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Microorganisms Jun 2022The total diversity of bacterial and fungal communities associated with the phyllosphere (fruits and leaves) of the 'Williams' pear variety was analyzed in two...
The total diversity of bacterial and fungal communities associated with the phyllosphere (fruits and leaves) of the 'Williams' pear variety was analyzed in two phenological stages during fruit development and maturation. The antagonistic potential of autochthonous bacterial and yeast isolates against phytopathogenic fungi was also evaluated. A metabarcoding approach revealed , , , , and as dominant bacterial constituents of the pear phyllosphere, whilst most abundant among the fungal representatives identified were , , , , and . The traditional culturable approach revealed that the genus with , , and was most prevalent. The most frequently cultivated fungal representatives belonged to the genus with six identified species. A broad range of the antagonistic activity was detected for the and yeasts, significantly affecting the growth of many fungal isolates in the range of 53-70%. was the most susceptible fungal isolate. The autochthonous antagonistic yeasts and might be powerful biological control agents of postharvest diseases caused by spp. and common pathogens like , , , and .
PubMed: 35889000
DOI: 10.3390/microorganisms10071282 -
Journal of Oral Microbiology 2017The aim of this study was to characterize the mycobiome associated with oral squamous-cell carcinoma (OSCC). DNA was extracted from 52 tissue biopsies (cases: 25 OSCC;...
The aim of this study was to characterize the mycobiome associated with oral squamous-cell carcinoma (OSCC). DNA was extracted from 52 tissue biopsies (cases: 25 OSCC; controls: 27 intra-oral fibro-epithelial polyps [FEP]) and sequenced for the fungal internal transcribed spacer 2 region using Illumina™ 2 x300bp chemistry. Merged reads were classified to species level using a BLASTN-algorithm with UNITE's named species sequences as reference. Downstream analyses were performed using QIIME™ and linear discriminant analysis effect size. A total of 364 species representing 160 genera and two phyla (Ascomycota and Basidiomycota) were identified, with and making up 48% and 11% of the average mycobiome, respectively. However, only five species and four genera were detected in ≥50% of the samples. The species richness and diversity were significantly lower in OSCC. Genera , , and were overrepresented in OSCC; and were more abundant in FEP. Species-wise, , , and a -like species were enriched in OSCC, while -like species, , and were the most significantly abundant in FEP. In conclusion, a dysbiotic mycobiome dominated by was found in association with OSCC, a finding worth further investigation.
PubMed: 29152157
DOI: 10.1080/20002297.2017.1385369