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Journal of Bacteriology Aug 1964Gordon, M. A. (New York State Department of Health, Albany). The genus Dermatophilus. J. Bacteriol. 88:509-522. 1964.-Seventeen strains of Dermatophilus originating in...
Gordon, M. A. (New York State Department of Health, Albany). The genus Dermatophilus. J. Bacteriol. 88:509-522. 1964.-Seventeen strains of Dermatophilus originating in skin lesions of cattle, sheep, horses, deer, and man were compared as to conditions for growth, colonial characteristics under varying conditions, microscopic morphology, and biochemical reactions. All grew well aerobically at 37 C and were facultatively anaerobic. They were morphologically similar in both gross and microscopic appearance, and most produced motile spores. Stable gray variants often appeared among the orange-yellow "wild-type" colonies. Acid without gas was produced consistently from glucose and fructose, and transitorily from galactose, but was produced from none of eight other carbohydrates except belatedly by some strains from maltose. Almost all strains hydrolyzed casein, most of them digested BCP milk with varying rapidity, and the majority liquefied gelatin, but there was considerable variation in this last property. Differences crossed both host and geographic lines. It is concluded that all isolates can be accommodated in the species D. congolensis Van Saceghem 1915, emend. 1916, 1934, with D. dermatonomus and D. pedis falling into synonymy.
Topics: Actinomycetales; Animals; Carbohydrates; Cattle; Cell Physiological Phenomena; Classification; Culture Media; Electrons; Gram-Positive Cocci; Horses; Humans; Male; Microscopy; Microscopy, Electron; Peptide Hydrolases; Research; Sheep; Sheep, Domestic; Spores, Bacterial
PubMed: 14203370
DOI: 10.1128/jb.88.2.509-522.1964 -
Journal of the American Veterinary... Aug 2019
Topics: Actinobacteria; Animals; Dermatophilus; Ecthyma, Contagious; Female; Goat Diseases; Goats; Gram-Positive Bacterial Infections
PubMed: 31298639
DOI: 10.2460/javma.255.3.313 -
Journal of Bacteriology Apr 1967Shadowed motile elements from actinomycetes were observed with an electron microscope. Included were three strains of Actinoplanes, two of Ampullariella, two of...
Shadowed motile elements from actinomycetes were observed with an electron microscope. Included were three strains of Actinoplanes, two of Ampullariella, two of Dermatophilus, two of Spirillospora, and four of "Nocardia" turbata. In addition, three types of previously undescribed actionmycetes were represented: (i) the C(4) group (four strains) forming substrate mycelium breaking into motile rods; (ii) strain 9-41, forming Microellobosporia-like sporangia with motile spores; and (iii) strain P(2), forming aerial hyphae releasing motile cocci when put in water. All the known chemical cell wall types of actinomycetes except the Nocardia asteroides type and the Actinomyces israeli type were represented in this array of motile actinomycetes. Motile elements were, depending on the genus, cocci, rods (often curved), or pyriform. Flagella were always in tufts (or single), never peritrichous. A relationship seems to exist between the location of the tuft and the cell wall composition. The spores of one strain of Actinoplanes were herniated, thus resembling plasmoptysis forms of bacteria.
Topics: Actinomycetales; Amino Acids; Carbohydrates; Cell Wall; Flagella; Microscopy, Electron; Nocardia; Pimelic Acids
PubMed: 6032515
DOI: 10.1128/jb.93.4.1446-1451.1967 -
Veterinary Sciences Jul 2021Dermatophilosis is a form of dermatitis caused by the bacterium . The disease usually presents as localized purulent dermatitis, crusty hair masses or widespread matting...
Dermatophilosis is a form of dermatitis caused by the bacterium . The disease usually presents as localized purulent dermatitis, crusty hair masses or widespread matting of the hair. This condition is most common in domestic ruminants; but it can also affect other wild animals and humans. Antimicrobial therapy is used in many regions to treat clinical dermatophilosis with varying results. In this study, we aimed to assess the antimicrobial susceptibility of isolates. Fifty-two isolates were obtained from animals showing clinical signs of the disease at farms in St. Kitts. The isolates were then confirmed as by phenotypic tests, PCR and MALDI-TOF Mass Spectrometry. Furthermore, minimum inhibitory concentrations (MIC) of 16 antimicrobial agents were determined, using the broth microdilution method. Although most antimicrobials showed MICs in line with published values, the tetracycline results displayed a clear bimodal distribution over the tested range, with most isolates showing low MICs and 6 isolates much higher values (+/- 100-fold increase). These results indicate the presence of acquired tetracycline resistance in on the island of St. Kitts. Whether the current observation has implications for efficacy of treating the disease must be confirmed in further research.
PubMed: 34357926
DOI: 10.3390/vetsci8070135 -
Veterinary World Nov 2017The study was conducted to isolate and identify (DC) using conventional and molecular diagnostic techniques in scab materials collected from skin infections of sheep...
AIM
The study was conducted to isolate and identify (DC) using conventional and molecular diagnostic techniques in scab materials collected from skin infections of sheep and goats in the Delta region of Tamil Nadu.
MATERIALS AND METHODS
A total of 20 scab samples collected from 18 goats and 2 sheep from Nagapattinam, Thanjavur, and Tiruvarur districts of Tamil Nadu. Smears were made from softened scab materials and stained by either Gram's or Giemsa staining. Isolation was attempted on blood agar plates, and colonies were stained by Gram's staining for morphological identification. Identification was also done by biochemical tests and confirmed by 16S rRNA polymerase chain reaction (PCR), followed by sequencing and phylogenetic analysis of the amplified product.
RESULTS
The peculiar laddering arrangement of coccoid forms in stained smears prepared from scab materials revealed the presence of DC. Isolated colonies from scab materials of sheep and goats on bovine blood agar plate were small, hemolytic, rough, adherent, and bright orange-yellow in color, but some colonies were white to cream color. Gram-staining of cultured organisms revealed Gram-positive branching filaments with various disintegration stages of organisms. 16S rRNA PCR yielded 500 bp amplicon specific for DC. Sequence analysis of a sheep DC isolate showed 99-100% sequence homology with other DC isolates available in NCBI database, and phylogenetic tree showed a close cluster with DC isolates of Congo, Nigeria, and Angola of Africa. Genes for virulence factors such as serine protease and alkaline ceramidase could not be detected by PCR in any of the DC strains isolated of this study.
CONCLUSION
The presence of dermatophilosis in Tamil Nadu was established from this study.
PubMed: 29263591
DOI: 10.14202/vetworld.2017.1314-1318 -
International Journal of Molecular... Jul 2021is a bacterial pathogen mostly of ruminant livestock in the tropics/subtropics and certain temperate climate areas. It causes dermatophilosis, a skin disease that...
is a bacterial pathogen mostly of ruminant livestock in the tropics/subtropics and certain temperate climate areas. It causes dermatophilosis, a skin disease that threatens food security by lowering animal productivity and compromising animal health and welfare. Since it is a prevalent infection in ruminants, dermatophilosis warrants more research. There is limited understanding of its pathogenicity, and as such, there is no registered vaccine against To better understanding the genomics of , the primary aim of this work was to investigate this bacterium using whole-genome sequencing and bioinformatic analysis. is a high GC member of the Actinobacteria and encodes approximately 2527 genes. It has an open pan-genome, contains many potential virulence factors, secondary metabolites and encodes at least 23 housekeeping genes associated with antimicrobial susceptibility mechanisms and some isolates have an acquired antimicrobial resistance gene. Our isolates contain a single CRISPR array Cas type IE with classical 8 Cas genes. Although the isolates originate from the same geographical location there is some genomic diversity among them. In conclusion, we present the first detailed genomic study on , including the first observation of (Z), a tetracycline resistance-conferring gene.
Topics: Actinobacteria; Animals; Anti-Bacterial Agents; Cattle; Cattle Diseases; Computational Biology; Dermatophilus; Genome, Bacterial; Gram-Positive Bacterial Infections; Horse Diseases; Horses; Tetracycline Resistance; Whole Genome Sequencing
PubMed: 34281179
DOI: 10.3390/ijms22137128 -
Infection and Immunity Jul 1971Cutaneous infection in laboratory animals could not be induced with any of several strains of Geodermatophilus. A model for consistent production of streptotrichosis in...
Cutaneous infection in laboratory animals could not be induced with any of several strains of Geodermatophilus. A model for consistent production of streptotrichosis in rabbits, with cultures of Dermatophilus congolensis, is presented.
Topics: Actinomycetales; Actinomycetales Infections; Animals; Arthrodermataceae; Culture Media; Deer; Dermatomycoses; Disease Models, Animal; Female; Guinea Pigs; Male; Mice; Mice, Inbred Strains; Rabbits; Sciuridae; Sheep
PubMed: 5154874
DOI: 10.1128/iai.4.1.29-33.1971 -
Journal of Bacteriology Nov 1963Gordon, Morris A. (Division of Laboratories and Research, New York State Department of Health, Albany), and Mercedes R. Edwards. Micromorphology of Dermatophilus...
Gordon, Morris A. (Division of Laboratories and Research, New York State Department of Health, Albany), and Mercedes R. Edwards. Micromorphology of Dermatophilus congolensis. J. Bacteriol. 86:1101-1115. 1963.-As seen in electron micrographs of thin sections, Dermatophilus congolensis is a holocarpic actinomycete that fragments, after formation of septa in several planes, into Sarcina-like packets and then into individual cocci. Release of coccal forms from the filaments and packets is by dissolution of a capsular matrix, which is a product of degradation of the cell wall. The plasmalemma is a "unit membrane." Regularly occurring plasmalemmosomes ("onion bodies") of uniform structure are apparently related to septum formation. A typical bacterial nucleoid is seen in most sections, and ribosomes are scattered throughout the cytoplasm. Specimens for electron microscopy were prepared by a modification of Kellenberger's method.
Topics: Actinomycetales; Actinomycetales Infections; Animals; Cattle; Cattle Diseases; Electrons; Gram-Positive Cocci; Microscopy; Microscopy, Electron; Research
PubMed: 14080778
DOI: 10.1128/jb.86.5.1101-1115.1963 -
Molecules (Basel, Switzerland) Feb 2022Pitted keratolysis (PK) is a bacterial skin infection mostly affecting the pressure-bearing areas of the soles, causing unpleasant symptoms. Antibiotics are used for...
Pitted keratolysis (PK) is a bacterial skin infection mostly affecting the pressure-bearing areas of the soles, causing unpleasant symptoms. Antibiotics are used for therapy, but the emergence of antiobiotic resistance, makes the application of novel topical therapeutic agents necessary. The antibacterial effects of 12 EOs were compared in the first part of this study against the three known aetiological agents of PK (, and ). The results of the minimal inhibitory concentration, minimal bactericidal concentration and spore-formation inhibition tests revealed that lemongrass was the most effective EO against all three bacterium species and was therefore chosen for further analysis. Seventeen compounds were identified with solid-phase microextraction followed by gas chromatography-mass spectrometry (HS-SPME/GC-MS) analysis while thin-layer chromatography combined with direct bioautography (TLC-BD) was used to detect the presence of antibacterially active compounds. Citral showed a characteristic spot at the Rf value of 0.47, while the HS-SPME/GC-MS analysis of an unknown spot with strong antibacterial activity revealed the presence of α-terpineol, γ-cadinene and calamenene. Of these, α-terpineol was confirmed to possess an antimicrobial effect on all three bacterium species associated with PK. Our study supports the hypothesis that, based on their spectrum, EO-based formulations have potent antibacterial effects against PK and warrant further investigation as topical therapeutics.
Topics: Adult; Anti-Bacterial Agents; Bacteria; Cymbopogon; Humans; Male; Oils, Volatile; Skin Diseases, Bacterial
PubMed: 35209211
DOI: 10.3390/molecules27041423 -
Veterinary Medicine and Science Feb 2020A severe, chronic, locally extensive granulomatous bronchopneumonia was diagnosed on post-mortem and histopathological examination of an adult alpaca. Dermatophilus...
A severe, chronic, locally extensive granulomatous bronchopneumonia was diagnosed on post-mortem and histopathological examination of an adult alpaca. Dermatophilus congolensis organisms were isolated from the lungs and genotypic identification of aerobic culture was confirmed by sequence analysis of the entire 16S rDNA gene. This is the first report of D. congolensis-associated bronchopneumonia in any species.
Topics: Animals; Bronchopneumonia; Camelids, New World; DNA, Bacterial; DNA, Ribosomal; Dermatophilus; Gram-Positive Bacterial Infections; Lung; Male; New South Wales; Sequence Analysis, DNA
PubMed: 31729199
DOI: 10.1002/vms3.213