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Genetics May 2022Megasatellites are large tandem repeats found in all fungal genomes but especially abundant in the opportunistic pathogen Candida glabrata. They are encoded in genes...
Megasatellites are large tandem repeats found in all fungal genomes but especially abundant in the opportunistic pathogen Candida glabrata. They are encoded in genes involved in cell-cell interactions, either between yeasts or between yeast and human cells. In the present work, we have been using an iterative genetic system to delete several Candida glabrata megasatellite-containing genes and found that 2 of them were positively involved in adhesion to epithelial cells, whereas 3 genes negatively controlled adhesion. Two of the latter, CAGL0B05061g or CAGL0A04851g, were also negative regulators of yeast-to-yeast adhesion, making them central players in controlling Candida glabrata adherence properties. Using a series of synthetic Saccharomyces cerevisiae strains in which the FLO1 megasatellite was replaced by other tandem repeats of similar length but different sequences, we showed that the capacity of a strain to flocculate in liquid culture was unrelated to its capacity to adhere to epithelial cells or to invade agar. Finally, to understand how megasatellites were initially created and subsequently expanded, an experimental evolution system was set up, in which modified yeast strains containing different megasatellite seeds were grown in bioreactors for more than 200 generations and selected for their ability to sediment at the bottom of the culture tube. Several flocculation-positive mutants were isolated. Functionally relevant mutations included general transcription factors as well as a 230-kbp segmental duplication.
Topics: Candida glabrata; Flocculation; Genome, Fungal; Humans; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins
PubMed: 35274698
DOI: 10.1093/genetics/iyac042 -
Water Research Sep 2021One of the main purposes of drinking water treatment is to reduce turbidity originating from clay particles. Relatively little is known about the removal of other types...
Differences in removal rates of virgin/decayed microplastics, viruses, activated carbon, and kaolin/montmorillonite clay particles by coagulation, flocculation, sedimentation, and rapid sand filtration during water treatment.
One of the main purposes of drinking water treatment is to reduce turbidity originating from clay particles. Relatively little is known about the removal of other types of particles, including conventionally sized powdered activated carbon (PAC) and superfine PAC (SPAC), which are intentionally added during the treatment process; microplastic particles; and viruses. To address this knowledge gap, we conducted a preliminary investigation in full-scale water treatment plants and then studied the removal of these particles during coagulation-flocculation, sedimentation, and rapid sand filtration (CSF) in bench-scale experiments in which these particles were present together. Numbers of all target particles were greatly decreased by coagulation-flocculation and sedimentation (CS). Subsequent rapid sand filtration greatly reduced the concentrations of PAC and SPAC but not the concentrations of viruses, microplastic particles, and clay particles. Overall removal rates by CSF were 4.6 logs for PAC and SPAC, 3.5 logs for viruses, 2.9 logs for microplastics, and 2.8 logs for clay. The differences in removals were not explained by particle sizes or zeta potentials. However, for clays, PAC and SPAC, for which the particle size distributions were wide, smaller particles were less efficiently removed. The ratios of both clay to PAC and clay to SPAC particles increased greatly after rapid sand filtration because removal rates of PAC and SPAC particles were about 2 logs higher than removal rates of clay particles. The trend of greater reduction of PAC concentrations than turbidity was confirmed by measurements made in 14 full-scale water purification plants in which residual concentrations of PAC in treated water were very low, 40-200 particles/mL. Clay particles therefore accounted for most of the turbidity in sand filtrate, even though PAC was employed. The removal rate of microplastic particles was comparable to that of clays. Sufficient turbidity removal would therefore provide comparable removal of microplastics. We investigated the effect of mechanical/photochemical weathering on the removal of microplastics via CSF. Photochemical weathering caused a small increment in the removal rate of microplastics during CS but a small reduction in the removal rate of microplastics during rapid sand filtration; mechanical weathering decreased the removal rate via CS but increased the removal rate via rapid sand filtration. The changes of removal of microplastics might have been caused by changes of their zeta potential.
Topics: Bentonite; Charcoal; Clay; Filtration; Flocculation; Kaolin; Microplastics; Plastics; Sand; Viruses; Water Purification
PubMed: 34418646
DOI: 10.1016/j.watres.2021.117550 -
Polymers Oct 2023The application of polymer flocculants plays a pivotal role in the slurry separation process of shields, and the dispersant used for treating cutter mud cakes can...
The application of polymer flocculants plays a pivotal role in the slurry separation process of shields, and the dispersant used for treating cutter mud cakes can significantly impact the effectiveness of polymer flocculants, potentially leading to reduced efficiency in slurry separation. Experiments were conducted to select appropriate flocculants and investigate the influence of dispersants on flocculant effectiveness, aiming to assess the effect of flocculants and explore the relationships and mechanisms governing their influence. Changes in the patterns of slurry flocculation were revealed in terms of flocculation-driven precipitation and vacuum-filtration effects. The purpose of this article is to provide a reference for the field application of polymer flocculants in the shield field. The conclusions are as follows. Inorganic flocculants containing 0.5% polyaluminum chloride (PAC) exhibit the most effective flocculation, demonstrating strong charge neutralization action. Organic flocculants containing 0.1% cationic polyacrylamides (CPAM) exhibit the most effective flocculation, demonstrating strong bridging and net capture effects. The dispersant sodium hexametaphosphate (SHMP) can significantly weaken the charge-neutralizing action of flocculants and slightly enhance bridging and net capture effects. SHMP can impede the flocculation of slurry with PAC. For CPAM, SHMP can enhance the flocculation of slurry at a low mass fraction (0.1% and 0.3%), while SHMP can significantly hinder flocculation at a high mass fraction (0.5% and 1%). A low mass fraction of SHMP reduced slurry viscosity to 246.3 mPa.s and enhanced vacuum filtration, while a high mass fraction of SHMP increased slurry viscosity to 667.2 mPa.s and hindered vacuum filtration. In conclusion, while dispersants reduce the effectiveness of inorganic flocculants at any mass fraction, a small number of dispersants enhances the performance of organic flocculants; thus, the organic flocculant CPAM is recommended for slurry separation.
PubMed: 37896316
DOI: 10.3390/polym15204073 -
California Medicine Aug 1971This is the transcription of the regular teaching conferences in Infectious Diseases held weekly at Horbor General Hospital, Torrance. The transcription was edited by...
This is the transcription of the regular teaching conferences in Infectious Diseases held weekly at Horbor General Hospital, Torrance. The transcription was edited by Drs. Jerrold Turner, Irwin Ziment and Lucien B. Guze.
Topics: Adult; Female; Flocculation Tests; Hemagglutination Tests; Humans; Middle Aged; Neurosyphilis; Norway; Penicillins; Syphilis; Syphilis Serodiagnosis; Syphilis, Cardiovascular; Syphilis, Cutaneous
PubMed: 5563817
DOI: No ID Found -
International Journal of Environmental... Oct 2022Microplastic (MP) removal from wastewater was investigated using various types and doses of commercial coagulants (PIX, PAX) and flocculants (FPM, PEL, FCT) before...
Microplastic (MP) removal from wastewater was investigated using various types and doses of commercial coagulants (PIX, PAX) and flocculants (FPM, PEL, FCT) before primary clarification in a wastewater treatment plant (WWTP). Dosing with FPM, PIX, and PEL caused small MPs (180-212 µm) to be transferred mainly to the settled sludge (up to 86.4% of MP at a dose of 5 mL FMP/m), while dosing of FCT and PAX caused these MPs to be transferred to the floated sludge (up to 64% MP at a dose of 5 mL PAX/m). The efficiency of MP removal from wastewater was the highest (90%) with 2.5 mL PAX/m; the generated primary sludge had a low MP content and could be safely managed in subsequent stages of sludge treatment. At the highest doses, PIX significantly increased the removal of P-PO (up to 94%) and COD (up to 73%). FPM and FCT resulted in over 40% efficiency of ammonium removal-such disturbance in wastewater composition may negatively affect further biological treatment. Effective removal of MP in the mechanical part of WWTP resulting from coagulation and flocculation enables the safe use of the excess sludge for agricultural purposes.
Topics: Wastewater; Microplastics; Flocculation; Sewage; Plastics; Waste Disposal, Fluid; Water Purification; Ammonium Compounds; Water Pollutants, Chemical
PubMed: 36293592
DOI: 10.3390/ijerph192013013 -
Colloids and Surfaces. B, Biointerfaces Jun 2023Nanoparticles (NPs) can be incorporated into hydrogels to obtain multifunctional hybrid systems to meet the delivery needs of different drugs. However, the stability of...
Nanoparticles (NPs) can be incorporated into hydrogels to obtain multifunctional hybrid systems to meet the delivery needs of different drugs. However, the stability of NPs in hydrogels is rarely revealed. In this article, we tried to explore the underlying mechanism of an interesting phenomenon that poly(lactic-co-glycolic acid) (PLGA) nanoparticles (PNPs) could flocculate and deposit in Pluronic F127 (F127) hydrogels at 4 °C. The results showed that this flocculation was relevant to the type of emulsifier formulated in PNPs, the particle materials and the F127 concentration, but independent of PLGA polymer end groups. Exactly, PNPs containing polyvinyl alcohol (PVA) as the emulsifier flocculated in F127 solution with a concentration above 15 %. The flocculated PNPs possessed increased particle size, decreased zeta potential, reduced hydrophobicity and an obvious coating layer, and these characteristics could be restored almost to the original state after two washes of flocculated PNPs with water. Moreover, the flocculation had no impact on the long-term size stability and drug-loading capacity of PNPs, and F127-treated PNPs showed improved cellular uptake than untreated PNPs. These results provide the evidence that adsorption of high concentrations of F127 on the surface of PNPs/PVA may lead to flocculation, and the flocculation is reversible by simply washing the flocs with water. To the best of our knowledge, this is the first study to scientifically explore the stability of PNPs in F127 hydrogels, providing theoretical and experimental support for the rational design and further development of nanoparticle-hydrogel composite.
Topics: Polylactic Acid-Polyglycolic Acid Copolymer; Poloxamer; Polyglycolic Acid; Lactic Acid; Flocculation; Polyvinyl Alcohol; Hydrogels; Nanoparticles; Particle Size
PubMed: 37156026
DOI: 10.1016/j.colsurfb.2023.113328 -
Marine Pollution Bulletin May 2022Interactions between microplastic (MP) and fine-grained suspended sediment in natural waters are important for the environmental fate of plastic particles. Estuaries are...
Interactions between microplastic (MP) and fine-grained suspended sediment in natural waters are important for the environmental fate of plastic particles. Estuaries are transitional areas between freshwater and open marine systems and are recognized as important accumulation zones for MPs. However, there is a knowledge gap on the processes driving the sedimentation of MPs in estuaries, especially with regard to positively buoyant MPs. Here we show from settling tube experiments that positively buoyant and non-spherical MP HDPE particles in different size-fractions (63-500 μm) and concentrations (1 and 5 mg l) rapidly flocculate and settle with natural fine-grained sediment in natural seawater. Our results demonstrate that flocculation is a key process for the vertical transport of MP in estuaries. The implication is that land-based sources of positively buoyant HDPE MP transported by rivers will likely settle and accumulate in estuarine environments and thereby increase the concentration of MP in the benthic zone.
Topics: Flocculation; Microplastics; Plastics; Polyethylene; Seawater; Water Pollutants, Chemical
PubMed: 35378460
DOI: 10.1016/j.marpolbul.2022.113619 -
International Journal of Environmental... Dec 2020The utilization of metal-based conventional coagulants/flocculants to remove suspended solids from drinking water and wastewater is currently leading to new concerns.... (Review)
Review
The utilization of metal-based conventional coagulants/flocculants to remove suspended solids from drinking water and wastewater is currently leading to new concerns. Alarming issues related to the prolonged effects on human health and further pollution to aquatic environments from the generated nonbiodegradable sludge are becoming trending topics. The utilization of biocoagulants/bioflocculants does not produce chemical residue in the effluent and creates nonharmful, biodegradable sludge. The conventional coagulation-flocculation processes in drinking water and wastewater treatment, including the health and environmental issues related to the utilization of metal-based coagulants/flocculants during the processes, are discussed in this paper. As a counterpoint, the development of biocoagulants/bioflocculants for drinking water and wastewater treatment is intensively reviewed. The characterization, origin, potential sources, and application of this green technology are critically reviewed. This review paper also provides a thorough discussion on the challenges and opportunities regarding the further utilization and application of biocoagulants/bioflocculants in water and wastewater treatment, including the importance of the selection of raw materials, the simplification of extraction processes, the application to different water and wastewater characteristics, the scaling up of this technology to a real industrial scale, and also the potential for sludge recovery by utilizing biocoagulants/bioflocculants in water/wastewater treatment.
Topics: Animals; Bacillaceae; Bacillus; Chlorella; Chromobacterium; Drinking Water; Flocculation; Humans; Paenibacillus; Sewage; Waste Disposal, Fluid; Wastewater; Water Purification
PubMed: 33322826
DOI: 10.3390/ijerph17249312 -
Journal of Environmental Management Apr 2023In the frame of the global phosphorus (P) crisis and ongoing eutrophication issues in the environmental sector, lake sediment can be considered as an alternative P...
In the frame of the global phosphorus (P) crisis and ongoing eutrophication issues in the environmental sector, lake sediment can be considered as an alternative P source after its removal from eutrophic lakes. However, high water contents make sediment dewatering a crucial step towards the efficient reusability of remaining solids. The application of polymeric substances facilitates solid-liquid separation by flocculation of suspended particles. To lower the environmental risk of contamination with toxic, non-biodegradable monomeric residues during and after the application of synthetic polyacrylamide(PAM)-based polymers, switching to natural polymeric substances (biopolymers), e.g., starch- or chitosan-based, is increasingly emphasized. The dewatering performance of four conventional PAM-based polymers was compared to two starch- and one chitosan-based biopolymer. Laboratory experiments were conducted to determine the dewatering rate, floc size and strength, and reject water quality. Biopolymers generally caused the formation of smaller but less shear-sensitive flocs, and lower P levels in the reject water compared to synthetic polymers. Dewatering performance was correlated to the most important functioning influencing polymer-specific properties intrinsic viscosity (polymer extension) and surface charge density (CD). Due to the high CD and low intrinsic viscosity of the biopolymers, electrostatic patch flocculation seems to be the favored flocculation mechanism, while for synthetic polymers bridging seems to be dominating. Solid-liquid separation technologies should be adjusted to the resulting floc size and structure, while surface CD and intrinsic viscosity are important properties for the choice of biopolymer. Overall, biopolymers can function as a more environmentally friendly alternative to synthetic products for lake sediment dewatering accompanied by the potential for P recovery.
Topics: Lakes; Flocculation; Chitosan; Biopolymers; Polymers; Starch; Sewage; Waste Disposal, Fluid
PubMed: 36638721
DOI: 10.1016/j.jenvman.2022.117199 -
Journal of Colloid and Interface Science Jan 2021Microbial extracellular polymeric substances (EPS) produced from wastewater are generally heterodispersed, which is expected to influence their flocculation performances...
HYPOTHESIS
Microbial extracellular polymeric substances (EPS) produced from wastewater are generally heterodispersed, which is expected to influence their flocculation performances and mechanism, particularly in mixed particle systems. The different molecular weight (MW) fractions should contribute to the overall adsorption affinity and flocculation mechanism of EPS in single and dual clay systems.
EXPERIMENTS
EPS harvested from bioreactors were size-fractionated into high, medium and low MW fractions (HMW, MMW, LMW, respectively). The harvested mixed EPS and its fractions were characterised by diverse analytical techniques coupled with optical reflectometry to investigate the role of each EPS fraction in the overall flocculation mechanism of EPS in kaolinite and montmorillonite clay systems.
FINDINGS
In single clay systems, both the harvested mixed EPS and the HMW-EPS fraction showed comparable flocculation performances. However, mixed EPS proved to be more efficient than the HMW-EPS fraction for dual clay flocculation. Site blocking effects were observed in mixed EPS: the LMW and MMW EPS first adsorbed to the surface due to higher diffusivities and faster mass transfer to the interface, while the HMW-EPS were slowly transported but were attached to the surface irreversibly and stronger than the LMW/MMW-EPS. We propose from this, a mixed EPS adsorption mechanism: extended anionic polymer tails in solution, thereby enhancing particle flocculation.
Topics: Adsorption; Biopolymers; Extracellular Polymeric Substance Matrix; Flocculation; Sewage; Wastewater
PubMed: 32814184
DOI: 10.1016/j.jcis.2020.07.146