Authors: Hui Dong, Mengyao Li, Yuqi Yan...

Histamine (HA) is a key biogenic monoamine involved in a wide range of physiological and pathological processes in both the central and peripheral nervous systems. Because the ability to directly measure extracellular HA in real time will provide important insights into the functional role of HA in complex circuits under a variety of conditions, we developed a series of genetically encoded G-protein-coupled receptor-activation-based (GRAB) HA (GRAB) sensors with good photostability, sub-second kinetics, nanomolar affinity, and high specificity. Using these GRAB sensors, we measured electrical-stimulation-evoked HA release in acute brain slices with high spatiotemporal resolution. Moreover, we recorded HA release in the preoptic area of the hypothalamus and prefrontal cortex during the sleep-wake cycle in freely moving mice, finding distinct patterns of HA dynamics between these specific brain regions. Thus, GRAB sensors are robust tools for measuring extracellular HA transmission in both physiological and pathological processes.

Topics: Animals; Mice; Histamine Release; Histamine; Brain; Hypothalamus; Receptors, G-Protein-Coupled

PubMed: 36924772
DOI: 10.1016/j.neuron.2023.02.024

Review

Authors: Shunsuke Takahagi, Akio Tanaka, Michihiro Hide...

Sweat allergy is defined as a type I hypersensitivity against the contents of sweat, and is specifically observed in patients with atopic dermatitis (AD) and cholinergic urticaria (CholU). The allergic reaction is clinically revealed by positive reactions in the intradermal skin test and the basophil histamine release assay by sweat. A major histamine-releasing antigen in sweat, MGL_1304, has been identified. MGL_1304 is produced at a size of 29 kDa by Malassezia (M.) globosa and secreted into sweat after being processed and converted into the mature form of 17 kDa. It induces significant histamine release from basophils of patients with AD and/or CholU with MGL_1304-specific IgE, which is detected in their sera. Patients with AD also show cross-reactivity to MGL_1304-homologs in Malassezia restricta and Malassezia sympodialis, but MGL_1304 does not share cross antigenicity with human intrinsic proteins. Malassezia or its components may penetrate the damaged epidermis of AD lesions and interact with the skin immune system, resulting in the sensitization and reaction to the fungal antigen. As well as the improvement of impaired barrier functions by topical interventions, approaches such as anti-microbial treatment, the induction of tolerance and antibody/substance neutralizing the sweat antigen may be beneficial for the patients with intractable AD or CholU due to sweat allergy. The identification of antigens other than MGL_1304 in sweat should be the scope for future studies, which may lead to better understanding of sweat allergy and therapeutic innovations.

Topics: Antigens, Fungal; Histamine Release; Humans; Hypersensitivity; Malassezia; Sweat

PubMed: 30075993
DOI: 10.1016/j.alit.2018.07.002

Authors: Janet Best, H F Nijhout, Srimal Samaranayake...

BACKGROUND

Histamine (HA), a small molecule that is synthesized from the amino acid histidine, plays an important role in the immune system where it is associated with allergies, inflammation, and T-cell regulation. In the brain, histamine is stored in mast cells and other non-neuronal cells and also acts as a neurotransmitter. The histamine neuron cell bodies are in the tuberomammillary (TM) nucleus of the hypothalamus and these neurons send projections throughout the central nervous system (CNS), in particular to the cerebral cortex, amygdala, basal ganglia, hippocampus, thalamus, retina, and spinal cord. HA neurons make few synapses, but release HA from the cell bodies and from varicosities when the neurons fire. Thus the HA neural system seems to modulate and control the HA concentration in projection regions. It is known that high HA levels in the extracellular space inhibit serotonin release, so HA may play a role in the etiology of depression.

RESULTS

We compare model predictions to classical physiological experiments on HA half-life, the concentration of brain HA after histidine loading, and brain HA after histidine is dramatically increased or decreased in the diet. The model predictions are also consistent with in vivo experiments in which extracellular HA is measured, using Fast Scan Cyclic Voltammetry, in the premammillary nucleus (PM) after a 2 s antidromic stimulation of the TM, both without and in the presence of the H autoreceptor antagonist thioperamide. We show that the model predicts well the temporal behavior of HA in the extracellular space over 30 s in both experiments.

CONCLUSIONS

Our ability to measure in vivo histamine dynamics in the extracellular space after stimulation presents a real opportunity to understand brain function and control. The observed extracellular dynamics depends on synthesis, storage, neuronal firing, release, reuptake, glial cells, and control by autoreceptors, as well as the behavioral state of the animal (for example, depression) or the presence of neuroinflammation. In this complicated situation, the mathematical model will be useful for interpreting data and conducting in silico experiments to understand causal mechanisms. And, better understanding can suggest new therapeutic drug targets.

Topics: Brain; Extracellular Space; Histamine; Histamine H3 Antagonists; Histamine Release; Humans; Models, Theoretical; Receptors, Histamine H3

PubMed: 29228949
DOI: 10.1186/s12976-017-0070-9

Authors: J M Hunter

Topics: Adult; Aged; Female; Histamine Release; Humans; Neuromuscular Blocking Agents

PubMed: 7688493
DOI: 10.1111/j.1365-2044.1993.tb07114.x

Review

Authors: S M MacDonald

There is increasing evidence that human basophils accumulate at sites of chronic inflammation, and, in particular, in allergic asthma. Investigators have, therefore, become very interested in identifying proteins that activate these cells. Recently, the gene encoding a candidate for this function, a novel molecule, the IgE-dependent histamine-releasing factor, was cloned.

Topics: Animals; Biomarkers, Tumor; Histamine Release; Humans; Lymphokines; Tumor Protein, Translationally-Controlled 1

PubMed: 8994855
DOI: 10.1016/s0952-7915(96)80004-5

Review

Authors: C D CALNAN

Topics: Capillaries; Eosinophils; Histamine Release; Humans; Leukocyte Count; Mast Cells; Physiology; Urticaria

PubMed: 14171093
DOI: 10.1136/bmj.2.5410.649

Authors: Kanokvalai Kulthanan, Martin K Church, Eva Maria Grekowitz...

BACKGROUND

Chronic inducible urticaria (CIndU) constitutes a group of nine different CIndUs in which pruritic wheals and/or angioedema occur after exposure to specific and definite triggers. Histamine released from activated and degranulating skin mast cells is held to play a key role in the pathogenesis of CIndU, but evidence to support this has, as of yet, not been reviewed systematically or in detail. We aim to characterize the role and relevance of histamine in CIndU.

METHODS

We systematically searched 3 electronic databases (PubMed, Scopus, and Embase) for studies that reported increased serum or skin histamine concentration (direct evidence) or or histamine release (indirect evidence) following trigger exposure.

RESULTS

An initial total of 3,882 articles was narrowed down to 107 relevant studies of which 52 were in cold urticaria, 19 in cholinergic urticaria, 14 in heat urticaria, 10 in contact urticaria, 7 each in solar urticaria and vibratory angioedema, 4 each in symptomatic dermographism and aquagenic urticaria, and 3 in delayed pressure urticaria. The results of our review support that histamine has a key pathogenic role in the pathogenesis of all CIndUs, but it is not the sole mediator as evidenced by the often poor relationship between the level of histamine and severity of symptoms and the variable clinical efficacy of H-antihistamines.

CONCLUSIONS

Histamine released from skin mast cells is a key driver of the development of signs and symptoms and a promising therapeutic target in CIndU.

Topics: Angioedema; Chronic Urticaria; Histamine; Histamine Release; Humans; Urticaria

PubMed: 35967442
DOI: 10.3389/fimmu.2022.901851

Authors: María Eugenia Riveros, María Inés Forray, Fernando Torrealba...

Appetitive behaviours occur in a state of behavioural and physiological activation that allows the optimal performance of these goal-directed behaviours. Here, we tested the hypothesis that histamine neurons under the command of the infralimbic cortex are important to provide behavioural activation. Extracellular histamine and serotonin were measured by microdialysis of the medial prefrontal cortex in behaving rats in parallel with a picrotoxin microinjection into the infralimbic cortex. The injection aroused the rats behaviourally, increased histamine release and decreased serotonin levels. Inhibition of the infralimbic cortex with muscimol produced the opposite effects on neurotransmitter release. The behavioural activation induced by motivating hungry rats with caged food was paralleled by an immediate histamine release, whereas awakening induced by tapping their microdialysis bowl increased serotonin, but not histamine levels. In conclusion, picrotoxin injection into the infralimbic cortex produces behavioural activation together with histamine release; in a similar manner, induction of an appetitive state produced histamine release, likely related to increased behavioural activation characteristic of an appetitive behaviour.

Topics: Animals; Arousal; Catheters, Indwelling; Cerebral Cortex; Chromatography, High Pressure Liquid; GABA-A Receptor Agonists; GABA-A Receptor Antagonists; Histamine; Histamine Release; Hunger; Male; Microdialysis; Motivation; Muscimol; Neurons; Picrotoxin; Rats, Sprague-Dawley; Receptors, GABA-A; Serotonin

PubMed: 25746330
DOI: 10.1097/FBP.0000000000000129

Authors: D W FAWCETT

Experimental solutions known to affect mast cells or to cause liberation of histamine from the tissue were introduced into the peritoneal cavity of rats. Samples of the peritoneal fluid were withdrawn at intervals afterward and assayed for histamine and the condition of the mast cells was subsequently ascertained by microscopic examination of stained spreads of the mesenteries. Intraperitoneal injection of distilled water caused osmotic disruption of the mast cells and the appearance of an appreciable amount of histamine in the peritoneal fluid. Injection of Tyrode solution alone was not particularly damaging to the mast cells and little or no histamine was released. Injection of Tyrode solution containing compound 48/80 resulted in extensive release of granules from mast cells and the appearance of large amounts of histamine in the fluid. Solution of 48/80 failed however to cause histamine release when injected into rats whose subserosal mast cells had previously been destroyed. A series of increasing doses of compound 48/80 had a graded morphological effect upon mast cells and resulted in a graded increase in the amount of histamine that appeared in the peritoneal fluid. It is unlikely therefore that this compound acts by simply lysing the plasma membrane. It is concluded that mast cells in the rat are extraordinarily rich in histamine which is liberated under conditions which cause mast cells to release their granules. The histamine set free by the potent histamine liberator, compound 48/80, appears to come principally from the tissue mast cells.

Topics: Animals; Cytoplasmic Granules; Histamine; Histamine Release; Mast Cells; Rats; p-Methoxy-N-methylphenethylamine

PubMed: 13286425
DOI: 10.1084/jem.100.2.217

Authors: Yulia Desheva, Andrey Mamontov, Nadezhda Petkova...

Here we evaluate the role of mast cells in infection with influenza A/H5N1 virus in immunized mice. CBA mice were immunized intramuscularly with formalin-inactivated A/Vietnam/1194/2004 (H5N1)NIBRG-14 (H5N1). Serum samples were obtained on days 7, 12, 14, 21 after immunization. At day 14, the mice were infected intranasally with the A/Indonesia/5/2005 (H5N1)IDCDC-RG2 (H5N1) influenza virus with half of the animals receiving a mixture of the antihistamines. 67% of the vaccinated mice were protected from the lethality compared to 43% in the PBS-immunized group. Administration of antihistamines increased survival up to 85%-95%. Immunohistochemical examination using CD117 staining of the lungs demonstrated a larger quantity of activated mast cells after infection of immunized mice compared to mock-immunized mice. This was correlated to increased histamine level in the lungs and blood. Our experimental results suggest the involvement of mast cells and the histamine they produce in the pathogenesis of influenza infection in case of incomplete formation of the immune response to vaccination and mismatch of the vaccine and infection influenza viruses.

Topics: Animals; Cell Degranulation; Chick Embryo; Chlorocebus aethiops; Histamine Release; Influenza A Virus, H5N1 Subtype; Mast Cells; Mice; Orthomyxoviridae Infections; Vero Cells

PubMed: 32777303
DOI: 10.1016/j.lfs.2020.118230