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Microbiology Resource Announcements Sep 2022Periwinkle is a temperate bacteriophage that was isolated on the host Gordonia terrae 3612. The genome has a length of 55,657 bp and a GC content of 62.9% and contains...
Periwinkle is a temperate bacteriophage that was isolated on the host Gordonia terrae 3612. The genome has a length of 55,657 bp and a GC content of 62.9% and contains 109 protein-coding genes and no tRNA genes. An 8-kb region after the structural protein genes encodes eight membrane proteins, a tyrosine integrase, and an immunity repressor.
PubMed: 36005762
DOI: 10.1128/mra.00696-22 -
Microbiology Resource Announcements Dec 2022Two bacteriophages, GrootJr and NovumRegina, were discovered, sequenced, and annotated. These phages were isolated from distinct soil and water samples, respectively,...
Two bacteriophages, GrootJr and NovumRegina, were discovered, sequenced, and annotated. These phages were isolated from distinct soil and water samples, respectively, on Gordonia terrae 3612. These phages belong to the CR2 subcluster and are similar in genome size and gene content.
PubMed: 36342305
DOI: 10.1128/mra.00703-22 -
Antibiotics (Basel, Switzerland) Oct 2023The immunosuppression conditions and the presence of medical devices in patients favor the infections. However, the features of this aerobic actinomycete have been...
The immunosuppression conditions and the presence of medical devices in patients favor the infections. However, the features of this aerobic actinomycete have been little explored. Strains ( = 164) were characterized with 16S rDNA and A1 genes to define their phylogenetic relationships, and subjected to broth microdilution to profile the antimicrobial susceptibilities of species that caused infections in Spain during the 2005-2021 period. Four out of the eleven identified species were responsible for 86.0% of the infections: (53.0%), (18.3%), (8.5%) and (6.1%). Respiratory tract infections (61.6%) and bacteremia (21.9%) were the most common infections. The A1 gene resolved the inconclusive identification, and two major clonal lineages were observed for and . Species showed a wide antimicrobial susceptibility profile. Cefoxitin resistance varies depending on the species, reaching 94.2% for and 36.0% for . What is noteworthy is the minocycline resistance in (11.5%), the clarithromycin resistance in A1 lineage II (30.0%) and the amoxicillin-clavulanate and cefepime resistance in (21.4% and 42.8%, respectively). and stand out as the prevalent species causing infections in Spain. Resistance against cefoxitin and other antimicrobials should be considered.
PubMed: 37998770
DOI: 10.3390/antibiotics12111568 -
Microbiology Resource Announcements Oct 2022Novel phage Amore2 was isolated from Pittsburgh, Pennsylvania and infects Gordonia terrae 3612. Amore2 was placed into Actinobacteria cluster CS1. Its genome is...
Novel phage Amore2 was isolated from Pittsburgh, Pennsylvania and infects Gordonia terrae 3612. Amore2 was placed into Actinobacteria cluster CS1. Its genome is 73,842 bp with 105 predicted open reading frames and 56.6% GC content. The closest similarity of Amore2 is phage Austin, with a 98% nucleotide identity.
PubMed: 36169313
DOI: 10.1128/mra.00537-22 -
Microbiology Resource Announcements Sep 2022Bacteriophage Finkle is a temperate siphovirus isolated from soil on Gordonia terrae. The 47,895-bp genome has a GC content of 66.6% and encodes 84 protein-coding genes....
Bacteriophage Finkle is a temperate siphovirus isolated from soil on Gordonia terrae. The 47,895-bp genome has a GC content of 66.6% and encodes 84 protein-coding genes. The genome is not closely related to sequences in the Actinobacteriophage database, sharing less than 35% gene content, and was classified as a singleton.
PubMed: 36005761
DOI: 10.1128/mra.00693-22 -
Microbiology Resource Announcements Apr 2023phage APunk was isolated from soil collected in Grand Rapids (MI, USA) using Gordonia terrae 3612. The genome of APunk is 59,154 bp long, has a 67.7% GC content, and...
phage APunk was isolated from soil collected in Grand Rapids (MI, USA) using Gordonia terrae 3612. The genome of APunk is 59,154 bp long, has a 67.7% GC content, and contains 32 protein-coding genes. Based on its gene content similarity to actinobacteriophages, APunk is assigned to phage cluster DE4.
PubMed: 36877085
DOI: 10.1128/mra.01220-22 -
Microbiology Resource Announcements Oct 2022Oregano is a novel cluster CZ4 bacteriophage isolated from the soil using the bacterial host Gordonia terrae. The Oregano genome is 47,575 bp long and encodes two...
Oregano is a novel cluster CZ4 bacteriophage isolated from the soil using the bacterial host Gordonia terrae. The Oregano genome is 47,575 bp long and encodes two tyrosine integrases and a toxin/antitoxin system. It shares an immunity repressor with both and Mycobacterium phages that spans 7 clusters.
PubMed: 36098529
DOI: 10.1128/mra.00679-22 -
Genome Announcements Aug 2016Bacteriophages Phinally and Vivi2 were isolated from soil from Pittsburgh, Pennsylvania, USA, using host Gordonia terrae 3612. The Phinally and Vivi2 genomes are...
Bacteriophages Phinally and Vivi2 were isolated from soil from Pittsburgh, Pennsylvania, USA, using host Gordonia terrae 3612. The Phinally and Vivi2 genomes are 59,265 bp and 59,337 bp, respectively, and share sequence similarity with each other and with GTE6. Fewer than 25% of the 87 to 89 putative genes have predictable functions.
PubMed: 27540050
DOI: 10.1128/genomeA.00599-16 -
Genome Announcements Jun 2016Attis and SoilAssassin are two closely related bacteriophages isolated on Gordonia terrae 3612 from separate soil samples in Pittsburgh, PA. The Attis and SoilAssassin...
Attis and SoilAssassin are two closely related bacteriophages isolated on Gordonia terrae 3612 from separate soil samples in Pittsburgh, PA. The Attis and SoilAssassin genomes are 47,881 bp and 47,880 bp, respectively, and have 74 predicted protein-coding genes, including toxin-antitoxin systems, but no tRNAs.
PubMed: 27365347
DOI: 10.1128/genomeA.00591-16 -
Biodegradation Jun 2020A total of 11 bacterial strains capable of completely degrading 2-butoxyethanol (2-BE) were isolated from forest soil, a biotrickling filter, a bioscrubber, and...
A total of 11 bacterial strains capable of completely degrading 2-butoxyethanol (2-BE) were isolated from forest soil, a biotrickling filter, a bioscrubber, and activated sludge, and identified by 16S rRNA gene sequence analysis. Eight of these strains belong to the genus Pseudomonas; the remaining three strains are Hydrogenophaga pseudoflava BOE3, Gordonia terrae BOE5, and Cupriavidus oxalaticus BOE300. In addition to 2-BE, all isolated strains were able to grow on 2-ethoxyethanol and 2-propoxyethanol, ethanol, n-hexanol, ethyl acetate, 2-butoxyacetic acid (2-BAA), glyoxylic acid, and n-butanol. Apart from the only gram-positive strain isolated, BOE5, none of the strains were able to grow on the nonpolar ethers diethyl ether, di-n-butyl ether, n-butyl vinyl ether, and dibenzyl ether, as well as on 1-butoxy-2-propanol. Strains H. pseudoflava BOE3 and two of the isolated pseudomonads, Pseudomonas putida BOE100 and P. vancouverensis BOE200, were studied in more detail. The maximum growth rates of strains BOE3, BOE100, and BOE200 at 30 °C were 0.204 h at 4 mM, 0.645 h at 5 mM, and 0.395 h at 6 mM 2-BE, respectively. 2-BAA, n-butanol, and butanoic acid were detected as potential metabolites during the degradation of 2-BE. These findings indicate that the degradation of 2-BE by the isolated gram-negative strains proceeds via oxidation to 2-BAA with subsequent cleavage of the ether bond yielding glyoxylate and n-butanol. Since Gordonia terrae BOE5 was the only strain able to degrade nonpolar ethers like diethyl ether, the degradation pathway of 2-BE may be different for this strain.
Topics: Actinobacteria; Biodegradation, Environmental; Comamonadaceae; Cupriavidus; Ethylene Glycols; RNA, Ribosomal, 16S
PubMed: 32356147
DOI: 10.1007/s10532-020-09900-3