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Antibiotics (Basel, Switzerland) Oct 2023The immunosuppression conditions and the presence of medical devices in patients favor the infections. However, the features of this aerobic actinomycete have been...
The immunosuppression conditions and the presence of medical devices in patients favor the infections. However, the features of this aerobic actinomycete have been little explored. Strains ( = 164) were characterized with 16S rDNA and A1 genes to define their phylogenetic relationships, and subjected to broth microdilution to profile the antimicrobial susceptibilities of species that caused infections in Spain during the 2005-2021 period. Four out of the eleven identified species were responsible for 86.0% of the infections: (53.0%), (18.3%), (8.5%) and (6.1%). Respiratory tract infections (61.6%) and bacteremia (21.9%) were the most common infections. The A1 gene resolved the inconclusive identification, and two major clonal lineages were observed for and . Species showed a wide antimicrobial susceptibility profile. Cefoxitin resistance varies depending on the species, reaching 94.2% for and 36.0% for . What is noteworthy is the minocycline resistance in (11.5%), the clarithromycin resistance in A1 lineage II (30.0%) and the amoxicillin-clavulanate and cefepime resistance in (21.4% and 42.8%, respectively). and stand out as the prevalent species causing infections in Spain. Resistance against cefoxitin and other antimicrobials should be considered.
PubMed: 37998770
DOI: 10.3390/antibiotics12111568 -
Journal of Clinical Microbiology Apr 2011Gordonia species are aerobic actinomycetes recently recognized as causing human disease, often in the setting of intravascular catheter-related infections. We describe a... (Review)
Review
Gordonia species are aerobic actinomycetes recently recognized as causing human disease, often in the setting of intravascular catheter-related infections. We describe a case of Gordonia bronchialis bacteremia and pleural space infection in the absence of an indwelling intravascular catheter and review the breadth of reported infections with this emerging pathogen.
Topics: Actinomycetales; Actinomycetales Infections; Bacteremia; Blood; DNA, Bacterial; DNA, Ribosomal; Female; Humans; Microscopy; Middle Aged; Molecular Sequence Data; Pleurisy; RNA, Ribosomal, 16S; Radiography, Thoracic; Sequence Analysis, DNA; Tomography, X-Ray Computed
PubMed: 21270217
DOI: 10.1128/JCM.02121-10 -
Pediatric Nephrology (Berlin, Germany) Jan 2022Gordonia species, aerobic, weakly acid-fast, Gram-positive bacilli, are a rare cause of peritonitis in patients undergoing peritoneal dialysis (PD). We report the first...
INTRODUCTION
Gordonia species, aerobic, weakly acid-fast, Gram-positive bacilli, are a rare cause of peritonitis in patients undergoing peritoneal dialysis (PD). We report the first pediatric case of PD-related peritonitis caused by Gordonia bronchialis.
CASE PRESENTATION
A 13-year-old girl with chronic kidney disease (CKD) stage 5D, on continuous cycling PD (CCPD) for 8 years, presented with cloudy PD effluent, with no abdominal discomfort or fever. Intra-peritoneal (IP) loading doses of vancomycin and ceftazidime were started at home after obtaining a PD effluent sample, which showed WBC 2,340 × 10 /L (59% neutrophils) and Gram-positive bacilli. On admission, she was clinically well and afebrile, with no history of methicillin-resistant Staphylococcus aureus (MRSA) infection, so vancomycin was discontinued, and IP ceftazidime and cefazolin were started, following a loading dose of intravenous cefazolin. Gordonia species grew after 5 days of incubation and later identified as Gordonia bronchialis. IP vancomycin was restarted as monotherapy, empirically for a total of 3 weeks therapy. A 2-week course of oral ciprofloxacin was added, based on susceptibility testing. PD catheter replacement was advised due to the risk of recurrence but was refused. A relapse occurred 16 days after discontinuing antibiotics, successfully treated with a 2-week course of IP ceftazidime and vancomycin. The PD catheter was removed and hemodialysis initiated. She received a further 2-week course of oral ciprofloxacin and amoxicillin-clavulanate post PD catheter removal.
CONCLUSIONS
Gordonia bronchialis is an emerging pathogen in PD peritonitis and appears to be associated with a high risk of relapse. PD catheter replacement is strongly suggested.
Topics: Actinobacteria; Adolescent; Female; Humans; Kidney Failure, Chronic; Peritoneal Dialysis; Peritonitis
PubMed: 34633526
DOI: 10.1007/s00467-021-05313-3 -
Cureus Oct 2022species are gram-positive, partially acid-fast bacteria recognized as pathogens associated with medical devices and catheter-related infections in immunocompetent and...
species are gram-positive, partially acid-fast bacteria recognized as pathogens associated with medical devices and catheter-related infections in immunocompetent and immunocompromised hosts. We describe a rare case of bacteremia due to central venous catheter infection in a patient undergoing active chemotherapy for Burkitt lymphoma. We review the diagnosis, treatment, and extent of infections reported throughout medical literature about this rare and emerging pathogen.
PubMed: 36439616
DOI: 10.7759/cureus.30644 -
Innate Immunity Aug 2014The immunomodulatory power of heat-killed Gordonia bronchialis was studied on gut epithelial cells activated with pro-inflammatory stimuli (flagellin, TNF-α or IL-1β)....
The immunomodulatory power of heat-killed Gordonia bronchialis was studied on gut epithelial cells activated with pro-inflammatory stimuli (flagellin, TNF-α or IL-1β). Light emission of luciferase-transfected epithelial cells and mRNA expression of IL-1β, TNF-α, IL-6, CCL20, IL-8 and MCP-1 were measured. NF-κB activation was assessed by immunofluorescence and immunoblotting, and induction of reactive oxygen species (ROS) was evaluated. In vivo inhibitory properties of G. bronchialis were studied with ligated intestinal loop assay and in a mouse model of food allergy. G. bronchialis promoted the down-regulation of the expression of CCL20 and IL-1β on activated epithelial cells in a dose-dependent manner. A concomitant blocking of nuclear p65 translocation with increased production of ROS was found. In vivo experiments confirmed the inhibition of CCL20 expression and the suppression of IgE sensitization and hypersensitivity symptoms in the food allergy mouse model. In conclusion, heat-killed G. bronchialis inhibited the activation of NF-κB pathway in human epithelial cells, and suppressed the expression of CCL20. These results indicate that G. bronchialis may be used to modulate the initial steps of innate immune activation, which further suppress the allergic sensitization. This approach may be exploited as a therapy for intestinal inflammation.
Topics: Animals; Caco-2 Cells; Chemokine CCL20; Cholera Toxin; Dose-Response Relationship, Drug; Down-Regulation; Epithelial Cells; Epithelium; Genes, Reporter; Gordonia Bacterium; Humans; Inflammation Mediators; Male; Mice; Mice, Inbred BALB C; RNA, Messenger; Reactive Oxygen Species; Transcription Factor RelA
PubMed: 24055879
DOI: 10.1177/1753425913503577 -
Emerging Infectious Diseases May 2019Gordonia bronchialis is an aerobic actinomycetes that rarely causes infections in humans. Few reports describe Gordonia spp. causing eye-related infections. We report a...
Gordonia bronchialis is an aerobic actinomycetes that rarely causes infections in humans. Few reports describe Gordonia spp. causing eye-related infections. We report a case of chronic infectious endophthalmitis in Oregon, USA, associated with infection by G. bronchialis.
Topics: Endophthalmitis; Eye Infections, Bacterial; Female; Gordonia Bacterium; Humans; Middle Aged; Oregon
PubMed: 31002051
DOI: 10.3201/eid2505.180340 -
Diagnostics (Basel, Switzerland) Jan 2022In patients with chronic obstructive pulmonary disease, respiratory infections are of various aetiology, predominantly viral and bacterial. However, due to structural...
In patients with chronic obstructive pulmonary disease, respiratory infections are of various aetiology, predominantly viral and bacterial. However, due to structural and immunological changes within the respiratory system, such patients are also prone to mycobacterial and other relatively rare infections. We present the 70-year old male patient with chronic obstructive pulmonary disease (COPD) and coexisting bronchial asthma, diagnosed due to cough with purulent sputum expectoration lasting over three months. The first microbiological investigation of the sputum sample revealed the growth of mycobacteria. The identification test based on protein MPT64 production indicated an organism belonging to NTM ). However, further species identification by genetic testing verified the obtained culture as not belonging to the Mycobacterium genus. Based on observed morphology, the new characterisation identified an aerobic actinomycete, possibly a spp. The isolated strain was recultured on standard microbiological media. The growth of colonies was observed on Columbia blood agar plates and solid Löewenstein-Jensen medium. The Gram and Zhiel-Nielsen stains revealed the presence of Gram-positive acid-fast bacilli. The extraction protocol and identification were performed in two repetitions; the result was , with a confidence value of 99% and 95%, respectively. The gene sequencing method was applied to confirm the species affiliation of this isolate. The resulting sequence was checked against the 16S ribosomal RNA sequences database (Bacteria and Archaea). The ten best results indicated the genus Gordonia (99.04-100%) and 100% similarity of the 16S sequenced region was demonstrated for . The case described indicates that the correct interpretation of microbiological test results requires the use of advanced microbiology diagnosis techniques, including molecular identification of gene sequences. From a clinical point of view, infection or colonization may present a mild course, with no febrile episodes and no significant patient status deterioration and thus, it may remain undiagnosed more often than expected.
PubMed: 35204397
DOI: 10.3390/diagnostics12020307 -
Frontiers in Medicine 2024Corynebacterium, Nocardia, Rhodococcus, Mycobacterium, as well as Gordonia genera belongs to the genus Gordonia, Actinomycetia class. is a nitrate-reducing,...
BACKGROUND
Corynebacterium, Nocardia, Rhodococcus, Mycobacterium, as well as Gordonia genera belongs to the genus Gordonia, Actinomycetia class. is a nitrate-reducing, urease-producing, non-motile, force aerobe with a rod-like figure that is known to arrangement into sessile, cord-like groups. This systematic review aimed to establish whether and what invasive infections in humans were caused by , and to evaluate outcomes of administered antibiotic treatment.
METHODS
We have registered this systematic review in PROSPERO database of systematic reviews and meta-analyses with the number CRD42022369974.
RESULTS
A total of 24 publications were included (22 case reports and two case series) with 28 individual cases. The oldest patients had 92 years, and the youngest patients had 13 years. Clinical signs of infection were present in six patients (21%). All isolates were susceptible to ciprofloxacin, imipenem, and amikacin. Vancomycin was the most frequently used antibiotic with nine cases followed by ciprofloxacin, ceftriaxone, and amoxicillin/clavulanic acid.
CONCLUSION
Although there are no standardized recommendations to date, successful treatment with a favorable outcome has most often been carried out with fluoroquinolones, vancomycin with or without aminoglycosides, as well as carbapenems.
PubMed: 38515988
DOI: 10.3389/fmed.2024.1333663 -
Plastic and Reconstructive Surgery.... Jun 2022The expanding knowledge of the breast microbiome and its constituents necessitates understanding of how it plays into human disease. Consideration of how to identify...
The expanding knowledge of the breast microbiome and its constituents necessitates understanding of how it plays into human disease. Consideration of how to identify novel organisms in breast tissue is a topic of hot debate. We report a case of a 26-year-old woman with repeat incisional break-down and sanguinopurulent drainage who required repeat incision and drainage procedures after bilateral breast reduction. Cultures revealed no growth until 4 months postoperation when matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) revealed , a fastidious, slow-growing organism. To date, there are fewer than 30 reported cases of infections and only one with breast involvement. Our patient required 6 weeks of amoxicillin-clavulanate therapy and frequent follow-up for symptom resolution. This case demonstrates the need for additional microbiologic data in patients with delayed, persistent infections after breast surgery.
PubMed: 35702356
DOI: 10.1097/GOX.0000000000004395 -
International Journal of Infectious... Nov 2022
Topics: Humans; Gordonia Bacterium; Actinobacteria; Pneumonia; Foreign Bodies; RNA, Ribosomal, 16S; DNA, Bacterial; Phylogeny; Sequence Analysis, DNA
PubMed: 36152956
DOI: 10.1016/j.ijid.2022.09.028