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Microbiology Resource Announcements Jan 2022AnarQue and Figliar are bacteriophages identified from the host bacterium Gordonia rubripertincta NRRL B-16540. AnarQue is circularly permuted and has a length of...
AnarQue and Figliar are bacteriophages identified from the host bacterium Gordonia rubripertincta NRRL B-16540. AnarQue is circularly permuted and has a length of 61,822 bp; it is assigned to cluster DR. Figliar has a 3' sticky overhang and a length of 61,147 bp; it is assigned to cluster DJ.
PubMed: 35049345
DOI: 10.1128/mra.01085-21 -
Microbiology Resource Announcements Nov 2022Two lytic phages infecting Gordonia rubripertincta were isolated from irrigated desert soil. Phage LilyPad and PokyPuppy have 64,158-bp and 77,065-bp genomes,...
Two lytic phages infecting Gordonia rubripertincta were isolated from irrigated desert soil. Phage LilyPad and PokyPuppy have 64,158-bp and 77,065-bp genomes, respectively. Based on gene content similarity to phages in the Actinobacteriophage database, LilyPad is assigned to phage subcluster DG1 and PokyPuppy to subcluster CS2.
PubMed: 36314913
DOI: 10.1128/mra.00958-22 -
Microbiology Resource Announcements Oct 2023We report the genomes of two viruses with siphovirus morphology, OtterstedtS21 and Patos, from Albany, New York, using . The genomes of OtterstedtS21 and Patos are ~68...
We report the genomes of two viruses with siphovirus morphology, OtterstedtS21 and Patos, from Albany, New York, using . The genomes of OtterstedtS21 and Patos are ~68 kbp long with 58% GC content. Both phages group with cluster DV based on gene content similarity to phages in the Actinobacteriophage database.
PubMed: 37772859
DOI: 10.1128/MRA.00718-23 -
Biology May 2023The application of strains in biotechnologies of environmental purification as degraders of pollutants of different chemical structures is an interesting research...
The application of strains in biotechnologies of environmental purification as degraders of pollutants of different chemical structures is an interesting research topic. The strain 112 (IEGM112) is capable of utilizing diesel fuel, alkanes, and aromatic compounds. The aim of this work was to study the potential of 112 as a degrader of aromatic and aliphatic compounds and analyze its complete genome in comparison with other known strains. The genome had a total length of 5.28 Mb and contained 4861 genes in total, of which 4799 were coding sequences (CDS). The genome contained 62 RNA genes in total, of which 50 were tRNAs, three were ncRNAs, and nine were rRNAs. The strain bears plasmid elements with a total length of 189,570 nucleotides (plasmid p1517). The strain can utilize 10.79 ± 1.17% of hexadecane and 16.14 ± 0.16% of decane over 3 days of cultivation. In the genome of the strain, we have found metabolic pathways of alkane (cytochrome P450 hydroxylases) and catechol (ortho- and meta-pathways) degradation. These results will help us to further approach the fundamental study of the processes occurring in the strain cells and to enrich our knowledge of the catabolic capabilities of .
PubMed: 37237534
DOI: 10.3390/biology12050721 -
Data in Brief Aug 2020Recent studies have shown that the metal adaptation of Actinobacteria offers a rich source of metal inducible environmentally relevant bio-compounds and molecules. These...
Recent studies have shown that the metal adaptation of Actinobacteria offers a rich source of metal inducible environmentally relevant bio-compounds and molecules. These interact through biosorption towards the unique cell walls or via metal chelating activity of metallophors with trace elements, heavy metals and even with lanthanides to overcome limitations and toxic concentrations. Herein, the purpose is to investigate the adaptation potential of CWB2 in dependence of the rare earths and to determine if we can utilize promising metallophore metal affinities for metal separation from aquatic solutions. For details on data interpretation and applicability of siderophores we refer to the related article entitled "Cultivation dependent formation of siderophores by CWB2" [1]. The respective workflow comprises a metal adaptation method to demonstrate effects on bacterial growth, pH, metallophore production, and metabolic change. All this was evaluated by LC-MS/MS and effects on biosorption of rare earths was verified by ICP-MS. Furthermore, we were able to carry out batch metal adsorption and desorption studies of metallophores entrapped in inorganic polymers of tetramethoxysilane (TMOS) to determine metal chelating capacities and selective enrichment effects from model solutions. The adaptation potential of strain CWB2 at increased erbium and manganese concentrations was verified by increased chelating activity on agar plates, in liquid assays and demonstrated by the successful enrichment of erbium by metallophore-functionalized TMOS-polymers from an aquatic model solution. Furthermore, the number of detected compounds in dependency of rare earths differ in spectral counts and diversity compared to the wild type. Finally, the biosorption of rare earths for the selected adaptation was increased significantly up to 2-fold compared to the wild-type. Overall a holistic approach to metal stress was utilised, integrating a bacterial erbium adaptation, metal chelating, biosorption of lanthanides and immobilization as well as enrichment of metals using metallophore functionalized inorganic TMOS polymers for separation of metals from aquatic model solutions.
PubMed: 32490092
DOI: 10.1016/j.dib.2020.105739 -
Foods (Basel, Switzerland) Nov 2022The characterization and bioactive properties of carotenoid produced by GH-1 originating from Pixian Douban (PXDB), the Chinese traditional condiment, was investigated....
The characterization and bioactive properties of carotenoid produced by GH-1 originating from Pixian Douban (PXDB), the Chinese traditional condiment, was investigated. The produced and purified yellow pigment was characterized by ultraviolet-visible spectroscopy (UV-Vis), Fourier transformed infrared (FTIR), nuclear magnetic resonance (NMR), and high-resolution mass spectrometry (HRMS), and was identified as carotenoid lutein. Additionally, the bioactive activity of lutein from GH-1 was evaluated by measuring the free radical scavenging capacity in vitro and feeding zebrafish lutein through aqueous solution. The results showed that the carotenoid lutein had strong antioxidant capacity and a protective effect on zebrafish eye cells, which could inhibit the apoptosis of eye cells in a concentration dependent manner. The results suggested that carotenoid lutein from GH-1 could be utilized as a potential source of natural antioxidants or functional additives for food/pharmaceutical industries.
PubMed: 36429243
DOI: 10.3390/foods11223649 -
Microbiology Spectrum Sep 2021The glutathione -transferases carried on the plasmid for the styrene-specific degradation pathway in the Gordonia rubripertincta CWB2 were heterologously expressed in...
The glutathione -transferases carried on the plasmid for the styrene-specific degradation pathway in the Gordonia rubripertincta CWB2 were heterologously expressed in Escherichia coli. Both enzymes were purified via affinity chromatography and subjected to activity investigations. StyI and StyJ displayed activity toward the commonly used glutathione -transferase model substrate 1-chloro-2,4-dinitrobenzene (CDNB) with values of 0.0682 ± 0.0074 and 2.0281 ± 0.1301 mM and values of 0.0158 ± 0.0002 and 0.348 ± 0.008 U mg for StyI and StyJ, respectively. The conversion of the natural substrate styrene oxide to the intermediate (1-phenyl-2-hydroxyethyl)glutathione was detected for StyI with 48.3 ± 2.9 U mg. This elucidates one more step in the not yet fully resolved styrene-specific degradation pathway of Gordonia rubripertincta CWB2. A characterization of both purified enzymes adds more insight into the scarce research field of actinobacterial glutathione -transferases. Moreover, a sequence and phylogenetic analysis puts both enzymes into a physiological and evolutionary context. Styrene is a toxic compound that is used at a large scale by industry for plastic production. Bacterial degradation of styrene is a possibility for bioremediation and pollution prevention. Intermediates of styrene derivatives degraded in the styrene-specific pathways are precursors for valuable chemical compounds. The pathway in Gordonia rubripertincta CWB2 has proven to accept a broader substrate range than other bacterial styrene degraders. The enzymes characterized in this study, distinguish CWB2s pathway from other known styrene degradation routes and thus might be the main key for its ability to produce ibuprofen from the respective styrene derivative. A biotechnological utilization of this cascade could lead to efficient and sustainable production of drugs, flavors, and fragrances. Moreover, research on glutathione metabolism in is rare. Here, a characterization of two glutathione -transferases of actinobacterial origin is presented, and the utilization of glutathione in the metabolism of an is proven.
Topics: Actinobacteria; Biotransformation; Epoxy Compounds; Escherichia coli; Glutathione; Glutathione Transferase; Ibuprofen; Phylogeny; Plasmids; Styrenes
PubMed: 34319142
DOI: 10.1128/Spectrum.00474-21 -
Microbiology Resource Announcements Dec 2022Pherobrine and Burley are siphoviruses infecting Gordonia rubripertincta. Pherobrine has a 60,305-bp genome with 89 predicted protein-coding genes, and Burley has a...
Pherobrine and Burley are siphoviruses infecting Gordonia rubripertincta. Pherobrine has a 60,305-bp genome with 89 predicted protein-coding genes, and Burley has a 60,111-bp genome with 90 predicted protein-coding genes. Both phages are assigned to cluster DJ, where they share 78% gene content similarity with each other.
PubMed: 36346246
DOI: 10.1128/mra.01024-22 -
Microbiology Resource Announcements Oct 2023Secretariat and Hydrus are phages grouped into the DJ cluster that were isolated on NRRL B-16540. The phages have 75% nucleotide identity and share 73% gene content....
Secretariat and Hydrus are phages grouped into the DJ cluster that were isolated on NRRL B-16540. The phages have 75% nucleotide identity and share 73% gene content. Secretariat has a genome with 84 predicted genes, while Hydrus has 91 predicted genes and can also infect 3612.
PubMed: 37750725
DOI: 10.1128/MRA.00623-23 -
Microbiology Resource Announcements Oct 2020Jellybones and NHagos are bacteriophages that were identified in the host bacterium NRRL B-16540. Jellybones has a direct terminal repeat and was assigned to the CS2...
Jellybones and NHagos are bacteriophages that were identified in the host bacterium NRRL B-16540. Jellybones has a direct terminal repeat and was assigned to the CS2 subcluster with a length of 77,514 bp. NHagos is circularly permuted and was assigned to the DR cluster with a length of 59,580 bp.
PubMed: 33004457
DOI: 10.1128/MRA.00935-20