Did you mean: gordonia spunti
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Antibiotics (Basel, Switzerland) Oct 2023The immunosuppression conditions and the presence of medical devices in patients favor the infections. However, the features of this aerobic actinomycete have been...
The immunosuppression conditions and the presence of medical devices in patients favor the infections. However, the features of this aerobic actinomycete have been little explored. Strains ( = 164) were characterized with 16S rDNA and A1 genes to define their phylogenetic relationships, and subjected to broth microdilution to profile the antimicrobial susceptibilities of species that caused infections in Spain during the 2005-2021 period. Four out of the eleven identified species were responsible for 86.0% of the infections: (53.0%), (18.3%), (8.5%) and (6.1%). Respiratory tract infections (61.6%) and bacteremia (21.9%) were the most common infections. The A1 gene resolved the inconclusive identification, and two major clonal lineages were observed for and . Species showed a wide antimicrobial susceptibility profile. Cefoxitin resistance varies depending on the species, reaching 94.2% for and 36.0% for . What is noteworthy is the minocycline resistance in (11.5%), the clarithromycin resistance in A1 lineage II (30.0%) and the amoxicillin-clavulanate and cefepime resistance in (21.4% and 42.8%, respectively). and stand out as the prevalent species causing infections in Spain. Resistance against cefoxitin and other antimicrobials should be considered.
PubMed: 37998770
DOI: 10.3390/antibiotics12111568 -
Access Microbiology 2023Although rare, human infections caused by spp. have been reported, especially within the immunocompromised population and those with long-term indwelling devices. We...
INTRODUCTION
Although rare, human infections caused by spp. have been reported, especially within the immunocompromised population and those with long-term indwelling devices. We report a case of spp. bacteraemia in a renal transplant patient and present a literature review on microbiological identification methods of this organism.
CASE PRESENTATION
A 62-year-old female renal transplant recipient admitted to hospital with a 2-month history of dry cough and fevers occurring weekly when receiving electrolyte replacement infusions via a Groshong line. Over 2 weeks, blood cultures repeatedly isolated a Gram-positive bacillus solely in aerobic bottles, and this was initially reported as spp. by the local microbiology laboratory. Chest computed tomography (CT) showed multiple ground-glass lung opacities suggestive of septic pulmonary emboli. As central line-associated bloodstream infection was suspected, empirical antibiotics were initiated and the Groshong line was removed. The Gram-positive bacillus was later confirmed by the reference laboratory as via 16S rRNA sequencing. Vancomycin and ciprofloxacin for a duration of 6 weeks were completed as targeted antimicrobial therapy. After treatment, the patient remained symptom-free with marked improvement on repeat CT chest imaging.
CONCLUSION
This case illustrates the challenges surrounding identification of spp. and other aerobic actinomycetes. 16S rRNA gene sequencing may be a preferred identification method, especially when initial workup of a weakly acid-fast organism fails to make an identification or shows discrepant results using traditional diagnostic modalities.
PubMed: 37424562
DOI: 10.1099/acmi.0.000560.v3 -
IDCases 2020HIV/AIDS has been recognized as a global health issue with significant burden on healthcare services worldwide. Diagnostic and therapeutic challenges include wide range...
HIV/AIDS has been recognized as a global health issue with significant burden on healthcare services worldwide. Diagnostic and therapeutic challenges include wide range of difficult to identify and treat infections. is known to cause multi-system infections in setting of HIV/AIDS. It is often difficult to isolate this organism requiring high suspicion index and special testing techniques. While there is no guidelines-recommended antibacterials regimen for infection, extended combined broad spectrum antibacterials have been successfully used. Our patient in this report is a 50-year-old male with no past history who presented with progressive weakness on the right side of the body and urinary incontinence over the duration of one month. MRI scan of the brain showed bilateral ring-enhancing lesions. was identified from a tissue biopsy using 16S ribosomal RNA sequencing technique. HIV test for antibodies came to be reactive and a CD4 cell count of 7/μL. The patient was treated with combination of antibacterials and had remarkable radiological interval changes and relatively slower yet apparent clinical improvement. Unfortunately, and despite initial recovery, patient has later developed multi-drug resistant hospital acquired pneumonia leading to his death in ICU during course of hospitalization. Treatment of in setting of HIV infection with a combination of antibacterials over extended period appears to be safe and effective. To our knowledge, this is the first report of related multiple brain abscesses as AIDS-presenting illness.
PubMed: 32685375
DOI: 10.1016/j.idcr.2020.e00906 -
BMC Ophthalmology Oct 2017We present a case of post-traumatic endophthalmitis with relatively good prognosis caused by Gordonia sputi, which, to our knowledge is the first case in the literature.
BACKGROUND
We present a case of post-traumatic endophthalmitis with relatively good prognosis caused by Gordonia sputi, which, to our knowledge is the first case in the literature.
CASE PRESENTATION
A 24 year old man, who underwent an intraocular foreign body extraction half a month before presentation in the left eye, was referred to us complaining of blurred vision and slight pain for 5 days. His first presentation showed moderate intracameral and intravitreous purulent inflammation with a best corrected vision of counting fingers. After gram staining of the intravitreous samples revealed a gram-positive bacilli infection, a combination of amikacin and vancomycin was initially injected intravitreously. The left eye kept stable for three days but deteriorated on the 4th day. On the 5th day after presentation conventional culture characterized the bacterium as an Actinomyces sp. while 16S ribosomal RNA gene sequencing confirmed it as Gordonia sputi. Thereby a complete pars plana vitrectomy combined with lensectomy and silicone oil tamponade was performed. During the surgery an intraocular irrigation with penicillin G was adopted, followed by administration of intravenous penicillin G twice one day for a week. A relatively normal fundus with slight intracameral inflammation was observed a week after the operation, and the best corrected vision recovered to 0.15. One year later his vision remained 0.1.
CONCLUSION
Gordonia sputi should be taken into consideration in patients with post-traumatic endophthalmitis especially due to foreign body penetration. Compared to conventional laboratories, molecular methods are recommended for an accurate diagnosis. A comprehensive strategy of antimicrobial agents and vitrectomy may render a satisfactory result.
Topics: Actinomycetales Infections; Anti-Bacterial Agents; Combined Modality Therapy; Endophthalmitis; Endotamponade; Eye Foreign Bodies; Eye Infections, Bacterial; Eye Injuries, Penetrating; Gordonia Bacterium; Humans; Lens, Crystalline; Male; Penicillin G; RNA, Bacterial; RNA, Ribosomal, 16S; Silicone Oils; Vitrectomy; Young Adult
PubMed: 29020920
DOI: 10.1186/s12886-017-0573-5 -
Emerging Infectious Diseases Sep 2009
Topics: Actinomycetales Infections; Aged; Bacteremia; Catheter-Related Infections; France; Gordonia Bacterium; Humans; Immunocompromised Host; Male
PubMed: 19788838
DOI: 10.3201/eid1509.080903 -
Le Infezioni in Medicina Jun 2017We report the first known case of cerebrospinal fluid (CSF) shunt-associated meningitis caused by Gordonia sputi and review published cases of Gordonia CNS infections. (Review)
Review
We report the first known case of cerebrospinal fluid (CSF) shunt-associated meningitis caused by Gordonia sputi and review published cases of Gordonia CNS infections.
Topics: Actinomycetales Infections; Aged, 80 and over; Anti-Bacterial Agents; Bacteremia; Catheter-Related Infections; Device Removal; Diagnostic Errors; Equipment Contamination; Gordonia Bacterium; Humans; Hydrocephalus, Normal Pressure; Linezolid; Male; Meningitis; Postoperative Complications; Urinary Tract Infections; Ventriculoperitoneal Shunt
PubMed: 28603239
DOI: No ID Found -
Biomedical and Environmental Sciences :... Jun 2015Gordonia sputi causes rare bacterial infections resulting from a contaminated indwelling medical device. We report the case of a postoperative plastic expander abscess...
Gordonia sputi causes rare bacterial infections resulting from a contaminated indwelling medical device. We report the case of a postoperative plastic expander abscess in a woman, with G. sputi identification by 16S ribosomal RNA sequencing. This report indicates that Gordonia spp. should be included in the list of organisms causing plastic implant infections.
Topics: Actinomycetales Infections; Adult; Aged; Breast Implants; Equipment Contamination; Female; Gordonia Bacterium; Humans; Infant, Newborn; Male; Mammaplasty; Middle Aged; Plastics
PubMed: 26177910
DOI: 10.3967/bes2015.067 -
Healthcare (Basel, Switzerland) Jul 2023Improvements in medical care have turned severe diseases into chronic conditions, but often their treatment and the use of medical devices are related to specific...
Improvements in medical care have turned severe diseases into chronic conditions, but often their treatment and the use of medical devices are related to specific complications. Here, we present a clinical case of a long-term dialysis patient who was infected with a rare opportunistic infectious agent-. In recent years, the incidence of spp. infections in immunocompromised patients with central venous catheters (CVC) has appeared to rise. The isolation and identification of spp. are challenging and require modern techniques. In addition, the treatment is usually persistent and often results in CVC extraction, which is associated with further risk and costs for the patient. We also studied the alterations in the immune status of the patient caused by long-term renal replacement therapy and persistent hepatitis C virus infection. Antibiotic therapy and immunostimulation with Inosine pranobex lead to successful eradication of the infection without the need for CVC replacement.
PubMed: 37510501
DOI: 10.3390/healthcare11142059 -
Nefrologia : Publicacion Oficial de La... 2016
Topics: Actinomycetales Infections; Aged, 80 and over; Bacteremia; Catheters, Indwelling; Equipment Contamination; Gordonia Bacterium; Humans; Male; Renal Dialysis
PubMed: 27436832
DOI: 10.1016/j.nefro.2016.05.015 -
Pathogens (Basel, Switzerland) Oct 2022Non-tuberculous mycobacteria that cannot be identified at the species level represent a challenge for clinical laboratories, as proper species assignment is key to...
The Re-Identification of Previously Unidentifiable Clinical Non-Tuberculous Mycobacterial Isolates Shows Great Species Diversity and the Presence of Other Acid-Fast Genera.
Non-tuberculous mycobacteria that cannot be identified at the species level represent a challenge for clinical laboratories, as proper species assignment is key to implementing successful treatments or epidemiological studies. We re-identified forty-eight isolates of Ziehl-Neelsen (ZN)-staining-positive "acid-fast bacilli" (AFB), which were isolated in a clinical laboratory and previously identified as species but were unidentifiable at the species level with the hsp65 PCR restriction fragment length polymorphism analysis (PRA). As most isolates also could not be identified confidently via , , or DNA sequencing and a nBLAST search analysis, we employed a phylogenetic method for their identification using the sequences of the rDNA, which resulted in the identification of most AFB and a species diversity not found before in our laboratory. Most were rare species with only a few clinical reports. Moreover, although selected with the ZN staining as AFB, not all isolates belonged to the genus , and we report for the first time in Latin America the isolation of , , and from sputum samples of symptomatic patients. We conclude that ZN staining does not differentiate between the genus and other genera of AFB. Moreover, there is a need for a simple and more accurate tree-based identification method for mycobacterial species. For this purpose, and in development in our lab, is a web-based identification system using a phylogenetic analysis (including all AFB genera) based on rDNA sequences (and in the future multigene datasets) and the closest relatives.
PubMed: 36297216
DOI: 10.3390/pathogens11101159