity (down to 250 nanomolar with a time constant of 1 millisecond). We create a palette of triple-bond-conjugated near-infrared dyes that each displays a single peak in the cell-silent Raman spectral window; when combined with available fluorescent probes, this palette provides 24 resolvable colours, with the potential for further expansion. Proof-of-principle experiments on neuronal co-cultures and brain tissues reveal cell-type-dependent heterogeneities in DNA and protein metabolism under physiological and pathological conditions, underscoring the potential of this 24-colour (super-multiplex) optical imaging approach for elucidating intricate interactions in complex biological systems.
Topics: Animals; Brain; Cell Line; Cell Survival; Coculture Techniques; Color; Coloring Agents; DNA; Electrons; Fluorescent Dyes; Humans; Infrared Rays; Mice; Molecular Imaging; Neurons; Organ Specificity; Proteins; Spectrum Analysis, Raman; Vibration
PubMed: 28424513
DOI: 10.1038/nature22051 -
Philosophical Transactions. Series A,... Oct 2022Marginal ice zones (MIZs) are qualitatively distinct sea-ice-covered areas that play a critical role in the interaction between the polar oceans and the broader Earth... (Review)
Review
Marginal ice zones (MIZs) are qualitatively distinct sea-ice-covered areas that play a critical role in the interaction between the polar oceans and the broader Earth system. MIZ regions have high spatial and temporal variability in oceanic, atmospheric and ecological conditions. The salient qualitative feature of MIZs is their composition as a mosaic of individual floes that range in horizontal extent from centimetres to tens of kilometres. Thus the floe size distribution (FSD) can be used to quantitatively identify and describe them. Here, the history of FSD observations and theory, and the processes (particularly the impact of ocean waves) that determine floe sizes and size distribution, are reviewed. Coupled wave-FSD feedbacks are explored using a stochastic model for thermodynamic wave-sea-ice interactions in the MIZ, and some of the key open questions in this rapidly growing field are discussed. This article is part of the theme issue 'Theory, modelling and observations of marginal ice zone dynamics: multidisciplinary perspectives and outlooks'.
Topics: Feedback; Ice Cover; Oceans and Seas
PubMed: 36088924
DOI: 10.1098/rsta.2021.0252