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Emerging Infectious Diseases Jun 2008
Topics: Adolescent; Adult; Child; Child, Preschool; Culture Media; Cystic Fibrosis; DNA Primers; Female; Gram-Negative Bacterial Infections; Humans; Male; Polymerase Chain Reaction; Rhodospirillaceae; Sputum
PubMed: 18507928
DOI: 10.3201/eid1406.071355 -
Respiratory Medicine Case Reports 2021is an uncommon, poorly understood bacterium capable of infecting the respiratory tracts of people with cystic fibrosis. The transmission, clinical relevance and changes...
is an uncommon, poorly understood bacterium capable of infecting the respiratory tracts of people with cystic fibrosis. The transmission, clinical relevance and changes in antimicrobial resistance of over time are unclear due to the low frequency of identification. We report three co-habiting brothers with cystic fibrosis who developed chronic infection and document the clinical and microbiological features of the infections. Clinical evolution after infection varied but was associated with an initial decline in lung function. Familial clustering of this rare pathogen raises the possibility of cross-infection as a potential mechanism of transmission of Inquilinus between CF patients.
PubMed: 34381683
DOI: 10.1016/j.rmcr.2021.101487 -
Veterinary Medicine and Science Jan 2023As the clinical outcome of bite-associated infection is related to the oral commensals, evaluating their composition and antibiotic susceptibility pattern can provide...
BACKGROUND
As the clinical outcome of bite-associated infection is related to the oral commensals, evaluating their composition and antibiotic susceptibility pattern can provide more information for the antibiotic treatment of wound infections and increase the awareness of the multidrug-resistant bacteria in cat oral flora.
AIMS
This study was conducted to identify the various bacterial species in the oral cavity of cats. It aimed to identify the composition of cat oral flora and antibiotic resistant bacterial stains.
MATERIALS AND METHODS
Twenty-two cats were sampled for bacterial evaluation. Matrix-assisted laser desorption/ionization time of flight mass spectrometry was used to provide rapid and reliable detection and identification of the bacterial species. Antibiotic susceptibility tests were performed in the identified isolates to determine the antibiotic susceptibility pattern and to detect the multidrug-resistant bacteria in the cat oral cavities.
RESULTS
A total of 54 isolates were identified, Pasteurella was the genus most commonly isolated from the oral cavity of cats (19/54, 35.19%), followed by Neisseria spp. (8/54, 14.81%) and Staphylococcus spp. (7/54, 12.96%). Uncommon oral flora were isolated from the samples, including Pasteurella canis, Inquilinus limosus and the Enterobacteriaceae family of Escherichia coli, Klebsiella pneumoniae and Serratia marcescens. Fourteen different multidrug-resistant bacteria were detected, including Pasteurella species (4/14), Bacillus species (2/14), Neisseria species (3/14), Escherichia species (1/14) and Staphylococcus species (4/14).
DISCUSSION AND CONCLUSIONS
This study's findings will increase the understanding of the composition of cat oral flora in Hong Kong, which can provide more evidence-based information for the prophylactic treatment of patients with cat bite infections. Moreover, the study identified and detected the antibiotic resistance pattern and multidrug-resistant bacteria in the cat oral cavity, which can help cat owners increase their awareness of maintaining regular oral hygiene for their cats to prevent the spread of pathogens from cats to humans.
Topics: Animals; Cats; Humans; Anti-Bacterial Agents; Hong Kong; Enterobacteriaceae; Drug Resistance, Multiple, Bacterial; Escherichia coli
PubMed: 36520663
DOI: 10.1002/vms3.1040 -
Emerging Infectious Diseases Mar 2023Inquilinus limosus is an environmental bacterium associated with respiratory tract colonization in cystic fibrosis patients. We report a case of I. limosus bacteremia in...
Inquilinus limosus is an environmental bacterium associated with respiratory tract colonization in cystic fibrosis patients. We report a case of I. limosus bacteremia in a patient in France who received a lung transplant and experienced chronic graft dysfunction and SARS-CoV-2 infection. This case suggests I. limosus displays virulence factors associated with invasion.
Topics: Humans; Transplant Recipients; COVID-19; SARS-CoV-2; Bacteremia; Lung
PubMed: 36823767
DOI: 10.3201/eid2903.221564 -
Scientific Reports Jun 2018Selecting appropriate tools providing reliable quantitative measures of individual populations in biofilms is critical as we now recognize their true polymicrobial and...
Selecting appropriate tools providing reliable quantitative measures of individual populations in biofilms is critical as we now recognize their true polymicrobial and heterogeneous nature. Here, plate count, quantitative real-time polymerase chain reaction (q-PCR) and peptide nucleic acid probe-fluorescence in situ hybridization (PNA-FISH) were employed to quantitate cystic fibrosis multispecies biofilms. Growth of Pseudomonas aeruginosa, Inquilinus limosus and Dolosigranulum pigrum was assessed in dual- and triple-species consortia under oxygen and antibiotic stress. Quantification methods, that were previously optimized and validated in planktonic consortia, were not always in agreement when applied in multispecies biofilms. Discrepancies in culture and molecular outcomes were observed, particularly for triple-species consortia and antibiotic-stressed biofilms. Some differences were observed, such as the higher bacterial counts obtained by q-PCR and/or PNA-FISH (≤4 log cells/cm) compared to culture. But the discrepancies between PNA-FISH and q-PCR data (eg D. pigrum limited assessment by q-PCR) demonstrate the effect of biofilm heterogeneity in method's reliability. As the heterogeneity in biofilms is a reflection of a myriad of variables, tailoring an accurate picture of communities´ changes is crucial. This work demonstrates that at least two, but preferentially three, quantification techniques are required to obtain reliable measures and take comprehensive analysis of polymicrobial biofilm-associated infections.
Topics: Anti-Bacterial Agents; Biofilms; Carnobacteriaceae; Cystic Fibrosis; Humans; In Situ Hybridization, Fluorescence; Rhodospirillaceae
PubMed: 29934504
DOI: 10.1038/s41598-018-27497-9 -
Pediatric Pulmonology Jul 2018Patient is a 6-year-old male with CF, MRSA colonization, and pancreatic insufficiency that presented with worsening ppFEV1 and systemic symptoms despite multiple...
PRESENTATION
Patient is a 6-year-old male with CF, MRSA colonization, and pancreatic insufficiency that presented with worsening ppFEV1 and systemic symptoms despite multiple interventions. BAL grew NTM, Stenotrophomonas maltophilia, and Inquilinus limosus, a rare organism found in patients with CF.
COURSE
I. limosus treatment was deferred. Despite treatment of other pathogens, symptoms worsened. I. limosus was targeted with meropenem, amikacin, and ciprofloxacin along with clindamycin for MRSA colonization. Within weeks, symptoms had resolved with ppFEV1 improvement.
DISCUSSION
This case discusses the importance of a rare organism in the CF population. Targeting I. limosus was key to recovery, revealing its potential pathogenicity.
Topics: Amikacin; Anti-Bacterial Agents; Child; Ciprofloxacin; Clindamycin; Cystic Fibrosis; Gram-Negative Bacterial Infections; Humans; Male; Meropenem; Methicillin-Resistant Staphylococcus aureus; Rhodospirillaceae; Stenotrophomonas maltophilia; Virulence
PubMed: 29790674
DOI: 10.1002/ppul.24043 -
Journal of Clinical Microbiology Jun 2002Using a polyphasic approach (including cellular protein and fatty acid analysis, biochemical characterization, 16S ribosomal DNA sequencing, and DNA-DNA hybridizations),...
Using a polyphasic approach (including cellular protein and fatty acid analysis, biochemical characterization, 16S ribosomal DNA sequencing, and DNA-DNA hybridizations), we characterized 51 bacterial isolates recovered from respiratory secretions of cystic fibrosis (CF) patients. Our analyses showed that 24 isolates belong to taxa that have so far not (or only rarely) been reported from CF patients. These taxa include Acinetobacter sp., Bordetella hinzii, Burkholderia fungorum, Comamonas testosteroni, Chryseobacterium sp., Herbaspirillum sp., Moraxella osloensis, Pandoraea genomospecies 4, Ralstonia gilardii, Ralstonia mannitolilytica, Rhizobium radiobacter, and Xanthomonas sp. In addition, one isolate most likely represents a novel Ralstonia species, whereas nine isolates belong to novel taxa within the alpha-PROTEOBACTERIA: Eight of these latter isolates are classified into the novel genus Inquilinus gen. nov. as Inquilinus limosus gen. nov., sp. nov., or as Inquilinus sp. The remaining 17 isolates are characterized as members of the family ENTEROBACTERIACEAE: The recovery of these species suggests that the CF lung is an ecological niche capable of supporting the growth of a wide variety of bacteria rarely seen in clinical samples. Elucidation of the factors that account for the association between these unusual species and the respiratory tract of CF patients may provide important insights into the pathophysiology of CF infection. Because accurate identification of these organisms in the clinical microbiology laboratory may be problematic, the present study highlights the utility of reference laboratories capable of identifying unusual species recovered from CF sputum.
Topics: Alphaproteobacteria; Bacterial Proteins; Bacterial Typing Techniques; Cystic Fibrosis; DNA, Ribosomal; Enterobacteriaceae; Fatty Acids; Humans; Molecular Sequence Data; Nucleic Acid Hybridization; Phylogeny; RNA, Ribosomal, 16S; Respiratory System; Sequence Analysis, DNA; Sputum
PubMed: 12037065
DOI: 10.1128/JCM.40.6.2062-2069.2002 -
Brazilian Journal of Microbiology :... 2015The bacterium, Inquilinus limosus, with its remarkable antimicrobial multiresistant profile, has increasingly been isolated in cystic fibrosis patients. We report draft...
The bacterium, Inquilinus limosus, with its remarkable antimicrobial multiresistant profile, has increasingly been isolated in cystic fibrosis patients. We report draft genome sequence of a strain MP06, which is of considerable interest in elucidating the associated mechanisms of antibiotic resistance in this bacterium and for an insight about its persistence in airways of these patients.
Topics: Anti-Bacterial Agents; Base Sequence; Drug Resistance, Multiple, Bacterial; Genome, Bacterial; Gram-Negative Bacterial Infections; Humans; Molecular Sequence Data; Rhodospirillaceae
PubMed: 26691451
DOI: 10.1590/s1517-838246420150474 -
The New Microbiologica Oct 2014Cystic fibrosis is a genetic disorder associated with a polymicrobial lung infection where classical pathogens and newly identified bacteria may interact. Inquilinus...
Cystic fibrosis is a genetic disorder associated with a polymicrobial lung infection where classical pathogens and newly identified bacteria may interact. Inquilinus limosus is an a-proteobacterium recently isolated in the airways of cystic fibrosis patient. We report the first case in Italy of I.limosus isolation from the sputum sample of a cystic fibrosis patient. The patient is a 20-years-old man with cystic fibrosis, regularly attending the Regional Care Center for Cystic Fibrosis at the Federico II University Hospital of Naples. Microbiological culture methods detected a mu- coid gram negative bacillus in the patient's sputum sample. The isolate exhibited a distinct antimicrobial suscep- tibility profile with a high MIC for several drugs. The MALDI-TOF mass spectrometry analysis indicated the bac- terium isolated as I. limosus, confirmed by 16s rDNA sequence analysis. The described clinical case demonstrates how the bacterial biodiversity in the airways of cystic fibrosis patients is still underestimated. Cystic fibrosis lung represents an ecological niche suitable for growth of a wide variety of unusual bacteria not commonly associated with human diseases, such as I. limosus. Therefore further studies are needed to evaluate the epidemiology and clinical implications of I. limosus in the physiopathology of cystic fibrosis lung infection.
Topics: Alphaproteobacteria; Anti-Bacterial Agents; Cystic Fibrosis; Humans; Italy; Male; Young Adult
PubMed: 25387296
DOI: No ID Found -
Biotechnology Reports (Amsterdam,... Mar 2021Two synthetic bacterial consortia (SC) composed of bacterial strains sp. (AM), (B), sp. (Bc-h and T), sp. (Bk) and (Inq) isolated from a natural phenanthrene...
Two synthetic bacterial consortia (SC) composed of bacterial strains sp. (AM), (B), sp. (Bc-h and T), sp. (Bk) and (Inq) isolated from a natural phenanthrene (PHN)-degrading consortium (CON) were developed and evaluated as an alternative approach to PHN biodegradation in bioremediation processes. A metabolic network showing the potential role of strains was reconstructed by study of the six genomes and classification of dioxygenase enzymes using RHObase and AromaDeg databases. Network analysis suggested that AM and Bk were responsible for PHN initial attack, while Inq, B, T and Bc-h would degrade PHN metabolites. The predicted roles were further confirmed by physiological, RT-qPCR and metaproteomic assays. SC-1 with AM as the sole PHN degrader was the most efficient. The ecological roles inferred in this study can be applied to optimize the design of bacterial consortia and tackle the biodegradation of complex environmental pollutants.
PubMed: 33489789
DOI: 10.1016/j.btre.2021.e00588