-
Journal of Dairy Science Jan 2016The aim of this study was to evaluate the effect of stage of maturity at harvest on extent of starch, neutral detergent fiber (NDF), and protein digestion, and rumen...
The aim of this study was to evaluate the effect of stage of maturity at harvest on extent of starch, neutral detergent fiber (NDF), and protein digestion, and rumen fermentation in dry cows fed whole-plant corn silage from different hybrids. Four nonlactating Holstein cows cannulated at the rumen and proximal duodenum were fed 4 corn silages differing in hybrid (flint vs. flint-dent) and maturity stage (early vs. late) in a 4 × 4 Latin square design. From early to late maturity, starch content increased (from 234.5 to 348.5 g/kg), whereas total-tract (99.7 to 94.5%) and ruminal starch digestibility (91.3 to 86.5%) decreased significantly. The decrease in ruminal starch digestibility with increasing maturity was similar between hybrids. No effects were found of maturity, hybrid, or maturity × hybrid interaction on total-tract NDF digestibility, ruminal NDF digestibility, true digestibility of N and organic matter in the rumen, or microbial synthesis. Harvesting at later maturity led to increased ruminal ammonia, total volatile fatty acid concentrations, and acetate/propionate ratio but not pH. This study concludes that delaying date of harvest modifies the proportions of digestible starch and NDF supplied to cattle. Adjusting date of corn harvest to modulate amount of rumen-digested starch could be used as a strategy to control nutrient delivery to ruminants.
Topics: Animals; Cattle; Dietary Fiber; Digestion; Fatty Acids, Volatile; Female; Fermentation; Hydrogen-Ion Concentration; Intestinal Mucosa; Rumen; Silage; Starch; Zea mays
PubMed: 26585483
DOI: 10.3168/jds.2015-9466 -
Molecules (Basel, Switzerland) Jun 2021The present investigation aimed to provide novel information on the chemical composition and in vitro bioaccessibility of bioactive compounds from raw citrus pomaces...
The present investigation aimed to provide novel information on the chemical composition and in vitro bioaccessibility of bioactive compounds from raw citrus pomaces (mandarin varieties Clemenule and Ortanique and orange varieties Navel and Valencia). The effects of the baking process on their bioaccessibility was also assessed. Samples of pomaces and biscuits containing them as an ingredient were digested, mimicking the human enzymatic oral gastrointestinal digestion process, and the composition of the digests were analyzed. UHPLC-MS/MS results of the citrus pomaces flavonoid composition showed nobiletin, hesperidin/neohesperidin, tangeretin, heptamethoxyflavone, tetramethylscutellarein, and naringin/narirutin. The analysis of the digests indicated the bioaccessibility of compounds possessing antioxidant [6.6-11.0 mg GAE/g digest, 65.5-97.1 µmol Trolox Equivalents (TE)/g digest, and 135.5-214.8 µmol TE/g digest for total phenol content (TPC), ABTS, and ORAC-FL methods, respectively; significant reduction ( < 0.05) in Reactive Oxygen Species (ROS) formation under tert-butyl hydroperoxide (1 mM)-induced conditions in IEC-6 and CCD-18Co cells when pre-treated with concentrations 5-25 µg/mL of the digests], anti-inflammatory [significant reduction ( < 0.05) in nitric oxide (NO) production in lipopolysaccharide (LPS)-induced RAW264.7 macrophages], and antidiabetic (IC 3.97-11.42 mg/mL and 58.04-105.68 mg/mL for α-glucosidase and α-amylase inhibition capacities) properties in the citrus pomaces under study. In addition, orange pomace biscuits with the nutrition claims "no-added sugars" and "source of fiber", as well as those with good sensory quality (6.9-6.7, scale 1-9) and potential health promoting properties, were obtained. In conclusion, the results supported the feasibility of citrus pomace as a natural sustainable source of health-promoting compounds such as flavonoids. Unfractionated orange pomace may be employed as a functional food ingredient for reducing the risk of pathophysiological processes linked to oxidative stress, inflammation, and carbohydrate metabolism, such as diabetes, among others.
Topics: Animals; Anti-Inflammatory Agents; Antioxidants; Cell Line; Chromatography, High Pressure Liquid; Citrus; Citrus sinensis; Digestion; Flavonoids; Fruit; Gastrointestinal Tract; Humans; Mice; Oxidative Stress; Plant Extracts; RAW 264.7 Cells; Tandem Mass Spectrometry
PubMed: 34201056
DOI: 10.3390/molecules26123480 -
Food Research International (Ottawa,... Nov 2019In vitro digestion models are a valid methodology to study nutrient hydrolysis by simulating standard physiological gastrointestinal conditions. However, there are...
In vitro digestion models are a valid methodology to study nutrient hydrolysis by simulating standard physiological gastrointestinal conditions. However, there are pathologies in which some conditions are affected, which should be considered in the design of an in vitro digestion study. Our work aims at elucidating the role of different gastrointestinal conditions on lipolysis. In the context of exocrine pancreatic insufficiency, gastric pH, intestinal pH, bile salts composition, bile salts concentration, fat concentration in the digestion medium and volumetric ratio digestion fluid/food were the selected study parameters. The pH-stat method was applied to assess lipolysis extent and kinetics. Descriptive results were summarised in digestibility curves and beta regression models were used to explain the effect (odds ratio, OR) of the studied conditions on lipolysis. Additionally, distribution of emulsion droplets was measured and optical microscopy images of fat globules were taken in a selection of experimental conditions. Results showed that intestinal pH was the variable with the highest effect on lipolysis (OR 22.86, p < 0.001), followed by fat concentration in the digestion medium (OR 6.76, p < 0.001) and bile salts concentration (OR 1.56, p < 0.001). Overall, lipolysis was significantly associated with particle size (OR - 6.98, p < 0.001). We conclude that the assessment of lipolysis by means of in vitro digestion models is sensitive to the simulated gastrointestinal conditions, which should be adapted to the real physiological conditions occurring in altered health conditions.
Topics: Animals; Bile Acids and Salts; Cattle; Digestion; Gastric Juice; Humans; Hydrogen-Ion Concentration; Intestines; Lipolysis; Models, Biological; Stomach; Swine
PubMed: 31554063
DOI: 10.1016/j.foodres.2019.108511 -
Poultry Science Mar 2019A total of 90 male Ross 308 broiler chicks were used in a digestibility and performance bioassay to explore the effect of reduction in dietary protein and digestible...
A mono-component microbial protease improves performance, net energy, and digestibility of amino acids and starch, and upregulates jejunal expression of genes responsible for peptide transport in broilers fed corn/wheat-based diets supplemented with xylanase and phytase.
A total of 90 male Ross 308 broiler chicks were used in a digestibility and performance bioassay to explore the effect of reduction in dietary protein and digestible amino acids and inclusion of an exogenous mono-component protease on amino acid digestibility, net energy, jejunal gene expression, and bird performance. Four dietary treatments were created by the supplementation, or not, of 2 control diets with a mono-component exogenous protease. The control diets were corn/wheat/soybean meal-based and were formulated to be either nutritionally adequate or reduced in protein and amino acids (around 3%). The 2 control diets were supplemented with xylanase and phytase (2000 FYT). Treatments were therefore arranged as a 2 × 2 factorial design. The reduction in diet nutrient density had no significant effect on various experimental outcomes (including bird performance, amino acid digestibility, and net energy [NE]) that were measured with the exception of a reduction in the expression of aminopeptidase N and glucose transporter 2. However, the addition of exogenous protease resulted in an increase in weight gain and a reduction in feed conversion ratio (around 4%; P < 0.05) and an increase in the digestibility of several amino acids (P < 0.05) and starch (P = 0.06). Protease addition also resulted in an increase in both apparent metabolizable energy (AME) (+73 kcal/kg; P < 0.05) and NE (+107 kcal/kg; P < 0.05). The addition of exogenous protease to the diet also increased the jejunal expression of genes responsible for peptide transport (PepT2; P < 0.01) and starch digestion (sucrase isomaltase; P = 0.06). These results confirm the efficacy of exogenous protease in broiler diets that contain both xylanase and phytase and suggest substantial beneficial effects that extend beyond protein and amino acid nutrition. The effect of exogenous protease on energy partitioning, starch digestibility and the efficiency of nitrogen cycling is an area for further study.
Topics: 6-Phytase; Amino Acids; Animal Feed; Animal Nutritional Physiological Phenomena; Animals; Bacillus licheniformis; Chickens; Diet; Dietary Proteins; Digestion; Endo-1,4-beta Xylanases; Jejunum; Male; Serine Proteases; Starch; Triticum; Zea mays
PubMed: 30289499
DOI: 10.3382/ps/pey456 -
PloS One 2017In vivo assays cannot always be conducted because of ethical reasons, technical constraints or costs, but a better understanding of the digestive process, especially in...
BACKGROUND
In vivo assays cannot always be conducted because of ethical reasons, technical constraints or costs, but a better understanding of the digestive process, especially in infants, could be of great help in preventing food-related pathologies and in developing new formulas with health benefits. In this context, in vitro dynamic systems to simulate human digestion and, in particular, infant digestion could become increasingly valuable.
OBJECTIVE
To simulate the digestive process through the use of a dynamic model of the infant gastroenteric apparatus to study the digestibility of starch-based infant foods.
DESIGN
Using M.I.D.A (Model of an Infant Digestive Apparatus), the oral, gastric and intestinal digestibility of two starch-based products were measured: 1) rice starch mixed with distilled water and treated using two different sterilization methods (the classical method with a holding temperature of 121°C for 37 min and the HTST method with a holding temperature of 137°C for 70 sec) and 2) a rice cream with (premium product) or without (basic product) an aliquot of rice flour fermented by Lactobacillus paracasei CBA L74. After the digestion the foods were analyzed for the starch concentration, the amount of D-glucose released and the percentage of hydrolyzed starch.
RESULTS
An in vitro dynamic system, which was referred to as M.I.D.A., was obtained. Using this system, the starch digestion occurred only during the oral and intestinal phase, as expected. The D-glucose released during the intestinal phase was different between the classical and HTST methods (0.795 grams for the HTST versus 0.512 for the classical product). The same analysis was performed for the basic and premium products. In this case, the premium product had a significant difference in terms of the starch hydrolysis percentage during the entire process.
CONCLUSIONS
The M.I.D.A. system was able to digest simple starches and a more complex food in the correct compartments. In this study, better digestibility of the premium product was revealed.
Topics: Body Fluids; Digestion; Electrolytes; Fermentation; Gastrointestinal Tract; Glucose; Humans; Hydrolysis; Infant; Models, Biological; Oryza; Starch; Sterilization
PubMed: 29261742
DOI: 10.1371/journal.pone.0189807 -
Journal of Dairy Science Sep 2010New fractionation and fermentation technologies in the ethanol industry have resulted in the production of different forms of distillers grains (DG). Such products are...
New fractionation and fermentation technologies in the ethanol industry have resulted in the production of different forms of distillers grains (DG). Such products are reduced-fat, high-protein, and "modified" wet feeds. Characterization of protein fractions of these co-products and other commonly used feedstuffs is important for the formulation of dairy cattle diets. In situ and in vitro techniques were conducted to compare crude protein (CP) availability in 4 DG products with commonly used soybean proteins. Soybean protein products included solvent-extracted soybean meal (SBM; 44% CP), expeller soybean meal (ESBM), and extruded soybeans (ES). The DG products were conventional distillers dried grains with solubles, reduced-fat distillers dried grains with solubles (RFDGS), high-protein distillers dried grains, and modified wet distillers grains with solubles (MWDGS). Nylon bags containing 5 g of each feed were incubated in the rumen of 3 cannulated lactating cows for 2, 4, 8, 16, 24, and 48 h. The rapidly degradable CP fraction varied from 8.1 to 37.2% for SBM and MWDGS, respectively. The slowly degradable CP fraction was greatest for SBM, ES, and high-protein distillers dried grains (88.0%+/-3.7), followed by ESBM, distillers dried grains with solubles, and RFDGS (76.8+/-4.1%). The MWDGS had the lowest slowly degradable CP fraction (61.1%). The rate of degradation of the slowly degradable CP fraction ranged from 11.8 for SBM to 2.7%/h for RFDGS. Rumen-undegradable protein varied widely (32.3 to 60.4%), with RFDGS having the greatest and SBM the lowest concentrations. Intestinal digestibility of rumen-undegradable protein (IDP) was estimated by pepsin-pancreatin digestion of ruminally preincubated (16 h) samples. The IDP was greatest for SBM, ESBM, and ES (97.7%+/-0.75), whereas IDP of DG products was 92.4%+/-0.87. Similarly, total digestible protein was greatest (99.0%) for soybean products, whereas DG products had a total digestible protein of 96.0%. Intestinal digestibility of most AA in DG products exceeded 92% and was slightly lower than for soybean products, except for Lys, where the digestibility was 84.6% for DG compared with 97.3% for soybean products. Absorbable Lys was lower for DG (7.0 g/kg of CP) compared with ESBM and ES (average of 23.8 g/kg of CP). Dried DG, ESBM, and ES provided more absorbable AA compared with SBM and MWDGS. These results suggest that the AA availability from DG products is comparable with that from soybean products.
Topics: Amino Acids; Animal Feed; Animals; Cattle; Dietary Proteins; Digestion; Female; Intestinal Mucosa; Intestines; Rumen; Soybean Proteins; Glycine max; Zea mays
PubMed: 20723689
DOI: 10.3168/jds.2009-2883 -
The Journal of Experimental Biology Apr 2006Using red kangaroos Macropus rufus Desmarest, a large (>20 kg) marsupial herbivore, we compared the digestive capabilities of juveniles with those of mature,...
Using red kangaroos Macropus rufus Desmarest, a large (>20 kg) marsupial herbivore, we compared the digestive capabilities of juveniles with those of mature, non-lactating females on high-quality forage (chopped lucerne Medicago sativa hay) of 43+/-1% neutral-detergent fibre (NDF) and poorer quality, high-fibre forage (chopped oaten Avena sativa hay) of 64+/-1% NDF. On chopped lucerne apparent dry matter (DM) digestibilities by young-at-foot (YAF) red kangaroos (an age that would normally be taking some milk from their mother), weaned juveniles and mature females were similar (55-59%). On chopped oaten hay apparent DM digestibility was lower in the YAF (35.9+/-2.3%) followed by weaned (43.4+/-2.8%) and mature females (44.6+/-1%). The digestion of NDF and its components (mainly cellulose and hemicellulose) was lowest among the YAF followed by weaned and then mature females. The YAF and weaned kangaroos could not sustain growth on the poor-quality diet, and appeared to be at or near maximal gut fill on both forages; the values being 114-122 g DM for YAF and 151-159 g DM for weaned kangaroos. Mean retention times (MRT) of particle and solute markers were significantly longer for the YAF and weaned kangaroos on oaten hay than on lucerne hay, and DM intake (g d(-1)) was approximately 50% lower on the oaten hay. In contrast, solute and particle MRTs in the mature females were not significantly affected by diet; they maintained DM intakes by increasing DM gut fill from 264+/-24 g on chopped lucerne to 427+/-26 g DM on chopped oaten hay. Clearly, the mature female kangaroos did not maximise gut fill on the high-quality forage, presumably as a consequence of their proportionally lower energy requirements compared with still-growing juveniles. Overall, we have provided the first mechanistic link between the physiological constraints faced by juvenile red kangaroos in relation to their drought-related mortalities, rainfall and forage quality.
Topics: Aging; Animal Feed; Animals; Body Size; Dietary Fiber; Digestion; Female; Gastrointestinal Contents; Gastrointestinal Motility; Macropodidae; Male
PubMed: 16574810
DOI: 10.1242/jeb.02137 -
Food Research International (Ottawa,... Jul 2020Whey protein isolate (WPI) interactions with (-)-epigallocatechin gallate (EGCG) and caffeic acid (CA) at pH 3.5 and 7.0 were investigated concerning complex formation...
Whey protein isolate (WPI) interactions with (-)-epigallocatechin gallate (EGCG) and caffeic acid (CA) at pH 3.5 and 7.0 were investigated concerning complex formation and antioxidant capacity, before and after simulated digestion. Complex formation was evidenced by protein structural changes when WPI was associated with CA or EGCG. Reducing capacity and FRAP values increased as the phenolic compound concentration increased while ORAC values remained unchanged. In general, compared to the isolated compounds, complexation suppressed the antioxidant capacity possibly due to hydrophobic interaction and H-bonding between these compounds. Protein:phenolic complexation in 1:0.5 M ratio did not affect the digestibility compared to WPI (83%), except for WPI:CA at pH 7.0 (73%). The hydrophilicity profile of the digested samples suggested that pH of complexation and type of phenolic affected the protein cleavage pattern. Furthermore, the phenolic compounds were more stable when associated with the protein since they were protected from the simulated gastrointestinal environment.
Topics: Antioxidants; Caffeic Acids; Catechin; Digestion; Gastrointestinal Tract; Hydrogen Bonding; Hydrophobic and Hydrophilic Interactions; In Vitro Techniques; Phenols; Whey Proteins
PubMed: 32466919
DOI: 10.1016/j.foodres.2020.109104 -
Current Biology : CB Jul 2019Direct indicators of diet and predator-prey relationships are exceedingly rare in the fossil record [1, 2]. However, it is through such traces that we can best...
Direct indicators of diet and predator-prey relationships are exceedingly rare in the fossil record [1, 2]. However, it is through such traces that we can best understand trophic interactions in ancient ecosystems [3], confirm dietary inferences derived from skeletal morphologies [4], and clarify behavioral and ecological interpretations [5]. Here, we identify a previously unrecognized lizard species in the abdomen of a specimen of Microraptor zhaoianus, a small, volant dromaeosaurid (Paraves) with asymmetrical flight feathers on both its forelimbs and hindlimbs from the Early Cretaceous Jehol Biota [6-8]. The lizard is largely complete and articulated, confirming the current perception of Microraptor as an agile opportunistic predator that, like extant reptiles, including raptorial birds, ingested small prey whole and head first [9]. The lizard can be readily distinguished from previously recognized Early Cretaceous species based on its unusual widely spaced and brachydont dentition. Phylogenetic analysis suggests Indrasaurus wangi gen. et sp. nov. is a basal scleroglossan closely related to the slightly older Liushusaurus [10]. Comparison of ingested remains preserved across Paraves suggests that dromaeosaurids retained the plesiomorphic condition in which ingested prey were fully digested, rather than egested, as has been demonstrated was the case in the probable troodontid Anchiornis [11]. This supports a closer relationship between Aves and Anchiornis [12, 13] and suggests that flight did not precipitate the evolution of pellet egestion in Paraves and that the evolution of the "modern avian" digestive system in paravians was highly homoplastic [14]. A preliminary Jehol food web is reconstructed from current data.
Topics: Animal Nutritional Physiological Phenomena; Animals; Diet; Digestion; Dinosaurs; Food Chain; Lizards
PubMed: 31303494
DOI: 10.1016/j.cub.2019.06.020 -
Journal of Animal Science Jan 2019A combination of in vivo (ileal cannulated pigs) and in vitro (fecal inoculum-based incubation) methodologies were used to predict the effects of dietary supplementation...
A combination of in vivo (ileal cannulated pigs) and in vitro (fecal inoculum-based incubation) methodologies were used to predict the effects of dietary supplementation with soluble or insoluble dietary fiber on hindgut VFA production and absorption. Energy contribution from hindgut VFA and apparent ileal (AID) and total tract (ATTD) digestibility of energy and DM was also investigated. Twelve ileal cannulated Genesus barrows (initial BW: 35.1 ± 0.44 kg) were allocated to 1 of the 3 corn-soybean meal-based diets without (control), or with flaxseed meal (FM) or oat hulls (OH) in a 2-period cross-over design. Flaxseed meal and oat hulls were used as sources of soluble and insoluble fiber, respectively. In each period, 4 pigs were offered 1 of the 3 diets, for 12 d followed by fecal (day 13 and 14) and ileal digesta collection (day 15 and 16) (n = 8). Ileal digesta were collected, freeze-dried, and subjected to in vitro fermentation using fecal inoculum, to predict production and absorption of VFA and energy production, and digestibility of DM and energy. The quantity of acetic, propionic, butyric, and valeric acids produced by in vitro fermentation was higher (P < 0.05) for the diet containing flaxseed meal compared with the control and OH diets. The predicted quantity of VFA produced and absorbed in the hindgut was greater (P < 0.05) in pigs that consumed the FM diet than those fed the control or OH diet. Pigs fed the control diet had greater (P < 0.05) AID and ATTD of DM than pigs offered the OH or FM diet. The determined disappearance of DM was lower (P < 0.05) in pigs fed the control and OH diets than in pigs that consumed the FM diet. The quantity of digested energy in the upper gut was reduced (P < 0.05) more in pigs fed the OH diet than in pigs fed the FM diet. The consumption of the FM diet increased (P < 0.05) the quantity of digested energy, energy produced and absorbed from VFA in the hindgut, and the percentage contribution of VFA from fermentation to total tract digestible energy, compared with the control and OH diets. In conclusion, dietary supplementation with insoluble fiber from oat hulls reduced ileal digested energy more than soluble fiber from flaxseed meal. Addition of soluble fiber to pig diets increased the energy contribution from VFA produced by hindgut fermentation to the total tract digestible energy, compared with dietary addition of insoluble fiber.
Topics: Animal Feed; Animals; Avena; Cross-Over Studies; Diet; Dietary Fiber; Digestion; Fatty Acids, Volatile; Feces; Fermentation; Flax; Gastrointestinal Tract; Ileum; Male; Glycine max; Swine
PubMed: 30321361
DOI: 10.1093/jas/sky399