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Micromachines Jan 2019Phenomena of electrically driven fluid flows, known as electro-osmosis, and particle transport in a liquid electrolyte, known as electrophoresis, collectively form a... (Review)
Review
Phenomena of electrically driven fluid flows, known as electro-osmosis, and particle transport in a liquid electrolyte, known as electrophoresis, collectively form a subject of electrokinetics. Electrokinetics shows a great potential in microscopic manipulation of matter for various scientific and technological applications. Electrokinetics is usually studied for isotropic electrolytes. Recently it has been demonstrated that replacement of an isotropic electrolyte with an anisotropic, or liquid crystal (LC), electrolyte, brings about entirely new mechanisms of spatial charge formation and electrokinetic effects. This review presents the main features of liquid crystal-enabled electrokinetics (LCEK) rooted in the field-assisted separation of electric charges at deformations of the director that describes local molecular orientation of the LC. Since the electric field separates the charges and then drives the charges, the resulting electro-osmotic and electrophoretic velocities grow as the square of the applied electric field. We describe a number of related phenomena, such as alternating current (AC) LC-enabled electrophoresis of colloidal solid particles and fluid droplets in uniform and spatially-patterned LCs, swarming of colloids guided by photoactivated surface patterns, control of LCEK polarity through the material properties of the LC electrolyte, LCEK-assisted mixing at microscale, separation and sorting of small particles. LC-enabled electrokinetics brings a new dimension to our ability to manipulate dynamics of matter at small scales and holds a major promise for future technologies of microfluidics, pumping, mixing, sensing, and diagnostics.
PubMed: 30634568
DOI: 10.3390/mi10010045 -
Electrophoresis Apr 2021Electroosmotic flow (EOF), a consequence of an imposed electric field onto an electrolyte solution in the tangential direction of a charged surface, has emerged as an... (Review)
Review
Electroosmotic flow (EOF), a consequence of an imposed electric field onto an electrolyte solution in the tangential direction of a charged surface, has emerged as an important phenomenon in electrokinetic transport at the micro/nanoscale. Because of their ability to efficiently pump liquids in miniaturized systems without incorporating any mechanical parts, electroosmotic methods for fluid pumping have been adopted in versatile applications-from biotechnology to environmental science. To understand the electrokinetic pumping mechanism, it is crucial to identify the role of an ionically polarized layer, the so-called electrical double layer (EDL), which forms in the vicinity of a charged solid-liquid interface, as well as the characteristic length scale of the conducting media. Therefore, in this tutorial review, we summarize the development of electrical double layer models from a historical point of view to elucidate the interplay and configuration of water molecules and ions in the vicinity of a solid-liquid interface. Moreover, we discuss the physicochemical phenomena owing to the interaction of electrical double layer when the characteristic length of the conducting media is decreased from the microscale to the nanoscale. Finally, we highlight the pioneering studies and the most recent works on electro osmotic flow devoted to both theoretical and experimental aspects.
Topics: Electroosmosis; Ions; Microfluidics
PubMed: 33382088
DOI: 10.1002/elps.202000313 -
Animal Models and Experimental Medicine Apr 2023Understanding the bioelectrical properties of bone tissue is key to developing new treatment strategies for bone diseases and injuries, as well as improving the design... (Review)
Review
Understanding the bioelectrical properties of bone tissue is key to developing new treatment strategies for bone diseases and injuries, as well as improving the design and fabrication of scaffold implants for bone tissue engineering. The bioelectrical properties of bone tissue can be attributed to the interaction of its various cell lineages (osteocyte, osteoblast and osteoclast) with the surrounding extracellular matrix, in the presence of various biomechanical stimuli arising from routine physical activities; and is best described as a combination and overlap of dielectric, piezoelectric, pyroelectric and ferroelectric properties, together with streaming potential and electro-osmosis. There is close interdependence and interaction of the various electroactive and electrosensitive components of bone tissue, including cell membrane potential, voltage-gated ion channels, intracellular signaling pathways, and cell surface receptors, together with various matrix components such as collagen, hydroxyapatite, proteoglycans and glycosaminoglycans. It is the remarkably complex web of interactive cross-talk between the organic and non-organic components of bone that define its electrophysiological properties, which in turn exerts a profound influence on its metabolism, homeostasis and regeneration in health and disease. This has spurred increasing interest in application of electroactive scaffolds in bone tissue engineering, to recapitulate the natural electrophysiological microenvironment of healthy bone tissue to facilitate bone defect repair.
Topics: Tissue Scaffolds; Bone Regeneration; Tissue Engineering; Bone and Bones; Durapatite
PubMed: 36856186
DOI: 10.1002/ame2.12300 -
JASA Express Letters Aug 2021Electro-osmosis (EO) is a non-traditional pumping and transduction mechanism with the ability to project acoustic energy in fluids. This investigation experimentally...
Electro-osmosis (EO) is a non-traditional pumping and transduction mechanism with the ability to project acoustic energy in fluids. This investigation experimentally validates the influence of zeta potential, a well-studied physical characteristic used for quantifying the efficacy of an EO pump, on generation of sound pressure level. Acoustic signals of discrete frequencies were observed from 130 Hz to over 150 kHz. EO-type projectors are an attractive technology in that it does not contain moving parts, can be fabricated using a variety of materials, is intrinsically resilient to effects of hydrostatic pressure, and may be designed on the micro-electromechanical system scale.
Topics: Acoustics; Osmosis
PubMed: 36154251
DOI: 10.1121/10.0005763 -
Biomechanics and Modeling in... Apr 2021We propose a continuum finite strain theory for the interplay between the bioelectricity and the poromechanics of a cell cluster. Specifically, we refer to a cluster of...
We propose a continuum finite strain theory for the interplay between the bioelectricity and the poromechanics of a cell cluster. Specifically, we refer to a cluster of closely packed cells, whose mechanics is governed by a polymer network of cytoskeletal filaments joined by anchoring junctions, modeled through compressible hyperelasticity. The cluster is saturated with a solution of water and ions. We account for water and ion transport in the intercellular spaces, between cells through gap junctions, and across cell membranes through aquaporins and ion channels. Water fluxes result from the contributions due to osmosis, electro-osmosis, and water pressure, while ion fluxes encompass electro-diffusive and convective terms. We consider both the cases of permeable and impermeable cluster boundary, the latter simulating the presence of sealing tight junctions. We solve the coupled governing equations for a one-dimensional axisymmetric benchmark through finite elements, thus determining the spatiotemporal evolution of the intracellular and extracellular ion concentrations, setting the membrane potential, and water concentrations, establishing the cluster deformation. When suitably complemented with genetic, biochemical, and growth dynamics, we expect this model to become a useful instrument for investigating specific aspects of developmental mechanobioelectricity.
Topics: Biomechanical Phenomena; Cells; Electrophysiological Phenomena; Gap Junctions; Intracellular Space; Models, Biological; Osmosis; Thermodynamics; Tight Junctions
PubMed: 33145723
DOI: 10.1007/s10237-020-01399-0 -
Cells & Development Dec 2021Lumen formation plays an essential role in the morphogenesis of tissues during development. Here we review the physical principles that play a role in the growth and... (Review)
Review
Lumen formation plays an essential role in the morphogenesis of tissues during development. Here we review the physical principles that play a role in the growth and coarsening of lumens. Solute pumping by the cell, hydraulic flows driven by differences of osmotic and hydrostatic pressures, balance of forces between extracellular fluids and cell-generated cytoskeletal forces, and electro-osmotic effects have been implicated in determining the dynamics and steady-state of lumens. We use the framework of linear irreversible thermodynamics to discuss the relevant force, time and length scales involved in these processes. We focus on order of magnitude estimates of physical parameters controlling lumen formation and coarsening.
Topics: Cytoskeleton; Extracellular Fluid; Morphogenesis; Osmosis; Physics
PubMed: 34339904
DOI: 10.1016/j.cdev.2021.203724 -
3D Printing and Additive Manufacturing Aug 2022Layered assembly is a voxel-based additive manufacturing process that relies on parallel grasping of voxels to produce multi-material parts. Although there exists...
Layered assembly is a voxel-based additive manufacturing process that relies on parallel grasping of voxels to produce multi-material parts. Although there exists substantial diversity in mechanisms of gripping, there still exists a lack of consistency, accuracy, and efficacy in positioning very large numbers of milli-, micro-, and nano-scale objects. We demonstrate the use of parallel electro-osmotic grippers to selectively transport multiple millimeter-sized voxels simultaneously. In contrast to previous research focused on using arrays of droplets to grab a single substrate, each element in the array is individually controlled via capillary effects, which are, in turn, controlled by an electric field to create predetermined patterns of droplets to pick and place selected objects. The demonstrated fluidic pick-and-place method has two key advantages: It is suitable for transport of fragile and complex objects due to the lack of mechanical contact, and it easily parallelizes to arbitrary array sizes for massively parallel pick-and-place. This work demonstrates a 25-element parallel assembly of 1.5-mm spheres with 95-98% grasping reliability, in a variety of geometric patterns. Experimental performance was validated against both analytical and computational models. The results suggest that electro-osmotic droplet arrays may enable the additive manufacturing of multi-material objects containing millions of components in the same print bed.
PubMed: 36660230
DOI: 10.1089/3dp.2020.0224 -
Membranes Mar 2021Climate change, population growth, and increased industrial activities are exacerbating freshwater scarcity and leading to increased interest in desalination of saline... (Review)
Review
Climate change, population growth, and increased industrial activities are exacerbating freshwater scarcity and leading to increased interest in desalination of saline water. Brackish water is an attractive alternative to freshwater due to its low salinity and widespread availability in many water-scarce areas. However, partial or total desalination of brackish water is essential to reach the water quality requirements for a variety of applications. Selection of appropriate technology requires knowledge and understanding of the operational principles, capabilities, and limitations of the available desalination processes. Proper combination of feedwater technology improves the energy efficiency of desalination. In this article, we focus on pressure-driven and electro-driven membrane desalination processes. We review the principles, as well as challenges and recent improvements for reverse osmosis (RO), nanofiltration (NF), electrodialysis (ED), and membrane capacitive deionization (MCDI). RO is the dominant membrane process for large-scale desalination of brackish water with higher salinity, while ED and MCDI are energy-efficient for lower salinity ranges. Selective removal of multivalent components makes NF an excellent option for water softening. Brackish water desalination with membrane processes faces a series of challenges. Membrane fouling and scaling are the common issues associated with these processes, resulting in a reduction in their water recovery and energy efficiency. To overcome such adverse effects, many efforts have been dedicated toward development of pre-treatment steps, surface modification of membranes, use of anti-scalant, and modification of operational conditions. However, the effectiveness of these approaches depends on the fouling propensity of the feed water. In addition to the fouling and scaling, each process may face other challenges depending on their state of development and maturity. This review provides recent advances in the material, architecture, and operation of these processes that can assist in the selection and design of technologies for particular applications. The active research directions to improve the performance of these processes are also identified. The review shows that technologies that are tunable and particularly efficient for partial desalination such as ED and MCDI are increasingly competitive with traditional RO processes. Development of cost-effective ion exchange membranes with high chemical and mechanical stability can further improve the economy of desalination with electro-membrane processes and advance their future applications.
PubMed: 33805438
DOI: 10.3390/membranes11040246 -
IScience Oct 2021This paper provides a perspective on potential applications of a new single-molecule technique, viz., the nanopore electro-osmotic trap (NEOtrap). This solid-state... (Review)
Review
This paper provides a perspective on potential applications of a new single-molecule technique, viz., the nanopore electro-osmotic trap (NEOtrap). This solid-state nanopore-based method uses locally induced electro-osmosis to form a hydrodynamic trap for single molecules. Ionic current recordings allow one to study an unlabeled protein or nanoparticle of arbitrary charge that can be held in the nanopore's most sensitive region for very long times. After motivating the need for improved single-molecule technologies, we sketch various possible technical extensions and combinations of the NEOtrap. We lay out diverse applications in biosensing, enzymology, protein folding, protein dynamics, fingerprinting of proteins, detecting post-translational modifications, and all that at the level of single proteins - illustrating the unique versatility and potential of the NEOtrap.
PubMed: 34755079
DOI: 10.1016/j.isci.2021.103007 -
Analytical Chemistry Dec 2021Nanopipettes are finding increasing use as nano "test tubes", with reactions triggered through application of an electrochemical potential between electrodes in the...
Nanopipettes are finding increasing use as nano "test tubes", with reactions triggered through application of an electrochemical potential between electrodes in the nanopipette and a bathing solution (bath). Key to this application is an understanding of how the applied potential induces mixing of the reagents from the nanopipette and the bath. Here, we demonstrate a laser scanning confocal microscope (LSCM) approach to tracking the ingress of dye into a nanopipette (20-50 nm diameter end opening). We examine the case of dianionic fluorescein under alkaline conditions (pH 11) and large applied tip potentials (±10 V), with respect to the bath, and surprisingly find that dye ingress from the bath into the nanopipette is not observed under either sign of potential. Finite element method (FEM) simulations indicate this is due to the dominance of electro-osmosis in mass transport, with electro-osmotic flow in the conventional direction at +10 V and electro-osmosis of the second kind acting in the same direction at -10 V, caused by the formation of significant space charge in the center of the orifice. The results highlight the significant deviation in mass transport behavior that emerges at the nanoscale and the utility of the combined LSCM and FEM approach in deepening understanding, which in turn should promote new applications of nanopipettes.
Topics: Microscopy, Confocal; Osmosis
PubMed: 34846865
DOI: 10.1021/acs.analchem.1c02371