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Journal of Clinical Microbiology Nov 1982Microsporum equinum was isolated in Ontario, Canada, from five human and two equine cases of ringworm infection. This dermatophyte was previously recovered from North...
Microsporum equinum was isolated in Ontario, Canada, from five human and two equine cases of ringworm infection. This dermatophyte was previously recovered from North American horses on several occasions, but was considered to be M. canis. We regard M. equinum as distinct from M. canis. It can be differentiated from M. canis by the smaller size of its macroconidia, its failure to perforate hair in vitro, its poor growth and sporulation on bromocresol purple casein dextrose agar, and its incompatibility with Nannizzia otae, the telemorph of M. canis.
Topics: Animals; Culture Media; Horses; Humans; Microsporum; North America
PubMed: 7153343
DOI: 10.1128/jcm.16.5.943-947.1982 -
Journal of Clinical Microbiology Oct 2007Species of dermatophytes are classified into three anamorphic (asexual) genera, Epidermophyton, Microsporum, and Trichophyton. Conventional methods used to identify...
Species of dermatophytes are classified into three anamorphic (asexual) genera, Epidermophyton, Microsporum, and Trichophyton. Conventional methods used to identify dermatophytes are often lengthy and may be inconclusive because of atypical microscopic or colony morphology. Based on the internal transcribed spacer 1 (ITS-1) and ITS-2 sequences of the rRNA genes, an oligonucleotide array was developed to identify 17 dermatophyte species. The method consisted of PCR amplification of the ITS regions using universal primers, followed by hybridization of the digoxigenin-labeled PCR products to an array of oligonucleotides (17- to 30-mers) immobilized on a nylon membrane. Of 198 dermatophyte strains and 90 nontarget strains tested, the sensitivity and specificity of the array were 99.5% and 97.8%, respectively. The only strain not identified (Microsporum audouinii LMA 597) was found to have a nucleotide insertion at the ITS-2 region where the probe was designed. Two nontarget strains, Microsporum equinum LMA 40396666 and Trichophyton gourvilii var. intermedium CBS 170.65, were misidentified as Microsporum canis and Trichophyton soudanense, respectively. Sequence analysis of the ITS regions revealed that the two misidentified strains displayed high sequence homology with the probes designed for M. canis and T. soudanense, respectively. The present method can be used as a reliable alternative to conventional identification methods and can be completed with isolated colonies within 24 h.
Topics: Arthrodermataceae; DNA, Ribosomal Spacer; Humans; Oligonucleotide Array Sequence Analysis; Oligonucleotide Probes; Sensitivity and Specificity
PubMed: 17687010
DOI: 10.1128/JCM.00829-07 -
Advanced Biomedical Research 2020Dermatomycoses contain superficial fungal infections of keratinized layers of the body such as skin, hair, and nail that affect more than 20%-25% of people and animals...
BACKGROUND
Dermatomycoses contain superficial fungal infections of keratinized layers of the body such as skin, hair, and nail that affect more than 20%-25% of people and animals worldwide. Some fungi can cause superficial infections in animals after accidental penetration and colonization on injured skin and can be transmitted to humans by exposure. The infection caused mainly by dermatophyte species and may also be caused rarely by yeasts and nondermatophytic molds.
MATERIALS AND METHODS
Eighty-two skin scrapings and hair samples were collected from animals (sheep, cow, cat, camel, calf, goat, horse, and dog) in three specialized pet clinics and three livestock and slaughterhouses. The isolates were identified using direct microscopy, culture, and polymerase chain reaction-sequencing of ITS1-5.8SrDNA-ITS2 region.
RESULTS
Thirteen mold strains out of 82 clinical samples (15.8%) were isolated from animal lesions. ( = 4; 30.7%), ( = 2; 15.4%), ( = 2; 15.4%), ( = 1; 7.7%), ( = 1; 7.7%), ( = 1; 7.7%), ( = 1; 7.7%), and ( = 1; 7.7%) were isolated from clinical specimens.
CONCLUSION
Since opportunistic fungi are increasing as etiological agents of dermatomycoses, isolation of these molds from wounds can be a warning to veterinarians, and daily cleaning of wounds with a proper disinfectant is recommended for the prevention of fungal colonization.
PubMed: 32055538
DOI: 10.4103/abr.abr_230_19