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Genome Announcements Jul 2014Dermabacter hominis is a common colonizer of the healthy human skin and is rarely detected as an opportunistic human pathogen. The genome sequence of the...
Dermabacter hominis is a common colonizer of the healthy human skin and is rarely detected as an opportunistic human pathogen. The genome sequence of the multidrug-resistant D. hominis strain 1368, isolated from blood cultures of a pyelonephritis patient, provides insights into the repertoire of antibiotic resistance genes.
PubMed: 25059872
DOI: 10.1128/genomeA.00728-14 -
Journal of Clinical Microbiology Jun 2001Dermabacter hominis is a gram-positive, catalase-positive, glucose-fermenting rod, which, as it grows forms small greyish-white colonies with a characteristic pungent...
Dermabacter hominis is a gram-positive, catalase-positive, glucose-fermenting rod, which, as it grows forms small greyish-white colonies with a characteristic pungent odor. Previously known as coryneform Centers for Disease Control and Prevention groups 3 and 5, it was catalogued as D. hominis in 1994. Various strains isolated in blood cultures, abscesses, or wounds in the 1970s were retrospectively characterized in referral centers as D. hominis. In this report we describe two patients with severe underlying pathology who developed bacteremias by D. hominis within the context of their clinical pictures.
Topics: Actinomycetales; Actinomycetales Infections; Adult; Aged; Bacteremia; Blood; Culture Media; Female; Humans; Male
PubMed: 11376092
DOI: 10.1128/JCM.39.6.2356-2357.2001 -
Antimicrobial Agents and Chemotherapy Dec 1996The susceptibility patterns of 480 isolates representing six recently defined species of coryneform bacteria (Corynebacterium amycolatum [n = 101], Corynebacterium auris...
The susceptibility patterns of 480 isolates representing six recently defined species of coryneform bacteria (Corynebacterium amycolatum [n = 101], Corynebacterium auris [n = 48], Corynebacterium glucuronolyticum [n = 86], Brevibacterium casei [n = 50], Dermabacter hominis [n = 49], and Turicella otitidis [n = 146]) to 17 antimicrobial agents were determined by an agar dilution method. Most significantly, for C. amycolatum strains the MICs at which 90% of isolates are inhibited were > or = 32 micrograms/ml for nearly all agents. However, all 480 strains examined were susceptible to glycopeptide antibiotics.
Topics: Actinomycetales; Anti-Bacterial Agents; Drug Resistance, Microbial; Glycopeptides; Microbial Sensitivity Tests
PubMed: 9124857
DOI: 10.1128/AAC.40.12.2874 -
BMC Microbiology Jan 2024Reliable species identification of cultured isolates is essential in clinical bacteriology. We established a new study algorithm named NOVA - Novel Organism Verification...
Novel Organism Verification and Analysis (NOVA) study: identification of 35 clinical isolates representing potentially novel bacterial taxa using a pipeline based on whole genome sequencing.
BACKGROUND
Reliable species identification of cultured isolates is essential in clinical bacteriology. We established a new study algorithm named NOVA - Novel Organism Verification and Analysis to systematically analyze bacterial isolates that cannot be characterized by conventional identification procedures MALDI-TOF MS and partial 16 S rRNA gene sequencing using Whole Genome Sequencing (WGS).
RESULTS
We identified a total of 35 bacterial strains that represent potentially novel species. Corynebacterium sp. (n = 6) and Schaalia sp. (n = 5) were the predominant genera. Two strains each were identified within the genera Anaerococcus, Clostridium, Desulfovibrio, and Peptoniphilus, and one new species was detected within Citrobacter, Dermabacter, Helcococcus, Lancefieldella, Neisseria, Ochrobactrum (Brucella), Paenibacillus, Pantoea, Porphyromonas, Pseudoclavibacter, Pseudomonas, Psychrobacter, Pusillimonas, Rothia, Sneathia, and Tessaracoccus. Twenty-seven of 35 strains were isolated from deep tissue specimens or blood cultures. Seven out of 35 isolated strains identified were clinically relevant. In addition, 26 bacterial strains that could only be identified at the species level using WGS analysis, were mainly organisms that have been identified/classified very recently.
CONCLUSION
Our new algorithm proved to be a powerful tool for detection and identification of novel bacterial organisms. Publicly available clinical and genomic data may help to better understand their clinical and ecological role. Our identification of 35 novel strains, 7 of which appear to be clinically relevant, shows the wide range of undescribed pathogens yet to define.
Topics: Bacteria; Whole Genome Sequencing; Corynebacterium; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; RNA, Ribosomal, 16S; Bacterial Typing Techniques
PubMed: 38178003
DOI: 10.1186/s12866-023-03163-7 -
International Journal of Infectious... Jun 2015Breast implant infections are usually caused by Staphylococcus aureus and coagulase-negative staphylococci. Gram-negative bacilli are rarely reported to be involved in...
BACKGROUND
Breast implant infections are usually caused by Staphylococcus aureus and coagulase-negative staphylococci. Gram-negative bacilli are rarely reported to be involved in breast implant infections.
METHODS
Thirty-seven cases of microbiologically confirmed breast implant infection managed from January 2008 to June 2012 in the study centre were reviewed, including 10 cases from the study centre itself and 27 cases from private clinics in the region.
RESULTS
The prevalence of breast implant infection in the study centre was 0.74% of breast implantation, i.e., 3.23% in breast reconstruction for breast cancer and 0.27% in aesthetic breast augmentation (p=0.0002). Of the 37 cases, 30% had undergone radiotherapy and 11% had undergone a lymph node dissection. S. aureus was identified in 18 cases, Gram-negative bacilli in 10 cases, coagulase-negative staphylococci in eight cases, anaerobic bacteria in eight cases, and streptococci in three cases. Pseudomonas aeruginosa was the second most commonly identified pathogen. Staphylococcus epidermidis was the most frequent coagulase-negative Staphylococcus species. In addition to Propionibacterium acnes and Actinomyces neuii, other facultative and strict anaerobic bacteria have not been reported before, e.g., Bacteroides thetaiotaomicron, Corynebacterium simulans, Dermabacter hominis, Finegoldia magna, and Peptoniphilus harei. Seventy-percent of cases were treated by immediate implant removal. All cases treated only with antibiotics were treated with surgery at the second visit.
CONCLUSIONS
The microbiological epidemiology was noted by an increasing the proportion of Gram-negative bacteria and anaerobic bacteria detected with the advent of MALDI-TOF MS and molecular identification for diagnosis.
Topics: Adult; Aged; Bacteria; Bacterial Infections; Breast Diseases; Breast Implants; Female; France; Gram-Negative Bacteria; Humans; Mammaplasty; Middle Aged; Postoperative Complications; Pseudomonas aeruginosa; Referral and Consultation; Staphylococcus; Staphylococcus aureus; Young Adult
PubMed: 25910855
DOI: 10.1016/j.ijid.2015.04.010 -
Revista Do Instituto de Medicina... 2020Myiasis represents a group of neglected tropical diseases caused by the infestation of vertebrate tissues by dipterous larvae. We herein report an imported case of...
Myiasis represents a group of neglected tropical diseases caused by the infestation of vertebrate tissues by dipterous larvae. We herein report an imported case of foruncular myasis caused by Dermatobia hominis in Mexico City. The species was confirmed by DNA sequencing and phylogenetic reconstruction analysis.
Topics: Actinobacteria; Adult; Animals; Diptera; Humans; Larva; Mexico; Myiasis; Phylogeny; Sequence Analysis, DNA; Skin Diseases, Parasitic; Travel
PubMed: 32667394
DOI: 10.1590/s1678-9946202062047