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Infection, Genetics and Evolution :... Mar 2022Empedobacter falsenii is an emerging opportunistic pathogen that has been occasionally implicated in various human infections. In this study, we described the genomic...
Empedobacter falsenii is an emerging opportunistic pathogen that has been occasionally implicated in various human infections. In this study, we described the genomic features of a multidrug resistant E. falsenii Q1655 obtained from a patient attending a public hospital in Sokoto, northwest Nigeria. The isolate, E. falsenii Q1655, was isolated from the stool sample of a patient in Sokoto, Nigeria. The identity of the isolate was confirmed by MALDITOF-MS. The disc diffusion test and modified Carba-NP test were used for phenotypic antibiotic susceptibility test and carbapenemase enzyme production test, respectively. The whole genome of the strain was sequenced using the Illumina MiSeq technique. Resistome analysis was done by annotation of the WGS against the ARG-ANNOT database. The isolate was resistant to all β-lactam antibiotics with the exception of cefepime. The MICs of imipenem and ertapenem as determined by E-test were 12 μg/ml and 2 μg/ml, respectively. Modified Carba NP test showed that the strain was carbapenemase producing. Resistome analysis revealed the presence of a novel metallo-β-lactamase, a chromosomal bla, which exhibited 94.92% and 97.02% nucleotide and protein sequence identities respectively with bla gene of E. falsenii 174,820. Seven and eight amino-acid substitutions were observed with the bla and bla, respectively. We reported the first isolation and genomic description of an extensively drug resistant isolate of Empedobacter falsenii in Nigeria. This report broadens our knowledge of carbapenem resistance in E. falsenii and it will serve as a useful guide in the development of antibiotic use policy.
Topics: Anti-Bacterial Agents; Ertapenem; Flavobacteriaceae; Genome, Bacterial; Imipenem; Microbial Sensitivity Tests; beta-Lactamases
PubMed: 35121093
DOI: 10.1016/j.meegid.2022.105234 -
IDCases 2023, formerly known as Wautersiella falsenii, was first described in 2006. It is a non-motile, non-fermenting, gram-negative rod, which grows aerobically. A handful of case...
, formerly known as Wautersiella falsenii, was first described in 2006. It is a non-motile, non-fermenting, gram-negative rod, which grows aerobically. A handful of case reports have described its isolation from respiratory, urinary and abscess samples. Besides clinical specimens, it has also been isolated from metalworking fluids and aerosols, carpet surfaces and polluted soils. However, to our knowledge, this is the first case report that describes bacteremia from . We present a 56-year-old male with liver cirrhosis, congestive heart failure and substance abuse disorder, who was admitted in a community hospital in the Bronx, New York for bacteremia of the said organism. This bacterium may serve as a reservoir for resistance genes, such as ERB, tetX and aadS, posing dangers to immunocompromised or hospitalized patients, highlighting the need to study this organism further.
PubMed: 37645528
DOI: 10.1016/j.idcr.2023.e01814 -
Polish Journal of Microbiology Feb 2022is a rarely non-fermenting Gram-negative bacterium and belongs to the family. This nosocomial pathogen can cause several human infections, especially among...
is a rarely non-fermenting Gram-negative bacterium and belongs to the family. This nosocomial pathogen can cause several human infections, especially among immunocompromised patients. Here, we describe the whole genome sequence of a clinical strain isolated from a urine sample of a 35-year-old woman with a urinary tract infection in Tunisia. We investigated its phenotype and genotype. After bacterial identification by the MALDI-TOF method, the whole-genome sequencing of this strain was performed. This isolate was not susceptible to various antibiotics, including β-lactams, aminoglycosides, and quinolones. However, it remains susceptible to imipenem (MIC = 0.25 mg/l), ertapenem (MIC = 0.75 mg/l), and meropenem (MIC = 0.19 mg/l). Interestingly, the E-TEST (MP/MPI) showed a reduced MIC of meropenem +/- EDTA (0.064 μg/ml). Besides, the color change from yellow to red in the β CARBA test only after 24 hours of incubation can be interpreted in two ways. On the one hand, as a likely low expression of the gene encoding metallo-β-lactamase. On the other hand, and more likely, it may be a false-positive result because, according to the test manufacturer's recommendations, the test should be read after 30 minutes. Perhaps, therefore, this gene is not expressed in the tested strain. Moreover, the whole-genome sequence analysis demonstrated the presence of a novel chromosomally located subclass B1 metallo-β-lactamase EBR-like enzyme, sharing 94.92% amino acid identity with a previously described carbapenemase produced by , EBR-1. The results also showed the detection of other antibiotic resistance genes and the absence of plasmids. So far, this study is the first report on the detection of in Tunisia. These findings prove that could be a potential reservoir of antibiotic resistance genes, e.g., β-lactamases. Collaborative efforts and effective hygiene measures should be established to prevent the emergence of this species in our health care settings.
Topics: Anti-Bacterial Agents; Flavobacteriaceae; Humans; Meropenem; Microbial Sensitivity Tests; Tunisia; Urinary Tract Infections; beta-Lactamases
PubMed: 35635163
DOI: 10.33073/pjm-2022-010 -
The Journal of Veterinary Medical... Nov 2019The aim of the present study was to evaluate the bacterial flora in the udder and intestinal environments in cows with and without protothecal mastitis. We used...
The aim of the present study was to evaluate the bacterial flora in the udder and intestinal environments in cows with and without protothecal mastitis. We used next-generation sequencing (NGS) analysis to identify 16S rRNA genes from bacterial flora present in 13 milk and 13 fecal samples from protothecal mastitic and healthy dairy cows in the Aichi region of Japan. Sequences associated with 5 species (Calothrix desertica, Corynebacterium simulans, Corynebacterium striatum, Empedobacter falsenii, and Rothia endophytica) showed the highest prevalence in samples of milk and feces from animals with protothecal mastitis. This range of species differed from those detected in the milk and feces from healthy cows.
Topics: Animals; Cattle; Feces; Female; High-Throughput Nucleotide Sequencing; Mastitis, Bovine; RNA, Bacterial; RNA, Ribosomal, 16S
PubMed: 30918225
DOI: 10.1292/jvms.18-0649