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TheScientificWorldJournal 2014Heterotrophic nitrifiers synthesize nitrogenous gasses when nitrifying ammonium ion. A Cupriavidus pauculus, previously thought an Alcaligenes sp. and noted as an active...
Heterotrophic nitrifiers synthesize nitrogenous gasses when nitrifying ammonium ion. A Cupriavidus pauculus, previously thought an Alcaligenes sp. and noted as an active heterotrophic nitrifier-denitrifier, was examined for its ability to produce nitrogen gas (N2) and nitrous oxide (N2O) while heterotrophically nitrifying the organic substrate pyruvic oxime [CH3-C(NOH)-COOH]. Neither N2 nor N2O were produced. Nucleotide and phylogenetic analyses indicated that the organism is a member of a genus (Cupriavidus) known for its resistance to metals and its metabolism of xenobiotics. The microbe (a Cupriavidus pauculus designated as C. pauculus strain UM1) was examined for its ability to perform heterotrophic nitrification in the presence of Cu(2+) and Ni(2+) and to metabolize the xenobiotic phenol. The bacterium heterotrophically nitrified well when either 1 mM Cu(2+) or 0.5 mM Ni(2+) was present in either enriched or minimal medium. The organism also used phenol as a sole carbon source in either the presence or absence of 1 mM Cu(2+) or 0.5 mM Ni(2+). The ability of this isolate to perform a number of different metabolisms, its noteworthy resistance to copper and nickel, and its potential use as a bioremediation agent are discussed.
Topics: Copper; Cupriavidus; Denitrification; Heterotrophic Processes; Molecular Sequence Data; Nickel; Nitrification; Phylogeny; Propionates; RNA, Ribosomal, 16S
PubMed: 25580463
DOI: 10.1155/2014/901702 -
Journal of Medical Microbiology Feb 2022is historically found in soil and water but has more recently been reported to cause human infection and death. Hospital sink traps can serve as a niche for bacterial...
is historically found in soil and water but has more recently been reported to cause human infection and death. Hospital sink traps can serve as a niche for bacterial persistence and a platform for horizontal gene transfer, with evidence of dissemination of pathogens in hospital plumbing systems driving nosocomial infection. This paper presents the first strain isolated from a hospital sink trap. There are only six genome assemblies available on NCBI for ; two of these are PacBio/Illumina hybrids. This paper presents the first ONT/Illumina hybrid assembly, with five contigs. The other assemblies available consist of 37, 38, 111 and 227 contigs. This paper also presents data on biofilm formation and lethal dose in ; there is little published information describing these aspects of virulence. The aims were to identify the isolate found in a hospital sink trap, characterize its genome, and assess whether it could pose a risk to human health. The genome was sequenced, and a hybrid assembly of short and long reads produced. Antimicrobial susceptibility was determined by the broth microdilution method. Virulence was assessed by measuring biofilm formation compared to and lethality in larvae. The isolate was confirmed to be a strain of , with a 6.8 Mb genome consisting of 6468 coding sequences and an overall G+C content of 63.9 mol%. The genome was found to contain 12 antibiotic resistance genes, 8 virulence factor genes and 33 metal resistance genes. The isolate can be categorized as resistant to meropenem, amoxicillin, amikacin, gentamicin and colistin, but susceptible to cefotaxime, cefepime, imipenem and ciprofloxacin. Clear biofilm formation was seen in all conditions over 72 h and exceeded that of when measured at 37 °C in R2A broth. Lethality in larvae over 48 h was relatively low. The appearance of a multidrug-resistant strain of in a known pathogen reservoir within a clinical setting should be considered concerning. Further work should be completed to compare biofilm formation and virulence between clinical and environmental strains, to determine how easily environmental strains may establish human infection. Infection control teams and clinicians should be aware of the emerging nature of this pathogen and further work is needed to minimize the impact of contaminated hospital plumbing systems on patient outcomes.
Topics: Animals; Anti-Bacterial Agents; Cupriavidus; Drug Resistance, Multiple, Bacterial; Equipment Contamination; Genome, Bacterial; Hospitals; Humans; Moths; Water Supply
PubMed: 35113779
DOI: 10.1099/jmm.0.001501 -
Andes Pediatrica : Revista Chilena de... Apr 2022Catheter-related bacteriemia by Cupriavidus spp. is a rare condition with very few cases reported in the literature. Most of them occurred in immunocompromised patients.
UNLABELLED
Catheter-related bacteriemia by Cupriavidus spp. is a rare condition with very few cases reported in the literature. Most of them occurred in immunocompromised patients.
OBJECTIVE
To report a case of recurrent catheter-related bacteriemia by Cupriavidus pauculus in an immunocompromised infant in order to analyze possible therapeutic options, especially in relation to the need or not for central venous catheter (CVC) removal.
CLINICAL CASE
22-month-old infant with B-cell acute lymphoblas tic leukemia (ALL) in reinduction phase, CVC carrier. He presented to the Emergency Room with fever without focus on examination. Blood tests were performed (without increase of acute phase reactants) and differential blood cultures (peripheral and CVC). He was hospitalized and empirical antibiotic therapy was started with intravenous fourth-generation cephalosporin (cefepime). After 24 hours, blood cultures were positive for Cupriavidus pauculus, growing first in the CVC culture. We maintained cefepime, adding catheter lock therapy with ciprofloxacin. Afterward, the infection was resolved, allowing us to keep the CVC. Seven months later, in the context of fever, Cupriavidus pauculus was again identified in CVC blood culture. We decided this time to remove the catheter, in addition to the administration of intravenous cefepime. The patient has not presented new episodes nine months after de removal of the CVC.
CONCLUSION
Catheter-related bacteremia by Cupriavidus is a rare condition in children that usually occurs in immunocompromised patients. Catheter lock therapy associated with systemic antibiotics could be a safe option in patients with difficult CVC re moval. However, if persistent colonization of the CVC is suspected, it may be necessary to remove it.
Topics: Bacteremia; Cefepime; Central Venous Catheters; Child; Cupriavidus; Gram-Negative Facultatively Anaerobic Rods; Humans; Infant; Male
PubMed: 35735306
DOI: 10.32641/andespediatr.v93i2.3921 -
Journal of Critical Care Medicine... Oct 2016is a rarely isolated non-fermentative, aerobic bacillus, which occasionally causes severe human infections, especially in immunocompromised patients. Strains have been...
INTRODUCTION
is a rarely isolated non-fermentative, aerobic bacillus, which occasionally causes severe human infections, especially in immunocompromised patients. Strains have been isolated from various clinical and environmental sources.
CASE PRESENTATION
A 67-year-old man was admitted to the Intensive Care Unit with acute respiratory failure. The patient was diagnosed with bilateral pneumonia, pulmonary sepsis and underwent invasive mechanical ventilation. Examination revealed diminished bilateral vesicular breath sounds, fever, intense yellow tracheal secretions, a respiratory rate of 24/minute, a heart rate of 123/minute, and blood pressure of 75/55 mmHg. Vasoactive treatment was initiated. Investigations revealed elevated lactate and C-reactive protein levels. A chest X-ray showed bilateral infiltration. Parenteral ciprofloxacin and ceftriaxone were administered. Tracheal aspirate culture and blood culture showed bacterial growth of Cupriavidus pauculus. Colistin was added to the treatment. There was a poor clinical response despite repeated blood culture showing negative results. The diagnosis of multiple organ dysfunction syndrome (MODS) caused by C. pauculus was made. The patient died eleven days after admission.
CONCLUSIONS
Clinical improvement cannot always be expected in spite of targeted antibiotic therapy. This pathogen should be considered responsible for infections that usually develop in immunocompromised patients.
PubMed: 29967861
DOI: 10.1515/jccm-2016-0027 -
IDCases 2021Cupriavidus pauculus is a Gram negative rod that is usually isolated in water and has been extraordinarily isolated in patients with vascular access and / or...
Cupriavidus pauculus is a Gram negative rod that is usually isolated in water and has been extraordinarily isolated in patients with vascular access and / or immunosuppression. In this work we present a series of cases of patients with positive blood cultures for cupríavidus pauculus, during a short period of time in our hospital center. The only characteristic related to all cases was the use of parenteral nutrition. It highlights the rareness of the microorganism and the susceptibility identified in the antibiogram, which may be useful for future cases.
PubMed: 33767968
DOI: 10.1016/j.idcr.2021.e01072 -
BMJ Case Reports Jul 2021is a gram-negative bacillus aerobic bacteria widely distributed in nature that can cause, in rare cases, serious infections both in immunocompromised and...
is a gram-negative bacillus aerobic bacteria widely distributed in nature that can cause, in rare cases, serious infections both in immunocompromised and immunocompetent patients. We describe a case of an elderly patient admitted in emergency room with septic shock and diagnosed with a urinary tract infection. During his hospital stay, his clinical and analytical conditions have deteriorated. Blood cultures were positive for only sensitive to minocycline. Despite every effort, due to multiple comorbidities and a nosocomial pneumonia, the patient ends up dying.
Topics: Aged; Anti-Bacterial Agents; Cross Infection; Cupriavidus; Gram-Negative Bacterial Infections; Humans; Immunocompromised Host
PubMed: 34257122
DOI: 10.1136/bcr-2021-243328 -
RSC Advances May 2020Three different methods (a sulfuric acid method, sodium chloride method and vibration method) were used to extract extracellular polymeric substances (EPS) from 1490 (...
Three different methods (a sulfuric acid method, sodium chloride method and vibration method) were used to extract extracellular polymeric substances (EPS) from 1490 ( 1490) in the present study. The sodium chloride method was able to extract the maximum amount of EPS (86.15 ± 1.50 mg g-DW), and could ensure minimum cell lysis by detecting glucose-6-phosphate dehydrogenase activity and using scanning electron microscopy. This method was therefore selected as the optimal extraction method and used in subsequent experiments. On this basis, the tolerance of 1490 and variations in EPS secretion after the addition of different metal ions was investigated. The tolerance levels of 1490 to Cd(ii), Ni(ii), Cu(ii) and Co(ii) were 300 mg L, 400 mg L, 400 mg L and 400 mg L, respectively. Low concentrations of these heavy metal ions could promote bacterial growth, while increased concentrations were found to inhibit it. The results show that metal ions, especially Cd(ii), stimulate the secretion of EPS, with an EPS yield reaching 956.12 ± 10.59 mg g-DW at 100 mg L. Real-time polymerase chain reaction (PCR) analysis showed that the key EPS synthetic genes, , and , were up-regulated. Fourier transform infrared spectroscopy analysis suggested that abundant functional groups in EPS play an important role in heavy metal ion complexation. These results will contribute to our understanding of the tolerance mechanism of microorganisms in the presence of different types and concentrations of metal ions.
PubMed: 35520445
DOI: 10.1039/c9ra10560c -
Cureus Nov 2019While deep brain stimulation (DBS) is now standard therapy for the treatment of Parkinson's disease, essential tremor, and dystonia, infections remain one of the most...
While deep brain stimulation (DBS) is now standard therapy for the treatment of Parkinson's disease, essential tremor, and dystonia, infections remain one of the most common perioperative complications. In this report, we describe a 58-year-old female with a history of medically refractory Parkinson's disease, who underwent magnetic resonance (MR)-guided bilateral subthalamic DBS. While the initial surgery and programming were successful, she returned in follow-up with signs of a generator pocket infection. She was taken to surgery for hardware explantation, and cultures revealed multispecies growth which included the rare Cupriavidus pauculus species. This is the first report of C. pauculus infection in conjunction with a neuromodulation device. We provide a literature review and discussion of C. pauculus, and its implications in the context of DBS surgery.
PubMed: 31886044
DOI: 10.7759/cureus.6104 -
The Journal of Hospital Infection Jul 2018
Topics: Anti-Bacterial Agents; Ciprofloxacin; Colistin; Cupriavidus; Drug Resistance, Bacterial; Drug Therapy, Combination; Female; Gram-Negative Bacterial Infections; Humans; Meropenem; Microbial Sensitivity Tests; Middle Aged; Shock, Septic; Treatment Outcome
PubMed: 29588185
DOI: 10.1016/j.jhin.2018.03.025 -
Journal of Oral Microbiology 2023We aimed to explore saliva microbiome alterations in dental fluorosis population.
BACKGROUND
We aimed to explore saliva microbiome alterations in dental fluorosis population.
METHODS
The prevalence of dental fluorosis was examined in 957 college students. Dean's fluorosis index was used to evaluate the dental fluorosis status. Changes in the composition of the salivary microbiome were assessed in a subset of these patients (100 healthy controls, 100 dental fluorosis patients).
RESULTS
Dental fluorosis affected 47% of the student sample, and incidence was unrelated to gender. Compared with healthy controls, the microbiota of patients with dental fluorosis exhibited increased diversity, with increased abundance of , , , , , and decreased abundance of , , , and . Function analyses showed increases in arginine biosynthesis in patients affected by dental fluorosis, together with reductions in amino sugar and nucleotide sugar metabolism, fructose and mannose metabolism, and starch and sucrose metabolism.
CONCLUSIONS
These results suggest that there are striking differences in salivary microbiome between healthy controls and dental fluorosis patients. Dental fluorosis may contribute to periodontitis and systemic lung diseases. There is a need for cohort studies to determine whether altering the salivary microbiota in dental fluorosis patients can alter the development of oral or systemic diseases.
PubMed: 36844898
DOI: 10.1080/20002297.2023.2180927