Review

Authors: M G Diskin

In cattle artificial insemination plays not only a vital role in the successful establishment of pregnancy, which is a prerequisite for initiation of the subsequent lactation, but also in accelerating genetic improvement and facilitating the distribution of semen from genetically elite sires. The latter has been greatly facilitated by the ability to successfully cryopreserve semen. The objective of an insemination is to ensure that there is an adequate reservoir of competent, capacitated, motile sperm in the caudal region of the oviductal isthmus, the site of the main sperm reservoir in the cow, at the time of ovulation to ensure fertilisation. Handling of semen, particularly the 0.25 ml straw, is critically important. Thawed semen needs to be protected from cold and heat shocks and inseminated within 6 to 8 min of thawing. Uterine horn insemination give a modest improvement in conceptions rates particularly in situations where conception rates are low following uterine body inseminations. Most of the studies that evaluated heterospermic insemination were conducted on fresh semen only, and many lacked adequate replication. Consequently, it is difficult to deduce if there are real benefits from using heterospermic semen. While the interval from oestrous onset to time of ovulation would appear to be similar for cows and heifers at about 28 h there is huge variation (standard deviations of 5 to 6 h) around this average. While best conception rates are achieved when cows are inseminated from mid oestrus to a few hours after the onset of oestrus, this is difficult to achieve in practice. There is emerging evidence that having one insemination time, when all cows requiring insemination in the herd on that day are inseminated, does not compromise fertility provided insemination technique is good and the semen used is of high fertility.

Topics: Animals; Cattle; Female; Fertility; Insemination, Artificial; Male; Pregnancy; Semen; Semen Preservation; Specimen Handling; Spermatozoa

PubMed: 29717688
DOI: 10.1017/S1751731118000952

Authors: P R Garner

Topics: Female; Humans; Insemination, Artificial; Insemination, Artificial, Heterologous; Insemination, Artificial, Homologous; Male; Patient Selection; Pregnancy; Spermatozoa; Spouses

PubMed: 761122
DOI: No ID Found

Authors: S J BEHRMAN

Topics: Humans; Insemination, Artificial

PubMed: 13653160
DOI: 10.1016/s0015-0282(16)33424-0

Review

Authors: J M G Souza-Fabjan, M E F Oliveira, M P P Guimarães...

Artificial insemination (AI) and in vivo embryo production (or multiple ovulation and embryo transfer, MOET) programs are both instrumental in accelerating the propagation of genetically and economically superior goats and sheep. The aim of this review was to present the current gestalt of non-surgical AI and embryo recovery (NSER) procedures in small ruminants. Small body size, precluding rectal palpation, and highly limited penetrability of the uterine cervix in ewes are the major reasons for the scarce use of non-surgical assisted reproduction techniques in this species. As a result, AI and embryo recovery techniques in sheep mainly involve laparoscopy or laparotomy (LAP). In does, however, the Embrapa method of AI allows for successful intrauterine deposition of semen, resulting in pregnancy rates from 50 to 80% under field conditions (>3 000 goats inseminated) when frozen-thawed semen is used. After the administration of prostaglandin F (PGF), non-surgical (transcervical) embryo recovery is also feasible in goats, with the cervical penetration rate approaching 100%. There is a paucity of information on the efficacy of non-surgical AI using frozen semen in sheep, but the results are satisfactory with fresh, cooled, or chilled ram semen. An application of the NSER technique in ewes has greatly improved over the last decade, and cervical penetration rates of ∼90% can be achieved when a hormonal cervical dilation protocol using PGF, oxytocin, and/or estradiol ester (e.g., estradiol benzoate) is applied. In some genotypes of sheep, sufficient cervical dilation can be induced without estradiol ester included in the protocol. Several studies indicated that recovery of transferable quality ovine embryos using NSER is comparable to that employing a ventral midline laparotomy, and NSER is evidently a method of choice when animal welfare is concerned. Considering both the number of retrievable embryos and animal well-being, the NSER is a viable alternative for surgical procedures. With further developments, it has the makings of a primary, if not exclusive, embryo recovery technique in small ruminants worldwide.

Topics: Pregnancy; Sheep; Animals; Male; Female; Insemination, Artificial; Semen Preservation; Estradiol; Ruminants; Goats

PubMed: 37567658
DOI: 10.1016/j.animal.2023.100787

Review

Authors: J P Brillard

Recent observations in turkey and chicken hens show that sperm storage in both species is a highly inefficient process. After artificial insemination (AI), less than 1% of spermatozoa inseminated are selected for transport to and enter the sperm storage tubules (SST). It has been shown that the sperm selection process is orchestrated within the vagina and not at the level of the SST. At least two mechanisms are involved in the selection of spermatozoa fit for sperm storage, one being mechanical (motility) and the other biochemical in nature (sperm-vaginal mucosa interactions). Furthermore, it was also observed that the sperm storage efficiency in the chicken is dependent upon the logarithm of the number of spermatozoa inseminated. From a practical standpoint, inseminations performed frequently with a moderate number of spermatozoa should be more efficient than inseminations performed with higher doses at longer intervals. Maximal filling of the SST of hens in egg production requires only 1 day for the chicken and 2 days for the turkey. By contrast, the release of sperm from the SST is about seven times faster in the chicken than the turkey hen. The efficiency of oviducal sperm storage is related to a number of factors including age of the hen, stage of the ovulatory cycle when inseminated, and, in the turkey, if the hen was inseminated before or after the onset of egg production. Two different categories should be considered among factors that affect sperm survival in vivo. 1) Factors affecting sperm storage.(ABSTRACT TRUNCATED AT 250 WORDS)

Topics: Animals; Copulation; Female; Fertilization; Insemination, Artificial; Male; Poultry; Sperm Transport; Spermatozoa

PubMed: 8502613
DOI: 10.3382/ps.0720923

Review

Authors: J Roca, I Parrilla, A Bolarin...

AI is commercially applied worldwide to breed pigs, yielding fertility outcomes similar to those of natural mating. However, it is not fully efficient, as only liquid-stored semen is used, with a single boar inseminating about 2000 sows yearly. The use of liquid semen, moreover, constrains international trade and slows genetic improvement. Research efforts, reviewed hereby, are underway to reverse this inefficient scenario. Special attention is paid to studies intended to decrease the number of sperm used per pregnant sow, facilitating the practical use of sexed frozen-thawed semen in swine commercial insemination programs.

Topics: Animals; Female; Insemination, Artificial; Male; Pregnancy; Semen Preservation; Spermatozoa; Swine

PubMed: 26723133
DOI: 10.1016/j.theriogenology.2015.11.026

Authors:

Topics: Abortion, Spontaneous; Female; Humans; Infertility; Insemination, Artificial; Insemination, Artificial, Homologous; Male; Pregnancy

PubMed: 567076
DOI: No ID Found

Authors: Yifan Chen, Sunantha Kosonsiriluk, Lillian X Ehresmann...

Weekly artificial insemination (AI) is a common practice on commercial turkey breeder farms. The aim of this study was to determine changes in stress as well as oviduct and systemic immunity in response to weekly artificial inseminations through the laying cycle of turkey hens. Hens were divided into sham (extender only) and semen (extender + sperm) treatments. Blood, uterovaginal junction (UVJ), vagina, and spleen were collected at start of lay, peak lay, and end of lay (n = 8-12 /group for blood and 5 for tissues). The heterophil to lymphocyte ratio (H:L) was significantly higher in the semen-inseminated hens compared with the sham-inseminated hens at peak lay (n = 0.05). Immunohistochemistry revealed a higher number of CD3+ T cells in sperm storage tubules (SSTs) and UVJ submucosa at the end of lay compared with start and peak of lay across insemination treatments (n = 0.07 and 0.01, respectively). Within the end-of-lay group, semen-inseminated hens showed a higher number of CD3+ T cells in SSTs and UVJ submucosa (n = 0.04 and 0.1, respectively). The number of IgM+ B cells was significantly higher at start of lay compared with end of lay in SSTs and spleen (n = 0.01 and 0.0001, respectively) regardless of insemination treatment. In the vaginal submucosa, the number of IgM+ B cells was significantly higher in the semen group compared with the sham group at peak lay (n = 0.04). The number of IgA+ and IgY+ B cells were higher in the UVJ submucosa at the end of lay compared with start of lay, regardless of insemination treatment (n = 0.0001 and 0.03 respectively). In summary, the localization and number of adaptive immune cells change in response to the presence of sperm and laying cycle and depend on factors including immune cell type and tissue compartment. This suggests that the adaptive immune system of the oviduct plays an important role in responding to sperm based on the stage of the laying cycle. Modulating this immune response could improve reproductive performance.

Topics: Animals; Female; Turkeys; Insemination, Artificial; Adaptive Immunity; Semen; Genitalia, Female; Male

PubMed: 39527869
DOI: 10.1016/j.psj.2024.104448

Authors: S Pellegrini, G A Orso, R H Marin...

Japanese quails in wild life live in small groups with females being even solitary during the laying period. Although it is a poultry species widely used for egg production, information regarding laying behavior motivations or influencing variables is scarce. Our study focuses on evaluating along 7 d the quail laying behavior in a novel environmental set up. This set up allows the female to choose between remaining separated from a conspecific in one side of the apparatus or to voluntarily enter their space (box-mate side) and interact with it. We evaluated whether the female insemination status prior to enter the environmental set up, and the presence of a female or a male partner in the box-mate side can influence their laying and social behavior. Thus, 4 experimental groups were established. Females spent a higher (P < 0.05) percentage of time in the box-mate side than in their separated sector in all groups. In 3 of the 4 experimental groups (non-inseminated females interacting with a female or a male box-mate, and inseminated females interacting with a male box-mate) females also laid a greater percentage (≥65%, P < 0.05, in all cases) of eggs in the box-mate sector than in their separated sector. However, the group of inseminated females that interacted with a female box-mate shifted their egg distribution and laid equally between both sides of the apparatus. Aggressive social interactions were reduced (P < 0.05) throughout the testing days but this was depending upon the female insemination status and the sex of their box-mate. Findings suggest that females can change their laying side choice when they are inseminated but depending on the sex identity of their box-mate partners. Thus, providing quail female breeders with the option of laying their eggs in separated enclosures from conspecifics could be key to favor their well-being.

Topics: Female; Male; Animals; Quail; Oviposition; Chickens; Ovum; Coturnix; Social Behavior; Insemination; Social Environment

PubMed: 36476681
DOI: 10.1016/j.psj.2022.102328

Authors: Samir Al-Bulushi, Bodhaganahalli M Manjunatha, Roslyn Bathgate...

This study was conducted to evaluate various factors affecting fertility following insemination of dromedary camels. In experiment 1, camels were either bred by natural mating (NM) or inseminated in the body of uterus with whole, split (50:50) or 1 mL of undiluted ejaculate. In experiment 2, camels were inseminated with fresh diluted semen either in the body of the uterus or tip of the uterine horn and at either the time of ovulation induction (0 h), 24 or 30 h later. In experiment 3, camels were inseminated at the tip of the uterine horn with different doses of fresh diluted semen (75, 150 or 300 x 106 motile spermatozoa) or with 150 x 106 motile spermatozoa diluted with different extenders (Green buffer, Optixcell or Triladyl). In experiment 4, camels were inseminated in the tip of the uterine horn with diluted (Triladyl or Optixcell) liquid-stored semen or diluted (Triladyl) frozen-thawed semen consisting of either 300 or 500 x 106 motile spermatozoa. The pregnancy rate in camels bred by NM was similar to camels inseminated with whole undiluted ejaculates whereas insemination with 1 mL undiluted ejaculate resulted in lower pregnancy compared to whole and split undiluted ejaculates (P < 0.05). Deposition of semen in the uterine body resulted in lower pregnancy rates compared to deposition in the tip of the horn (35.3% versus 72.2%, P < 0.05) but insemination at the time of ovulation induction and 24 h later resulted in higher pregnancy rate to camels inseminated at 30 h after induction (68.4 and 70.0% versus 23.5%; P < 0.05). Artificial insemination with 75 x 106 motile spermatozoa resulted in lower pregnancy rates compared to 150 and 300 x 106 motile spermatozoa doses (40.9% versus 65.2 and 70.0%, respectively) and pregnancy rate was not affected by extenders. Insemination of chilled motile spermatozoa stored in either Triladyl or Optixcell resulted in similar pregnancy rates, regardless of insemination dose, although an upward trend with increasing sperm number was apparent (Triladyl; 11.1% versus 21.1% and Optixcell; 5.9% versus 12.5%, for 300 x 106 and 500 x 106 groups, respectively; P > 0.05). No pregnancies were obtained with frozen thawed semen. In conclusion, this study demonstrated that the success of camel AI is highly dependent on sperm dose, location of semen deposition, timing of insemination and semen type. Further studies are required to determine the reason for the compromised fertility of preserved semen despite apparent high in vitro quality.

Topics: Animals; Camelus; Cryopreservation; Female; Insemination, Artificial; Male; Ovulation; Pregnancy; Pregnancy Rate; Semen; Semen Preservation; Sexual Behavior, Animal; Spermatozoa

PubMed: 31697770
DOI: 10.1371/journal.pone.0224992