-
Laboratory Animal Research 2020Ethion is an organophosphate used commonly in India despite being banned in many other countries. The present study was designed to study the interaction of ethion and...
Ethion is an organophosphate used commonly in India despite being banned in many other countries. The present study was designed to study the interaction of ethion and lipopolysaccharide (LPS) together on lung after single low dose ethion exposure. Mice ( = 20) were alienated into control and treatment groups ( = 10 each). The treatment group was orally fed ethion (8 mg/kg/animal/day) dissolved in corn oil. The animals ( = 5 each) from both the groups were challenged with 80 μg lipopolysaccharide (LPS) intranasally and the remaining animals ( = 5 each) were administered normal saline solution after 24 h. Ethion along with LPS induced lung inflammation as indicated by increased neutrophils and total leukocyte count (TLC) in broncheoalveolar lavage fluid. Ethion induced histomorphological alterations in lung as shown by increased pulmonary inflammation score in histopathology. Real time PCR analysis showed that ethion followed by LPS resulted significant ( < 0.05) increase in pulmonary Toll-like receptor (TLR)-4 (48.53 fold), interleukin (IL)-1β (7.05 fold) and tumor necrosis factor (TNF)-α (5.74 fold) mRNA expression. LPS co-exposure suggested synergistic effect on TLR4 and TNF-α mRNA expression. Ethion alone or in combination with LPS resulted genotoxicity in blood cells as detected by comet assay. The data suggested single dietary ethion exposure alone or in conjunction with LPS causes lung inflammation and genotoxicity in blood cells.
PubMed: 32742976
DOI: 10.1186/s42826-020-00055-z -
Chemosphere Oct 2017A method for the determination of chlorfenvinphos, ethion and linuron in liver samples by LC-MS/MS is described. Sample treatment was performed by using Sola™...
A method for the determination of chlorfenvinphos, ethion and linuron in liver samples by LC-MS/MS is described. Sample treatment was performed by using Sola™ polymeric reverse phase SPE cartridges after protein precipitation. Gradient elution using 10 mM ammonium formate in methanol (A) and 10 mM ammonium formate in water (B) was used for chromatographic separation of analytes on a Hypersil™ end-capped Gold PFP reverse phase column (100 mm × 2.1 mm, 3 μm). All analytes were quantified without interference, in positive ionization mode using multiple reaction monitoring (MRM) with chlorfenvinphos-d10 as internal standard. The whole procedure was validated according to the FDA guidelines for bioanalytical methods. The calibration curves for chlorfenvinphos, linuron and ethion compounds were linear over the concentration range of 0.005-2 μM (i.e. 0.0018-0.720 μg/mL, 0.0019-0.770 μg/mL and 0.0012-0.500 μg/mL respectively) with coefficients of determination higher than 0.998. A Lower limit of quantification of 0.005 μM was achieved for all analytes, i.e. 5.76, 6.08 and 3.84 μg/kg of liver for chlorfenvinphos, ethion and linuron respectively. Compounds extraction recovery rates ranged from 92.9 to 99.5% with a RSD of 2.3%. Intra- and inter-day accuracies were within 90.9 and 100%, and imprecision varied from 0.8 to 8.2%. Stability tests proved all analytes were stable in liver extracts during instrumental analysis (+12 °C in autosampler tray for 72 h) at the end of three successive freeze-thaw cycles and at -20 °C for up to 9 months. This accurate and robust analytical method is therefore suitable for contamination or metabolism studies.
Topics: Chlorfenvinphos; Chromatography, Liquid; Linuron; Liver; Organothiophosphorus Compounds; Pesticides; Reproducibility of Results; Tandem Mass Spectrometry
PubMed: 28577486
DOI: 10.1016/j.chemosphere.2017.05.157 -
International Journal of Environmental... Nov 2022Accumulation of pesticides has a harmful impact on the environment and human health. The main goal of this work was to develop a method to determine and quantify the...
Accumulation of pesticides has a harmful impact on the environment and human health. The main goal of this work was to develop a method to determine and quantify the residues of thirteen pesticides in edible fish and bivalves such as parati (), seabass ( ssp.), mullet (), clams () and mussel () collected from Sepetiba Bay and Parnaiba River Delta (Brazil) between 2019 and 2020. Matrix solid-phase dispersion (MSPD) was used for extraction and quantification through gas chromatography coupled to tandem mass spectrometry (GC-MS/MS). The method was validated (linearity, accuracy and precision) for fatty fish (), lean fish () and bivalves (). The survey found linear correlation coefficients (r) equal to or greater than 0.9 for almost all analytes. The relative standard deviations (RSD) of five replicates were less than 20% for almost all analytes at different concentrations in lean fish, fatty fish and bivalves. Most analytes showed satisfactory accuracy. Alachlor herbicide was found in samples of seabass, mussels, clams and parati with levels ranging between 0.55 to 420.39 μg kg dw. Ethion was found in parati (maximum 211.22 μg kg dw), mussels (15.1 μg kg dw) and clams (maximum 44.50 μg kg dw). Alachlor was found in clams (maximum 93.1 μg kg dw), and bifenthrin was found in parati (maximum 43.4 μg kg dw) and clams (maximum 42.21 μg kg dw). The validated method was satisfactory for the determination of eleven pesticides in the fatty fish matrix, and thirteen pesticides in the samples of lean fish and bivalves. The presence of alachlor, ethion and bifenthrin stands out.
Topics: Animals; Humans; Tandem Mass Spectrometry; Bays; Agrochemicals; Gas Chromatography-Mass Spectrometry; Bivalvia; Fishes; Pesticides; Smegmamorpha; Solid Phase Extraction
PubMed: 36497865
DOI: 10.3390/ijerph192315790 -
Environmental Analysis, Health and... Dec 2022The aim of the study was to assess the occurrence and distribution of organophosphate compounds residue in soil, surface water, sediment, and banana crops in Araromi...
The aim of the study was to assess the occurrence and distribution of organophosphate compounds residue in soil, surface water, sediment, and banana crops in Araromi farm settlement, Osun State, Nigeria. Organophosphate pesticide residues were determined using a gas chromatography equipped with Flame-Ionization Detection (GC-FID) in 16 soil samples from cocoa and banana farms, 6 water and sediment samples each, and 8 banana samples from 4 farms in the study site. Fourteen organophosphate compounds were detected (acephate, omethoate, dementon-s-methyl, dimethoate, tolcofos-methyl, pirimiphos-methyl, malathion, chlorpyrifos, methidathion, prothiofos, profenofos, ethion, azinphos-methyl and pyrazophos). Tolclofos-methyl, pirimiphos-methyl and prothiofos were detected in all the soil and sediment samples with concentration ranges of 1.9-12.9, 2.25-6.98 and 3.38-9.89 mg/kg respectively in soil and 8.13-9.83, 2.82-25.1 and 3.70-19.5 mg/kg respectively in sediment. Dimethoate, pirimiphos-methyl and prothiofos with concentration ranges, 0.06-0.28, 0.09-0.18 and 0.16-6.11 mg/L respectively were mostly detected in water samples while dimethoate, tolcofos-methyl, malathion, methidathion, prothiofos, ethion and azinphos-methyl compounds were detected in all the banana samples with concentration ranges, 3.40-12.0, 1.82-6.26, 5.73-9.48, 29.7-145, 8.24-20.1, 3.87-9.35 and 3.66-12.2 mg/kg respectively. The organophosphate mean residue concentrations were mostly significantly higher than the Maximum Residue Limits (MRL) at p<0.05. Across the three samples, only pirimiphos-methyl was significantly higher in water samples, omethoate in sediment; acephate, dementon-s-methyl and chlorpyrifos in banana were also not significantly higher at p<0.05. A strong positive significant correlation was observed between the organophosphate compounds in the banana and water samples (R=0.77, p=0.002) at p<0.05. The occurrence of organophosphate compounds in concentrations above MRLs may pose serious environmental and health risks.
PubMed: 36916048
DOI: 10.5620/eaht.2022035 -
Scientific Reports Jun 2023We investigated the plasma levels of pesticides components namely polychlorinated biphenyls (PCBs), dieldrin, dichlorodiphenyldichloroethylene (DDE), ethion, malathion,...
We investigated the plasma levels of pesticides components namely polychlorinated biphenyls (PCBs), dieldrin, dichlorodiphenyldichloroethylene (DDE), ethion, malathion, and chlorpyrifos in recurrent pregnancy loss (RPL) cases, and tested their associations with placental oxidative stress (OS) biomarkers [nitric oxide (NO), thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH), and superoxide dismutase (SOD)] and with placental apoptotic/antiapoptotic indices (Bcl-2 and caspase-3), and evaluated their possible cut-off points to distinguish RPL cases. The study recruited 101 pregnant women divided into; G1 [n = 49, control, normal 1st-trimester pregnancy, normal obstetric history with at least one previous normal live birth], G2 [n = 26, cases with missed abortion (< 3 abortions) before 24 weeks of gestation], and G3 [n = 26, cases with missed abortion (≥ 3 abortions) before 24 weeks of gestation]. The plasma pesticide levels were analyzed by gas chromatography-mass spectrometry. Plasma human chorionic gonadotrophin (HCG), placental OS, Bcl-2, and caspase-3, were analyzed by their corresponding methods and kits. Plasma PCBs, DDE, dieldrin, and ethion levels were significantly higher in RPL cases than in normal pregnancies (p ≤ 0.001). These levels correlated positively with placental OS and apoptosis and negatively with plasma HCG levels. Also, these levels were reliable markers of risk to RPL. Malathion and chlorpyrifos were not detected in any of the study's participants. Pesticides may be risk factors in cases of spontaneous RPL cases. They are associated with an increasing placental OS and placental apoptosis. Specific measures should be taken to decrease maternal exposure to these pollutants' sources, especially in underdeveloped and developing countries.
Topics: Pregnancy; Female; Humans; Placenta; Pesticides; Caspase 3; Polychlorinated Biphenyls; Abortion, Missed; Malathion; Dieldrin; Chlorpyrifos; Case-Control Studies; Abortion, Habitual; Oxidative Stress; Proto-Oncogene Proteins c-bcl-2; Apoptosis
PubMed: 37277462
DOI: 10.1038/s41598-023-36363-2 -
Efficacy of Different Decontamination Processes in Mitigation of Pesticide Residues from Chili Crop.Journal of Food Protection May 2021This study was conducted to evaluate the efficacy of seven decontamination processes in reducing the pesticide mixture load of six insecticides (quinalphos, profenophos,...
ABSTRACT
This study was conducted to evaluate the efficacy of seven decontamination processes in reducing the pesticide mixture load of six insecticides (quinalphos, profenophos, ethion, lambda-cyhalothrin, imidacloprid, and acetamiprid) from chili (Capsicum annuum L.). In the control treatment, the pesticide residues were extracted without following any decontamination procedure. The extraction of the insecticides from chili was initiated after 48 h of pesticide mixture spray and was done using the QuEChERS (quick, easy, cheap, effective, rugged, and safe) method. The quantitative analysis of four insecticides, namely quinalphos, profenophos, ethion, and lambda-cyhalothrin, was done by coupled gas chromatography-electron capture detection and that of imidacloprid and acetamiprid by high-performance liquid chromatography-UV detection. The results depicted reduction of pesticide residues in all the decontamination treatments compared with the control, although it varied for different insecticides. Solutions of 1 and 5% NaCl and 5% CH3COOH served as efficient decontaminants in removal of quinalphos, profenophos, ethion, and lambda-cyhalothrin residues from chili to ca. 90%, whereas for imidacloprid and acetamiprid there was a mild decontamination only (33.33 to 52.44%). The solutions of 5% NaHCO3 and 0.01% KMnO4 were effective only in removing lambda-cyhalothrin residues from the chili crop, but for all other pesticides the decontamination was not much pronounced.
Topics: Chromatography, High Pressure Liquid; Decontamination; Insecticides; Pesticide Residues; Pesticides
PubMed: 33290555
DOI: 10.4315/JFP-20-413 -
Plants (Basel, Switzerland) Nov 2022The indiscriminate use of pesticides in agricultural commodities has become a global health concern. Various household methods are employed to remove pesticide residues...
The indiscriminate use of pesticides in agricultural commodities has become a global health concern. Various household methods are employed to remove pesticide residues from agricultural commodities, e.g., water and ozone. Many ozone-based commercial pesticide removal machines are available in the market for the general public. The current study compares the pesticide removal efficiency of ozone-based washing of fruits and vegetables to simple tap water through commercially available machines and its health risk assessment to different age groups of consumers. The okra and green chili fruits were treated with acetamiprid and ethion as foliar application at the fruiting stage, using the recommended dose (RD) and double to the recommended dose (2RD), respectively. A modified QuEChERS-based pesticide extraction method was verified for its accuracy, precision, linearity, and sensitivity. The treated samples were washed with tap and ozonated water at different intervals, i.e., 3, 8, and 10 min using a commercial food purifier. Washing with ozonized water for 3 min recorded the maximum removal of acetamiprid and ethion from okra and chili fruits. Further, the risk quotient values (RQ) obtained were lower than one at both doses. Thus, washing vegetables with ozonized water for 3 min ensures vegetables are safer for general consumption without any health risk to Indian consumers.
PubMed: 36501242
DOI: 10.3390/plants11233202 -
Chemosphere Aug 2017General population exposure to pesticides mainly occurs via food and water consumption. However, their risk assessment for regulatory purposes does not currently...
General population exposure to pesticides mainly occurs via food and water consumption. However, their risk assessment for regulatory purposes does not currently consider the actual co-exposure to multiple substances. To address this concern, relevant experimental studies are needed to fill the lack of data concerning effects of mixture on human health. For the first time, the present work evaluated on human microsomes and liver cells the combined metabolic effects of, chlorfenvinphos, ethion and linuron, three pesticides usually found in vegetables of the European Union. Concentrations of these substances were measured during combined incubation experiments, thanks to a new analytical methodology previously developed. The collected data allowed for calculation and comparison of the intrinsic hepatic clearance of each pesticide from different combinations. Finally, the results showed clear inhibitory effects, depending on the association of the chemicals at stake. The major metabolic inhibitor observed was chlorfenvinphos. During co-incubation, it was able to decrease the intrinsic clearance of both linuron and ethion. These latter also showed a potential for metabolic inhibition mainly cytochrome P450-mediated in all cases. Here we demonstrated that human detoxification from a pesticide may be severely hampered in case of co-occurrence of other pesticides, as it is the case for drugs interactions, thus increasing the risk of adverse health effects. These results could contribute to improve the current challenging risk assessment of human and animal dietary to environmental chemical mixtures.
Topics: Animals; Chlorfenvinphos; Cytochrome P-450 Enzyme System; Hepatocytes; Humans; Inactivation, Metabolic; Linuron; Liver; Metabolic Clearance Rate; Microsomes, Liver; Organothiophosphorus Compounds; Pesticides
PubMed: 28476006
DOI: 10.1016/j.chemosphere.2017.04.116 -
RSC Advances Jun 2023Organophosphorus (OPs) compounds can endanger human health and the environment by inhibiting the acetylcholinesterase enzyme. But these compounds have been widely used...
Organophosphorus (OPs) compounds can endanger human health and the environment by inhibiting the acetylcholinesterase enzyme. But these compounds have been widely used as pesticides due to their effectiveness against all kinds of pests. In this study, a Needle Trap Device (NTD) packed with mesoporous organo-layered double hydroxide (organo-LDH) material and coupled with gas chromatography-mass spectrometry (GC-MS) was employed for the sampling and analysis of OPs compounds (diazinon, ethion, malathion, parathion, and fenitrothion). In this way, the [magnesium-zinc-aluminum] layered double hydroxide ([Mg-Zn-Al] LDH) modified with sodium dodecyl sulfate (SDS) as a surfactant was prepared and characterized by FT-IR, XRD, BET, and FE-SEM, EDS, and elemental mapping techniques. Then, various parameters such as relative humidity, sampling temperature, desorption time, and desorption temperature were evaluated by the mesoporous organo-LDH:NTD method. The optimal values of these parameters were determined using response surface methodology (RMS) and central composite design (CCD). The optimal temperature and relative humidity values were obtained as 20 °C and 25.0%, respectively. On the other hand, the desorption temperature and time values were in the range of 245.0-254.0 °C and 5 min, respectively. The limit of detection (LOD) and limit of quantification (LOQ) were reported in the range of 0.02-0.05 mg m and 0.09-0.18 mg m, respectively, which shows the high sensitivity of the proposed method compared to the usual methods. The repeatability and reproducibility of the proposed method (by calculating the relative standard deviation) was estimated in the range of 3.8-10.10 which indicates the acceptable precision of the organo-LDH:NTD method. Also, the desorption rate of the stored needles at 25 °C and 4 °C, was determined to be 86.0% and 96.0%, respectively after 6 days. The results of this study proved that the mesoporous organo-LDH:NTD method can be utilized as a fast, simple, environmentally friendly, and effective method for sampling and determining OPs compounds in the air.
PubMed: 37312990
DOI: 10.1039/d3ra01732j