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Gastroenterology Sep 2020Alterations in the intestinal microbiota affect development of colorectal cancer and drug metabolism. We studied whether the intestinal microbiota affect the ability of...
BACKGROUND & AIMS
Alterations in the intestinal microbiota affect development of colorectal cancer and drug metabolism. We studied whether the intestinal microbiota affect the ability of aspirin to reduce colon tumor development in mice.
METHODS
We performed studies with APC mice and mice given azoxymethane and dextran sulfate sodium to induce colorectal carcinogenesis. Some mice were given antibiotics to deplete intestinal microbes, with or without aspirin, throughout the entire experiment. Germ-free mice were studied in validation experiments. Colon tissues were collected and analyzed by histopathology, quantitative reverse-transcription polymerase chain reaction, and immunoblots. Blood samples and gut luminal contents were analyzed by liquid chromatography/mass spectrometry and an arylesterase activity assay. Fecal samples were analyzed by 16S ribosomal RNA gene and shotgun metagenome sequencing.
RESULTS
Administration of aspirin to mice reduced colorectal tumor number and load in APC mice and mice given azoxymethane and dextran sulfate sodium that had been given antibiotics (depleted gut microbiota), but not in mice with intact microbiota. Germ-free mice given aspirin developed fewer colorectal tumors than conventionalized germ-free mice given aspirin. Plasma levels of aspirin were higher in mice given antibiotics than in mice with intact gut microbiota. Analyses of luminal contents revealed that aerobic gut microbes, including Lysinibacillus sphaericus, degrade aspirin. Germ-free mice fed L sphaericus had lower plasma levels of aspirin than germ-free mice that were not fed this bacterium. There was an inverse correlation between aspirin dose and colorectal tumor development in conventional mice, but this correlation was lost with increased abundance of L sphaericus. Fecal samples from mice fed aspirin were enriched in Bifidobacterium and Lactobacillus genera, which are considered beneficial, and had reductions in Alistipes finegoldii and Bacteroides fragili, which are considered pathogenic.
CONCLUSIONS
Aspirin reduces development of colorectal tumors in APC mice and mice given azoxymethane and dextran sulfate sodium, depending on the presence of intestinal microbes. L sphaericus in the gut degrades aspirin and reduced its chemopreventive effects in mice. Fecal samples from mice fed aspirin were enriched in beneficial bacteria, with reductions in pathogenic bacteria.
Topics: Adenomatous Polyposis Coli Protein; Animals; Anti-Bacterial Agents; Anticarcinogenic Agents; Aspirin; Azoxymethane; Bacillaceae; Bacteroides fragilis; Bacteroidetes; Biological Availability; Carcinogenesis; Colitis; Colon; Colorectal Neoplasms; DNA, Bacterial; Dextran Sulfate; Disease Models, Animal; Dose-Response Relationship, Drug; Feces; Gastrointestinal Microbiome; Germ-Free Life; Humans; Intestinal Mucosa; Male; Mice; Mice, Transgenic; RNA, Ribosomal, 16S
PubMed: 32387495
DOI: 10.1053/j.gastro.2020.05.004 -
Parasites & Vectors Feb 2022Emerging and re-emerging vector-borne diseases (VBDs) pose a recurring threat to tropical countries, mainly due to the abundance and distribution of the Aedes aegypti...
BACKGROUND
Emerging and re-emerging vector-borne diseases (VBDs) pose a recurring threat to tropical countries, mainly due to the abundance and distribution of the Aedes aegypti mosquito, which is a vector of the Zika, dengue, chikungunya, and yellow fever arboviruses.
METHODS
Female 3-5 day-old Ae. aegypti were distributed into two experimental groups: group I-survey of cultivable bacteria; sucrose group: fed only on sucrose, i.e., non-blood-fed (UF); blood-fed group: (i) fed with non-infected blood (BF); (ii) fed with blood infected with the Zika virus (BZIKV); (iii) pretreated with penicillin/streptomycin (pen/strep), and fed with non-infected blood (TBF); (iv) pretreated with pen/strep and fed blood infected with ZIKV, i.e., gravid with developed ovaries, (TGZIKV); group II-experimental co-infections: bacteria genera isolated from the group fed on sucrose, i.e., non-blood-fed (UF).
RESULTS
Using the cultivable method and the same mosquito colony and ZIKV strain described by in a previous work, our results reveled 11 isolates (Acinetobacter, Aeromonas, Cedecea, Cellulosimicrobium, Elizabethkingia, Enterobacter, Lysinibacillus, Pantoea, Pseudomonas, Serratia, and Staphylococcus). Enterobacter was present in all evaluated groups (i.e., UF, BF, BZIKV, TBF, and TGZIKV), whereas Elizabethkingia was present in the UF, BZIKV, and TBF groups. Pseudomonas was present in the BZIKV and TBF groups, whereas Staphylococcus was present in the TBF and TGZIKV groups. The only genera of bacteria that were found to be present in only one group were Aeromonas, Lysinibacillus, and Serratia (UF); Cedacea, Pantoea and Acinetobacter (BF); and Cellulosimicrobium (BZIKV). The mosquitoes co-infected with ZIKV plus the isolates group fed on sucrose (UF) showed interference in the outcome of infection.
CONCLUSIONS
We demonstrate that the distinct feeding aspects assessed herein influence the composition of bacterial diversity. In the co-infection, among ZIKV, Ae. aegypti and the bacterial isolates, the ZIKV/Lysinibacillus-Ae. aegypti had the lowest number of viral copies in the head-SG, which means that it negatively affects vector competence. However, when the saliva was analyzed after forced feeding, no virus was detected in the mosquito groups ZIKV/Lysinibacillus-Lu. longipalpis and Ae. aegypti; the combination of ZIKV/Serratia may interfere in salivation. This indicates that the combinations do not produce viable viruses and may have great potential as a method of biological control.
Topics: Aedes; Animals; Female; Microbiota; Mosquito Vectors; Zika Virus; Zika Virus Infection
PubMed: 35177110
DOI: 10.1186/s13071-022-05160-7 -
Journal of Fungi (Basel, Switzerland) Dec 2022Microbes are ubiquitous in the biosphere, and their therapeutic and ecological potential is not much more explored and still needs to be explored more. The are a... (Review)
Review
Microbes are ubiquitous in the biosphere, and their therapeutic and ecological potential is not much more explored and still needs to be explored more. The are a heterogeneous group of Gram-negative and Gram-positive bacteria. are dominantly found as motile, spore-forming, Gram-positive belonging to phylum and the family . species initially came into light due to their insecticidal and larvicidal properties. , a well-known insecticidal , can control many insect vectors, including a malarial vector and another, a vector that transmits infectious microbes in humans. Now its potential in the environment as a piece of green machinery for remediation of heavy metal is used. Moreover, some species of have antimicrobial potential due to the bacteriocin, peptide antibiotics, and other therapeutic molecules. Thus, this review will explore the biological disease control abilities, food preservative, therapeutic, plant growth-promoting, bioremediation, and entomopathogenic potentials of the genus .
PubMed: 36547621
DOI: 10.3390/jof8121288 -
AIMS Microbiology 2022Biomass fuel is one of the renewable energy sources that can be produced by valorization of palm oil mill effluent (POME) and empty fruit bunch (EFB). POME and EFB are...
Biomass fuel is one of the renewable energy sources that can be produced by valorization of palm oil mill effluent (POME) and empty fruit bunch (EFB). POME and EFB are available abundantly in Malaysia and only small portion is utilized to produce other value-added products. The objective of this study is to: (1) utilize the wastes from agro-industrial sector especially palm oil wastes and bio-valorize into value-added product of biomass fuel with high CEV, and simultaneously (2) reduce the waste accumulated in the palm oil factory. In this study, co-fermentation of bacteria ( sp.) and fungus () at 37 °C, 180 rpm for 5 days, followed by overnight oven-dry at 85 °C was conducted utilizing a mixture of POME and EFB with the ratio of 7:3 at laboratory scale. Three fermentation medium conditions were performed, namely: (1) Group 1: autoclaved POME and EFB without addition of any microorganisms, (2) Group 2: autoclaved POME and EFB with the addition of sp. LC 556247 and , and (3) Group 3: POME and EFB as it is (non-sterile). Among all condition, Group 2 with co-fermentation evinced the highest calorific energy value (CEV) of 26.71 MJ/kg, highest biochemical oxygen demand (BOD) removal efficiency of 61.11%, chemical oxygen demand (COD) removal efficiency at 48.47%, and total suspended solid (TSS) reduction of 37.12%. Overall, this study successfully utilized abundant POME and EFB waste and turn into value added product of renewable biomass fuel with high CEV percentage and simultaneously able to reduce abundant liquid waste.
PubMed: 36317005
DOI: 10.3934/microbiol.2022025 -
PLoS Neglected Tropical Diseases Oct 2020The microbial consortium associated with sandflies has gained relevance, with its composition shifting throughout distinct developmental stages, being strongly...
The microbial consortium associated with sandflies has gained relevance, with its composition shifting throughout distinct developmental stages, being strongly influenced by the surroundings and food sources. The bacterial components of the microbiota can interfere with Leishmania development inside the sandfly vector. Microbiota diversity and host-microbiota-pathogen interactions regarding New World sandfly species have yet to be thoroughly studied, particularly in Lutzomyia longipalpis, the primary vector of visceral leishmaniasis in Brazil.The native microbiota of different developmental stages and physiological conditions of Lu. longipalpis (Lapinha Cave), was described by culturing and 16s rRNA gene sequencing. The 16s rRNA sequencing of culture-dependent revealed 13 distinct bacterial genera (Bacillus, Enterococcus, Erwinia, Enterobacter, Escherichia, Klebsiella, Lysinibacillus, Pseudocitrobacter, Providencia, Pseudomonas, Serratia, Staphylococcus and Solibacillus). The in vitro and in vivo effects of each one of the 13 native bacteria from the Lu. longipalpis were analyzed by co-cultivation with promastigotes of L.i. chagasi, L. major, L. amazonensis, and L. braziliensis. After 24 h of co-cultivation, a growth reduction observed in all parasite species. When the parasites were co-cultivated with Lysinibacillus, all parasites of L. infantum chagasi and L. amazonensis died within 24 hours. In the in vivo co-infection of L.chagasi, L. major and L. amazonensis with the genera Lysinibacillus, Pseudocitrobacter and Serratia it was possible to observe a significant difference between the groups co-infected with the bacterial genera and the control group.These findings suggest that symbiont bacteria (Lysinibacillus, Serratia, and Pseudocitrobacter) are potential candidates for paratransgenic or biological control. Further studies are needed to identify the nature of the effector molecules involved in reducing the vector competence for Leishmania.
Topics: Animals; Bacteria; Leishmania; Microbiota; Psychodidae; RNA, Bacterial; RNA, Ribosomal, 16S
PubMed: 33052941
DOI: 10.1371/journal.pntd.0008666 -
Scientific Reports Jan 2023Aromatic compounds and metalloid oxyanions are abundant in the environment due to natural resources and industrial wastes. The high toxicity of phenol and tellurite...
Aromatic compounds and metalloid oxyanions are abundant in the environment due to natural resources and industrial wastes. The high toxicity of phenol and tellurite poses a significant threat to all forms of life. A halotolerant bacterium was isolated and identified as Lysinibacillus sp. EBL303. The remediation analysis shows that 500 mg/L phenol and 0.5 mM tellurite can be remediated entirely in separate cultures within 74 and 56 h, respectively. In addition, co-remediation of pollutants resulted in the same phenol degradation and 27% less tellurite reduction within 98 h. Since phenol and tellurite exhibited inhibitory behavior, their removal kinetics fitted well with the first-order model. In the characterization of biosynthesized tellurium nanoparticles (TeNPs), transmission electron microscopy, dynamic light scattering, FE-SEM, and dispersive X-ray (EDX) showed that the separated intracellular TeNPs were spherical and consisted of only tellurium with 22-148 nm in size. Additionally, investigations using X-ray diffraction and Fourier-transform infrared spectroscopy revealed proteins and lipids covering the surface of these amorphous TeNPs. Remarkably, this study is the first report to demonstrate the simultaneous bioremediation of phenol and tellurite and the biosynthesis of TeNPs, indicating the potential of Lysinibacillus sp. EBL303 in this matter, which can be applied to environmental remediation and the nanotechnology industry.
Topics: Tellurium; Biodegradation, Environmental; Phenol; Nanoparticles; Bacillaceae; Phenols
PubMed: 36690691
DOI: 10.1038/s41598-023-28468-5 -
Heliyon Oct 2022Xylanases have become very important enzymes in many industrial processes for the valorization of xylan-rich lignocellulosic wastes. Here, some physicochemical and...
Xylanases have become very important enzymes in many industrial processes for the valorization of xylan-rich lignocellulosic wastes. Here, some physicochemical and kinetic properties of a purified endoxylanase produced on kolanut husk-based medium by are presented. The crude enzyme solution was first subjected to precipitation with solid ammonium sulphate and further purified on DEAE-Sephadex A-50 anion-exchange and Sephadex G-100 gel filtration columns chromatography prior to biochemical characterization. The purified endoxylanase was 21 kDa as determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and was thermostable, exhibiting optimum activity at 60 °C and pH 5.0. The and were respectively estimated to be 29.5 mg/ml and 125 μmol/min/ml using Birchwood xylan as substrate. Activity of the enzyme was enhanced by Na, Ca, Mn, Mg and K at concentration of 5 mM but inhibited by Hg, Cu, Pb, Fe, EDTA, SDS and Urea. The purified endoxylanase showed high hydrolytic activity on Birchwood xylan and kolanut husk but extremely poor or no activity on carboxymethyl cellulose, starch or pectin. This strain TB7 endoxylanase has desirable properties useful for biotechnological applications in laundry, fuels, feeds, paper and pulp industries.
PubMed: 36281386
DOI: 10.1016/j.heliyon.2022.e11106 -
Toxins Jul 2021Larvicides based on the bacteria svar. (Bti) and are effective and environmentally safe compounds for the control of dipteran insects of medical importance. They... (Review)
Review
Larvicides based on the bacteria svar. (Bti) and are effective and environmentally safe compounds for the control of dipteran insects of medical importance. They produce crystals that display specific and potent insecticidal activity against larvae. Bti crystals are composed of multiple protoxins: three from the three-domain Cry type family, which bind to different cell receptors in the midgut, and one cytolytic (Cyt1Aa) protoxin that can insert itself into the cell membrane and act as surrogate receptor of the Cry toxins. Together, those toxins display a complex mode of action that shows a low risk of resistance selection. crystals contain one major binary toxin that display an outstanding persistence in field conditions, which is superior to Bti. However, the action of the Bin toxin based on its interaction with a single receptor is vulnerable for resistance selection in insects. In this review we present the most recent data on the mode of action and synergism of these toxins, resistance issues, and examples of their use worldwide. Data reported in recent years improved our understanding of the mechanism of action of these toxins, showed that their combined use can enhance their activity and counteract resistance, and reinforced their relevance for mosquito control programs in the future years.
Topics: Animals; Bacillaceae; Bacillus thuringiensis; Bacterial Toxins; Culicidae; Mosquito Control; Pest Control, Biological
PubMed: 34437394
DOI: 10.3390/toxins13080523 -
International Journal of Molecular... Apr 2022One- or two-carbon (C1 or C2) compounds have been considered attractive substrates because they are inexpensive and abundant. Methanol and ethanol are representative C1...
One- or two-carbon (C1 or C2) compounds have been considered attractive substrates because they are inexpensive and abundant. Methanol and ethanol are representative C1 and C2 compounds, which can be used as bio-renewable platform feedstocks for the biotechnological production of value-added natural chemicals. Methanol-derived formaldehyde and ethanol-derived acetaldehyde can be converted to 3-hydroxypropanal (3-HPA) via aldol condensation. 3-HPA is used in food preservation and as a precursor for 3-hydroxypropionic acid and 1,3-propanediol that are starting materials for manufacturing biocompatible plastic and polytrimethylene terephthalate. In this study, 3-HPA was biosynthesized from formaldehyde and acetaldehyde using deoxyribose-5-phosphate aldolase from (DERA) and cloned and expressed in for 3-HPA production. Under optimum conditions, DERA produced 7 mM 3-HPA from 25 mM substrate (formaldehyde and acetaldehyde) for 60 min with 520 mg/L/h productivity. To demonstrate the one-pot 3-HPA production from methanol and ethanol, we used methanol dehydrogenase from (MDH) and DERA. One-pot 3-HPA production via aldol condensation of formaldehyde and acetaldehyde from methanol and ethanol, respectively, was investigated under optimized reaction conditions. This is the first report on 3-HPA production from inexpensive alcohol substrates (methanol and ethanol) by cascade reaction using DERA and MDH.
Topics: Acetaldehyde; Escherichia coli; Ethanol; Formaldehyde; Methanol
PubMed: 35409349
DOI: 10.3390/ijms23073990 -
Frontiers in Bioengineering and... 2022Against the backdrop of towering ecological health implications of estrogen pollution and the inefficacies associated with cost-intensive treatment techniques, this...
Against the backdrop of towering ecological health implications of estrogen pollution and the inefficacies associated with cost-intensive treatment techniques, this study recorded the earliest attempt of developing an inexpensive bacterial laccase-based biocatalysts for biodegradation of EDCs (Endocrine disrupting compounds), particularly estrogens. First, a central composite design was used to investigate the interactive effects of pH (6.0-8.0), inoculum size (100-500 U/mL), and copper (Cu) (25-75 mg/L) on laccase activity and estrogen degradation respectively. Thereafter, biocatalysts was synthesized comprising laccase and glass beads or silver impregnated clay granules (SICG), which was further used to treat estrogen infused aquatic matrices under different reaction conditions. Maximum laccase activities and estrogen removal for the two tested laccases were 620 U/mL (85.8-92.9%) and 689.8 U/mL (86.8-94.6%) for BP1 and BP2, respectively, within 72 h, under conditions of optimal inoculum size and/or Cu concentration. Apart from a higher estrogen removal rate compared to free laccased, the biocatalysts were more resistant to temperature, pH and other environmental perturbations, and had enhanced storage ability and reusability. In comparison to clay, beads had a higher potential for recyclability and were more stable under certain experimental factors such as pH, reuse, and temperature, as well as storage conditions. Immobilized enzymes were able to remove 100% of E2, as well as over 90% of E1 and EE2, in 24 h, indicating that they could be scaled up to benchtop bioreactor levels.
PubMed: 36394030
DOI: 10.3389/fbioe.2022.996566