-
Cell and Tissue Research Jun 2011Tumor necrosis factor receptor subfamily 9 (TNFRSF9) plays a potentially important general role in immune function. Tnfrsf9 gene expression has previously been...
Tumor necrosis factor receptor subfamily 9 (TNFRSF9) plays a potentially important general role in immune function. Tnfrsf9 gene expression has previously been characterized in late pregnant mouse uterus and placenta. However, little is known about its expression in the uterus during the implantation phase of early pregnancy. We have assessed the levels and localization of Tnfrsf9 expression in the mouse uterus and conceptus during implantation. Relative Tnfrsf9 mRNA levels were significantly higher in implantation than in non-implantation site tissue on days 6.5-8.5 of pregnancy. This increase did not depend on the presence of the conceptus, as mRNA levels were not significantly different between pregnant implantation sites and artificially induced deciduomas. Localization by in situ hybridization revealed a subpopulation of endothelial and uterine natural killer cells expressing Tnfrsf9 in the endometrium during implantation. In the developing conceptus, primary trophoblast giant and ectoplacental cells expressed Tnfrsf9 on days 6.5-8.5, followed by expression in the trophoblast giant cell layers surrounding the conceptus on day 9.5 of pregnancy. Two main splice forms of Tnfrsf9 mRNA exist and encode proteins with distinct biological functions; both mRNA splice forms were present in uterine and conceptus tissues as determined by reverse transcription with the polymerase chain reaction. Thus, both membrane and soluble forms of Tnfrsf9 are expressed in specific cell types of the uterus and conceptus during the progression of implantation in mice and possibly have an important function in this process.
Topics: Animals; Decidua; Embryo Implantation; Female; Gene Expression; Male; Mice; Mice, Knockout; Pregnancy; RNA, Messenger; Tumor Necrosis Factor Receptor Superfamily, Member 9; Uterus
PubMed: 21560035
DOI: 10.1007/s00441-011-1171-0 -
Endocrine Journal Aug 1996The role of mesencephalic raphe nuclei in the induction of pseudopregnancy was investigated in female rats. The dorsal or median raphe nucleus lesions (DRL or MRL,...
The role of mesencephalic raphe nuclei in the induction of pseudopregnancy was investigated in female rats. The dorsal or median raphe nucleus lesions (DRL or MRL, respectively) were made by means of a radiofrequency lesion generator. Two or 3 weeks after the operation, in order to induce pseudopregnancy, the vagina was stimulated electrically on the day of proestrus or 1 mg/kg b.w. reserpine was injected on the day of diestrus I. Traumatization by passing thread to one uterine horn was performed to induce deciduoma 5 days after vaginal stimulation or 3 days after reserpine injection. As the results, decidual response was seen in most control and sham females in both vaginal stimulation and reserpine-treated groups. In contrast, incidences of deciduoma in DRL females with vaginal stimulation or reserpine-injection were significantly lower than those in control and sham groups. In the MRL females with either vaginal stimulation or reserpine-treatment, incidences of deciduoma were comparable to those of the control and sham operated groups. These results suggest that the dorsal raphe nucleus plays an important role in pseudopregnancy-inducing mechanisms in female rats.
Topics: Animals; Decidua; Electric Stimulation; Female; Proestrus; Pseudopregnancy; Raphe Nuclei; Rats; Rats, Wistar; Reserpine; Vagina
PubMed: 8930524
DOI: 10.1507/endocrj.43.369 -
The Anatomical Record Mar 1996A decidual cell reaction can be induced in rodent endometrium by an intrauterine injection of oil. The epithelial lining is thought to be instrumental to transduce...
BACKGROUND
A decidual cell reaction can be induced in rodent endometrium by an intrauterine injection of oil. The epithelial lining is thought to be instrumental to transduce intralumenal stimuli for decidualization. One of the consequences of oil injection is the death of uterine epithelial cells. No information is available on the effect that sustained contact with oil has on the epithelium.
METHODS
A decidual cell reaction was induced in 4-day pseudopregnant mice by injection of 30 microliters of arachis oil into the uterine lumen. Samples from the uteri were collected 24, 48, and 72 h after the injection and prepared for transmission electron microscopy.
RESULTS
Twenty-four hours after the oil injection, some of the initial modifications of epithelial cell surfaces were very similar to those induced by the contact with the blastocyst during normal pregnancy. Uterine epithelial cells internalized injected oil and many cells were seen in various stages of degeneration. At 48 h, many epithelial cells were detached from the basal lamina. At 72 h, the uterine lining was re-established by flattened cells.
CONCLUSIONS
The contact of oil with the uterine epithelium of pseudo pregnant mice induces epithelial cell death in the antimesometrial region of the uterine crypt. There is, however, replacement of epithelial lining by epithelial cells, which probably migrate from the mesometrial region of the crypt. The prolonged presence of oil within the uterine lumen seems to induce cycles of epithelial cell death and replacement.
Topics: Animals; Cell Death; Cell Division; Decidua; Endometrium; Female; Mice; Microscopy, Electron; Peanut Oil; Plant Oils; Pregnancy; Time Factors
PubMed: 8742697
DOI: 10.1002/(SICI)1097-0185(199603)244:3<316::AID-AR4>3.0.CO;2-W -
Developmental Biology Jun 2006The uterus undergoes a series of dramatic changes in response to an implanting conceptus that, in some mammalian species, includes differentiation of the endometrial...
The uterus undergoes a series of dramatic changes in response to an implanting conceptus that, in some mammalian species, includes differentiation of the endometrial stroma into decidual tissue. This process, called decidualization, can be induced artificially in rodents indicating that the conceptus may not be essential for a proper maternal response in early pregnancy. In order to test this hypothesis, we determined if and how the conceptus affects uterine gene expression. We identified 5 genes (Angpt1, Angpt2, Dtprp, G1p2 and Prlpa) whose steady-state levels in the uterus undergoing decidualization depends on the presence of a conceptus. In situ hybridization revealed region-specific effects which suggested that various components of the conceptus and more than one signal from the conceptus are likely responsible for altering decidual cell function. Using cell culture models we found that trophoblast giant cells secrete a type I interferon-like molecule which can induce G1p2 expression in endometrial stromal cells. Finally, decidual Prlpa expression was reduced in the uterus adjacent to Hand1- and Ets2-deficient embryos, suggesting that normal trophoblast giant cells in the placenta are required for the conceptus-dependent effects on Prlpa expression in the mesometrial decidua. Overall, these results provide support for the hypothesis that molecular signals from the mouse conceptus have local effects on uterine gene expression during decidualization.
Topics: Angiopoietin-Like Protein 2; Angiopoietin-like Proteins; Angiopoietins; Animals; Basic Helix-Loop-Helix Transcription Factors; Blood Proteins; Cells, Cultured; Cytokines; Decidua; Deciduoma; Embryo, Mammalian; Endometrium; Female; Gene Expression Profiling; Gene Expression Regulation, Developmental; Humans; In Situ Hybridization; Intercellular Signaling Peptides and Proteins; Interferons; Male; Mice; Oligonucleotide Array Sequence Analysis; Paracrine Communication; Pregnancy; Pregnancy Proteins; Prolactin; Proto-Oncogene Protein c-ets-2; Ubiquitins
PubMed: 16616120
DOI: 10.1016/j.ydbio.2006.03.006 -
Veterinary Pathology Jul 2007The ovaries and uterus were collected after ovariohysterectomy from a 16-month-old Labrador bitch in diestrus that never mated. Discrete swellings were found in the...
The ovaries and uterus were collected after ovariohysterectomy from a 16-month-old Labrador bitch in diestrus that never mated. Discrete swellings were found in the uterine horns, with the macroscopic appearance of normal early pregnancy. At histologic examination, the endometrium, devoid of any conceptus and chorion, showed a marked proliferation, on the basis of which a diagnosis of deciduoma was made. A remarkable population of stromal eosinophilic granular lymphocytes was present, especially in the axis of the endometrial folds. Periodic acid-Schiff and Dolichos biflorus-lectin histochemical reaction and a panel of 10 immunohistochemical markers were used to characterize eosinophilic granular cells. Our findings allowed us to compare these granular cells with the granulated decidual cells, whose presence was until now described only in primates, rodents, or a few other epitheliochorial species. On the basis of our results, the importance of eosinophilic granular cells in a decidualization process is hypothesized to occur also in the bitch.
Topics: Animals; Deciduoma; Dog Diseases; Dogs; Female; Immunohistochemistry; Lymphocytes
PubMed: 17606515
DOI: 10.1354/vp.44-4-521 -
Endocrinologia Japonica Feb 1975When rats ovariectomized at 3 days of age and given a single injection of 1.25 mg testosterone propionate on the next day became 60 days old, they were given 3 daily...
When rats ovariectomized at 3 days of age and given a single injection of 1.25 mg testosterone propionate on the next day became 60 days old, they were given 3 daily injections of 0.2 mug estradiol-17 beta followed by 7 daily combined injections of 2 mg progesterone and 0.2 mug estradiol. Incidence of deciduomata in reaction to uterine trauma applied on the 4th day of the progesterone-estradiol injections was almost as high as that in neonatally ovariectomized, non-androgenized rats, but the response was significantly smaller in size in androgenized rats than in non-androgenized animals. If females similarly operated on were given injections of 0.1 mug estradiol for 30 days prior to 7 daily injections of progesterone-estradiol, deciduoma formation in androgenized rats was markedly reduced in both incidence and size of the response. In non-androgenized group, deciduoma formation was not significantly affected by chronic administration of estradiol.Accordingly, it is likely that, although androgen injected during neonatal life is responsible for the reduction of uterine responsiveness in androgen-sterilized rats (Takewaki and Ohta, 1974) continued exposure of the uterus to estrogen may play a co-operative role in the event.
Topics: Animals; Castration; Decidua; Estradiol; Female; Progesterone; Rats; Testosterone; Uterine Diseases; Vagina
PubMed: 1126325
DOI: 10.1507/endocrj1954.22.79 -
The Journal of Veterinary Medical... Mar 1997Bitches were examined to see whether canine deciduoma could be induced at some reproductive stages with the different conditions of corpora lutea by inserting a silk...
Bitches were examined to see whether canine deciduoma could be induced at some reproductive stages with the different conditions of corpora lutea by inserting a silk suture into the uterine lumen. The bitches stimulated in the early and middle stages of diestrus or in unilateral pregnancy corresponding to these diestrous stages formed deciduoma at a high induction rate, however, no difference in the strength of decidual reaction between the pregnant and diestrous stages was recognized. On the other hand, no reaction could be seen in bitches in late diestrus, the late stage of unilateral pregnancy or the post partum repair phase in which stromal decidual cells similar to those of the rodentia can be seen. In already implanted uteri, however, no deciduoma was formed in the interplacental areas. Even though the corpora lutea were functional, new additional stimulations were not accepted at the interplacental area in which the uterine horn had already been influenced by fertilized ova. From these results, it was suggested that in the dog as well as the rodentia, the endometrium has to be under the influence of functional corpora lutea in order to form deciduoma.
Topics: Animals; Cell Division; Corpus Luteum; Decidua; Dogs; Estrus; Female; Ovary; Pregnancy; Reproduction; Uterus
PubMed: 9101477
DOI: 10.1292/jvms.59.185 -
The Journal of Veterinary Medical... May 1998Histological variations of canine deciduoma which was induced in the non pregnant horn at several stages of unilateral pregnancy were examined. In the first half of the...
Histological variations of canine deciduoma which was induced in the non pregnant horn at several stages of unilateral pregnancy were examined. In the first half of the unilateral pregnancy, deciduoma was characterized by the cystic glandular hyperplasia corresponding to each of the stages in normal early placentation. In the second half, deciduoma could not be induced and few histological reactions were recognized. The endometrium looked normal for late diestrus with no growth of the uterine glands. These differences might reflect the latent strength of the uterine glands to proliferate and dilate in the stimulated periods.
Topics: Animals; Cell Division; Decidua; Diestrus; Dogs; Endometrium; Female; Gestational Age; Hyperplasia; Hysterectomy; Placenta; Pregnancy; Pregnancy, Animal; Time Factors
PubMed: 9637298
DOI: 10.1292/jvms.60.623 -
British Journal of Pharmacology and... Jun 1963The effects of chlorpromazine and perphenazine were studied with both immature and mature female mice. At a dose of 5 mg/kg daily, each drug delayed the onset of sexual...
The effects of chlorpromazine and perphenazine were studied with both immature and mature female mice. At a dose of 5 mg/kg daily, each drug delayed the onset of sexual maturity in immature animals. In mature mice, a daily dose of 10 mg/kg inhibited vaginal oestrus and reduced the weights of the ovaries, vagina and uterus. Each of the two drugs inhibited ovarian hypertrophy following unilateral ovariectomy, but only slightly diminished the responses to exogenous oestrogen and to exogenous gonadotrophin. In one experiment, pseudopregnancy, as shown by a positive deciduoma-test, was provoked by perphenazine, but this experiment could not be repeated. Chlorpromazine failed to evoke a positive deciduoma response. At a dose of 10 mg/kg, neither drug caused a fall in levels of adrenal ascorbic acid. In the rat, treatment for 1 week with 10 mg/kg daily of either drug led to a rise in pituitary gonadotrophic activity. It was concluded that both drugs inhibited the release of gonadotrophins by the pituitary gland, independently of any action in releasing corticotrophin.
Topics: Animals; Antipsychotic Agents; Chlorpromazine; Female; Genitalia, Female; Humans; Mice; Ovary; Perphenazine; Phenothiazines; Rats; Uterus; Vagina
PubMed: 14028829
DOI: 10.1111/j.1476-5381.1963.tb01486.x -
British Medical Journal Dec 1896
PubMed: 20756680
DOI: 10.1136/bmj.2.1878.1819