Did you mean: clostridium cadaveric
-
Lipids Aug 2019The lipidomes of Clostridium fallax and Clostridium cadaveris were studied using thin-layer chromatography (TLC) and normal phase liquid chromatography/mass spectrometry...
The lipidomes of Clostridium fallax and Clostridium cadaveris were studied using thin-layer chromatography (TLC) and normal phase liquid chromatography/mass spectrometry (NPLC/MS). Both species contain diradylglycerol (DRG), monohexosyldiradylglycerol (MHDRG), monohexosyl monoacylglycerol (MHMAG), phosphatidylglycerol (PtdGro), and phosphatidylethanolamine (PtdEtn). DRG, MHDRG, PtdEtn, and PtdGro are present in both diacyl and alk-1-enyl acyl (plasmalogen) forms. Both species contain cardiolipin (Ptd Gro), which is present in tetraacyl, monoalkenyl-triacyl, and dialkenyl-diacyl forms. Both species contain small amounts of phosphatidylcholine (PtdCho). The presence of octadecadienoic (18:2) acyl chains in some PtdCho species indicates that they arise from the medium because no 18:2 is seen in the other lipids and clostridia generally lack the capacity to synthesize polyunsaturated fatty acids. The major lipidomic differences between these two species are that C. fallax contains a glycerolacetal of plasmenylethanolamine while C. cadaveris contains an ethanolamine-phosphate-modified diacylglycerol. The significance of these lipid compositions is discussed.
Topics: Chromatography, Liquid; Clostridium; Lipidomics; Lipids; Mass Spectrometry; Molecular Structure
PubMed: 31368115
DOI: 10.1002/lipd.12181 -
Journal of Microbiology and... Apr 2020A quantitative real-time polymerase chain reaction (QPCR) was applied to estimate biokinetic coefficients of and , which utilize protein as carbon source. Experimental...
A quantitative real-time polymerase chain reaction (QPCR) was applied to estimate biokinetic coefficients of and , which utilize protein as carbon source. Experimental data of changes in peptone concentration and 16S rRNA gene copy numbers of and were fitted to model. The fourth-order Runge-Kutta approximation with non-linear least squared analysis was employed to solve the ordinary differential equations to estimate biokinetic coefficients. The maximum specific growth rate (), half saturation concentration (), growth yield (), and decay coefficient () of and were 0.73 ± 0.05 and 1.35 ± 0.32 h, 6.07 ± 1.52 and 5.67 ± 1.53 g/L, 2.25 ± 0.75 × 10 and 7.92 ± 3.71 × 10 copies/g, 0.002 ± 0.003 and 0.002 ± 0.001 h, respectively. The theoretical specific growth rate of always exceeded than that of at peptone concentration higher than 3.62 g/L. When the influent peptone concentration was 5.0 g/L, the concentration of C.cadaveris gradually decreased to the steady value of 2.9 × 10 copies/mL at 4 hours HRT, which indicates 67.1% of the initial population reduction, but the wash out occurred at 1.9 and 3.2 hours HRTs. The 16S rRNA gene copy numbers of gradually decreased to steady values ranging from 1.1 × 10 to 2.9 × 10 copies/mL. species was predicted to wash out at an HRT of 1.6 h.
Topics: Batch Cell Culture Techniques; Biodegradation, Environmental; Bioreactors; Clostridium; Kinetics; Peptones; Proteins; RNA, Ribosomal, 16S
PubMed: 31986562
DOI: 10.4014/jmb.1908.08054 -
Genome Biology and Evolution Oct 2017Clostridium species (particularly Clostridium difficile, Clostridium botulinum, Clostridium tetani and Clostridium perfringens) are associated with a range of human and...
Clostridium species (particularly Clostridium difficile, Clostridium botulinum, Clostridium tetani and Clostridium perfringens) are associated with a range of human and animal diseases. Several other species including Clostridium tertium, Clostridium cadaveris, and Clostridium paraputrificum have also been linked with sporadic human infections, however there is very limited, or in some cases, no genomic information publicly available. Thus, we isolated one C. tertium strain, one C. cadaveris strain and three C. paraputrificum strains from preterm infants residing within neonatal intensive care units and performed Whole Genome Sequencing (WGS) using Illumina HiSeq. In this report, we announce the open availability of the draft genomes: C. tertium LH009, C. cadaveris LH052, C. paraputrificum LH025, C. paraputrificum LH058, and C. paraputrificum LH141. These genomes were checked for contamination in silico to ensure purity, and we confirmed species identity and phylogeny using both 16S rRNA gene sequences (from PCR and in silico) and WGS-based approaches. Average Nucleotide Identity (ANI) was used to differentiate genomes from their closest relatives to further confirm speciation boundaries. We also analysed the genomes for virulence-related factors and antimicrobial resistance genes, and detected presence of tetracycline and methicillin resistance, and potentially harmful enzymes, including multiple phospholipases and toxins. The availability of genomic data in open databases, in tandem with our initial insights into the genomic content and virulence traits of these pathogenic Clostridium species, should enable the scientific community to further investigate the disease-causing mechanisms of these bacteria with a view to enhancing clinical diagnosis and treatment.
Topics: Clostridium; Clostridium tertium; Feces; Genome, Bacterial; Humans; Infant, Newborn; Infant, Premature; Intensive Care Units, Neonatal; Phylogeny
PubMed: 29044436
DOI: 10.1093/gbe/evx210 -
Infection and Drug Resistance 2021Bacteremia caused by is an extremely rare infection that accounts for 0.5-2% of all positive blood cultures. is an opportunistic agent that is highly lethal in...
BACKGROUND
Bacteremia caused by is an extremely rare infection that accounts for 0.5-2% of all positive blood cultures. is an opportunistic agent that is highly lethal in immunocompromised or epithelial barrier disruption hosts.
CASE PRESENTATION
A 74-year-old woman was admitted to our hospital with a high fever. The patient was diagnosed with an abdominal infection after a bowel invasion and recurrence of an ovarian tumor after surgery. Blood sample culturing yielded transparent, smooth, moist, slightly raised colonies without a hemolytic ring. was identified by gram staining and MALDI-TOF MS. 16S rRNA gene sequencing confirmed a sequence homologous to in the GenBank database. Drug susceptibility testing showed that was sensitive to cefoxitin, clindamycin, imipenem, meropenem, metronidazole, and vancomycin. After treatment, the patient's body temperature was normal and she was discharged from the hospital.
CONCLUSION
This is the first case report of bacteremia caused by in China and also the first report of identifying in clinical specimens by MALDI-TOF MS. This case study could increase the awareness of these infections in China.
PubMed: 34955643
DOI: 10.2147/IDR.S344062 -
BMC Genomics Sep 2021Soil bacteria are a major source of specialized metabolites including antimicrobial compounds. Yet, one of the most diverse genera of bacteria ubiquitously present in...
BACKGROUND
Soil bacteria are a major source of specialized metabolites including antimicrobial compounds. Yet, one of the most diverse genera of bacteria ubiquitously present in soil, Clostridium, has been largely overlooked in bioactive compound discovery. As Clostridium spp. thrive in extreme environments with their metabolic mechanisms adapted to the harsh conditions, they are likely to synthesize molecules with unknown structures, properties, and functions. Therefore, their potential to synthesize small molecules with biological activities should be of great interest in the search for novel antimicrobial compounds. The current study focused on investigating the antimicrobial potential of four soil Clostridium isolates, FS01, FS2.2 FS03, and FS04, using a genome-led approach, validated by culture-based methods.
RESULTS
Conditioned/spent media from all four Clostridium isolates showed varying levels of antimicrobial activity against indicator microorganism; all four isolates significantly inhibited the growth of Pseudomonas aeruginosa. FS01, FS2.2, and FS04 were active against Bacillus mycoides and FS03 reduced the growth of Bacillus cereus. Phylogenetic analysis together with DNA-DNA hybridization (dDDH), average nucleotide identity (ANI), and functional genome distribution (FGD) analyses confirmed that FS01, FS2.2, and FS04 belong to the species Paraclostridium bifermentans, Clostridium cadaveris, and Clostridium senegalense respectively, while FS03 may represent a novel species of the genus Clostridium. Bioinformatics analysis using antiSMASH 5.0 predicted the presence of eight biosynthetic gene clusters (BGCs) encoding for the synthesis of ribosomally synthesized post-translationally modified peptides (RiPPs) and non-ribosomal peptides (NRPs) in four genomes. All predicted BGCs showed no similarity with any known BGCs suggesting novelty of the molecules from those predicted gene clusters. In addition, the analysis of genomes for putative virulence factors revealed the presence of four putative Clostridium toxin related genes in FS01 and FS2.2 genomes. No genes associated with the main Clostridium toxins were identified in the FS03 and FS04 genomes.
CONCLUSIONS
The presence of BGCs encoding for uncharacterized RiPPs and NRPSs in the genomes of antagonistic Clostridium spp. isolated from farm soil indicated their potential to produce novel secondary metabolites. This study serves as a basis for the identification and characterization of potent antimicrobials from these soil Clostridium spp. and expands the current knowledge base, encouraging future research into bioactive compound production in members of the genus Clostridium.
Topics: Anti-Infective Agents; Bacillus; Clostridium; Phylogeny; Soil
PubMed: 34548019
DOI: 10.1186/s12864-021-08005-2 -
Microbiology Resource Announcements Mar 2022We report the complete genome sequence of Clostridium cadaveris IFB3C5, a strain isolated from the resected tumor of a treatment naive colorectal cancer patient. This...
We report the complete genome sequence of Clostridium cadaveris IFB3C5, a strain isolated from the resected tumor of a treatment naive colorectal cancer patient. This genome is comprised of a singular chromosome of approximately 3.63 Mbp in length, contains two plasmids, and has an overall mean GC content of 31.7%.
PubMed: 35234492
DOI: 10.1128/mra.01135-21 -
Oxford Medical Case Reports Jan 2023(), a strict anaerobic gram-positive rod, is rarely reported in clinical specimens. Since its detection in 1899, it has always been linked to the decay of dead bodies....
(), a strict anaerobic gram-positive rod, is rarely reported in clinical specimens. Since its detection in 1899, it has always been linked to the decay of dead bodies. is considered non-pathogenic to humans, however infrequently it can cause severe infections including bacteremia. The latter was typically associated with gastro-intestinal pathologies. We report the first case of invasive infection at Sultanate Oman. The source was most probably an infected decubitus ulcer.
PubMed: 36694598
DOI: 10.1093/omcr/omac146 -
Journal of Lipid Research May 2017Members of the gastrointestinal microbiota are known to convert glucocorticoids to androstanes, which are subsequently converted to potent androgens by other members of...
Members of the gastrointestinal microbiota are known to convert glucocorticoids to androstanes, which are subsequently converted to potent androgens by other members of the gut microbiota or host tissues. and have previously been reported for steroid-17,20-desmolase and 20β-hydroxysteroid dehydrogenase (HSDH) activities that are responsible for androstane formation from cortisol; however, the genes encoding these enzymes have yet to be reported. In this work, we identified and located a gene encoding 20β-HSDH in both and The 20β-HSDH of was heterologously overexpressed and purified from The enzyme was determined to be a homotetramer with subunit molecular mass of 33.8 ± 3.7 kDa. The r20β-HSDH displayed pH optimum in the reductive direction at pH 9.0 and in the oxidative direction at pH 7.0-7.5 with (20β-dihydro)cortisol and NAD(H) as substrates. Cortisol is the preferred substrate with , 0.80 ± 0.06 μM; , 30.36 ± 1.97 μmol·min; , 607 ± 39 μmol·μM·min; / , 760 ± 7.67. Phylogenetic analysis of the 20β-HSDH from suggested that the 20β-HSDH is found in several , one of which was shown to express 20β-HSDH activity. Notably, we also identified a novel steroid-17,20-desmolase-elaborating bacterium, , a normal inhabitant of the urinary tract.
Topics: Anaerobiosis; Clostridiaceae; Gene Expression Regulation, Bacterial; Hydroxysteroid Dehydrogenases; Intestines; Operon; Phylogeny; Steroids
PubMed: 28314858
DOI: 10.1194/jlr.M074914 -
Canadian Journal of Veterinary Research... Apr 1992Four groups of two ponies, free of fecal Salmonella and Clostridium cadaveris, were treated as follows: Group A, control group; B, single nasogastrically administered...
Four groups of two ponies, free of fecal Salmonella and Clostridium cadaveris, were treated as follows: Group A, control group; B, single nasogastrically administered dose of lincomycin (25 mg/kg) followed 48 h later by 3 L of C. cadaveris (10(9) organisms/mL); C, the same dose of lincomycin as group B; D, the same dose of C. cadaveris as group B on each of three occasions at 12 h intervals. Groups A and D remained healthy, but groups B and C developed severe colitis 48-56 h (B) or 72 h (C) after administration of lincomycin. Three ponies were euthanized and one in group B died. Clostridium cadaveris was isolated at about 10(6)/mL of colonic contents from these ponies, but one pony in group B also yielded Salmonella typhimurium from the colon. Subsequent challenge of group A ponies (3 L of C. cadaveris 10(9)/mL, three times at 12 h intervals) did not produce colitis. Nasogastric administration of lincomycin (25 mg/kg) to group A and D ponies, 20 days after administration of C. cadaveris, resulted in severe colitis in all ponies within 48-72 h. Salmonella agona was isolated from the colonic contents of one pony and C. cadaveris (10(6)/mL) from all four ponies. Clostridium cadaveris was not isolated from the colonic content of 45 healthy horses examined immediately after death. These studies confirm the potential for lincomycin to induce severe enterocolitis in ponies and implicate C. cadaveris further as a cause of "idiopathic colitis" in ponies.
Topics: Animals; Clostridium; Disease Models, Animal; Enterocolitis, Pseudomembranous; Horse Diseases; Horses; Lincomycin; Salmonella
PubMed: 1591660
DOI: No ID Found -
Journal of Bone and Joint Infection 2020, named following its identification in human corpses, is an unusual pathogen. We report the first case of osteomyelitis. This case highlights the importance of deep...
, named following its identification in human corpses, is an unusual pathogen. We report the first case of osteomyelitis. This case highlights the importance of deep tissue sampling and appropriate culture to correctly identify causative pathogens and guide targeted antimicrobial therapy in difficult-to-treat infections like chronic osteomyelitis.
PubMed: 32455100
DOI: 10.7150/jbji.43801