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Viruses Mar 2022Surface plasmon resonance and biolayer interferometry are two common real-time and label-free assays that quantify binding events by providing kinetic parameters. There... (Review)
Review
Surface plasmon resonance and biolayer interferometry are two common real-time and label-free assays that quantify binding events by providing kinetic parameters. There is increased interest in using these techniques to characterize whole virus-ligand interactions, as the methods allow for more accurate characterization than that of a viral subunit-ligand interaction. This review aims to summarize and evaluate the uses of these technologies specifically in virus-ligand and virus-like particle-ligand binding cases to guide the field towards studies that apply these robust methods for whole virus-based studies.
Topics: Biological Assay; Biosensing Techniques; Interferometry; Kinetics; Ligands; Surface Plasmon Resonance
PubMed: 35458446
DOI: 10.3390/v14040717 -
Biosensors Dec 2022Biosensing and bioimaging are essential in understanding biological and pathological processes in a living system, for example, in detecting and understanding certain... (Review)
Review
Biosensing and bioimaging are essential in understanding biological and pathological processes in a living system, for example, in detecting and understanding certain diseases. Optical fiber has made remarkable contributions to the biosensing and bioimaging areas due to its unique advantages of compact size, immunity to electromagnetic interference, biocompatibility, fast response, etc. This review paper will present an overview of seven common types of optical fiber biosensors and optical fiber-based ultrasound detection in photoacoustic imaging (PAI) and the applications of these technologies in biosensing and bioimaging areas. Of course, there are many types of optical fiber biosensors. Still, this paper will review the most common ones: optical fiber grating, surface plasmon resonance, Sagnac interferometer, Mach-Zehnder interferometer, Michelson interferometer, Fabry-Perot Interferometer, lossy mode resonance, and surface-enhanced Raman scattering. Furthermore, different optical fiber techniques for detecting ultrasound in PAI are summarized. Finally, the main challenges and future development direction are briefly discussed.
Topics: Optical Fibers; Interferometry; Biosensing Techniques; Surface Plasmon Resonance; Fiber Optic Technology
PubMed: 36671899
DOI: 10.3390/bios13010064 -
Journal of Biomedical Optics Nov 2015
Topics: Cell Tracking; Holography; Interferometry; Microscopy, Phase-Contrast; Refractometry
PubMed: 26648557
DOI: 10.1117/1.JBO.20.11.111201 -
Annals of the New York Academy of... 2008The use of fluorescence radiation is of fundamental importance for tackling measurement problems in the life sciences, with recent demonstrations of probing biological... (Review)
Review
The use of fluorescence radiation is of fundamental importance for tackling measurement problems in the life sciences, with recent demonstrations of probing biological systems at the nanoscale. Usually, fluorescent light-based tools and techniques use the intensity of light waves, which is easily measured by detectors. However, the phase of a fluorescence wave contains subtle, but no less important, information about the wave; yet, it has been largely unexplored. Here, we introduce the concept of fluorescence interferometry to allow the measurement of phase information of fluorescent light waves. In principle, fluorescence interferometry can be considered a unique form of optical low-coherence interferometry that uses fluorophores as a light source of low temporal coherence. Fluorescence interferometry opens up new avenues for developing new fluorescent light-based imaging, sensing, ranging, and profiling methods that to some extent resemble interferometric techniques based on white light sources. We propose two experimental realizations of fluorescence interferometry that detect the interference pattern cast by the fluorescence fields. This article discusses their measurement capabilities and limitations and compares them with those offered by optical low-coherence interferometric schemes. We also describe applications of fluorescence interferometry to imaging, ranging, and profiling tasks and present experimental evidences of wide-field cross-sectional imaging with high resolution and large range of depth, as well as quantitative profiling with nanometer-level precision. Finally, we point out future research directions in fluorescence interferometry, such as fluorescence tomography of whole organisms and the extension to molecular interferometry by means of quantum dots and bioluminescence.
Topics: Animals; Biology; Equipment Design; Fluorescence; Fluorescent Dyes; Fourier Analysis; Humans; Interferometry; Light; Luminescence; Microscopy, Fluorescence; Microscopy, Phase-Contrast; Nanotechnology; Quantum Dots; Tomography
PubMed: 18596334
DOI: 10.1196/annals.1430.038 -
Strategies Using Bio-Layer Interferometry Biosensor Technology for Vaccine Research and Development.Biosensors Oct 2017Bio-layer interferometry (BLI) real-time, label-free technology has greatly contributed to advances in vaccine research and development. BLI Octet platforms offer... (Review)
Review
Bio-layer interferometry (BLI) real-time, label-free technology has greatly contributed to advances in vaccine research and development. BLI Octet platforms offer high-throughput, ease of use, reliability, and high precision analysis when compared with common labeling techniques. Many different strategies have been used to immobilize the pathogen or host molecules on BLI biosensors for real-time kinetics and affinity analysis, quantification, or high-throughput titer. These strategies can be used in multiple applications and shed light onto the structural and functional aspects molecules play during pathogen-host interactions. They also provide crucial information on how to achieve protection. This review summarizes some key BLI strategies used in human vaccine research and development.
Topics: Biosensing Techniques; Humans; Interferometry; Vaccines
PubMed: 29088096
DOI: 10.3390/bios7040049 -
Chemical Reviews Jul 2019Brillouin spectroscopy and imaging are emerging techniques in analytical science, biophotonics, and biomedicine. They are based on Brillouin light scattering from... (Review)
Review
Brillouin spectroscopy and imaging are emerging techniques in analytical science, biophotonics, and biomedicine. They are based on Brillouin light scattering from acoustic waves or in the GHz range, providing a nondestructive contactless probe of the mechanics on a microscale. Novel approaches and applications of these techniques to the field of biomedical sciences are discussed, highlighting the theoretical foundations and experimental methods that have been developed to date. Acknowledging that this is a fast moving field, a comprehensive account of the relevant literature is critically assessed here.
Topics: Animals; Cornea; Diagnostic Imaging; Fibroblasts; Humans; Interferometry; Lens, Crystalline; Mice; NIH 3T3 Cells; Phonons; Scattering, Radiation; Spectrum Analysis; Viscoelastic Substances
PubMed: 31042024
DOI: 10.1021/acs.chemrev.9b00019 -
Proceedings of the Japan Academy.... 2016Recent technological progress in the generation, manipulation and detection of individual single photons has opened a new scientific field of photonic quantum... (Review)
Review
Recent technological progress in the generation, manipulation and detection of individual single photons has opened a new scientific field of photonic quantum information. This progress includes the realization of single photon switches, photonic quantum circuits with specific functions, and the application of novel photonic states to novel optical metrology beyond the limits of standard optics. In this review article, the recent developments and current status of photonic quantum information technology are overviewed based on the author's past and recent works.
Topics: Algorithms; Interferometry; Light; Optics and Photonics; Photons; Physics; Quantum Theory; Technology
PubMed: 26755398
DOI: 10.2183/pjab.92.29 -
Sensors (Basel, Switzerland) Mar 2022Capillary-based backscattering interferometry has been used extensively as a tool to measure molecular binding via interferometric refractive index sensing. Previous...
Capillary-based backscattering interferometry has been used extensively as a tool to measure molecular binding via interferometric refractive index sensing. Previous studies have analysed the fringe patterns created in the backscatter direction. However, polarisation effects, spatial chirps in the fringe pattern and the practical impact of various approximations, and assumptions in existing models are yet to be fully explored. Here, two independent ray tracing approaches are applied, analysed, contrasted, compared to experimental data, and improved upon by introducing explicit polarisation dependence. In doing so, the significance of the inner diameter, outer diameter, and material of the capillary to the resulting fringe pattern and subsequent analysis are elucidated for the first time. The inner diameter is shown to dictate the fringe pattern seen, and therefore, the effectiveness of any dechirping algorithm, demonstrating that current dechirping methods are only valid for a subset of capillary dimensions. Potential improvements are suggested in order to guide further research, increase sensitivity, and promote wider applicability.
Topics: Algorithms; Interferometry; Refractometry
PubMed: 35336326
DOI: 10.3390/s22062157 -
Optics Express Nov 2022Most optical characterization methods rely on measuring the complex optical fields emerging from the interaction between light and material systems. Nevertheless,...
Most optical characterization methods rely on measuring the complex optical fields emerging from the interaction between light and material systems. Nevertheless, inherent scattering and absorption cause ambiguities in both interferometric and noninterferometric attempts to measure phase. Here we demonstrate that the complete information about a probe optical field can be encoded into the states of polarization, and develop a topography measurement method by blindly varying the ambient refractive index surrounding the sample in a wedged cuvette, which is capable of simultaneously measuring the thickness and the ambient refractive index of the sample in real time, as well as extending the measurement range of the sample thickness. With the method, we have successfully measured the topography of a 136.7 µm thick coverslip by blindly changing the ambient refractive index by 0.001246, resulting in the thickest sample characterization ever achieved by quantitative phase imaging, to the best of our knowledge. An efficient and complete characterization of optical fields is critical for any high-resolution imaging approach and the technique demonstrated here should prove attractive for applications ranging from microscopy to remote sensing. Thanks to the high precision and fast response speed, this method may pave a new way for measuring the topography of the thick samples, such as biological tissues.
Topics: Refractometry; Interferometry; Microscopy
PubMed: 36523056
DOI: 10.1364/OE.472373 -
Analytical and Bioanalytical Chemistry May 2020In this work, we review the technology of vertically interrogated optical biosensors from the point of view of engineering. Vertical sensors present several advantages... (Review)
Review
In this work, we review the technology of vertically interrogated optical biosensors from the point of view of engineering. Vertical sensors present several advantages in the fabrication processes and in the light coupling systems, compared with other interferometric sensors. Four different interrelated aspects of the design are identified and described: sensing cell design, optical techniques used in the interrogation, fabrication processes, fluidics, and biofunctionalization of the sensing surface. The designer of a vertical sensor should decide carefully which solution to adopt on each aspect prior to finally integrating all the components in a single platform. Complexity, cost, and reliability of this platform will be determined by the decisions taken on each of the design process. We focus on the research and experience acquired by our group during last years in the field of optical biosensors.
Topics: Animals; Biosensing Techniques; Equipment Design; Humans; Interferometry; Light; Optical Devices
PubMed: 32055908
DOI: 10.1007/s00216-020-02411-3