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Materials (Basel, Switzerland) Oct 2022The present study investigated the effects of protocols for slurry preparation on the release of pollutants into bleeding water from cemented phosphogypsum (PG)...
The present study investigated the effects of protocols for slurry preparation on the release of pollutants into bleeding water from cemented phosphogypsum (PG) backfill. Backfill slurry was prepared using four different protocols in which different parameters varied, including binder/PG ratio, solid concentration, binder type and mixing procedure. The concentrations of phosphate, fluoride and sulfate and the pH values of the obtained bleeding water were measured. The results demonstrated that the slurry preparation protocols affected the quantities of pollutants through the concentrations of pollutants in bleeding water and the bleeding rate. On the one hand, the binder/PG ratio was the key factor influencing the concentrations of all pollutants in bleeding water. Comparatively speaking, the binder type and mixing procedure had an obvious influence on the fluoride concentration but had little influence on the phosphate and sulfate concentrations in the bleeding water. On the other hand, the protocols for slurry preparation affected the bleeding rate by determining the water retention and water content of the backfill slurry. The most effective protocol for slurry preparation for cemented PG backfill could reduce the bleeding rate and enhance the immobilization of pollutants, minimizing the phosphate concentration in bleeding water to below 0.2 mg/L. However, it appeared that the fluoride concentration was still tens of milligrams per liter (over the limit of 10 ten milligrams per liter), to which attention should be paid.
PubMed: 36295192
DOI: 10.3390/ma15207126 -
The Journal of Pediatrics Feb 1990We examined the relative feasibility of using random urinary dipstick testing and urinary protein/creatinine ratios in the quantitation of proteinuria. Sixty-four...
We examined the relative feasibility of using random urinary dipstick testing and urinary protein/creatinine ratios in the quantitation of proteinuria. Sixty-four children with relapsing nephrotic syndrome, ranging in age from 1 1/2 to 16 years, contributed 145 timed urine collections and 150 random specimens, which were analyzed by urinary protein dipstick, quantitation of protein and creatinine, or both. Total protein excretion was expressed as grams per surface area per day and the urinary protein/creatinine ratio as milligrams of protein per milligram of creatinine. Degrees of proteinuria were designated as physiologic (less than 0.1 gm/m2/day), intermediate (greater than 0.1 and less than 1.0 gm/m2/day), or nephrotic (greater than 1.0 gm/m2/day). The log regression analysis of the 24-hour urinary protein/creatinine ratio (y) and total protein excretion (x) was highly significant (r = 0.97; p less than 0.001). The upper and lower confidence limits of the urinary protein/creatinine ratio (1.0 and 0.1, respectively) closely approximated the designations for nephrotic and physiologic proteinuria, respectively. These values were therefore used to classify degrees of proteinuria by the urine protein/creatinine ratio. The validity of these tests was assessed by sensitivity, specificity, and predictive value, and compared with that of random testing by urinary dipstick. The urinary protein/creatinine ratio offered good reliability as a test for classifying degrees of proteinuria and accurately predicting nephrotic and physiologic proteinuria. The random dipstick testing was reliable only when results were distinctly positive and when sensitivity and specificity were low. The error in the total protein excretion value that was imposed by collection errors with high and low variations in the creatinine value (57% of samples) was largely corrected by normalization of the data by log transformation. When controlled for creatinine excretion, linear regression analysis allowed calculation of total protein excretion (TP) from the urinary protein/creatinine ratio (U P/Cr) by the following equation: TP (gm/m2/day) = 0.63 (U P/Cr) at all levels of proteinuria. The random urinary protein/creatinine ratio appeared to offer the most sensitive test for classification of proteinuria in children with nephrosis, with the advantages of ease and expediency not afforded by the 24-hour urine quantitation.
Topics: Adolescent; Child; Child, Preschool; Creatinine; Female; Humans; Infant; Male; Nephrotic Syndrome; Predictive Value of Tests; Proteinuria
PubMed: 2299494
DOI: 10.1016/s0022-3476(05)82881-1 -
Applied and Environmental Microbiology May 1986A total of 17 isolates of Stachybotrys atra isolated from various parts of Hungary and Czechoslovakia were grown on rice, and the toxin production of each isolate was...
A total of 17 isolates of Stachybotrys atra isolated from various parts of Hungary and Czechoslovakia were grown on rice, and the toxin production of each isolate was analyzed by high-performance liquid chromatography. Of the 17 isolates, 14 produced macrocyclic trichothecenes (satratoxins F, G, and H, roridin E, and verrucarin J) as well as trichoverrols A and B. Most isolates produced satratoxins G and H in higher quantities than the other trichothecenes. The yield (in milligrams) of trichothecenes produced by one isolate grown on 800 g of rice was as follows: roridin E, 12; satratoxin F, 10; satratoxin G, 75; satratoxin H, 100; trichoverrol A, 15; and trichoverrol B, 30.
Topics: Chromatography, High Pressure Liquid; Czechoslovakia; Hungary; Mitosporic Fungi; Oryza; Sesquiterpenes; Stachybotrys; Trichothecenes
PubMed: 3729393
DOI: 10.1128/aem.51.5.915-918.1986 -
Plant Physiology May 1973A purification procedure employing normal chromatographic techniques is outlined for isolating phytochrome from etiolated oat (Avena sativa L.) seedlings. Yields in...
A purification procedure employing normal chromatographic techniques is outlined for isolating phytochrome from etiolated oat (Avena sativa L.) seedlings. Yields in excess of 20% (25 milligrams or more) of phytochrome in crude extract were obtained from 10- to 15-kilograms lots. The purified oat phytochrome had an absorbance ratio (A(280) nm/A(665) nm) of 0.78 to 0.85, comparable to reported values, and gave a single major band with an estimated molecular weight of 62,000 on electrophoresis in sodium dodecyl sulfate-polyacrylamide gels. A modification of the oat isolation procedure was used to isolate phytochrome from etiolated rye Secale cereale cv. Balbo) seedlings. During isolation rye phytochrome exhibited chromatographic profiles differing from oat phytochrome on diethylaminoethyl cellulose and on molecular sieve gels. It eluted at a higher salt concentration on diethylaminoethyl cellulose and nearer the void volume on molecular sieve gels. Yields of 5 to 10% (7.5-10 milligrams) of phytochrome in crude extract were obtained from 10- to 12-kilogram seedling lots. The purified rye phytochrome had an absorbance ratio of 1.25 to 1.37, significantly lower than values in the literature and gave a single major band with an estimated molecular weight of 120,000 on electrophoresis in sodium dodecyl sulfate-polyacrylamide gels. It is suggested that the absorbance ratio and electrophoretic behavior of rye phytochrome are indices of purified native phytochrome, and that oat phytochrome as it has been described is an artifact which arises as a result of endogenous proteolysis during isolation. A rationale is provided for further modifications of the purification procedure to alleviate presumed protease contaminants.
PubMed: 16658440
DOI: 10.1104/pp.51.5.917 -
Journal of Clinical Pathology Jun 1973Using the method of Chong and Owen (1967), the normal range of methaemalbumin in plasma was 0 to 0.6 mg/100 ml, expressed as milligrams of haematin per cent. Previous...
Using the method of Chong and Owen (1967), the normal range of methaemalbumin in plasma was 0 to 0.6 mg/100 ml, expressed as milligrams of haematin per cent. Previous results, using the method of Shinowara and Walters (1963), reported a normal range of 0 to 8.0 mg/100 ml, but it was expressed as milligrams of haemoglobin percent. The conversion factor from the Shinowara method is as follows: mg haematin % = mg haemoglobin % x 0.04.
Topics: Heme; Hemeproteins; Hemoglobins; Humans; Methods; Serum Albumin
PubMed: 4718969
DOI: 10.1136/jcp.26.6.446 -
The Journal of Experimental Medicine Sep 1913In order to establish the influence of temperature upon the effect of varying doses of strychnin injected into frogs, the animals must be kept under observation for...
In order to establish the influence of temperature upon the effect of varying doses of strychnin injected into frogs, the animals must be kept under observation for several days and at various definite degrees of temperature. Statements that the animal was kept "cold," "at room temperature," or "warm" are insufficient. With a certain dose tetanus may result constantly at 30 degrees C. yet never appear at 21 degrees C., and either of these temperatures might be described as warm, when compared to a room temperature of 15 degrees C. Furthermore an animal may apparently fail to respond in the cold to an injection of certain doses of strychnin and yet be found in tetanic convulsions the next day. That an animal may have late, long lasting, or strong tetanus while kept at such a low temperature as 5 degrees C. after an injection of a dose of strychnin smaller than 0.01 of a milligram per frog emphasizes the fact that great caution must be exercised in formulating laws as to the influence of temperature on drug action. The main results of this investigation may be summarized as follows: Doses of strychnin amounting to 0.0006 of a milligram per gram of frog will cause tetanus at all temperatures between 5 degrees C. and 30 degrees C., although at low temperatures the tetanus may appear late. A dose of 0.0003 of a milligram per gram of frog will frequently produce tetanus at 5 degrees C. as well as at 30 degrees or 27 degrees C., but may nevertheless fail to produce any reaction at such an intermediary temperature as 21 degrees C. Smaller doses, 0.0002 of a milligram per gram, will cause tetanus in the cold but not at high temperatures. It may be stated in general that in frogs kept at low temperatures the tetanic state sets in later, continues longer, and each tetanic attack is of longer duration, while in the interval between the attacks the state of tonus is higher and the animals are more irritable than when they are kept at higher temperatures.
PubMed: 19867705
DOI: 10.1084/jem.18.3.300 -
Psychiatry and Clinical Neurosciences Aug 2001The aim of this study was to evaluate the effects of combination psychotropic drug treatment on heart rate variability (HRV), which was mainly controlled by the...
The aim of this study was to evaluate the effects of combination psychotropic drug treatment on heart rate variability (HRV), which was mainly controlled by the parasympathetic nervous system. Mean R-R intervals (mRR) and coefficient of variation (CV), an index of HRV, were studied in 22 psychiatric patients and 21 age- and sex-matched healthy controls. Next, in the patient group focusing on both anticholinergic and antidopaminergic properties, combination psychotropic drug daily doses were converted into biperiden milligram equivalents (BPDeq) and chlorpromazine milligram equivalents (CPZeq), respectively. The relationship between mRR and CV and these equivalent dosages was examined. A significant reduction in both mRR (P < 0.05) and CV (P < 0.05) was found in the patient group. In addition, significant negative correlations were observed between the dose of BPDeq and mRR (P < 0.05), and between the dose of BPDeq and CV (P < 0.005). In contrast, no significant correlations were observed between the dose of CPZeq and either parameter. These findings suggest that the effects of combination psychotropic drug treatment on HRV are mainly due to their anticholinergic properties. Therefore, CV is a useful indicator to assess the parasympathetic activity of psychiatric patients under combination psychotropic drug treatment.
Topics: Adult; Aged; Autonomic Nervous System; Case-Control Studies; Cholinergic Antagonists; Drug Interactions; Drug Therapy, Combination; Female; Heart Rate; Humans; Male; Mental Disorders; Middle Aged; Predictive Value of Tests; Psychotropic Drugs
PubMed: 11442884
DOI: 10.1046/j.1440-1819.2001.00873.x -
American Journal of Obstetrics and... Jun 2008Reports on pregnancies in women with glycogen storage disease type Ia (GSD-Ia) are scarce. Because of improved life expectancy, pregnancy is becoming an important issue....
OBJECTIVE
Reports on pregnancies in women with glycogen storage disease type Ia (GSD-Ia) are scarce. Because of improved life expectancy, pregnancy is becoming an important issue. We describe 15 pregnancies by focusing on dietary treatment, biochemical parameters, and GSD-Ia complications.
STUDY DESIGN
Carbohydrate requirements (milligrams per kilogram per minute), triglyceride and uric acid levels, liver ultrasonography, and creatinine clearance were investigated before, during, and after pregnancy. Data from the newborn infants were obtained from the records.
RESULTS
In the first trimester, a significant increase in carbohydrate requirements was observed (P = .007). Most patients had acceptable triglyceride and uric acid levels during pregnancy. No increase in size or number of adenomas was seen. In 3 of 4 patients, a decrease in glomerular filtration rate was observed after pregnancy. In 3 pregnancies, lactic acidosis developed during delivery with severe multiorgan failure in 1. All but 1 of the children are healthy and show good psychomotor development.
CONCLUSION
Successful pregnancies are possible in patients with GSD-Ia, although specific GSD-Ia-related risks are present.
Topics: Adult; Dietary Carbohydrates; Female; Glycogen Storage Disease Type I; Humans; Infant, Newborn; Pregnancy; Pregnancy Complications; Pregnancy Outcome; Treatment Outcome; Triglycerides; Uric Acid
PubMed: 18241814
DOI: 10.1016/j.ajog.2007.11.050 -
The New England Journal of Medicine Jun 1995Urine-concentrating ability is regulated by vasopressin. Recently, the specific water-channel protein of the renal collecting duct, known as aquaporin-2, was cloned....
BACKGROUND
Urine-concentrating ability is regulated by vasopressin. Recently, the specific water-channel protein of the renal collecting duct, known as aquaporin-2, was cloned. However, it is not certain whether this molecule is responsive to vasopressin.
METHODS
We measured the urinary excretion of aquaporin-2 and its response to vasopressin in 11 normal subjects and 9 patients with central or nephrogenic diabetes insipidus. The urine samples were collected during periods of dehydration and hydration and after the administration of vasopressin. Urine samples were analyzed for aquaporin-2 by the Western blot assay and immunogold labeling, and the amount of aquaporin-2 was determined by radioimmunoassay.
RESULTS
Aquaporin-2 was detectable in the urine in both soluble and membrane-bound forms. In the five normal subjects tested, the mean (+/- SE) urinary excretion of aquaporin-2 was 11.2 +/- 2.2 pmol per milligram of creatinine after a period of dehydration, and it decreased to 3.9 +/- 1.9 pmol per milligram of creatinine (P = 0.03) during the second hour after a period of hydration. In the six other normal subjects, an infusion of desmopressin (1-desamino-8-D-arginine vasopressin) increased the urinary excretion of aquaporin-2 from 0.8 +/- 0.3 to 11.2 +/- 1.6 pmol per milligram of creatinine (P < 0.001). The five patients with central diabetes insipidus also had increases in urinary excretion of aquaporin-2 in response to the administration of vasopressin, but the four patients with X-linked or non-X-linked nephrogenic diabetes insipidus did not.
CONCLUSIONS
Aquaporin-2 is detectable in the urine, and changes in the urinary excretion of this protein can be used as an index of the action of vasopressin on the kidney.
Topics: Adult; Aged; Aquaporin 2; Aquaporin 6; Aquaporins; Blotting, Western; Case-Control Studies; Deamino Arginine Vasopressin; Diabetes Insipidus; Female; Humans; Ion Channels; Kidney; Male; Middle Aged; Radioimmunoassay
PubMed: 7537863
DOI: 10.1056/NEJM199506083322303 -
Journal of Bacteriology Jul 1986Autocide AMI of Myxococcus xanthus was purified and shown to be a mixture of fatty acids: 46.4% saturated, 49.3% monounsaturated, and 4.3% diunsaturated. The specific...
Autocide AMI of Myxococcus xanthus was purified and shown to be a mixture of fatty acids: 46.4% saturated, 49.3% monounsaturated, and 4.3% diunsaturated. The specific autocidal activities (units per milligram) were as follows: purified AMI, 1,000; saturated fraction, 100; monounsaturated fraction, 800; diunsaturated fraction, 2,200. Model fatty acids mimicked to some extent the activity of AMI, although none of the fatty acids tested were as active as purified AMI. Spontaneous and induced mutants of M. xanthus were selected for resistance to AMI and to fatty acids. The AMI-resistant mutants were also resistant to the model fatty acids, whereas resistance to fatty acids was specific to the compound used for mutant selection. All AMI- and fatty acid-resistant mutants examined were found to be blocked in fruiting body formation. Some of these mutants were able to form normal fruiting bodies when mixed with the extracellular fluid of the parental strain. The data suggest that AMI plays a role in developmental lysis of M. xanthus.
Topics: Bacteriolysis; Fatty Acids; Mutation; Myxococcales; Surface Tension
PubMed: 3087961
DOI: 10.1128/jb.167.1.356-361.1986