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Journal of Bacteriology Mar 1965El-Nakeeb, Moustafa A. (Rutgers, The State University, New Brunswick, N.J.), W. L. McLellan, Jr., and J. O. Lampen. Antibiotic action of griseofulvin on dermatophytes....
El-Nakeeb, Moustafa A. (Rutgers, The State University, New Brunswick, N.J.), W. L. McLellan, Jr., and J. O. Lampen. Antibiotic action of griseofulvin on dermatophytes. J. Bacteriol. 89:557-563. 1965.-The concentrations of griseofulvin required to inhibit growth and to produce the characteristic morphological distortions were determined for dermatophytes (highly sensitive), fungal plant pathogens (moderately sensitive), filamentous nonpathogenic fungi (poorly sensitive), and for yeasts and Escherichia coli (insensitive). Addition of griseofulvin to small inocula of the dermatophytes Microsporum gypseum and Trichophyton mentagrophytes produced complete and apparently permanent growth inhibition. If the antibiotic was added to actively growing cultures, the inhibition was only temporary, even with the most sensitive dermatophytes. During growth inhibition, griseofulvin temporarily halted the net synthesis of protein and nucleic acids, and of the amino acid and nucleotide pools. It decreased substantially the incorporation of C(14)-uridine or C(14)-thymidine into nucleic acids of M. gypseum, but not that of C(14)-leucine or C(14)-valine into protein. With a less sensitive culture, T. mentagrophytes x8, the uptake of uridine was inhibited only to a slight extent; the incorporation of leucine was unaffected. A partial protective effect of pruine nucleotides against growth inhibition by griseofulvin was observed with one strain of T. mentagrophytes, but not with another strain or with M. gypseum.
Topics: Amino Acids; Anti-Bacterial Agents; Arthrodermataceae; DNA; Escherichia coli; Fungi; Griseofulvin; Metabolism; Microsporum; Nucleosides; Nucleotides; Pharmacology; Proteins; RNA; Research; Thymidine; Trichophyton; Yeasts
PubMed: 14273628
DOI: 10.1128/jb.89.3.557-563.1965 -
Brazilian Journal of Microbiology :... Oct 2011Antifungal properties of some essential oils have been well documented. Clove oil is reported to have strong antifungal activity against many fungal species. In this...
Antifungal properties of some essential oils have been well documented. Clove oil is reported to have strong antifungal activity against many fungal species. In this study we have evaluated antifungal potential of essential oil of Syzygium aromaticum (L.) against some common fungal pathogens of plants and animals namely, Fusarium moniliforme NCIM 1100, Fusarium oxysporum MTCC 284, Aspergillus sp., Mucor sp., Trichophyton rubrum and Microsporum gypseum. All fungal species were found to be inhibited by the oil when tested through agar well diffusion method. Minimum inhibitory concentration (MIC) was determined for all the species. Column chromatography was performed to separate the eugenol rich fraction from clove oil. Out of seven fractions maximum activity was obtained in column fraction II. TLC and HPLC data confirmed presence of considerable Eugenol in fraction II and clove oil. Microscopic study on effect of clove oil and column fraction II on spores of Mucor sp. and M. gypseum showed distortion and shrinkage while it was absent in other column fractions. So it can be concluded that the antifungal action of clove oil is due to its high eugenol content.
PubMed: 24031751
DOI: 10.1590/S1517-83822011000400004