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Frontiers in Microbiology 2022The chlorpyrifos-mineralizing rice root endophyte sp. HSTU-ASh6 strain was identified, which enormously enhanced the growth of tomato plant under epiphytic conditions....
The chlorpyrifos-mineralizing rice root endophyte sp. HSTU-ASh6 strain was identified, which enormously enhanced the growth of tomato plant under epiphytic conditions. The strain solubilizes phosphate and grew in nitrogen-free Jensen's medium. It secreted indole acetic acid (IAA; 4.8 mg/mL) and ACC deaminase (0.0076 μg/mL/h) and hydrolyzed chlorpyrifos phosphodiester bonds into 3,5,6-trichloro-2-pyridinol and diethyl methyl-monophosphate, which was confirmed by Gas Chromatography - Tandem Mass Spectrometry (GC-MS/MS) analysis. and (ANI, DDH, housekeeping genes and whole genome phylogenetic tree, and genome comparison) analyses confirmed that the strain belonged to a new species of . The annotated genome of strain HSTU-ASh6 revealed a sets of nitrogen-fixing, siderophore, S, and IAA producing, stress tolerance, phosphate metabolizing, and pesticide-degrading genes. The 3D structure of 28 potential model proteins that can degrade pesticides was validated, and virtual screening using 105 different pesticides revealed that the proteins exhibit strong catalytic interaction with organophosphorus pesticides. Selected docked complexes such as α/β hydrolase-crotoxyphos, carboxylesterase-coumaphos, α/β hydrolase-cypermethrin, α/β hydrolase-diazinon, and amidohydrolase-chlorpyrifos meet their catalytic triads in visualization, which showed stability in molecular dynamics simulation up to 100 ns. The foliar application of sp. strain HSTU-ASh6 on tomato plants significantly improved their growth and development at vegetative and reproductive stages in fields, resulting in fresh weight and dry weight was 1.8-2.0-fold and 1.3-1.6-fold higher in where urea application was cut by 70%, respectively. Therefore, the newly discovered chlorpyrifos-degrading species sp. HSTU-ASh6 could be used as a smart biofertilizer component for sustainable tomato cultivation.
PubMed: 36523825
DOI: 10.3389/fmicb.2022.1060554 -
Quantitative studies of the effect of antagonists on the acute toxicity of organophosphates in rats.British Journal of Pharmacology Sep 19701. The subcutaneous acute toxicities of the vinyl phosphate pesticides monocrotophos, dicrotophos, chlorfenvinphos, crotoxyphos, dichlorvos, mevinphos, and of the...
1. The subcutaneous acute toxicities of the vinyl phosphate pesticides monocrotophos, dicrotophos, chlorfenvinphos, crotoxyphos, dichlorvos, mevinphos, and of the experimental compounds SD 4455 (cis-2-carboxy-1-methylvinyl dimethylphosphate) and SD 7779 (cis-2-(1-phenylethoxy) carbonyl-1-methylvinyl diethylphosphate) have been determined in female rats.2. The effects on the log dose-probit mortality curves to the vinylphosphates of the therapeutic subcutaneous administration of methylatropine, atropine, N-methylpyridinium-2-aldoxime methanesulphonate and obidoxime have been studied.3. Elevation of the LD50 values by the therapeutic regimens was shown to be an unsatisfactory measure of therapeutic efficiency, while reduction of the effect of a maximally lethal dose (LD90) to less than that of a minimally lethal dose (LD10) provided a better quantitative measure of therapeutic efficiency.4. The combination of atropine sulphate (50 mumol base/kg) with obidoxime (250 mumol/kg) was found to be generally the most effective of the antidotal regimens.
Topics: Alkenes; Animals; Antidotes; Atropine; Drug Synergism; Female; Oximes; Pesticides; Phosphoric Acids; Pyridinium Compounds; Rats; Sulfonic Acids
PubMed: 5487002
DOI: 10.1111/j.1476-5381.1970.tb10617.x