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Reviews in Endocrine & Metabolic... Jun 2023Measurement of phase angle using bioimpedance analysis (BIA) has become popular as an index of so-called "cellular health". What precisely is meant by this term is not... (Review)
Review
Measurement of phase angle using bioimpedance analysis (BIA) has become popular as an index of so-called "cellular health". What precisely is meant by this term is not always clear but strong relationships have been found between cellular water status (the relative amounts of extra- and intracellular water), cell membrane integrity and cellular mass. Much of the current research is empirical observation and frequently pays little regard to the underlying biophysical models that underpin the BIA technique or attempts to provide mechanistic explanations for the observations. This brief review seeks to provide a basic understanding of the electrical models frequently used to describe the passive electrical properties of tissues with particular focus on phase angle. In addition, it draws attention to some practical concerns in the measurement of phase angle and notes the additional understanding that can be gained when phase angle are obtained with bioimpedance spectroscopy (BIS) rather than single frequency BIA (SFBIA) along with the potential for simulation modelling.
Topics: Humans; Electric Impedance; Water
PubMed: 36749540
DOI: 10.1007/s11154-022-09780-3 -
Critical Care (London, England) Oct 2018Acute respiratory distress syndrome (ARDS) is a clinical entity that acutely affects the lung parenchyma, and is characterized by diffuse alveolar damage and increased... (Review)
Review
Acute respiratory distress syndrome (ARDS) is a clinical entity that acutely affects the lung parenchyma, and is characterized by diffuse alveolar damage and increased pulmonary vascular permeability. Currently, computed tomography (CT) is commonly used for classifying and prognosticating ARDS. However, performing this examination in critically ill patients is complex, due to the need to transfer these patients to the CT room. Fortunately, new technologies have been developed that allow the monitoring of patients at the bedside. Electrical impedance tomography (EIT) is a monitoring tool that allows one to evaluate at the bedside the distribution of pulmonary ventilation continuously, in real time, and which has proven to be useful in optimizing mechanical ventilation parameters in critically ill patients. Several clinical applications of EIT have been developed during the last years and the technique has been generating increasing interest among researchers. However, among clinicians, there is still a lack of knowledge regarding the technical principles of EIT and potential applications in ARDS patients. The aim of this review is to present the characteristics, technical concepts, and clinical applications of EIT, which may allow better monitoring of lung function during ARDS.
Topics: Electric Impedance; Humans; Lung; Respiration, Artificial; Respiratory Distress Syndrome; Respiratory Physiological Phenomena; Tomography, X-Ray Computed
PubMed: 30360753
DOI: 10.1186/s13054-018-2195-6 -
Current Opinion in Critical Care Aug 2021Insight into body composition is of great value in the ICU. Bioelectric impedance analysis (BIA) is the most applicable bedside technique. However, bioimpedance has not... (Review)
Review
PURPOSE OF REVIEW
Insight into body composition is of great value in the ICU. Bioelectric impedance analysis (BIA) is the most applicable bedside technique. However, bioimpedance has not been validated in the critically ill, and the interpretation of the measurements poses challenges. This review discusses the potential clinical applications of BIA and explores caveats and solutions to its use in the intensive care setting.
RECENT FINDINGS
A correlation is repeatedly found between raw impedance parameters, fluid ratios, overhydration, and adverse outcome of critical illness. However, cut-off and reference values remain elusive. Experience with BIA-guided fluid management in the ICU is limited. BIA-derived muscle mass appears a promising biomarker for sarcopenia, correlating well with CT-analysis. Body cell mass and fat-free mass provide potential use in estimation of metabolic rate, protein requirements and pharmacokinetics. Several methods of reducing bias in BIA parameters in critical illness require validation.
SUMMARY
There are currently too many uncertainties and discrepancies regarding interpretation of bioimpedance in critical illness, to justify therapeutic consequences. However, there are several promising areas of research, concerning some of the most urgent clinical problems in intensive care, emphasizing the need to evaluate further the use and interpretation of bioimpedance in the intensive care setting.
Topics: Body Composition; Critical Care; Critical Illness; Electric Impedance; Humans; Water-Electrolyte Imbalance
PubMed: 33967207
DOI: 10.1097/MCC.0000000000000840 -
Sensors (Basel, Switzerland) Oct 2022Electric impedance spectroscopy is an alternative technology to existing methods that shows promising results in the agro-food industry and plant physiology research....
Electric impedance spectroscopy is an alternative technology to existing methods that shows promising results in the agro-food industry and plant physiology research. For example, this technology makes it possible to monitor the condition of plants, even in the early stages of development, and to control the quality of finished products. However, the use of electric impedance spectroscopy is often associated with the need to organize special laboratory conditions for measurements. Our aim is to extract information about the state of health of the internal tissues of a plant's branches from impedance measurements. Therefore, we propose a new technique using the device and model developed by us that makes it possible to monitor the condition of tree branch tissues in situ. An apple tree was chosen as the object under study, and the dependence of the impedance of the apple tree branch on the signal frequency and branch length was analyzed. The change in the impedance of an apple tree branch during drying was also analyzed. It was shown that, when a branch dries out, the conductivity of the xylem mainly decreases. The developed technique was also applied to determine the development of the vascular system of an apple tree after grafting. It was shown that the processing of the scion and rootstock sections with the help of cold atmospheric plasma and a plasma-treated solution contributes to a better formation of graft unions.
Topics: Trees; Plant Roots; Dielectric Spectroscopy; Malus; Electric Impedance
PubMed: 36366006
DOI: 10.3390/s22218310 -
Sensors (Basel, Switzerland) Sep 2020Biosensors based on magneto-impedance (MI) effect are powerful tools for biomedical applications as they are highly sensitive, stable, exhibit fast response, small in... (Review)
Review
Biosensors based on magneto-impedance (MI) effect are powerful tools for biomedical applications as they are highly sensitive, stable, exhibit fast response, small in size, and have low hysteresis and power consumption. However, the performance of these biosensors is influenced by a variety of factors, including the design, geometry, materials and fabrication procedures. Other less appreciated factors influencing the MI effect include measuring circuit implementation, the material used for construction, geometry of the thin film sensing element, and patterning shapes compatible with the interface microelectronic circuitry. The type magnetic (ferrofluid, Dynabeads, and nanoparticles) and size of the particles, the magnetic particle concentration, magnetic field strength and stray magnetic fields can also affect the sensor sensitivity. Based on these considerations it is proposed that ideal MI biosensor sensitivity could be achieved when the sensor is constructed in sandwich thick magnetic layers with large sensing area in a meander shape, measured with circuitry that provides the lowest possible external inductance at high frequencies, enclosed by a protective layer between magnetic particles and sensing element, and perpendicularly magnetized when detecting high-concentration of magnetic particles.
Topics: Biosensing Techniques; Electric Impedance; Magnetic Fields; Magnetics; Nanoparticles
PubMed: 32932740
DOI: 10.3390/s20185213 -
Biosensors Nov 2021Cellular heterogeneity is of significance in cell-based assays for life science, biomedicine and clinical diagnostics. Electrical impedance sensing technology has become... (Review)
Review
Cellular heterogeneity is of significance in cell-based assays for life science, biomedicine and clinical diagnostics. Electrical impedance sensing technology has become a powerful tool, allowing for rapid, non-invasive, and label-free acquisition of electrical parameters of single cells. These electrical parameters, i.e., equivalent cell resistance, membrane capacitance and cytoplasm conductivity, are closely related to cellular biophysical properties and dynamic activities, such as size, morphology, membrane intactness, growth state, and proliferation. This review summarizes basic principles, analytical models and design concepts of single-cell impedance sensing devices, including impedance flow cytometry (IFC) to detect flow-through single cells and electrical impedance spectroscopy (EIS) to monitor immobilized single cells. Then, recent advances of both electrical impedance sensing systems applied in cell recognition, cell counting, viability detection, phenotypic assay, cell screening, and other cell detection are presented. Finally, prospects of impedance sensing technology in single-cell analysis are discussed.
Topics: Dielectric Spectroscopy; Electric Impedance; Flow Cytometry; Single-Cell Analysis; Technology
PubMed: 34821686
DOI: 10.3390/bios11110470 -
Biophysical Journal Mar 2022Electric phenomena in brain tissue can be measured using extracellular potentials, such as the local field potential, or the electro-encephalogram. The interpretation of...
Electric phenomena in brain tissue can be measured using extracellular potentials, such as the local field potential, or the electro-encephalogram. The interpretation of these signals depends on the electric structure and properties of extracellular media, but the measurements of these electric properties are still debated. Some measurements point to a model in which the extracellular medium is purely resistive, and thus parameters such as electric conductivity and permittivity should be independent of frequency. Other measurements point to a pronounced frequency dependence of these parameters, with scaling laws that are consistent with capacitive or diffusive effects. However, these experiments correspond to different preparations, and it is unclear how to correctly compare them. Here, we provide for the first time, impedance measurements (in the 1-10 kHz frequency range) using the same setup in various preparations, from primary cell cultures to acute brain slices, and a comparison with similar measurements performed in artificial cerebrospinal fluid with no biological material. The measurements show that when the current flows across a cell membrane, the frequency dependence of the macroscopic impedance between intracellular and extracellular electrodes is significant, and cannot be captured by a model with resistive media. Fitting a mean-field model to the data shows that this frequency dependence could be explained by the ionic diffusion mainly associated with Debye layers surrounding the membranes. We conclude that neuronal membranes and their ionic environment induce strong deviations to resistivity that should be taken into account to correctly interpret extracellular potentials generated by neurons.
Topics: Brain; Electric Conductivity; Electric Impedance; Electrodes; Neurons
PubMed: 35182541
DOI: 10.1016/j.bpj.2022.02.022 -
Arquivos de Gastroenterologia 2016
Topics: Electric Impedance; Esophageal pH Monitoring; Gastroesophageal Reflux; Humans
PubMed: 27438414
DOI: 10.1590/S0004-28032016000300001 -
Sensors (Basel, Switzerland) Feb 2021Organ-on-chip devices have provided the pharmaceutical and tissue engineering worlds much hope since they arrived and began to grow in sophistication. However,... (Review)
Review
Organ-on-chip devices have provided the pharmaceutical and tissue engineering worlds much hope since they arrived and began to grow in sophistication. However, limitations for their applicability were soon realized as they lacked real-time monitoring and sensing capabilities. The users of these devices relied solely on endpoint analysis for the results of their tests, which created a chasm in the understanding of life between the lab the natural world. However, this gap is being bridged with sensors that are integrated into organ-on-chip devices. This review goes in-depth on different sensing methods, giving examples for various research on mechanical, electrical resistance, and bead-based sensors, and the prospects of each. Furthermore, the review covers works conducted that use specific sensors for oxygen, and various metabolites to characterize cellular behavior and response in real-time. Together, the outline of these works gives a thorough analysis of the design methodology and sophistication of the current sensor integrated organ-on-chips.
Topics: Electric Impedance; Lab-On-A-Chip Devices; Oligonucleotide Array Sequence Analysis
PubMed: 33671996
DOI: 10.3390/s21041367 -
Sensors (Basel, Switzerland) Mar 2020This research offers a method for separating the components of tissue impedance, namely resistance and capacitive reactance. Two objects that have similar impedance or...
This research offers a method for separating the components of tissue impedance, namely resistance and capacitive reactance. Two objects that have similar impedance or low contrast can be improved through separating the real and imaginary images. This method requires an Electrical Impedance Tomography (EIT) device. EIT can obtain potential data and the phase angle between the current and the potential measured. In the future, the device is very suitable for imaging organs in the thorax and abdomen that have the same impedance but different resistance and capacitive reactance. This device consists of programmable generators, Voltage Controlled Current Source (VCCS), mulptiplexer-demultiplexer potential meters, and phase meters. Data collecting was done by employing neighboring, while reconstruction was used the linear back-projection method from two different data frequencies, namely 10 kHz and 100 kHz. Phantom used in this experiment consists of distillated water and a carrot as an anomaly. Potential and phase data from the device is reconstructed to produce impedance, real, and imaginary images. Image analysis is performed by comparing the three images to the phantom. The experimental results show that the device is reliable.
Topics: Algorithms; Electric Impedance; Humans; Image Processing, Computer-Assisted; Phantoms, Imaging; Thorax; Tomography
PubMed: 32235454
DOI: 10.3390/s20071907