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Clinical Microbiology and Infection :... Sep 2002To investigate the natural susceptibility to 69 antimicrobial agents of 107 Enterobacter strains comprising E. amnigenus (n = 18), E. cancerogenus (n = 26), E. gergoviae...
OBJECTIVE
To investigate the natural susceptibility to 69 antimicrobial agents of 107 Enterobacter strains comprising E. amnigenus (n = 18), E. cancerogenus (n = 26), E. gergoviae (n = 28) and E. sakazakii (n = 35).
METHODS
Minimal inhibitory concentrations (MICs) were determined with a microdilution procedure in Isosensitest broth and cation-adjusted Mueller-Hinton broth.
RESULTS
All the species were naturally sensitive or intermediate to tetracyclines, amino-glycosides, numerous beta-lactams (acylureidopenicillins, ticarcillin, ampicillin/sulbactam, several cephalosporins, carbapenems, aztreonam), quinolones, antifolates, chloramphenicol and nitrofurantoin. Natural resistance was found to penicillin G, oxacillin, several macrolides, lincosamides, streptogramins, glycopeptides, rifampicin and fusidic acid. Species-related differences in natural susceptibility were found to some beta-lactams, azithromycin and fosfomycin. Whereas E. gergoviae was the most susceptible species to azithromycin, E. cancerogenus was most susceptible to fosfomycin and was the only species showing natural resistance to amoxicillin, amoxicillin/clavulanic acid, cefaclor, cefazoline, loracarbef and cefoxitin. There were only minor medium-dependent differences in susceptibility to most antibiotics.
CONCLUSIONS
The present study establishes a database concerning the natural susceptibility of recently established Enterobacter species to a wide range of antibiotics, which can be applied for the validation of routine susceptibility test results. beta-Lactam susceptibility patterns indicate the expression of species-specific beta-lactamases expressed at high or low levels in all the species except E. sakazakii.
Topics: Anti-Bacterial Agents; Databases, Factual; Drug Resistance, Bacterial; Enterobacter; Humans; Lactams; Microbial Sensitivity Tests
PubMed: 12427217
DOI: 10.1046/j.1469-0691.2002.00413.x -
Internal Medicine (Tokyo, Japan) Jan 2018We herein describe a case of trauma-related wound infection with a subcutaneous abscess caused by both Enterobacter cancerogenus and Aeromonas hydrophila. An 89-year-old...
We herein describe a case of trauma-related wound infection with a subcutaneous abscess caused by both Enterobacter cancerogenus and Aeromonas hydrophila. An 89-year-old Japanese man was admitted to our hospital because of an injury that he had suffered in a car accident. The right dorsal region of the foot around the wound was reddish and swelling. The pus culture on his right foot grew E. cancerogenus and A. hydrophila. The patient was successfully treated with a 10-day course of meropenem and a 25-day course of levofloxacin. E. cancerogenus can therefore be a causative pathogen in skin and soft tissue infections among trauma patients.
Topics: Aeromonas hydrophila; Aged, 80 and over; Anti-Bacterial Agents; Asian People; Enterobacter; Gram-Negative Bacterial Infections; Humans; Levofloxacin; Male; Meropenem; Thienamycins; Treatment Outcome; Wound Infection
PubMed: 29033439
DOI: 10.2169/internalmedicine.9171-17 -
Foods (Basel, Switzerland) Dec 2021The consumption of non-dairy milk is on the rise due to health benefits. Although there is increasing inclination towards milk alternatives (MA), there is limited data...
The consumption of non-dairy milk is on the rise due to health benefits. Although there is increasing inclination towards milk alternatives (MA), there is limited data on antibiotic resistant bacteria in these substitutes. The aim of this study was to investigate antimicrobial resistance of bacteria isolated from MA. A total of 138 extracts from almonds ( = 63), cashew nuts ( = 36), and soybeans ( = 39) were analyzed for . The identification of the bacteria was based on biochemical and PCR methods. Antibiotic sensitivity was determined by using the Kirby-Bauer disk diffusion technique. Overall, 31% (43 of 138) of extracts were positive for Ten bacterial species were identified, of which (42.7%) and (35.4%) were the most predominant species ( < 0.05). Antibiotic resistance was exhibited to vancomycin (88.3%), novobiocin (83.8%), erythromycin (81.1%), which was significantly higher ( < 0.05) than in tetracycline (59.5%), cefpodoxime (30.6%), and nalidixic acid (6.3%). There was no resistance displayed to kanamycin and imipenem. ERY-NOV-VAN-TET and ERY-NOV-CEP-VAN-TET were the most common resistant patterns displayed by The findings of this study suggest that MAs, though considered healthy, may be a reservoir of multidrug resistant opportunist pathogens.
PubMed: 34945621
DOI: 10.3390/foods10123070 -
Research in Microbiology Sep 1989Strains labelled Enterobacter cancerogenus (Erwinia cancerogena) and strains labelled Enterobacter taylorae were found to constitute a single DNA-relatedness group (S1...
Strains labelled Enterobacter cancerogenus (Erwinia cancerogena) and strains labelled Enterobacter taylorae were found to constitute a single DNA-relatedness group (S1 nuclease hybridization method). Furthermore, no phenotypic test among the conventional and nutritional tests performed could differentiate Enterobacter cancerogenus from Enterobacter taylorae. Therefore, Enterobacter cancerogenus (Urosević, 1966) Dickey and Zumoff, 1968, is a senior subjective synonym for Enterobacter taylorae Farmer et al., 1985.
Topics: DNA, Bacterial; Enterobacter; Enterobacteriaceae; Nucleic Acid Hybridization; Phenotype; Terminology as Topic
PubMed: 2623364
DOI: 10.1016/0923-2508(89)90067-3 -
Food Science & Nutrition Oct 2023are known to proliferate in cucumber juice, deriving energy from the fermentation of sugars to organic acids and ethanol, and theoretically generating carbon dioxide...
are known to proliferate in cucumber juice, deriving energy from the fermentation of sugars to organic acids and ethanol, and theoretically generating carbon dioxide (CO). We hypothesized that the CO produced by the indigenous in the early stage of cucumber fermentation accumulates in the fermenting fruits causing bloater defect. The ability of seven , indigenous to cucumber, to grow and produce CO in cucumber juice medium (CJM), a sterile model system for cucumber fermentation, was characterized. The induction of bloater defect in cucumber fermentation conducted with pasteurized and acidified fruits was also evaluated. The generation times of the seven in CJM ranged between 0.25 and 8.20 h and resulted in carbon dioxide (CO) production to estimated amounts of 7.22-171.5 mM. and were among the bacteria that produced the most and the least CO in CJM, respectively, at estimated mM concentrations of 171.58 ± 42.96 and 16.85 ± 6.53. Inoculation of and in acidified and pasteurized cucumbers resulted in the production of 138 and 27 mM CO, respectively. Such produced 2% hydrogen in the model cucumber fermentations. A bloater index of 25.4 and 17.4 was calculated from the cucumbers fermented by and , respectively, whereas no defect was observed in the fruits collected from uninoculated control fermentation jars. It is concluded that the metabolic activity of the indigenous to cucumber can produce sufficient CO in cucumber fermentations to induce bloater defect.
PubMed: 37823135
DOI: 10.1002/fsn3.3557 -
Journal of Clinical Microbiology Mar 2005We report a case of osteomyelitis caused by Enterobacter cancerogenus resistant to aminopenicillins in a 56-year-old male who had a motorcycle accident and suffered from... (Review)
Review
We report a case of osteomyelitis caused by Enterobacter cancerogenus resistant to aminopenicillins in a 56-year-old male who had a motorcycle accident and suffered from multiple bone fractures with abundant environmental exposure. E. cancerogenus has rarely been associated with human infections, and its clinical significance remains unclear.
Topics: Accidents, Traffic; Enterobacter; Enterobacteriaceae Infections; Fractures, Bone; Humans; Male; Middle Aged; Multiple Trauma; Osteomyelitis; Wound Infection
PubMed: 15750133
DOI: 10.1128/JCM.43.3.1459-1461.2005 -
Journal of Virology Oct 2012Enterobacter cancerogenus is widely distributed in nature and is generally recovered from environmental or vegetal sources. In some cases, it has also been associated...
Enterobacter cancerogenus is widely distributed in nature and is generally recovered from environmental or vegetal sources. In some cases, it has also been associated with human infections. In this study, the complete genomic sequence of virulent E. cancerogenus bacteriophage Enc34 was determined. The Enc34 genome is 60,364 bp in length and contains 80 open reading frames. To our knowledge, this is the first report of a bacteriophage infecting E. cancerogenus.
Topics: Bacteriophages; Base Sequence; DNA, Viral; Enterobacter; Genome, Viral; Molecular Sequence Data; Sequence Analysis, DNA
PubMed: 22997422
DOI: 10.1128/JVI.01954-12 -
Genome Announcements Feb 2018CR-Eb1 and sp. CR-Ec1 were isolated from the larval gut of , the greater wax moth. Here, we report the completed and annotated genome sequences of insect gut-dwelling...
CR-Eb1 and sp. CR-Ec1 were isolated from the larval gut of , the greater wax moth. Here, we report the completed and annotated genome sequences of insect gut-dwelling bacteria.
PubMed: 29449385
DOI: 10.1128/genomeA.00044-18 -
Frontiers in Microbiology 2020Cucumber fermentations are one of the most important vegetable fermentations in the United States. The fermentation is usually driven by lactic acid bacteria (LAB)...
Cucumber fermentations are one of the most important vegetable fermentations in the United States. The fermentation is usually driven by lactic acid bacteria (LAB) indigenous to fresh cucumbers. But LAB are greatly outnumbered by many Gram-negative bacteria on fresh cucumbers, which may influence the growth of LAB and the incidence of bloater defect (hollow cavities formed inside fermented cucumbers) leading to serious economic loss to the pickle industry. Rapid elimination of Gram-negative bacteria is crucial to the dominance of LAB and the reduction of bloater defect in the fermentation. Various factors can affect the viability of Gram-negative bacteria in cucumber fermentation. Bacteriophages (phages) may be one of such factors. This study explored the abundance, diversity, and functional role of phages infecting Gram-negative bacteria in a commercial cucumber fermentation. Cover brine samples were taken from a commercial fermentation tank over a 30-day period. On day 1 and day 3 of the fermentation, 39 Gram-negative bacteria and 26 independent phages were isolated. Nearly 67% of Gram-negative bacterial isolates were susceptible to phage infection. Phage hosts include , , , , , , , and species. About 88% of the isolated phages infected the members in the family and 58% of phages infected species. Eight phages with unique host ranges were characterized. These phages belong to the , , or family and showed distinct protein profiles and DNA fingerprints. The infectivity of a phage against e was evaluated in cucumber juice as a model system. The phage infection at the multiplicity of infection 1 or 100 resulted in a 5-log reduction in cell concentration within 3 h and rapidly eliminated its host. This study revealed the abundance and variety of phages infecting Gram-negative bacteria, particularly , in the commercial cucumber fermentation, suggesting that phages may play an important role in the elimination of Gram-negative bacteria, thereby facilitating the dominance of LAB and minimizing bloater defect. To our knowledge, this is the first report on the ecology of phages infecting Gram-negative bacteria in commercial cucumber fermentations.
PubMed: 32670232
DOI: 10.3389/fmicb.2020.01306 -
3 Biotech Dec 2021Natural biopolymers have gained remarkable attention for bioremediation particularly in heavy metal removal and oil degradation due to their non-toxic nature and lack of...
Natural biopolymers have gained remarkable attention for bioremediation particularly in heavy metal removal and oil degradation due to their non-toxic nature and lack of secondary pollution. The exopolysaccharides (EPS) produced by the bacteria have become an important class of biopolymers that are employed in bioremediation. The bacteria isolated from the rhizospheric soil have higher metal tolerance and their EPS are effective in biosorption of heavy metals. Here, we report the characterization of an EPS (EPS-RN5) isolated from the root nodule-associated bacteria, strain YU16-RN5 and its heavy metal biosorption abilities. The bacteria isolated from the West coast of India was cultured in yeast extract mannitol (YEM) medium for EPS extraction and to study the production kinetics on a temporal scale. The biochemical composition, rheological properties and thermostability of EPS-RN5 was characterized by standard methods. The biosorption potential of EPS-RN5 against the selected heavy metals was analyzed by employing the inductively coupled plasma atomic emission spectroscopy (ICP-AES) technique. Further, cell culture experiments were used to test the role of EPS-RN5 in reducing the cytotoxicity exerted by the heavy metals in vitro using a human embryonic kidney cell line (HEK 293T). The bacteria showed good growth in YEM media and the maximum EPS yield was 1800 mg/L at 96 h. The molecular weight of EPS-RN5 was 0.7 × 10 Da and it contained 61.5% total sugars and 14.5% proteins. The monosaccharide composition of the EPS included glucose, sorbose and galactose in the ratio 0.25:0.07:1.0. The EPS-RN5 showed high thermal stability with a degradation temperature of 273 °C. Rheological analysis revealed the non-Newtonian behavior, with pseudoplastic characteristics. The EPS-RN5 efficiently absorbed cadmium and other heavy metals such as mercury, strontium, copper, arsenic, and uranium. In vitro studies revealed significant protective effect against the cadmium-induced cytotoxicity in HEK 293T cells. These results indicate the potential applications of EPS-RN5.
PubMed: 34790515
DOI: 10.1007/s13205-021-03034-w